12 research outputs found
Substrate softness promotes terminal differentiation of human keratinocytes without altering their ability to proliferate back into a rigid environment
No full textLysargiNase mirrors trypsin for protein C-terminal and methylation-site identification
Get PDFTo improve proteome coverage and protein C-terminal identification, we characterized the Methanosarcina acetivorans thermophilic proteinase LysargiNase, which cleaves before lysine and arginine up to 55 °C. Unlike trypsin, LysargiNase-generated peptides had N-terminal lysine or arginine residues and fragmented with b ion–dominated spectra. This improved protein C terminal–peptide identification and several arginine-rich phosphosite assignments. Notably, cleavage also occurred at methylated or dimethylated lysine and arginine, facilitating detection of these epigenetic modifications
Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans
No full textSenescent synovial fibroblasts accumulate prematurely in rheumatoid arthritis tissues and display an enhanced inflammatory phenotype
No full textSingle-cell transcriptomes of the human skin reveal age-related loss of fibroblast priming
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