Use of simulators in operative dental education: experience in southern Nigeria

Background: Though the use of simulators in operative dentistry is not new, the teaching and learning practices that take place during clinical sessions in skills laboratories are rarely reported. This study was designed to determine the current practices relating to teaching and learning of dental clinical skills in southern Nigeria.Methods: A cross-sectional study was conducted among the final year dental students in southern Nigeria using anonymous structured questionnaire as instrument for data collection. The questionnaire items included statements relating to existing teaching and learning practices. A five-point Likert scale response option was provided and descriptive summary statistics was computed.Results: There were 56 (34.8%) females and 105 (65.2%) males. Most of the students (41.0%) agreed that the theoretical concept behind clinical skills is taught prior to clinical sessions and most agreed that the objectives of each clinical session are stated and that the procedures are usually demonstrated. Most of the respondents (39.8%) agreed that feedback was sometimes embarrassing and given at the end of clinical sessions (76.6%). Equipment breakdown was a major challenge to learning.Conclusion: Dental education in the skills labs within the region appeared standard. However, feedback should be continuous and constructive. Equipment breakdown was the major constraint to learning.Key words: Simulators, Dentistry, Clinical skills laboratories, Dentistry, Simulator

Isolation of 3-(4-hydroxyphenyl)methylpropenoate and bioactivity evaluation of Gomphrena celosioides extracts

The efficacy of Gomphrena celosioides extracts in traditional medicine in the treatment of infectious diseases was evaluated by biological assays. The bioactivities of the extracts of this plant were tested against organisms. The ethyl acetate and methanol extracts of the plant displayed inhibition activities on Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Methanol extract was active against Candida albicans, Aspergillus niger and Trichophyton species with diameter zones of inhibition between 14 and 20 mm. Fractionation of the methanol extract produced 3-(4-hydroxyphenyl) ethylpropenoate with mild antimicrobial activity against the test microorganisms. The anthelmintic assay showed ethyl acetate and methanol extracts to be active against Fasciola gigantica, Taenia solium and Pheretima pasthuma. Ethyl acetate was the most toxic of the extracts causing paralysis of Taenia solium within 15 minutes and the death of Fasciola gigantica within 20 minutes of application. The brine shrimp assay gave an LC50 of 52.15 and 77.98 μg/ml on hexane and methanol extracts respectively. The result of this work corroborated the folkloric use of Gomphrena celosioides in the treatment of infectious diseases

THE COMPARATIVE ROLE OF ASCORBATE AND CHELATORS IN REVERSING OXIDATIVE STRESS, HEPATIC AND RENAL DYSFUNCTION IN SUB-ACUTE LEAD POISONING

Lead has been implicated in the induction of reactive species production, leading to organ dysfunctions. The ameliorative roles of ascorbate and chelators in acute lead poisoning were comparatively studied in thirty-five male Wistar rats (150-200g), segregated into 5 groups (n=7/Group): group 1(administered normal saline),ª¤? groups 2-5 were orally exposed to 75mg/kg body weight lead acetate (PbAc) daily for 14 days. Pre-therapy blood samples were collected to ascertain blood lead level (BLL) and catalase activity 24hours after the last PbAc exposure. Groups 3, 4, and 5 were then treated with 30mg/kg body weight D-penicillamine; 30mg/kg body weight succimer; and 500mg/kg body weight ascorbate respectively for 10 days, followed by the assay for indices of oxidative stress, hepatic and renal dysfunctions.ª¤? Results obtained showed significantly elevated BLL in the four groups exposed to PbAc. which were significantly reversed about 2 folds in groups 3-5 after therapeutic interventions. Pre-therapy blood catalase activity of the PbAc treated groups was significantly (p<0.05) reduced by 39% when compared with the control group, however ascorbate significantly (p<0.05) increased catalase activity by 2 folds above the control; decreased plasma activities of alanine transaminase and aspartate transaminase, blood urea nitrogen and creatinine among the groups administered therapeutics. These findings indicate that ascorbate is more effectiveª¤

Oral health-related quality of life: acrylic versus flexible partial dentures

Background: Teeth lost are usually replaced by all-acrylic removable partial dentures (RPD) because of its affordability and ease of fabrication. The all-acrylic RPD is said to cause significant periodontal injury and consequently affect the oral health related quality of life (OHRQoL) of denture wearers.Objective: To determine and compare OHRQoL of all-acrylic and flexible RPD wearers at baseline and after use of dentures.Design and setting: Study was quasi-experimental using a cross-over design, involving 30 patients with Kennedy class IV and anterior class III arches. Patient recruitment and review were done over an eight-month period in the Prosthetic Unit of a tertiary health facility. OHRQoL was assessed with the oral health impact profile-14 questionnaire. Data were analysed using descriptive and multivariate analysis at asignificant level of p <.05.Results: Mean age of patients was 33.8 ±10.0 years; at baseline, patient reported occasionally, fairly often or very often for 11(78.6%) OHIP items. However, after using the acrylic and flexible partial dentures, 11(36.6%) patients reported having trouble pronouncing words and 9(30.0%) found it uncomfortable to eat respectively. At baseline,there was a difference in mean OHIP scores with age (p=.02); scores reduced from 12.4 ± 9.8 to 4.8±5.3 (CI= 3.3–12.0, p=.001) after using the all-acrylic denture and 3.8±5.6 (CI= 4.3–13.0, p<.001) with the flexible denture.Conclusion: There was improvement in the OHRQoL of patients with use of flexible partial dentures. Therefore, thermoplastic materials are possible alternative RPD base materials in patient management.Keywords: OHIP-14, Removable partial dentures, Quality of Life, acrylic dentures, flexible denturesFunding: None declare

Phytochemical composition and antioxidant and antimicrobial activities of Pergularia daemia

Background: Pergularia daemia of the Asteraceae family has wide use in ethno-medicine. It is established to have pharmacologicall activity with potential medicinal significance as an antifungal, antioxidant, antimicrobial, antiinflamatory, analgesic, fertility, hepatoprotective and antic ancer agent. Aim: This study estimated the qualitative and quantitative amount of of phytochemicals in Pergularia daemia plant and the and antioxidant capacity was also determined. Setting: The fresh plants of P. Daemia were collected from Ido-Ekiti, Ekiti State, Nigeria and was identified at Plant Biology Department of the University of Ilorin, Nigeria. The samples were air-dried, pulverized and stored properly for investigation. Method: Both qualitative and quantitative phytochemical analyses of the leaves and stem were determined through n-hexane, ethyl acetate and ethanol extracts of P. daemia. Antioxidant activities of the extracts were evaluated with 2,2-diphenyl-1-picryhydroxyl (DPPH). The antibacterial and antifungal assay of the extracts were determing by dissolving 1 mg of the extract in 5 mL of the extracting solvent to give a 200 µg/mL solution. Results: Flavonoids, steroids, terpenoids and glycosides were present in all the stem and leaf extracts of P. daemia. The total phenolic content of the extracts ranged from 15.898 ± 0.111 mgGA/g to 54.679 ± 0.675 mgGA/g. The leaf has higher concentration of flavonoids than the stem and the ethanol extracts contain highest content of flavonoids. The concentration of the ethanol extract of the leaf was 400.196 mg QE/g while that of ethyl acetate and n-hexane were 388.627 mg QE/g and 338.725 mg QE/g respectively at 10 mg/mL. Ethanol extracts has the highest concentration of phenolic compounds, flavonoids and antioxidant capacity. Conclusion: The results derived from this study validate the ethno-medicinal use of the aerial parts of P. daemia

Azadirachta indica Leaf Extract Ameliorates Hyperglycemia and Hepatic Glycogenosis in Streptozotocin-induced Diabetic Wistar Rats

We studied the effects of ethanolic leaf extract of Azadirachta indica (AIE) on hepatic microscopic anatomy and oxidative stress markers in diabetic rats. Seventy-five Wistar rats (8 weeks old) were randomly assigned to five treatment groups: control; diabetic; diabetic+AIE; AIE only; and diabetic+glibenclamide. Hyperglycemia was induced in fasted rats with streptozotocin. AIE was administered orally at 500 mg/kg bw/d and glibenclamide at 600 μg/kg bw/d for 50 days (50 d). Animals were sacrificed on treatment days 7, 21 and 50. The liver was stained with PAS. Hepatic markers of oxidative stress were also estimated. At 50 d, histological study of the liver of diabetic rats showed swollen PAS+ hepatocytes, whose content was confirmed to be glycogen. On the contrary, hepatocytes of AIE-treated diabetic rats lacked glycogen. The major finding in these rats was exacerbated oxidative stress. Our findings in this model showed the beneficial effect of AIE in the amelioration of diabetic hepatic glycogenosis.Keywords: Diabetes, Azadirachta indica, hepatic glycogenosis, oxidative stress, live

Azadirachta indica Leaf Extract Ameliorates Hyperglycemia and Hepatic Glycogenosis in Streptozotocin-induced Diabetic Wistar Rats

We studied the effects of ethanolic leaf extract of Azadirachta indica (AIE) on hepatic microscopic anatomy and oxidative stress markers in diabetic rats. Seventy-five Wistar rats (8 weeks old) were randomly assigned to five treatment groups: control; diabetic; diabetic+AIE; AIE only; and diabetic+glibenclamide. Hyperglycemia was induced in fasted rats with streptozotocin. AIE was administered orally at 500 mg/kg bw/d and glibenclamide at 600 μg/kg bw/d for 50 days (50 d). Animals were sacrificed on treatment days 7, 21 and 50. The liver was stained with PAS. Hepatic markers of oxidative stress were also estimated. At 50 d, histological study of the liver of diabetic rats showed swollen PAS+ hepatocytes, whose content was confirmed to be glycogen. On the contrary, hepatocytes of AIE-treated diabetic rats lacked glycogen. The major finding in these rats was exacerbated oxidative stress. Our findings in this model showed the beneficial effect of AIE in the amelioration of diabetic hepatic glycogenosis.Keywords: Diabetes, Azadirachta indica, hepatic glycogenosis, oxidative stress, live

Vitamins C and E attenuate lipid dystrophy in tissues of rats administered aluminium

To investigate the effects of aluminum chloride (AlCl3) in the deviation of tissue lipid profiles and ways to reduce its effect using antioxidant vitamins C and E, thirty-six male albino rats (120-150g) were divided into six groups with six rats each. Group (1) received normal saline and served as control, Group (2) was administered with AlCl3 (20mg/kg body weight b.wt)), Group (3) was administered with vitamin C (200mg/kg b.wt), Group (4) was administered with vitamin E (200mg/kg b.wt), Groups (5) and (6) were administered aluminium (20mg/kg b.wt) along with vitamins C and E (200mg/kg b.wt) respectively. At the end of the experiment, blood samples and organs (liver, testis, heart, kidney and brain) were harvested and used for lipid profile determination. The results showed that oral administration of aluminum significantly (p<0.05) increased cholesterol level in plasma and VLDL+LDL and significantly decreased in erythrocyte, HDL and testis. Cholesterogenesis was induced in the brain, liver, kidney and heart. Plasma and VLDL+LDL triglyceride were significantly (p<0.05) increased while erythrocyte and brain triglyceride were significantly decreased. Plasma, VLDL+LDL and brain phospholipid levels were significantly (p<0.05) decreased and that of erythrocyte significantly increased. There was no significant difference (p>0.05) in rats supplemented with vitamin C and vitamin E compared with control. The vitamins significantly attenuated the affected lipid levels in the tissues affected. It was concluded that administration of vitamin C and vitamin E supplements may be used as therapies against the effects of Aluminium exposure on lipids.Keywords: Aluminium exposure, lipid dystrophy, vitamin C, vitamin E, tissue

Beneficial effects of omega-3 fatty acid on dyslipidemia in organs of alloxan-induced diabetic rats

Diabetes Mellitus is one of the heterogeneous metabolic disorders associated with dyslipidemia, a major risk factor contributing to cardiovascular disease. This metabolic abnormality affects virtually all organs. Over the years, antidiabetic drugs which majorly aim at the hyperglycemic aspect of the disease have been used. Therefore, to address this dyslipidemia, omega-3 fatty acid (O3FA) supplement was employed. Its effects on lipid metabolism in the organs (heart, liver, kidney, pancreas, spleen and brain) of alloxan-induced (150mg/kg body weight, intraperitoneally) diabetic male rats were investigated. O3FA (0.4g/kg b.wt/day) was administered as pre- and posttreatment for 2 weeks. The lipid levels were significantly increased (p<0.05) in diabetic rats. O3FA administration significantly reduced (p<0.05) the levels of cholesterol, phospholipids, triacylglycerol by varying extents, in the examined organs without affecting hyperglycemia in the diabetic rats. The ratio of HMG CoA/mevalonate decreased in the liver of the diabetic rats by 28% indicating increased activity of HMG-CoA reductase. This diabetes-induced dyslipidemia was accompanied by a 28% increase in the activity of hepatic HMG-CoA reductase. Administration of O3FA to the diabetic rats however resulted in 10% and 17% decrease in the activity of this enzyme in the pre- and post-treated groups respectively. Also, lipid peroxidation was significantly reduced (p<0.05) by O3FA suggesting that it has protective effect against oxidative damage. This study reveals that O3FAsupplement has beneficial effects in attenuating dyslipidemia observed in diabetes mellitus and could be beneficial as an adjunct in the management of diabetes mellitus