Ofertas de suplementos múltiplos em tourinhos anelorados na fase de recria, em pastagens durante o período da seca: produção e eficiência microbiana.

O uso de suplementos múltiplos para bovinos visa aumentar o aporte de nutrientes, para melhor atender às exigências nutricionais. Neste contexto, avaliou-se a digestibilidade em tourinhos anelorados em fase de recria, recebendo diferentes ofertas de suplementos em pastagem de Brachiaria decumbens. A área foi dividida em cinco piquetes de 2,0 ha cada. Foram utilizados 30 tourinhos, com peso e idade iniciais médios de 230,0 ±6,14 kg e 8,5 ±0,18 meses, em delineamento inteiramente casualizado, contendo cinco tratamentos, sendo quatro ofertas de suplementos, mais mistura mineral (MM) e seis repetições. Foram avaliados a MM (60g/animal) e suplementos múltiplos, formulados para atender diferentes níveis de suplementação diariamente nas quantidades de 0,5, 1,0, 1,5 e 2,0 kg/animal e suprir 300g/dia de PB. O consumo de suplemento aumentou (P<0,10) a produção de proteína microbiana (PMic), quando comparado com os animais do grupo controle. A produção de PMic apresentou comportamento quadrático com ponto de máxima produção de 404,47 gramas na quantidade de 1,34kg de suplemento. A eficiência de síntese microbiana (EFMic) não diferiu entre os animais do grupo controle e os suplementados. Contudo, observa-se um comportamento quadrático com máxima eficiência 160,38 g/kg de NDT para oferta de 1,18 kg de suplemento. Observa-se que o ponto (1,18 kg) onde ocorreu a máxima EFMic foi inferior ao valor (1,34 kg) em que ocorreu a máxima produção de PMic. Conclui-se que, o ponto ótimo entre produção e eficiência de síntese de proteína microbiana, está entre as ofertas de suplemento diárias de 1,18 e 1,34 kg/animal

Evaluation of acidogenesis products’ effect on biogas production performed with metagenomics and isotopic approaches

Background: During the acetogenic step of anaerobic digestion, the products of acidogenesis are oxidized to substrates for methanogenesis: hydrogen, carbon dioxide and acetate. Acetogenesis and methanogenesis are highly interconnected processes due to the syntrophic associations between acetogenic bacteria and hydrogenotrophic methanogens, allowing the whole process to become thermodynamically favorable. The aim of this study is to determine the influence of the dominant acidic products on the metabolic pathways of methane formation and to find a core microbiome and substrate-specific species in a mixed biogas-producing system. Results: Four methane-producing microbial communities were fed with artificial media having one dominant component, respectively, lactate, butyrate, propionate and acetate, for 896 days in 3.5-L Up-flow Anaerobic Sludge Blanket (UASB) bioreactors. All the microbial communities showed moderately different methane production and utilization of the substrates. Analyses of stable carbon isotope composition of the fermentation gas and the substrates showed differences in average values of δ13C(CH4) and δ13C(CO2) revealing that acetate and lactate strongly favored the acetotrophic pathway, while butyrate and propionate favored the hydrogenotrophic pathway of methane formation. Genome-centric metagenomic analysis recovered 234 Metagenome Assembled Genomes (MAGs), including 31 archaeal and 203 bacterial species, mostly unknown and uncultivable. MAGs accounted for 54%–67% of the entire microbial community (depending on the bioreactor) and evidenced that the microbiome is extremely complex in terms of the number of species. The core microbiome was composed of Methanothrix soehngenii (the most abundant), Methanoculleus sp., unknown Bacteroidales and Spirochaetaceae. Relative abundance analysis of all the samples revealed microbes having substrate preferences. Substrate-specific species were mostly unknown and not predominant in the microbial communities. Conclusions: In this experimental system, the dominant fermentation products subjected to methanogenesis moderately modified the final effect of bioreactor performance. At the molecular level, a different contribution of acetotrophic and hydrogenotrophic pathways for methane production, a very high level of new species recovered, and a moderate variability in microbial composition depending on substrate availability were evidenced. Propionate was not a factor ceasing methane production. All these findings are relevant because lactate, acetate, propionate and butyrate are the universal products of acidogenesis, regardless of feedstock

A 27 day persistence model of near-Earth solar wind conditions: a long lead-time forecast and a benchmark for dynamical models

Geomagnetic activity has long been known to exhibit approximately 27 day periodicity, resulting from solar wind structures repeating each solar rotation. Thus a very simple near-Earth solar wind forecast is 27 day persistence, wherein the near-Earth solar wind conditions today are assumed to be identical to those 27 days previously. Effective use of such a persistence model as a forecast tool, however, requires the performance and uncertainty to be fully characterized. The first half of this study determines which solar wind parameters can be reliably forecast by persistence and how the forecast skill varies with the solar cycle. The second half of the study shows how persistence can provide a useful benchmark for more sophisticated forecast schemes, namely physics-based numerical models. Point-by-point assessment methods, such as correlation and mean-square error, find persistence skill comparable to numerical models during solar minimum, despite the 27 day lead time of persistence forecasts, versus 2–5 days for numerical schemes. At solar maximum, however, the dynamic nature of the corona means 27 day persistence is no longer a good approximation and skill scores suggest persistence is out-performed by numerical models for almost all solar wind parameters. But point-by-point assessment techniques are not always a reliable indicator of usefulness as a forecast tool. An event-based assessment method, which focusses key solar wind structures, finds persistence to be the most valuable forecast throughout the solar cycle. This reiterates the fact that the means of assessing the “best” forecast model must be specifically tailored to its intended use

4pi Models of CMEs and ICMEs

Coronal mass ejections (CMEs), which dynamically connect the solar surface to the far reaches of interplanetary space, represent a major anifestation of solar activity. They are not only of principal interest but also play a pivotal role in the context of space weather predictions. The steady improvement of both numerical methods and computational resources during recent years has allowed for the creation of increasingly realistic models of interplanetary CMEs (ICMEs), which can now be compared to high-quality observational data from various space-bound missions. This review discusses existing models of CMEs, characterizing them by scientific aim and scope, CME initiation method, and physical effects included, thereby stressing the importance of fully 3-D ('4pi') spatial coverage.Comment: 14 pages plus references. Comments welcome. Accepted for publication in Solar Physics (SUN-360 topical issue

Newly-discovered interactions between bacteriophages and the process of calcium carbonate precipitation

Although it has been suggested that viruses may induce precipitation of carbonate, the exact mechanisms involved and the roles they might play in the nucleation of carbonates are still unknown. Experimental evidence is reported here demonstrating that bacteriophages are an important factor in calcium carbonate formation. Short-term experiments were designed to induce viral-mediated mineral precipitation at different fluid compositions. The precipitates obtained, analysed with fluorescence and electron microscopes and X-Ray diffraction, and evaluated by statistics, clearly show that bacteriophages strongly affect the process of carbonate precipitation. We demonstrate that as a result of the coalescence of the phages, the carbonate precipitates form large aggregates consisting of spheroids. The spheroids are constructed of angular nanoparticles resembling viral-like particles. In control experiments spheroids were also observed; however, they consist of irregular nanoparticles and additionally they do not form large aggregates. We also demonstrate that bacteriophages strongly influence the mineral composition of the precipitates, with vaterite being the dominant form, and they likely control vaterite stability