Supercritical fluid coating of API on excipient enhances drug release

A process to coat particles of active pharmaceutical ingredient (API) onto microcrystalline cellulose (MCC) excipient shows promise as a new way to dosage forms showing enhanced drug release. The process consists of a fluidized bed operated at elevated pressure in which API particles are precipitated from a Supercritical Anti-Solvent process (SAS). MCC particles were used as an excipient in the fluidized bed and collect the SAS-generated API particles. Naringin was selected as the model API to coat onto MCC. A number of operational parameters of the process were investigated: fluidization velocity, coating pressure, temperature, concentration of drug solution, drug solution flow rate, drug mass, organic solvent, MCC mass and size and CO2-to-organic solution ratio. SEM and SPM analyses showed that the MCC particle surfaces were covered with near-spherical nanoparticles with a diameter of approximately 100–200 nm, substantially smaller than the as-received API material. XRD showed that naringin changed from crystalline to amorphous during processing. The coated particles resulting from the SAS fluidized bed process have a higher loading of API, gave faster release rates and higher release ratios in comparison with those produced using a conventional fluidized bed coating process. The approach could be transferred to other industries where release is important such as agrochemical, cosmetic and food

Functional Organization of Social Perception and Cognition in the Superior Temporal Sulcus

The superior temporal sulcus (STS) is considered a hub for social perception and cognition, including the perception of faces and human motion, as well as understanding others' actions, mental states, and language. However, the functional organization of the STS remains debated: Is this broad region composed of multiple functionally distinct modules, each specialized for a different process, or are STS subregions multifunctional, contributing to multiple processes? Is the STS spatially organized, and if so, what are the dominant features of this organization? We address these questions by measuring STS responses to a range of social and linguistic stimuli in the same set of human participants, using fMRI. We find a number of STS subregions that respond selectively to certain types of social input, organized along a posterior-to-anterior axis. We also identify regions of overlapping response to multiple contrasts, including regions responsive to both language and theory of mind, faces and voices, and faces and biological motion. Thus, the human STS contains both relatively domain-specific areas, and regions that respond to multiple types of social information.David & Lucile Packard FoundationNational Science Foundation (U.S.). Graduate Research FellowshipNational Science Foundation (U.S.) (CCF-1231216

Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media.

Due to their immunosuppressive properties, mesenchymal stem cells (MSC) have been evaluated for the treatment of immunological diseases. However, the animal-derived growth supplements utilized for MSC manufacturing may lead to clinical complications. Characterization of alternative media formulations is imperative for MSC therapeutic application. Human BMMSC and AdMSC were expanded in media supplemented with either human platelet lysates (HPL), serum-free media/xeno-free FDA-approved culture medium (SFM/XF), or fetal bovine serum (FBS) and the effects on their properties were investigated. The immunophenotype of resting and IFN-γ primed BMMSC and AdMSC remained unaltered in all media. Both HPL and SFM/XF increased the proliferation of BMMSC and AdMSC. Expansion of BMMSC and AdMSC in HPL increased their differentiation, compared to SFM/XF and FBS. Resting BMMSC and AdMSC, expanded in FBS or SFM/XF, demonstrated potent immunosuppressive properties in both non-primed and IFN-γ primed conditions, whereas HPL-expanded MSC exhibited diminished immunosuppressive properties. Finally, IFN-γ primed BMMSC and AdMSC expanded in SFM/XF and HPL expressed attenuated levels of IDO-1 compared to FBS. Herein, we provide strong evidence supporting the use of the FDA-approved SFM/XF medium, in contrast to the HPL medium, for the expansion of MSC towards therapeutic applications

Experimental studies of bubbly flow in a pseudo-2D micro-structured bubble column reactor using digital image analysis

A novel micro-structured bubble column reactor(MSBC) is studied experimentally in this work. Micro-structuring is realized by using a wire mesh inserted in a pseudo-2D bubble column reactor. Different flow configurations of the wire mesh are evaluated by means of visual observation. Three different regimes with bubble cutting, bubble cutting followed by re-coalescence and gas pocket formation was observed for different wire meshes for superficial gas velocities ranging from 5 to 50 mm/s. An advanced Digital Image Analysis technique (DIA) was used to determine the effect of wire mesh on the bubble size distribution, gas holdup and interfacial area. Detailed experiments were performed for wire meshes with mesh openings of 3.3 mm, 3.7 mm and 5.6 mm for superficial gas velocities ranging from 5 to 30 mm/s. Bubble cutting is observed at 3.7 mm mesh opening for superficial gas velocities up to 15 mm/s. At higher superficial gas velocities,the bubble breaks up to very small bubbles and bypasses the wire mesh unaffected

Nonlinear Dynamics of Capacitive Charging and Desalination by Porous Electrodes

The rapid and efficient exchange of ions between porous electrodes and aqueous solutions is important in many applications, such as electrical energy storage by super-capacitors, water desalination and purification by capacitive deionization (or desalination), and capacitive extraction of renewable energy from a salinity difference. Here, we present a unified mean-field theory for capacitive charging and desalination by ideally polarizable porous electrodes (without Faradaic reactions or specific adsorption of ions) in the limit of thin double layers (compared to typical pore dimensions). We illustrate the theory in the case of a dilute, symmetric, binary electrolyte using the Gouy-Chapman-Stern (GCS) model of the double layer, for which simple formulae are available for salt adsorption and capacitive charging of the diffuse part of the double layer. We solve the full GCS mean-field theory numerically for realistic parameters in capacitive deionization, and we derive reduced models for two limiting regimes with different time scales: (i) In the "super-capacitor regime" of small voltages and/or early times where the porous electrode acts like a transmission line, governed by a linear diffusion equation for the electrostatic potential, scaled to the RC time of a single pore. (ii) In the "desalination regime" of large voltages and long times, the porous electrode slowly adsorbs neutral salt, governed by coupled, nonlinear diffusion equations for the pore-averaged potential and salt concentration

Experimental Proof of a Magnetic Coulomb Phase

Spin ice materials are magnetic substances in which the spin directions map onto hydrogen positions in water ice. Recently this analogy has been elevated to an electromagnetic equivalence, indicating that the spin ice state is a Coulomb phase, with magnetic monopole excitations analogous to ice's mobile ionic defects. No Coulomb phase has yet been proved in a real magnetic material, as the key experimental signature is difficult to resolve in most systems. Here we measure the scattering of polarised neutrons from the prototypical spin ice Ho2Ti2O7. This enables us to separate different contributions to the magnetic correlations to clearly demonstrate the existence of an almost perfect Coulomb phase in this material. The temperature dependence of the scattering is consistent with the existence of deconfined magnetic monopoles connected by Dirac strings of divergent length.Comment: 18 pages, 4 fig

Dielectric Resonator Antenna Mounted on Cylindrical Ground Plane for Handheld RFID Reader at 5.8 GHz

Dielectric resonator antenna (DRA) mounted on cylindrical ground plane is investigated for handheld RFID reader applications at 5.8 GHz. The simplicity of the structure makes it practical in terms of cost, space, and ease of fabrication. The radiation characteristics of the antenna in free space and in the presence of a proposed compact reader device model and human hand are calculated. The antenna is circularly polarized and exhibits peak gain of 7.62 dB at 5.8 GHz with high front to back ratio of 15.5 dB. Using the same reader device model, a sequentially feeding 2×2 DRA array mounted on the same cylindrical ground plane is used for RFID reader antenna at 5.8 GHz. The array introduces high gain of 9.36 dB at 5.8 GHz with high front to back ratio of 10.48 dB. The 2×2 DRA array elements exhibit circular polarization over a frequency band of 1.1 GHz. The axial ratio is 1.1 dB at 5.8 GHz. The proposed reader model is simple and has a small size compared with that in the case of planar ground plane. The results are calculated using the finite element method (FEM) and compared with that calculated using the finite integral technique (FIT)