The Three Books on interes-bearing loans and interest (Foenus et Usurae) by Gerard Noodt, Jurist and Professor of Law

The Three Books on interes-bearing loans and interest (Foenus et Usurae) by Gerard Noodt, Jurist and Professor of Law by SJ van Niekerk, JT Pretorius, DM Kriel and DH van Zyl 2009 ISBN: 978-0-9814124-0-5 Pages: 391 Print version: Available Electronic version: Free PDF availabl

High concentrations of GTP induce conformational changes in the essential bacterial GTPase EngA and enhance its binding to the ribosome

International audienceEngA is a conserved bacterial GTPase involved in ribosome biogenesis. While essential in bacteria, EngA does not have any human orthologue and can thus be an interesting target for new antibacterial compounds. EngA is the only known GTPase bearing two G domains, making unique its catalytic cycle and the induced modulation of its conformation and interaction with the ribosome. We have investigated nucleotide-induced conformational changes in EngA in order to unveil their role in ribosome binding. SAXS and limited proteolysis were used to probe EngA confor-mational changes, and revealed a change in protein structure and a distinct rate of proteolysis induced by GTP. Structure analysis showed that the conformation adopted in solution in the presence of GTP does not match any known EngA structure, while the SAXS data measured in the presence of GDP are in perfect agreement with two crystal structures (i.e. 2HGJ and 4DCU). Combination of mass spectrometry and N-terminal sequenc-ing for the analysis of the fragmentation pattern upon proteolytic cleavage gave insights into which regions become more or less accessible in the different nucleotide-bound states. Interactions studies confirmed a stronger binding of EngA to the bacterial ribosome in the presence of GTP and suggest that the induced change in conformation of EngA plays a key role for ribosome binding

VIRUS: status and performance of the massively-replicated fiber integral field spectrograph for the upgraded Hobby-Eberly Telescope

The Visible Integral-field Replicable Unit Spectrograph (VIRUS) consists of 156 identical spectrographs (arrayed as 78 pairs, each with a pair of spectrographs) fed by 35,000 fibers, each 1.5 arcsec diameter, at the focus of the upgraded 10 m Hobby-Eberly Telescope (HET). VIRUS has a fixed bandpass of 350-550 nm and resolving power R~750. The fibers are grouped into 78 integral field units, each with 448 fibers and 20 m average length. VIRUS is the first example of large-scale replication applied to optical astronomy and is capable of surveying large areas of sky, spectrally. The VIRUS concept offers significant savings of engineering effort and cost when compared to traditional instruments. The main motivator for VIRUS is to map the evolution of dark energy for the Hobby-Eberly Telescope Dark Energy Experiment (HETDEX‡), using 0.8M Lyman-alpha emitting galaxies as tracers. The VIRUS array has been undergoing staged deployment starting in late 2015. Currently, more than half of the array has been populated and the HETDEX survey started in 2017 December. It will provide a powerful new facility instrument for the HET, well suited to the survey niche of the telescope, and will open up large spectroscopic surveys of the emission line universe for the first time. We will review the current state of production, lessons learned in sustaining volume production, characterization, deployment, and commissioning of this massive instrument.</p

Inflation of 430-parsec bipolar radio bubbles in the Galactic Centre by an energetic event

The Galactic Centre contains a supermassive black hole with a mass of four million Suns1 within an environment that differs markedly from that of the Galactic disk. Although the black hole is essentially quiescent in the broader context of active galactic nuclei, X-ray observations have provided evidence for energetic outbursts from its surroundings2. Also, although the levels of star formation in the Galactic Centre have been approximately constant over the past few hundred million years, there is evidence of increased short-duration bursts3, strongly influenced by the interaction of the black hole with the enhanced gas density present within the ring-like central molecular zone4 at Galactic longitude |l| < 0.7 degrees and latitude |b| < 0.2 degrees. The inner 200-parsec region is characterized by large amounts of warm molecular gas5, a high cosmic-ray ionization rate6, unusual gas chemistry, enhanced synchrotron emission7,8, and a multitude of radio-emitting magnetized filaments9, the origin of which has not been established. Here we report radio imaging that reveals a bipolar bubble structure, with an overall span of 1 degree by 3 degrees (140 parsecs × 430 parsecs), extending above and below the Galactic plane and apparently associated with the Galactic Centre. The structure is edge-brightened and bounded, with symmetry implying creation by an energetic event in the Galactic Centre. We estimate the age of the bubbles to be a few million years, with a total energy of 7 × 1052 ergs. We postulate that the progenitor event was a major contributor to the increased cosmic-ray density in the Galactic Centre, and is in turn the principal source of the relativistic particles required to power the synchrotron emission of the radio filaments within and in the vicinity of the bubble cavities