Fluctuations in Ca, Mg and P levels in the hemolymph, muscle, midgut gland and exoskeleton during the moult cycle of the Indian white prawn, Penaeus indicus (Decapoda: Penaeidae)

Fluctuations of Ca, Mg and P in the hemolymph, midgut gland, muscle and exoskeleton of the penaeid prawn Penaeus indicus during different stages of the moulting cycle have been investigated. 2. Haemolymph, midgut gland and muscle showed a high content of Ca during late premoult stages and low content in late postmoult and intermoult stages. In exoskeletal tissue the Ca level was high in intermoult and early premoult stages and the lowest level was recorded in the early postmoult stage. Magnesium showed an almost similar trend to that of Ca. Phosphorus content did not show noticeable changes in haemolymph and muscle during moult cycle; in exoskeleton, higher levels were recorded in last premoult and early postmoult stages. 3. The quantitative distribution of Ca, Mg and P in different parts of the exoskeleton was mapped

Au9+ swift heavy ion irradiation of Zn[CS(NH2)2]3SO4 crystal: Crystalline perfection and optical properties

The single crystal of tris(thiourea)zinc sulphate (Zn[CS(NH2)2]3SO4) was irradiated by 150 MeV Au9+ swift heavy ions and analyzed in comparison with pure crystal for crystalline perfection and optical properties. The Fourier transform infrared and x-ray powder diffraction inferred that swift ions lead the disordering and breaking of molecular bonds in lattice without formation of new structural phases. High resolution X-ray diffraction (HRXRD) revealed the abundance of point defects, and formation of mosaics and low angle grain boundaries in the irradiated region of crystal. The swift ion irradiation found to affect the lattice vibrational modes and functional groups significantly. The defects induced by heavy ions act as the color centers and resulted in enhance of photoluminescence emission intensity. The optical transparency and band gap found to be decreased.Comment: 7 page

Experimental Studies on Abrasive Water Jet Cutting of Nano SiC Particles Filled Hybrid Basalt-Glass Fibre-Reinforced Epoxy Composites

Abrasive water jet machining (AWJM) is extensively beneficial in machining materials that are hard to cut. This investigation deals with AWJM of Nano SiC filled Epoxy reinforced with basalt-glass fiber hybrid composite. The composite is prepared by compression moulding technique. Experimental trails are performed to evaluate the impact of every process parameter on the responses i.e., surface roughness (Ra) and Material Removal Rate (MRR). The experiments are conducted by changing the standoff distance (SD), traverse speed (TS) and water pressure. The performance of the conducted experiment is analysed using a Swarm intelligence algorithm. Surface roughness and MRR are maximized by using the combination of optimum process parameter levels of 9.72 mm/min speed, 5.78 mm stand-off distance and 553 MPa jet pressure. Scanning Electron Microscopic (SEM) images are employed in detecting the morphology of machined surface and confirmed the presence of voids and fibre pull-out

Robust Textural Features for Real Time Face Recognition

Automatic face recognition in real life environment is challenged by various issues such as the object motion, lighting conditions, poses and expressions. In this paper, we present the development of a system based on a refined Enhanced Local Binary Pattern (ELBP) feature set and a Support Vector Machine (SVM) classifier to perform face recognition in a real life environment. Instead of counting the number of 1\u27s in ELBP, we use the 8-bit code of the thresholded data as per the ELBP rule, and then binarize the image with a predefined threshold value, removing the small connections on the binarized image. The proposed system is currently trained with several people\u27s face images obtained from video sequences captured by a surveillance camera. One test set contains the disjoint images of the trained people\u27s faces to test the accuracy and the second test set contains the images of non-trained people\u27s faces to test the percentage of the false positives. The recognition rate among 570 images of 9 trained faces is around 94%, and the false positive rate with 2600 images of 34 non-trained faces is around 1%. Research work is progressing for the recognition of partially occluded faces as well. An appropriate weighting strategy will be applied to the different parts of the face area to achieve a better performance

Studies on moult staging, moulting duration and moulting behaviour in Indian White shrimp Penaeus indicus Milne Edwards (Decapoda: Penaeidae)

Characterisation and classification of complete moult cycle of Penaeus indicus have been worked out on the basis of setal development Seotogenic moults taging was found to be a rapid and simple technique. Since excision of appendage is not required, this technique is non-destuctive and permits repetitive moult staging of an individual shrimp

Multiplicity of carbohydrate-binding sites in β-prism fold lectins: occurrence and possible evolutionary implications

The β-prism II fold lectins of known structure, all from monocots, invariably have three carbohydrate-binding sites in each subunit/domain. Until recently, β-prism I fold lectins of known structure were all from dicots and they exhibited one carbohydrate-binding site per subunit/domain. However, the recently determined structure of the β-prism fold I lectin from banana, a monocot, has two very similar carbohydrate-binding sites. This prompted a detailed analysis of all the sequences appropriate for two-lectin folds and which carry one or more relevant carbohydrate-binding motifs. The very recent observation of a β-prism I fold lectin, griffithsin, with three binding sites in each domain further confirmed the need for such an analysis. The analysis demonstrates substantial diversity in the number of binding sites unrelated to the taxonomical position of the plant source. However, the number of binding sites and the symmetry within the sequence exhibit reasonable correlation. The distribution of the two families of β-prism fold lectins among plants and the number of binding sites in them, appear to suggest that both of them arose through successive gene duplication, fusion and divergent evolution of the same primitive carbohydrate-binding motif involving a Greek key. Analysis with sequences in individual Greek keys as independent units lends further support to this conclusion. It would seem that the preponderance of three carbohydrate-binding sites per domain in monocot lectins, particularly those with the β-prism II fold, is related to the role of plant lectins in defence

Structural transformations in protein crystals caused by controlled dehydration

Recent experiments in this laboratory on structural transformations caused by controlled dehydration of protein crystals have been reviewed. X-ray diffraction patterns of the following crystals have been examined under varying conditions of environmental humidity in the relative humidity range of 100-75%: a new crystal form of bovine pancreatic ribonuclease A grown from acetone solution in tris buffer (I), the well-known monoclinic form of the protein grown from aqueous ethanol (II), the same form grown from a solution of 2-methyl pentan-2,4-diol in phosphate buffer (III), tetragonal (IV), orthorhombic (V), monoclinic (VI) and triclinic (VII) hen egg white lysozyme, porcine 2 Zn insulin (VIII), porcine 4 Zn insulin (IX) and the crystals of concanavalin A(X). I, II, IV, V and VI undergo one or more transformations as evidenced by discontinuous changes in the unit cell dimensions, the diffraction pattern and the solvent content. Such water-mediated transformations do not appear to occur in the remaining crystals in the relative humidity range explored. The relative humidity at which the transformation occurs is reduced when 2-methyl pentan-2,4-diol is present in the mother liquor. The transformations are affected by the crystal structure but not by the amount of solvent in the crystals. The X-ray investigations reviewed here and other related investigations emphasize the probable importance of water-mediated transformations in exploring hydration of proteins and conformational transitions in them