Uneven ground? Intersectional gender inequalities in the commercialized cassava seed system in Tanzania

Open Access JournalCassava (Manihot esculenta Crantz) is an important crop in Africa, especially to women who rely on it as a household staple food and source of income. In Tanzania, a recent move toward commercializing the cassava seed system resulted in significantly fewer women than men farmers, known as Cassava Seed Entrepreneurs (CSEs), producing improved seed for sale to fellow farmers. To document the barriers and constraints that create gender inequalities in the seed system to better understand women’s low representation and experiences in commercialized cassava seed production, we carried out a mixed-methods study in the Southern, Eastern, and Lake Zones of Tanzania in 2021. The quantitative analysis found differences in key individual and household characteristics between CSEs and farmers who aspired to be but did not participate as CSEs (or A-CSEs) as well as between women CSEs and women A-CSEs. After running a logistic regression, results indicated that sex of the farmer (being male) was a statistically significant predictor of participating as a CSE (p < 0.05), along with having a secondary education (p < 0.05) and owning a bank account (p < 0.01). The qualitative analysis highlighted challenges women CSEs face. They spoke about having lower access to and control over prerequisite resources, which are shaped by other intersecting social identities such as marital status and age. Gender stereotypes about their capacities to manage their seed businesses can demotivate them from carrying out their work as well as experiences dealing with discriminatory gender norms that limit their travel to attend trainings outside their communities. Despite these barriers, some women CSEs expressed positive outcomes that have accrued from their participation in commercialized seed production, including enhanced social status and improved living standards. For the commercialized cassava seed system to be more socially inclusive and sustainable, we argue that there is need to adopt gender-aware approaches to address the underlying barriers and biases that exclude women and other social groups. Development efforts should consider combining social change innovations with seed system interventions to address the inequitable norms and power relations that create unique constraints for women to operate effectively as seed entrepreneurs

Laboratory adapted Escherichia coli K-12 becomes a pathogen of Caenorhabditis elegans upon restoration of O antigen biosynthesis

Escherichia coli has been the leading model organism for many decades. It is a fundamental player in modern biology, facilitating the molecular biology revolution of the last century. The acceptance of E.?coli as model organism is predicated primarily on the study of one E. coli lineage; E. coli K-12. However, the antecedents of today's laboratory strains have undergone extensive mutagenesis to create genetically tractable offspring but which resulted in loss of several genetic traits such as O antigen expression. Here we have repaired the wbbL locus, restoring the ability of E. coli K-12 strain MG1655 to express the O antigen. We demonstrate that O antigen production results in drastic alterations of many phenotypes and the density of the O antigen is critical for the observed phenotypes. Importantly, O antigen production enables laboratory strains of E. coli to enter the gut of the Caenorhabditis elegans worm and to kill C. elegans at rates similar to pathogenic bacterial species. We demonstrate C. elegans killing is a feature of other commensal E.?coli. We show killing is associated with bacterial resistance to mechanical shear and persistence in the C. elegans gut. These results suggest C. elegans is not an effective model of human-pathogenic E. coli infectious disease