5 research outputs found
Freqüência de anticorpos anti-Erhlichia canis, Borrelia burgdorferi e antígenos de Dirofilaria immitis em cães na microrregião Ilhéus-Itabuna, Bahia, Brasil
Avaliação da coagulação plasmática e plaquetometria em cães não infectados e infectados experimentalmente com Ehrlichia spp.
Molecular Detection Of Cytauxzoon Spp. In Asymptomatic Brazilian Wild Captive Felids
Cytauxzoon spp. DNA was detected for the first time in Mood samples from asymptomatic Brazilian wild captive felids. In 2006, 72 EDTA blood samples from seven wild felids species: Puma concolor (puma), Leopardus pardalis (ocelot), Puma yagouaroundi (jaguarundi), Leopardus wiedii (margay), Leopardus tigrinus (little spotted cat), Oncifelis colocolo (pampas cat) and Panthera onca (jaguar) were analyzed using polymerase chain reaction to amplify the 18S rRNA gene segment in order to verify the presence of Cytauxzoon spp. DNA. Nine samples were positive: six ocelots, two pumas, and one jaguar. In Brazil, wild felids may be natural reservoirs for Cutauxzoon spp. © 2009 Wildlife Disease Association.451234237Birkenheuer, A.J., Marr, H., Alleman, A.R., Levy, M.G., Breitschwerdt, E.B., Development and evaluation of a PCR assay for the detection of Cytauxzoon felis DNA in feline blood samples (2006) Veterinary Parasitology, 137, pp. 144-149Blouin, E.F., Kocan, A.A., Glenn, B.L., Kocan, K.M., Transmission of cytauxzoon felis Kier, 1979 from bobcats, Felis rufus (Schreber), to domestic cats by dermacentor variabilis (Say 1821) (1984) Journal of Wildlife Diseases, 20, pp. 241-242Kocan, K.M., Evidence of a limited schizogonous cycle for cytauxzoon felis in bobcats following exposure to infected ticks (1987) Journal of Wildlife Diseases, 23, pp. 499-501Butt, M.T., Bowman, D., Bahr, M.C., Roelke, M.E., Iatrogenic transmission of cytauxzoon felis from a Florida panther (Felis concolor coryi) to a domestic cat (1991) Journal of Wildlife Diseases, 27, pp. 342-347Garner, M.M., Lung, M.P., Citing, S., Greiner, E.C., Harvey, J.W., Homer, B.L., Fatal cytauxzoonosis in a captive-reared white tiger (Panthera tigris) (1996) Veterinary Pathology, 33, pp. 82-86Glenn, B.L., Rolley, R.E., Kocan, A.A., Cytauxzoon-like piroplasms in erythrocytes of wild-trapped bobcats in Oklahoma (1982) Journal of American Veterinary Medical Association, 181, pp. 1251-1253Greene, C.E., Meinkotii, J., Kocan, A.A., Cytauxzoonosis (2006) Infectious Diseases of Dog, Cat, pp. 722-733. , 3rd Edition, C. E. Greene (ed.). Saunders Elsevier, St. Louis, MissouriHaber, M.D., Tucker, M.D., Mark, H.S., Lew, J.K., Burgess, J., Lappin, M.R., Birkenheuer, A.J., The detection of Cytauxzoon felis in apparently healthy free-roaming cats in the USA (2007) Veterinary Parasitology, 146, pp. 316-320Hoskins, J.D., Canine haemobartonellosis, canine hepatozoonosis and feline cytauxzoonosis (1991) Veterinary Clinics of North America: Small Animal Practice, 21, pp. 129-140Joyner, P.H., Reichard, M.V., Meinkoth, J.H., Milne, V.E., Confer, A.W., Kocan, A.A., Hoover, J.P., Experimental infection of domestic cats (Felis domesticus) with Cytauxzoon manul from Pallas' cats (Otocohbus manul) (2007) Veterinary Parasitology, 146, pp. 302-306Ketz-Riley, C.J., Reichard, M.V., Van Den Bussche, R.A., Hoover, J.P., Meinkoth, J., Kocan, A.A., An intraerythrocytic small piroplasm in wild-caught Pallas' cats (Otocolobus manul) from Mongolia (2003) Journal of Wildlife Diseases, 39, pp. 424-430Kocan, A.A., Blouin, E.F., Hematologic and serum chemical values for free-ranging bobcats, Felis rufus (Schreber) with reference to animals with, natural infections of Cytauxzoon felis Kier, 1979 (1985) Journal of Wildlife Diseases, 21, pp. 190-192Labruna, M.B., De Paula, C.D., Lima, T.F., Sana, D.A., Ticks (Acari: Ixodidae) on wild animals from the port-primavera hydroeletric power station area, Brazil (2002) Memórias Do Instituto Oswaldo Cruz, 97, pp. 1113-1136Luaces, I., Aguirre, E., Garcia-Montijano, M., Velarde, J., Tesouro, M.A., Sánchez, C., Galka, M., Sainz, A., First report of an intra-erythrocytic small piroplasm in wild Iberian lynx (Lynx pardinus) (2005) Journal of Wildlife Diseases, 41, pp. 810-815Meinkoth, J., Kocan, A.A., Whitworth, L., Murphy, G., Fox, J.C., Woods, J.P., Cats surviving natural infection with cytauxzoon fells: 18 cases (1997-1998) (2000) Journal ' of Veterinary Internal Medicine, 14, pp. 521-525Mendes-De-Almeida, F., Labarthe, N., Guerrero, J., Faria, M.C.F., Branco, A.S., Pereira, C.D., Barreira, J.D., Pereira, M.J.S., Followup of the health conditions of an urban colony of free-roaming cats (Felis catus Linnaeus, 1758) in. the city of Rio de Janeiro (2007) Veterinary Parasitology, 147, pp. 209-211Millán, J., Naranjo, V., Rodríguez, A., La Lastra, J.M.P., Mangold, A.J., Fuente, J.L.A., Prevalence of infection and 18S rRNA gene sequences of cytauxzoon species in Iberian lynx (Lynx pardinus) in Spain (2007) Parasitology, 134, pp. 995-1001Nietfield, J.C., Pollock, C., Fatal cytauxzoonosis in a free-ranging bobcat (Lynx rufus) (2002) Journal of Wildlife Diseases, 38, pp. 607-610Peixoto, P.V., Soares, C.O., Scofield, A., Santiago, C.D., Frana, T.N., Barros, S.S., Fatal cytauxzoonosis in captive-reared lions in Brazil (2007) Veterinary Parasitology, 145, pp. 383-387Reichard, M.V., Van Den Bussche, R.A., Meinkoth, J., Hoover, J.P., Kocan, A.A., A new species of cytauxzoon from pallas' cats caught in Mongolia and comments on the systematics and taxonomy of piroplasmids (2005) Journal of Parasitology, 91, pp. 420-426Rotstein, D.S., Taylor, S.K., Harvey, J.W., Bean, J., Haematologic effects of cytauxzoonosis in Florida panthers and Texas cougars in Florida (1999) Journal of Wildlife Diseases, 35, pp. 613-61
Canine ehrlichiosis: clinical, hematological, serological and molecular aspects Erliquiose canina: aspectos clínicos, hematológicos, sorológicos e moleculares
The aim of the present study was to compare the direct detection methods of Ehrlichia canis (blood smears and nested PCR), serological tests (Dot-ELISA and Immunofluorescent Antibody Test - IFAT), and demonstrate the most suitable test for the diagnosis of different stages of infection. Blood samples and clinical data were collected from 30 dogs examined at the Veterinary Teaching Hospital, UNESP, Jaboticabal, SP, Brazil. The clinical signs most frequently observed were apathy, anorexia, pale mucous membrane, fever, lymphadenopathy, splenomegaly, hemorrhages and uveitis. Evaluating the humoral immune response, 63.3% of the sera were IFAT positive, while 70% were Dot-ELISA positive. By nestedPCR 53.3% of the samples were positive. Comparing these techniques it was concluded that serology and nPCR are the most suitable tests to confirm the diagnosis of canine ehrlichiosis, however it should be always treated as a complementary data to clinical and hematological evaluation. Serology has an important role in the subclinical and in the chronic phase, nPCR is recommended in the acute stage, and, especially, to identify the ehrlichia specie.<br>O objetivo deste estudo foi comparar técnicas para detecção direta de Ehrlichia canis (detecção de mórulas em esfregaço sangüíneo e nested PCR), testes sorológicos (Dot-ELISA e Reação de Imunofluorescência Indireta - RIFI) e identificar o teste mais adequado para o diagnóstico de diferentes fases da infecção. Amostras sangüíneas e dados dos prontuários clínicos foram colhidos de 30 cães examinados no Hospital Veterinário, UNESP - Jaboticabal, SP. Os sinais clíncos mais freqüentemente observados foram apatia, inapetência, palidez de mucosas, febre, linfadenopatia, esplenomegalia, hemorragias e uveíte. Na avaliação da resposta imune humoral, observou-se que 63,3% das amostras foram positivas na RIFI, e 70% no Dot-ELISA. Na nPCR, foram detectadas 53,3% de amostras positivas. Ao comparar estas técnicas, concluiu-se que a sorologia e a nPCR são testes adequados para a confirmação do diagnóstico da erliquiose canina. Entretanto, os resultados destas técnicas devem sempre ser complementares ao exame clínico e hematológico. A sorologia tem um importante papel nas fases subclínica e crônica da doença, por isso recomenda-se a nPCR para o diagnóstico na fase aguda e, especialmente, para a identificação da espécie de erliquia envolvida