416 research outputs found
Imunoproteção de bovinos contra Anaplasma marginale E Anaplasma centrale, obtidos por cultivo in vitro em células embrionárias de Boophilus microplus.
Imunoproteção de bovinos contra anaplasmose utilizando Anaplasma marginale e Anaplasma centrale obtidos de cultivos in vitro de células embrionárias de Boophilus microplus.
Synthesis of Methyl Carbamates from Primary Aliphatic Amines and Dimethyl Carbonate in Supercritical CO2: Effects of Pressure and Cosolvents and Chemoselectivity
At 130 °C, in the presence of CO2 (5-200 bar), primary aliphatic amines react with dimethyl
carbonate (MeOCO2Me, DMC) to yield methyl carbamates (RNHCO2Me) and N-methylation sideproducts
(RNHMe and RNMe2). The pressure of CO2 largely influences both the reaction conversion
and the selectivity toward urethanes: in general, conversion goes through a maximum (70-80%)
in the midrange (40 bar) and drops at lower and higher pressures, whereas selectivity is continuously
improved (from 50% up to 90%) by an increase of the pressure. This is explained by the multiple
role of CO2 in (i) the acid/base equilibrium with aliphatic amines, (ii) the reactivity/solubility of
RNHCO2
- nucleophiles with/in DMC, and (iii) the inhibition of competitive N-methylation reaction
of the substrates. Cosolvents also affect the reaction: in particular, a drop in selectivity is observed
with polar protic media (i.e., MeOH), plausibly because of solvation effects (through H-bonds) of
RNHCO2
- moieties. The reaction shows also a good chemoselectivity: bifunctional aliphatic amines
bearing either aromatic NH2 or OH substituents [XC6H4(CH2)nNH2, X ) NH2, OH; n ) 1, 2], undergo
methoxycarbonylation reactions exclusively at aliphatic amino groups and give the corresponding
methyl carbamates [XC6H4(CH2)nNHCO2Me] in 39-65% isolated yields
A cultura do arroz de sequeiro no Brasil: problemas e perspectivas.
A produção brasileira de arroz esta concentrada, principalmente, nas regiões Centro-Oeste, Sudeste e Sul. Na região Nordeste destaca-se apenas o Maranhão como grande produtor. No ano agrícola de 77/78, a produção brasileira de arroz foi cerca de 7,5 milhões de toneladas, obtidas em aproximadamente 5,6 milhões de hectares, com a produtividade média de 1.340 kg por hectare. Desta produção, aproximadamente 91,2% ou 6.842.000 toneladas foram produzidas nos estados do Rio Grande do Sul, Mato Grosso do Sul, Maranhão, Mato Grosso, Minas Gerais, Goiás, Santa Catarina, São Paulo e Paraná. No Rio Grande do Sul e Santa Catarina, predomina o sistema de cultivo de arroz com irrigação por inundação, enquanto que nos Estados de Mato Grosso, Mato Grosso do Sul, Maranhão, Minas Gerais, Goiás, São Paulo e Paraná, o sistema predominante é o de sequeiro
Hairy garlic (Allium subhirsutum) from Sicily (Italy): LC-DAD-MSn analysis of secondary metabolites and in vitro biological properties
Allium subhirsutum, known as hairy garlic, is a bulbous plant widespread in the Mediterranean area and locally used as a food and spice. In the present study, the chemical profile of the ethanolic extracts from bulbs (BE) and aerial parts (APE) were analyzed by HPLC-ESI-MSn, and antioxidant properties were evaluated by DPPH, ABTS and TEAC assays. The traditional use in the diet, and the well documented biological activity of Allium species suggest a potential as a new nutraceutical. For this reason, the potential usefulness of this food can be considered in the treatment and prevention of degenerative Alzheimer disease. For this reason, acetylcholinesterase inhibitory property was investigated. Furthermore, due to the observed presence of sulfur-containing and phenolic constituents, the cytotoxicity on tumor cells line was investigated. Results revealed significant AChE inhibitory activity for BE and APE. Both extracts exhibited also moderate antioxidant properties in the in vitro assays. Finally, limited cytotoxic activity was observed towards Human colon carcinoma and adenocarcinoma cell line, with differences between the individual parts tested. HPLC-ESI-MSn analysis showed that hairy garlic is a good source of sulphur compounds, flavonoids and phenylpropanoids derivatives, thus being a valid alternative to the common garlic (A. sativum). This work opens new opportunities for the application of A. subhirsutum as a health-promoting food
Adaptive research, preproduction testing, and production programs in Brazil.
The Brazilian agricultural research system was developed under the influence of the diffusion model imported from the more developed countries (5). The model implies a freedom of choice in research projects so that scarce resources can be allocated to a broad range of research topics depending on the investigator's decision. The tendency to develop research activities oriented toward solving real problems was supposed to result from pressure by farmers on government research agencies. Extension services were seen as the means of bridging the gap between research agencies and farmer fields
Phytochemical fingerprinting and in vitro bioassays of the ethnomedicinal fern tectaria coadunata (J. Smith) C. Christensen from Central Nepal
Tectaria coadunata, an ethnomedicinal fern used in Nepal to treat a large number of diseases, has been poorly studied with regard to its phytochemical composition and possible bioactivity. This study was performed with the aim of supporting traditional medicine as a new source of bioactive constituents. Phytochemical compositions of methanol extracts were determined by nuclear magnetic resonance (NMR), liquid chromatography-diode array detector-mass spectrophotometry (LC-DAD-MS), and liquid chromatography-fluorescence-mass spectrometry. Quali-quantitative data revealed large amount of procyanidins, mainly of the A-type, as well as eriodictyol-7-O-glucuronide and luteolin-7-O-glucoronide as main constituents. The antioxidant, cytotoxic, and inhibitory activity of five enzymes that are implicated in human diseases was evaluated for the extract and fractions. High free-radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays and inhibitory activities against cholinesterases and tyrosinase were observed. Furthermore, a moderate cytotoxic effect was observed on the 2008 and BxPC3 cell lines. Overall results showed potential usefulness of this fern as a source of phytochemicals for pharmaceutical uses
In Vitro and in Vivo Effectiveness of Carvacrol, Thymol and Linalool against Leishmania infantum
Background: One of the most important causative agents of visceral leishmaniasis (VL) is Leishmania infantum, which is mainly spread by Phlebotomus and Lutzomyia sandflies in the Old and New World, respectively. Novel and effective drugs to manage this neglected vector-borne disease are urgently required. In this study, we evaluated the toxicity of carvacrol, thymol and linalool, three common essential oil constituents, on amastigotes and promastigotes of L. infantum. Methods: in vitro experiments were performed by 24 h MTT assay. Carvacrol, thymol and linalool at concentrations ranging from 1.3 to 10 µg/mL were tested on promastigotes of L. infantum. For in vivo test, two groups of hamsters (Mesocricetus auratus) received 100 mg/kg of body weight/day of carvacrol and thymol as intraperitoneal injection on day 7 post-infection, followed by a 48 h later injection. The third group was treated with the glucantime as standard drug (500 mg/kg) and the last group (control) just received normal saline. On the 16th day, the number of parasites and histopathological changes in liver and spleen were investigated. Results: 24 h MTT assay showed promising antileishmanial activity of thymol and carvacrol, with IC50 values of 7.2 (48 µM) and 9.8 µg/mL (65 µM), respectively. Linalool at all concentrations did not affect L. infantum promastigote viability. In vivo toxicity data of carvacrol and thymol showed that the former at 100 mg/kg was the safest and most effective treatment with little side effects on the liver. Conclusions: Overall, thymol and carvacrol are highly promising candidates for the development of effective and safe drugs in the fight against VL
CDKN1B mutation and copy number variation are associated with tumor aggressiveness in luminal breast cancer
The CDKN1B gene, encoding for the CDK inhibitor p27kip1, is mutated in defined human cancer subtypes, including breast, prostate carcinomas and small intestine neuroendocrine tumors. Lessons learned from small intestine neuroendocrine tumors suggest that CDKN1B mutations could be subclonal, raising the question of whether a deeper sequencing approach could lead to the identification of higher numbers of patients with mutations. Here, we addressed this question and analyzed human cancer biopsies from breast (n = 396), ovarian (n = 110) and head and neck squamous carcinoma (n = 202) patients, using an ultra-deep sequencing approach. Notwithstanding this effort, the mutation rate of CDKN1B remained substantially aligned with values from the literature, showing that essentially only hormone receptor-positive breast cancer displayed CDKN1B mutations in a relevant number of cases (3%). However, the analysis of copy number variation showed that another fraction of luminal breast cancer displayed loss (8%) or gain (6%) of the CDKN1B gene, further reinforcing the idea that the function of p27kip1 is important in this type of tumor. Intriguingly, an enrichment for CDKN1B alterations was found in samples from premenopausal luminal breast cancer patients (n = 227, 4%) and in circulating cell-free DNA from metastatic luminal breast cancer patients (n = 59, 8.5%), suggesting that CDKN1B alterations could correlate with tumor aggressiveness and/or occur later during disease progression. Notably, many of the identified somatic mutations resulted in p27kip1 protein truncation, leading to loss of most of the protein or of its C-terminal domain. Using a gene-editing approach in a luminal breast cancer cell line, MCF-7, we observed that the expression of p27kip1 truncating mutants that lose the C-terminal domains failed to rescue most of the phenotypes induced by CDKN1B gene knockout, indicating that the functions retained by the C-terminal portion are critical for its role as an oncosuppressor, at least in luminal breast cancer. © 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland
Progressive dry-core-wet-rim hydration trend in a nested-ring topology of protein binding interfaces
<p>Abstract</p> <p>Background</p> <p>Water is an integral part of protein complexes. It shapes protein binding sites by filling cavities and it bridges local contacts by hydrogen bonds. However, water molecules are usually not included in protein interface models in the past, and few distribution profiles of water molecules in protein binding interfaces are known.</p> <p>Results</p> <p>In this work, we use a tripartite protein-water-protein interface model and a nested-ring atom re-organization method to detect hydration trends and patterns from an interface data set which involves immobilized interfacial water molecules. This data set consists of 206 obligate interfaces, 160 non-obligate interfaces, and 522 crystal packing contacts. The two types of biological interfaces are found to be drier than the crystal packing interfaces in our data, agreeable to a hydration pattern reported earlier although the previous definition of immobilized water is pure distance-based. The biological interfaces in our data set are also found to be subject to stronger water exclusion in their formation. To study the overall hydration trend in protein binding interfaces, atoms at the same burial level in each tripartite protein-water-protein interface are organized into a ring. The rings of an interface are then ordered with the core atoms placed at the middle of the structure to form a nested-ring topology. We find that water molecules on the rings of an interface are generally configured in a dry-core-wet-rim pattern with a progressive level-wise solvation towards to the rim of the interface. This solvation trend becomes even sharper when counterexamples are separated.</p> <p>Conclusions</p> <p>Immobilized water molecules are regularly organized in protein binding interfaces and they should be carefully considered in the studies of protein hydration mechanisms.</p
- …