A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov.

Sixty-one strains of the root-associated nitrogen fixer Spirillum lipoferum exhibited a similar morphology in peptone--succinate salts medium: vibrioid cells having a diameter of 1.0 micrometer. When grown in broth the cells had a single polar flagellum, but when grown on agar at 30 degrees C lateral flagella of shorter wavelength were also formed. The DNA base composition was 69--71 mol% guanine + cytosine when determined by thermal denaturation. DNA homology experiments indicated the occurrence of two distinct but related homology groups: 46 strains were in group I and 15 strains were in group II. Group II strains were distinguished by their ability to use glucose as a sole carbon source for growth in nitrogen-free medium, by their production of an acidic reaction in a peptone-based glucose medium, by their requirement for biotin, and by their formation of wider, longer, S-shaped or helical cells in semisolid nitrogen-free malate medium. The results indicate that two species exist, and on the basis of their characteristics it is proposed that they be assigned to a new genus, Azospirillum. Strians belonging to group II are named A. lipoferum (Beijerinck) comb. nov., while those belonging to group I are named A. brasilense sp. nov. Strain Sp 59b (ATCC29707) is proposed as the neotype strain for A. lipoferum, and strain Sp 7 (ATCC 29145) is proposed as the type strain for A. brasilense

Bactérias diazotróficas em abacaxizeiros e bananeiras: técnicas de inoculação e monitoramento.

As bacterias diazotroficas tem sido isoladas de varias culturas, alem de leguminosas e gramineas, que tem importancia economica e social; deplantas de tuberculos como a mandioca foram isoladas bacterias do genero Burkholderia, entre outras(Balota, 1994); ja em plantas de batata-doce tem sido detectadas especies de Azospirillum(Hill et al.,1983) e Acetobacter diazophicus(Paula, 1992). Esta ultima especie também tem sido isolada de plantas de cafe(Jimenez-Salgado et al., 1998); na rizosfera de fruteiras tropicais tem sido isoladas especies de Azospirillum(Subba Rao, 1983; Ghai & Thomas, 1989). Especies deste genero e bacterias diazotroficas do tipo Herbaspirillum e Burkholderia foram tambem isoladas de raizes...bitstream/CNPAT-2010/4782/1/Dc-035.pd

Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium.

During a survey of the occurrence of Azospirillum spp. in cereal roots, we obtained 119 isolates which could not be identified as members of one of the three previously described Azospirillum species. These strains formed a very homogeneous group of N2-fixing, microaerobic, motile, vibrioid, gram-negative rod-shaped organisms which formed a veillike pellicle in semisolid medium similar to that of Azospirillum spp. However, the new isolates differed from Azospirillum spp. by their smaller cell width (0.6 to 0.7 μm), variable flagellation (one to three flagella on one or both poles), moist brownish colonies, and broader pH and oxygen tolerance for nitrogenase activity. Organic acids were the preferred carbon sources, but glucose, galactose, L-arabinose, mannitol, sorbitol, and glycerol were also used. The guanine-plus-cytosine content of the deoxyribonucleic acid was slightly lower than the guanine-plus-cytosine contents of Azospirillum spp. (66 to 67 mol%). Deoxyribonucleic acid hybridization experiments with 17 strains of the group showed 50 to 100% complementarity, while the levels of hybridization with the type strains of Azospirillum brasilense, Azospirillum lipoferum, and Azospirillum amazonense were 23, 15, and 6%, respectively. For these new isolates we propose a new genus, Herbaspirillum (the name refers to the habitat of the organisms, the roots of cereals, which are herbaceous seed-bearing plants). The type species is named Herbaspirillum seropedicae after the place where it was first isolated. The type strain is strain Z67, which has been deposited in the American Type Culture Collection as strain ATCC 3589

Ocorrência de bactérias diazotróficas e fungos micorrízicos arbusculares na cultura da mandioca

Este trabalho teve como objetivo avaliar a ocorrência, isolar e identificar fungos micorrízicos arbusculares associados à cultura da mandioca (Manihot esculenta). Amostras de solo rizosférico e de várias partes da planta (raízes, tubérculos, manivas e folhas) de locais nos Estados do Rio de Janeiro, São Paulo e Paraná, foram inoculadas nos meios LGI-P, NFb-malato e NFb-GOC, avaliando-se o número mais provável de células e a atividade de redução de acetileno. Bactérias diazotróficas foram isoladas de todas as partes da planta, com exceção das folhas, sendo identificadas como Klebsiella sp., Azospirillum lipoferum e uma bactéria denominada "E", provavelmente pertencente ao gênero Burkholderia. A Bactéria E acumulou de 7,63 mg a 14,84 mg de N/g de C em meio semi-sólido, isento de N, e conseguiu manter a capacidade de fixação biológica de N, mesmo após uma dezena de repicagens consecutivas. A colonização micorrízica variou de 31% a 69%, e a densidade de esporos de 10 a 384 esporos/100 mL de solo, predominando as espécies Entrophospora colombiana e Acaulospora scrobiculata no Rio de Janeiro, A. scrobiculata e Scutellospora heterogama no Paraná e em Piracicaba (São Paulo) e A. appendicula e S. pellucida em Campinas (São Paulo)

Quantification of Cell Movement Reveals Distinct Edge Motility Types During Cell Spreading

Actin-based motility is central to cellular processes such as migration, bacterial engulfment, and cancer metastasis, and requires precise spatial and temporal regulation of the cytoskeleton. We studied one such process, fibroblast spreading, which involves three temporal phases: early, middle, and late spreading, distinguished by differences in cell area growth. In these studies, aided by improved algorithms for analyzing edge movement, we observed that each phase was dominated by a single, kinematically and biochemically distinct cytoskeletal organization, or motility type. Specifically, early spreading was dominated by periodic blebbing; continuous protrusion occurred predominantly during middle spreading; and periodic contractions were prevalent in late spreading. Further characterization revealed that each motility type exhibited a distinct distribution of the actin-related protein VASP, while inhibition of actin polymerization by cytochalasin D treatment revealed different dependences on barbed-end polymerization. Through this detailed characterization and graded perturbation of the system, we observed that although each temporal phase of spreading was dominated by a single motility type, in general cells exhibited a variety of motility types in neighboring spatial domains of the plasma membrane edge. These observations support a model in which global signals bias local cytoskeletal biochemistry in favor of a particular motility type

Nitrogenase activity and oxygen sensitivity of the Paspalum notatum-Azotobacter paspali association

RSEP-658

Evaluation of nitrogen fertilizer and inoculation with diazotrophic bacteria on the biochemical behavior of the maize cultivar nitroflint

The evaluation of nitrogen fertilizer and inoculation with diazotrophic bacteria on the genetic and biochemical behavior of the maize (Zea mays L.) cultivar Nitroflint were performed in two experiments, one in the field and the other in greenhouse. The experimental set up consisted of a randomized blocks design with three replications and two evaluation factors, inoculation (or not) with diazotrophic bacteria and nitrogen fertilizer levels (100 kg/ha of N and 10 kg/ha of N). In the field experiment the following parameters were measured: grain production, total plant nitrogen (above ground), grain nitrogen and activities of the enzymes nitrate reductase and glutamine synthetase. In the second experiment (greenhouse), fresh weight of the plant, glutamine synthetase (transferase and synthetase) and nitrate reductase activities were determined for both leaf and root tissue. A bacterial count was made in three media, denominated LGI (semi-selective for Azospirillum amazonense), JNFb (Herbaspirillum spp.) and NFb (Azospirillum spp.) for the shoot and the root. Results of the field experiment showed an effect of N fertilizer on total N and grain production. The second experiment (greenhouse) showed an effect of N fertilizer on all parameters measured and inoculation was effective on glutamine synthetase activity of the root by transferase reaction. A correlation was obtained between root glutamine synthetase activity and bacterial growth in LGI.33696197

Deoxyribonucleic acid homology of Azospirillum amazonense Magalhães et al. 1984 and emendation of the description of the genus Azospirillum.

The results of deoxyribonucleic acid homology experiments with the type strains of Azospirillum lipoferum, Azospirillum brasilense, and Azospirillum amazonense and 19 additional strains of A. amazonense confirmed that A. amazonense is a distinct new species. The description of the genus Azospirillum is emended to accommodate A. amazonense

Self-Consistent Thermal Accretion Disk Corona Models for Compact Objects: II. Application to Cygnus X-1

We apply our self-consistent accretion disk corona (ADC) model, with two different geometries, to the broad-band X-ray spectrum of the black hole candidate Cygnus X-1. As shown in a companion paper (Dove, Wilms, and Begelman), models where the Comptonizing medium is a slab surrounding the cold accretion disk cannot have a temperature higher than about 120 keV for optical depths greater than 0.2, resulting in spectra that are much softer than the observed 10-30 keV spectrum of Cyg X-1. In addition, the slab geometry models predict a substantial ``soft excess'' at low energies, a feature not observed for Cyg X-1, and Fe K\alpha fluorescence lines that are stronger than observed. Previous Comptonization models in the literature invoke a slab geometry with the optical depth \tau_T \gta 0.3 and the coronal temperature T_c \sim 150 keV, but they are not self-consistent. Therefore, ADC models with a slab geometry are not appropriate for explaining the X-ray spectrum of Cyg X-1. Models with a spherical corona and an exterior disk, however, predict much higher self-consistent coronal temperatures than the slab geometry models. The higher coronal temperatures are due to the lower amount of reprocessing of coronal radiation in the accretion disk, giving rise to a lower Compton cooling rate. Therefore, for the sphere+disk geometry, the predicted spectrum can be hard enough to describe the observed X-ray continuum of Cyg X-1 while predicting Fe fluorescence lines having an equivalent width of \sim 40 eV. Our best-fit parameter values for the sphere+disk geometry are \tau_T \approx 1.5 and T_c \approx 90 keV.Comment: 13 pages, Latex, 10 .eps figures, uses emulateapj.sty. To be published in ApJ, October 1, 1997, Vol. 48