Perbedaan Tingkat Pengetahuan Tentang Pemanis Buatan Dalam Makanan Sebelum Dan Sesudah Mendapat Leaflet Pada Penjual Jajanan Di SD Kabupaten Klaten

Artificial sweeteners in everyday life must be considered, because it is very influential in health. Artificial sweeteners actually not prohibited but there are certain limitations in its use, therefore, this study aimed to improve the knowledge about artificial sweeteners of seller by using leaflets. The purpose of this study was to measure the level of knowledge in elementary school vending X district before and after receiving education in the form of leaflets. This research includes studies using experimental design pretest-posttest design. Sampling using cluster random sampling with a sample size of 100 respondents. This research using instruments such as questionnaires and leaflets. Data were analyzed using t-test (paired t-test). This study shows the results of the average value of the respondents 'knowledge before granting leaflet of 35.14 ± 8.00 were categorized as low and after giving leaflets respondents' knowledge increased with an average value of 69.89 ± 8.02 were included in the medium category. It can be concluded there is a difference between knowledge before and after the delivery of leaflets about artificial sweeteners that can be seen from the test results significantly p <0.05

Metabolomics meets functional assays: coupling LC-MS and microfluidic cell-based receptor-ligand analyses

IntroductionMetabolomics has become a valuable tool in many research areas. However, generating metabolomics-based biochemical profiles without any related bioactivity is only of indirect value in understanding a biological process. Therefore, metabolomics research could greatly benefit from tools that directly determine the bioactivity of the detected compounds.ObjectiveWe aimed to combine LC–MS metabolomics with a cell based receptor assay. This combination could increase the understanding of biological processes and may provide novel opportunities for functional metabolomics.MethodsWe developed a flow through biosensor with human cells expressing both the TRPV1, a calcium ion channel which responds to capsaicin, and the fluorescent intracellular calcium ion reporter, YC3.6. We have analysed three contrasting Capsicum varieties. Two were selected with contrasting degrees of spiciness for characterization by HPLC coupled to high mass resolution MS. Subsequently, the biosensor was then used to link individual pepper compounds with TRPV1 activity.ResultsAmong the compounds in the crude pepper fruit extracts, we confirmed capsaicin and also identified both nordihydrocapsaicin and dihydrocapsaicin as true agonists of the TRPV1 receptor. Furthermore, the biosensor was able to detect receptor activity in extracts of both Capsicum fruits as well as a commercial product. Sensitivity of the biosensor to this commercial product was similar to the sensory threshold of a human sensory panel.ConclusionOur results demonstrate that the TRPV1 biosensor is suitable for detecting bioactive metabolites. Novel opportunities may lie in the development of a continuous functional assay, where the biosensor is directly coupled to the LC–MS