Regulation of ige antibody production by serum molecules. III. Induction of suppressive activity by allogeneic lymphoid cell interactions and suppression of IgE synthesis by the allogeneic effect

Antibody responses of the IgE class are, like other immunoglobulin classes, regulated by a finely-tuned network of complex cellular and molecular interactions (1). Previous studies conducted in our laboratory (2, 3) have provided new insights into the differences in control mechanisms that result in individuals manifesting either the high (allergic) or low (nonallergic) IgE responder phenotype. These experiments have shown that certain manipulations (i.e. low dose X-irradiation) convert normally low responder mice to high IgE responders, apparently by diminishing a suppressor T-cell mechanism which normally dampens, rather selectively, IgE antibody production in such individuals. Similar findings have been made by Watanabe et al. (4). Recently, we have been studying the types of manipulations that could reverse the high IgE responsive state back to a low one. These studies (2, 3, 5, 6) have demonstrated that the high IgE responses induced in low responder mice can be substantially diminished, and even abolished, by passively transfusing serum or ascetic fluid from donor mice previously inoculated with mycobacterial-containing complete Freund's adjuvant (CFA). Because the suppressive activity of CFA-immune serum or ascitic fluid is so highly selective for IgE antibody production, we have recently termed these serum substances suppressive factors of allergy (SFA) (2, 3). The present study was undertaken to determine whether alternative means, particularly those that avoid administration of CFA, could be devised for the induction of SFA. Herein, we report the effectiveness of allogeneic lymphoid cell interactions in inducing SFA, both in vivo and in vitro, as well as the potent suppressive effects of an in vivo allogeneic effect on irradiation enhanced IgE antibody production in low responder mice

The clustering of high-redshift galaxies in the cold dark matter scenario

We investigate the clustering of high-redshift galaxies in five variants of the cold dark matter (CDM) scenario, using hydrodynamic cosmological simulations that resolve the formation of systems with circular velocities vc ≥ 100 km s-1 (Ω = 1) or vc ≥ 70 km s-1 (Ω = 0.4). Although the five models differ in their cosmological parameters and in the shapes and amplitudes of their mass power spectra, they predict remarkably similar galaxy clustering at z = 2, 3, and 4. The galaxy correlation functions show almost no evolution over this redshift range, even though the mass correlation functions grow steadily in time. Despite the fairly low circular velocity threshold of the simulations, the high-redshift galaxies are usually highly biased tracers of the underlying mass distribution; the bias factor evolves with redshift and varies from model to model. Predicted correlation lengths for the resolved galaxy population are 2-3 h-1 Mpc (comoving) at z = 3. More massive galaxies tend to be more strongly clustered. These CDM models have no difficulty in explaining the strong observed clustering of Lyman-break galaxies, and some may even predict excessive clustering. Because the effects of bias obscure differences in mass clustering, it appears that Lyman-break galaxy clustering will not be a good test of cosmological models but will instead provide a tool for constraining the physics of galaxy formation

X-ray absorption by the low-redshift intergalactic medium: A numerical study of the Lambda cold dark matter model

Using a hydrodynamic simulation of a cold dark matter universe with a cosmological constant, we investigate the X-ray forest absorption imprinted on the spectra of background quasars by the intervening intergalactic medium (IGM), at redshift z 0. In agreement with previous studies, we find that O VII and O VIII produce the strongest absorption features. The strong oxygen absorbers that might be detectable with Chandra or XMM-Newton arise in gas with T ~ 105.5-106.5 K and overdensities δ 100 that are characteristic of galaxy groups. Future X-ray missions could detect weaker oxygen absorption produced by gas with a wider range of temperatures and the lower densities of unvirialized structures; they could also detect X-ray forest absorption by carbon, nitrogen, neon, iron, and possibly silicon. If the IGM metallicity is Z = 0.1 Z, as we assume in most of our calculations, then the predicted number of systems strong enough for a ~5 σ detection with Chandra or XMM-Newton is extremely low. However, scatter in metallicity increases the number of strong absorbers even if the mean metallicity remains the same, making the predictions somewhat more optimistic. Our simulation reproduces the high observed incidence of O VI (λλ1032, 1038) absorbers, and the most promising strategy for finding the X-ray forest is to search at the redshifts of known O VI systems, thus reducing the signal-to-noise ratio threshold required for a significant detection. However, while many O VI absorbers have associated O VII or O VIII absorption, the O VI systems trace only the low-temperature phases of the X-ray forest, and a full accounting of the strong O VII and O VIII systems will require a mission with the anticipated capabilities of Constellation-X. The large effective area of the XEUS satellite would make it an extremely powerful instrument for studying the IGM, measuring X-ray forest absorption by a variety of elements, and revealing the shock-heated filaments that may be an important reservoir of cosmic baryons

Lyman break galaxies and the Ly alpha forest

We use hydrodynamic cosmological simulations to predict correlations between Lyα forest absorption and the galaxy distribution at redshift z 3. The probability distribution function (PDF) of Lyα flux decrements shifts systematically toward higher values in the vicinity of galaxies, reflecting the overdense environments in which these galaxies reside. The predicted signal remains strong in spectra smoothed over 50-200 km s-1, allowing tests with moderate-resolution quasar spectra. The strong bias of high-redshift galaxies toward high-density regions imprints a clear signature on the flux PDF, but the predictions are not sensitive to galaxy baryon mass or star formation rate, and they are similar for galaxies and dark matter halos. The dependence of the flux PDF on galaxy proximity is sensitive to redshift determination errors, with rms errors of 150-300 km s-1 substantially weakening the predicted trends. On larger scales, the mean galaxy overdensity in a cube of 5 or 10 h-1 Mpc (comoving) is strongly correlated with the mean Lyα flux decrement on a line of sight through the cube center. The slope of the correlation is ~3 times steeper for galaxies than for dark matter as a result of galaxy bias. The predicted large-scale correlation is in qualitative agreement with recently reported observational results. However, observations also show a drop in the average absorption in the immediate vicinity of galaxies, which our models do not predict even if we allow the galaxies or active galactic nuclei within them to be ionizing sources. This decreased absorption could be a signature of galaxy feedback on the surrounding intergalactic medium, perhaps via galactic winds. We find that a simplified wind model that eliminates neutral hydrogen in spheres around the galaxies can marginally explain the data. However, because peculiar velocities allow gas at large distances to produce saturated absorption at the galaxy redshift, these winds (or any other feedback mechanism) must extend to comoving radii of ~1.5 h-1 Mpc to reproduce the observations. We also discuss the possibility that extended Lyα emission from the target galaxies fills in the expected Lyα forest absorption at small angular separations

Closing in on Omega(M): The amplitude of mass fluctuations from galaxy clusters and the Ly alpha forest

We estimate the present-day value of the matter density parameter ΩM by combining constraints from the galaxy cluster mass function with Croft et al.\u27s recent measurement of the mass power spectrum, P(k), from Lyα forest data. The key assumption of the method is that cosmic structure formed by gravitational instability from Gaussian primordial fluctuations. For a specified value of ΩM, matching the observed cluster mass function then fixes the value of σ8, the rms amplitude of mass fluctuations in 8 h-1 Mpc spheres, and it thus determines the normalization of P(k) at z = 0. The value of ΩM also determines the ratio of P(k) at z = 0 to P(k) at z = 2.5, the central redshift of the Lyα forest data; the ratio is different for an open universe (Λ = 0) or a flat universe. Because the Lyα forest measurement only reaches comoving scales 2π/k ~ 15-20 h-1 Mpc, the derived value of ΩM depends on the value of the power spectrum shape parameter Γ, which determines the relative contribution of larger scale modes to σ8. Adopting Γ = 0.2, a value favored by galaxy clustering data, we find ΩM = 0.46+0.12-0.10 for an open universe and ΩM = 0.34+0.13-0.09 for a flat universe (1 σ errors, not including the uncertainty in cluster normalization). Cluster-normalized models with ΩM = 1 predict too low an amplitude for P(k) at z = 2.5, while models with ΩM = 0.1 predict too high an amplitude. The more general best-fit parameter combination is ΩM + 0.2ΩΛ 0.46 + 1.3(Γ - 0.2), where ΩΛ ≡ Λ/3H20. Analysis of larger, existing samples of QSO spectra could greatly improve the measurement of P(k) from the Lyα forest, allowing a determination of ΩM by this method with a precision of ~15%, limited mainly by uncertainty in the cluster mass function

Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex

The results of this study provide compelling evidence for the existence of the gene or genes controlling optimal T-B-cell cooperative interactions in the designated I region of the H-2 gene complex. Previously, we have speculated that the relevant gene(s) involved may well be located in this region based on several observations from our earlier work in this area (3, 5, 6). Thus, in the preceding paper, we showed that T and B cells from B10.BR and A strain mice developed effective cooperative interactions in vitro to DNP-KLH in a system identical to the one reported herein. Since these mice differ for genes in the S and D regions of H-2 but are identical for K and I region genes, we were able to localize the critical genes to the K-end of H-2

The growth of central and satellite galaxies in cosmological smoothed particle hydrodynamics simulations

We examine the accretion and merger histories of central and satellite galaxies in a smoothed particle hydrodynamics (SPH) cosmological simulation that resolves galaxies down to 7 × 109 M⊙. Most friends-of-friends haloes in the simulation have a distinct central galaxy, typically 2–5 times more massive than the most massive satellite. As expected, satellites have systematically higher assembly redshifts than central galaxies of the same baryonic mass, and satellites in more massive haloes form earlier. However, contrary to the simplest expectations, satellite galaxies continue to accrete gas and convert it to stars; the gas accretion declines steadily over a period of 0.5–1 Gyr after the satellite halo merges with a larger parent halo. Satellites in a cluster mass halo eventually begin to lose baryonic mass. Typically, satellites in our simulation are 0.1–0.2 mag bluer than in models that assume no gas accretion on to satellites after a halo merger. Since z= 1, 27 per cent of central galaxies (above 3 × 1010 M⊙) and 22 per cent of present-day satellite galaxies have merged with a smaller system above a 1:4 mass ratio; about half of the satellite mergers occurred after the galaxy became a satellite and half before. In effect, satellite galaxies can remain ‘central’ objects of halo substructures, with continuing accretion and mergers, making the transition in assembly histories and physical properties a gradual one. Implementing such a gradual transformation in semi-analytic models would improve their agreement with observed colour distributions of satellite galaxies in groups and with the observed colour dependence of galaxy clustering

Constraints on cosmological parameters from the Ly alpha forest power spectrum and COBE DMR

We combine COBE DMR measurements of cosmic microwave background (CMB) anisotropy with a recent measurement of the mass power spectrum at redshift z = 2.5 from Lyα forest data to derive constraints on cosmological parameters and test the inflationary cold dark matter (CDM) scenario of structure formation. By treating the inflationary spectral index n as a free parameter, we are able to find successful fits to the COBE and Lyα forest constraints in Ωm = 1 models with and without massive neutrinos and in low-Ωm models with and without a cosmological constant. Within each class of model, the combination of COBE and the Lyα forest P(k) constrains a parameter combination of the form ΩmhαnβΩ, with different indices for each case. This new constraint breaks some of the degeneracies in cosmological parameter determinations from other measurements of large-scale structure and CMB anisotropy. The Lyα forest P(k) provides the first measurement of the slope of the linear mass power spectrum on ~Mpc scales, ν = -2.25 ± 0.18, and it confirms a basic prediction of the inflationary CDM scenario: an approximately scale invariant spectrum of primeval fluctuations (n 1) modulated by a transfer function that bends P(k) toward kn-4 on small scales. Considering additional observational data, we find that COBE-normalized, Ωm = 1 models that match the Lyα forest P(k) do not match the observed masses of rich galaxy clusters, and that low-Ωm models with a cosmological constant provide the best overall fit to the available data, even without the direct evidence for cosmic acceleration from Type Ia supernovae. With our fiducial parameter choices, the flat, low-Ωm models that match COBE and the Lyα forest P(k) also match recent measurements of small-scale CMB anisotropy. Modest improvements in the Lyα forest P(k) measurement could greatly restrict the allowable region of parameter space for CDM models, constrain the contribution of tensor fluctuations to CMB anisotropy, and achieve a more stringent test of the current consensus model of structure formation