Direct Differential Photometric Stereo Shape Recovery of Diffuse and Specular Surfaces

This is the author accepted manuscript. The final version is available from Springer via http://dx.doi.org/10.1007/s10851-016-0633-0Recovering the 3D shape of an object from shading is a challenging problem due to the complexity of modeling light propagation and surface reflections. Photometric Stereo (PS) is broadly considered a suitable approach for high-resolution shape recovery, but its functionality is restricted to a limited set of object surfaces and controlled lighting setup. In particular, PS models generally consider reflection from objects as purely diffuse, with specularities being regarded as a nuisance that breaks down shape reconstruction. This is a serious drawback for implementing PS approaches, since most common materials have prominent specular components. In this paper, we propose a PS model that solves the problem for both diffuse and specular components aimed at shape recovery of generic objects with the approach being independent of the albedo values thanks to the image ratio formulation used. Notably, we show that by including specularities, it is possible to solve the PS problem for a minimal number of three images using a setup with three calibrated lights and a standard industrial camera. Even if an initial separation of diffuse and specular components is still required for each input image, experimental results on synthetic and real objects demonstrate the feasibility of our approach for shape reconstruction of complex geometries.The first author acknowledges the support of INDAM under the GNCS research Project “Metodi numerici per la regolarizzazione nella ricostruzione feature-preserving di dati.

Machine Learning for Cultural Heritage: A Survey

The application of Machine Learning (ML) to Cultural Heritage (CH) has evolved since basic statistical approaches such as Linear Regression to complex Deep Learning models. The question remains how much of this actively improves on the underlying algorithm versus using it within a ‘black box’ setting. We survey across ML and CH literature to identify the theoretical changes which contribute to the algorithm and in turn them suitable for CH applications. Alternatively, and most commonly, when there are no changes, we review the CH applications, features and pre/post-processing which make the algorithm suitable for its use. We analyse the dominant divides within ML, Supervised, Semi-supervised and Unsupervised, and reflect on a variety of algorithms that have been extensively used. From such an analysis, we give a critical look at the use of ML in CH and consider why CH has only limited adoption of ML

Seeing the sound: a new multimodal imaging device for computer vision

Audio imaging can play a fundamental role in computer vision, in particular in automated surveillance, boosting the accuracy of current systems based on standard optical cameras. We present here a new hybrid device for acousticoptic imaging, whose characteristics are tailored to automated surveillance. In particular, the device allows realtime, high frame rate generation of an acoustic map, overlaid over a standard optical image using a geometric calibration of audio and video streams. We demonstrate the potentialities of the device for target tracking on three challenging setup showing the advantages of using acoustic images against baseline algorithms on image tracking. In particular, the proposed approach is able to overcome, often dramatically, visual tracking with state-of-art algorithms, dealing efficiently with occlusions, abrupt variations in visual appearence and camouflage. These results pave the way to a widespread use of acoustic imaging in application scenarios such as in surveillance and security

Clinical Effects of the Extract of the Seeds of the Indian Celery-Apium Graveolens-In Horses Affected by Chronic Osteoarthritis.

The extract of the seeds from Indian celery, Apium greaveolens (CSE), tested in experimental animals (rodents), and in humans aected by chronic osteoarthritic diseases, exhibits anti-inflammatory eects that can be compared, to some degree, to those of non-steroid anti-inflammatory drugs (NSAID). In view of a potential use of CSE in the equine species, it was tested on horses aected by chronic articular pathologies. The trial was performed on 20 horses divided into three dierent groups, orally treated with 0 (controls), 7.0 or 30 g of CSE BID. Basic orthopedic examinations were conducted, vital signs were observed, and blood samples collected. Improvement was observed at the highest dosage tested (30 g of CSE BID), as reflected in the score values of three clinical parameters, (i) amplitude and (ii) sensitivity to passive flexion and (iii) flexion test. Since the improvement of these parameters can be correlated with a lower perception of the pain, the present data suggest that the CSE treatment can have an analgesic eect in horses aected by chronic osteoarthritic diseases

Canine mesenchymal cell lyosecretome production and safety evaluation after allogenic intraarticular injection in osteoarthritic dogs

In recent years, mesenchymal stromal cells (MSCs) have shown promise as a therapy in treating musculoskeletal diseases, and it is currently believed that their therapeutic effect is mainly related to the release of proteins and extracellular vesicles (EVs), known as secretome. In this work, three batches of canine MSC-secretome were prepared by standardized processes according to the current standard ISO9001 and formulated as a freeze-dried powder named Lyosecretome. The final products were char-acterized in protein and lipid content, EV size distribution and tested to ensure the microbiological safety required for intraarticular injection. Lyosecretome induced the proliferation of adipose tissue-derived canine MSCs, tenocytes, and chondrocytes in a dose-dependent manner and showed anti-elastase activ-ity, reaching 85% of inhibitory activity at a 20 mg/mL concentration. Finally, to evaluate the safety of the preparation, three patients affected by bilateral knee or elbow osteoarthritis were treated with two intraarticular injections (t = 0 and t = 40 days) of the allogeneic Lyosecretome (20 mg corresponding 2 × 106 cell equivalents) resuspended in hyaluronic acid in one joint and placebo (mannitol resuspended in hyalu-ronic acid) in the other joint. To establish the safety of the treatment, the follow-up included a questionnaire addressed to the owner and orthopaedic examinations to assess lameness grade, pain score, func-tional disability score and range of motion up to day 80 post-treatment. Overall, the collected data suggest that intra-articular injection of allogeneic Lyosecretome is safe and does not induce a clinically significant local or systemic adverse response

Equine mesenchymal stem/stromal cells freeze-dried secretome (Lyosecretome) for the treatment of musculoskeletal diseases: Production process validation and batch release test for clinical use

In the last decades, it has been demonstrated that the regenerative therapeutic efficacy of mesenchymal stromal cells is primarily due to the secretion of soluble factors and extracellular vesicles, collectively known as secretome. In this context, our work described the preparation and characterization of a freeze-dried secretome (Lyosecretome) from adipose tissue-derived mesenchy-mal stromal cells for the therapy of equine musculoskeletal disorder. An intraarticular injectable pharmaceutical powder has been formulated, and the technological process has been validated in an authorized facility for veterinary clinical-use medicinal production. Critical parameters for quality control and batch release have been identified regarding (i) physicochemical properties; (ii) extracellular vesicle morphology, size distribution, and surface biomarker; (iii) protein and lipid content; (iv) requirements for injectable pharmaceutical dosage forms such as sterility, bacterial endotoxin, and Mycoplasma; and (v) in vitro potency tests, as anti-elastase activity and proliferative activity on musculoskeletal cell lines (tenocytes and chondrocytes) and mesenchymal stromal cells. Finally, proteins putatively responsible for the biological effects have been identified by Lyosecretome pro-teomic investigation: IL10RA, MXRA5, RARRES2, and ANXA1 modulate the inflammatory process RARRES2, NOD1, SERPINE1, and SERPINB9 with antibacterial activity. The work provides a proof-of-concept for the manufacturing of clinical-grade equine freeze-dried secretome, and prototypes are now available for safety and efficacy clinical trials in the treatment of equine musculoskeletal diseases

Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors

<p>Abstract</p> <p>Background</p> <p>Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manipulation of Equine Adipose-Derived Stromal Cells has been investigated.</p> <p>Results</p> <p>Equine Adipose-Derived Stromal Cells were successfully virally transduced as well as transiently and stably transfected with appropriate parameters, without detrimental effect on their differentiation properties. Moreover, green fluorescent protein alone, fused to <it>neo </it>gene, or co-expressed as bi-cistronic reporter constructs, driven by viral and house-keeping gene promoters, were tested. The better expressed cassette was employed to stably transfect Adipose-Derived Stromal Cells for cell therapy purposes. Stably transfected Equine Adipose-Derived Stromal Cells with a heterologous secreted viral antigen were able to immunize horses upon injection into the lateral wall of the neck.</p> <p>Conclusion</p> <p>This study provides the methods to successfully transgenize Adipose-Derived Stromal Cells both by lentiviral vector and by transfection using optimized constructs with suitable promoters and reporter genes. In conclusion these findings provide a working platform for the delivery of potentially therapeutic proteins to the site of cells injection via transgenized Equine Adipose-Derived Stromal Cells.</p