22 research outputs found
Evidence for stem-cell niches in the tracheal epithelium
It is generally important to elucidate airway epithelial cell lineages and to identify multipotent progenitors as targets for gene therapy. Stem (S) cells are typically present in specialized compartments spatially proximal to their differentiated progeny, but an equivalent paradigm has not been demonstrated in the airway. We discovered a distinct population of cells displaying high levels of keratin expression in murine tracheal submucosal gland ducts, and tested the hypothesis that bromodeoxyuridine (BrdU) label - retaining cells (LRCs), thought to represent the S-cells, were present in this compartment. Mice received weekly epithelial damage by intratracheal detergent or SO2 inhalation for 4 wk and received intraperitoneal injections of BrdU every 48 h during the injury and repair period. At 3 and 6 d after injury, BrdU-positive epithelial cells were noted along the entire tracheal length in both basal and lumenal cell positions. At later time points (20 and 95 d) LRCs were localized to gland ducts in the upper trachea and to systematically arrayed foci in the lower trachea, typically near the cartilage-intercartilage junction. LRCs were not pulmonary neuroendocrine cells. Heterotopic tracheal grafts after surface epithelial removal demonstrated reconstitution of a surface-like epithelium from gland remnants. These results suggest that airway epithelial S cells are localized to specific niches
Critical fluctuation conductivity in layered superconductors in strong electric field
The paraconductivity, originating from critical superconducting
order-parameter fluctuations in the vicinity of the critical temperature in a
layered superconductor is calculated in the frame of the self-consistent
Hartree approximation, for an arbitrarily strong electric field and zero
magnetic field. The paraconductivity diverges less steep towards the critical
temperature in the Hartree approximation than in the Gaussian one and it shows
a distinctly enhanced variation with the electric field. Our results indicate
that high electric fields can be effectively used to suppress order-parameter
fluctuations in high-temperature superconductors.Comment: 11 pages, 2 figures, to be published in Phys. Rev.
Mouse Chromosome 3
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46995/1/335_2004_Article_BF00648421.pd
Synthesis Of Cyclic Peptides Through An Intramolecular Amide Bond Rearrangement
[No abstract available]121421332136Greenberg, A., Breneman, C.M., Liebman, J.F., (2002) The Amide Linkage, Structural Significance in Chemistry Biochemistry and Materials Science, , Wiley, HobokenTani, K., Stoltz, B.M., (2006) Nature, 441, p. 731Richardson, J.P., Chan, C.-H., Blanc, J., Saadi, M., Macmillan, D., (2010) Org. Biomol. Chem., 8, p. 1351Kang, J., Richardson, J.P., Macmillan, D., (2009) Chem. Commun., p. 407Kang, J., Reynolds, N.L., Tyrrell, C., Dorin, J.R., Macmillan, D., (2009) Org. Biomol. Chem., 7, p. 4918Kent, S.B.H., (2009) Chem. Soc. Rev., 38, p. 338Dirksen, A., Dawson, P.E., (2008) Curr. Opin. Chem. Biol., 12, p. 760Pellois, J.-P., Muir, T.W., (2006) Curr. Opin. Chem. Biol., 10, p. 487Hackenberger, C.P.R., Schwarzer, D., (2008) Angew. Chem. 2008, 120, 10182Angew. Chem. Int. Ed., 47, p. 10030Tavassoli, A., Benkovic, S.J., (2007) Nat. Protoc., 2, p. 1126B. Premdjee, A. L. Adams, D. Macmillan, Bioorg. Med. Chem. Lett. 2011DOI:Camarero, J.A., Muir, T.W., (1997) Chem. Commun., p. 1369Shao, Y., Lu, W., Kent, S.B.H., (1998) Tetrahedron Lett., 39, p. 3911Tam, J.P., Lu, Y.-A., (1997) Tetrahedron Lett., 38, p. 5599Chen, J., Warren, J.D., Wu, B., Chen, G., Wan, Q., Danishefsky, S.J., (2006) Tetrahedron Lett., 47, p. 1969Armishaw, C.J., Dutton, L.J., Craik, D.J., Alewood, P.F., (2010) Pept. Sci., 94, p. 307Hachisu, M., Hinou, H., Takamichi, M., Tsuda, S., Koshida, S., Nishimura, S.-I., (2009) Chem. Commun., p. 1641Tulla-Puche, J., Barany, G., (2004) J. Org. Chem., 69, p. 4101Andrews, M.J.I., McInnes, C., Kontopidis, G., Innes, L., Cowan, A., Plater, A., Fischer, P.M., (2004) Org. Biomol. Chem., 2, p. 2735Schmidt, J., Garambois, V., Rocheblave, L., Martinez, J., Pèlegrin, A., Cavelier, F., Vivès, E., (2010) ChemBioChem, 11, p. 1083Clark, R.J., Craik, D.J., (2010) Pept. Sci., 94, p. 414Clark, R.J., Jensen, J., Nevin, S.T., Callaghan, B.P., Adams, D.J., Craik, D.J., (2010) Angew. Chem. 2010, 122, 6606Angew. Chem. Int. Ed., 49, p. 6545Klüver, E., Adermann, K., Schulz, A., (2006) J. Pept. Sci., 12, p. 243Lehrer, R.I., (2004) Nat. Rev. Microbiol., 2, p. 727Wu, Z., Hoover, D.M., Yang, D., Boulègue, C., Santamaria, F., Oppenheim, J.J., Lubkowski, J., Lu, W., (2003) Proc. Natl. Acad. Sci. USA, 100, p. 8880Wei, G., de Leeuw, E., Pazgier, M., Yuan, W., Zou, G., Wang, J., Ericksen, B., Lu, W., (2009) J. Biol. Chem., 284, p. 29180Taylor, K., Clarke, D.J., McCullough, B., Chin, W., Seo, E., Yang, D., Oppenheim, J., Dorin, J.R., (2008) J. Biol. Chem., 283, p. 6631Schneider, T., Kruse, T., Wimmer, R., Wiedemann, I., Sass, V., Pag, U., Jansen, A., Kristensen, H.-H., (2010) Science, 328, p. 1168Sass, V., Schneider, T., Wilmes, M., Korner, C., Tossi, A., Novikova, N., Shamova, O., Sahl, H.G., (2010) Infect. Immun., 78, p. 2793See the Supporting Information for further detailsDugourd, P., Hudgins, R.R., Clemmer, D.E., Jarrold, M.F., (1997) Rev. Sci. Instrum., 68, p. 1122Clemmer, D.E., Jarrold, M.F., (1997) J. Mass Spectrom., 32, p. 577McCullough, B.J., Kalapothakis, J., Eastwood, H., Kemper, P., MacMillan, D., Taylor, K., Dorin, J., Barran, P.E., (2008) Anal. Chem., 80, p. 6336Sewald, N., Jakubke, H.-D., (2009) Peptides: Chemistry and Biology, p. 104. , 2nd ed., Wiley-VCH, WeinheimMiyazaki, A., Tsuda, Y., Fukushima, S., Yokoi, T., Vántus, T., Bökönyi, G., Szabó, E., Okada, Y., (2008) Bioorg. Med. Chem. Lett., 18, p. 619
Proving the effectiveness of the fundamentals of robotic surgery (FRS) skills curriculum: A single-blinded, multispecialty, multi-institutional randomized control trial
Objective: To demonstrate the noninferiority of the fundamentals of robotic surgery (FRS) skills curriculum over current training paradigms and identify an ideal training platform. Summary Background Data: There is currently no validated, uniformly accepted curriculum for training in robotic surgery skills. Methods: Single-blinded parallel-group randomized trial at 12 international American College of Surgeons (ACS) Accredited Education Institutes (AEI). Thirty-three robotic surgery experts and 123 inexperienced surgical trainees were enrolled between April 2015 and November 2016. Benchmarks (proficiency levels) on the 7 FRS Dome tasks were established based on expert performance. Participants were then randomly assigned to 4 training groups: Dome (n ¼ 29), dV-Trainer (n ¼ 30), and DVSS (n ¼ 32) that trained to benchmarks and control (n ¼ 32) that trained using locally available robotic skills curricula. The primary outcome was participant performance after training based on task errors and duration on 5 basic robotic tasks (knot tying, continuous suturing, cutting, dissection, and vessel coagulation) using an avian tissue model (transfer-test). Secondary outcomes included cognitive test scores, GEARS ratings, and robot familiarity checklist scores. Results: All groups demonstrated significant performance improvement after skills training (P < 0.01). Participating residents and fellows performed tasks faster (DOME and DVSS groups) and with fewer errors than controls (DOME group; P < 0.01). Inter-rater reliability was high for the checklist scores (0.82-0.97) but moderate for GEARS ratings (0.40-0.67). Conclusions: We provide evidence of effectiveness for the FRS curriculum by demonstrating better performance of those trained following FRS compared with controls on a transfer test. We therefore argue for its implementation across training programs before surgeons apply these skills clinically. Copyright © 2019 Wolters Kluwer Health, Inc. All rights reserved