51 research outputs found

    Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

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    Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy

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    Impact of Si substrate resistivity on the non-linear behaviour of RF CPW transmission lines

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    Non-linear behaviour of RF coplanar transmission lines is analyzed for various values of Si substrate resistivitiy. Based on small-signal measurements performed under different DC bias conditions, voltage dependent capacitance and conductance per unit length of the transmission line are extracted and compared for several silicon substrates. Harmonic distortion of large RF signal at 900 MHz along CPW lines is measured using a spectrum analyzer based setup as well as with a LSNA which gives us access to the phase of the harmonic components. For an input power of +25 dBm, the highest harmonic component (2/sup nd/) is as high as -15, -57, -37 and -63 dBm for resistivity substrates of 20, 500, 5 k and 2 k Omega -cm, respectively. A reduction of 45 and 15 dB for all harmonic components was obtained for the 5 and 2 k Omega -cm HR-Si substrates, respectively, when a trap-rich passivation layer was used at the Si/SiO/sub 2/ interface, and for both characterization setups. The impact of the resistivity value on signal distortion with its relation to the bias-dependence substrate characteristic and the efficiency of the trap mechanism of the passivation layer are for the first time introduced from experimental result considerations.Anglai
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