Implementation of a geometrically informed and energetically constrained mesoscale eddy parameterization in an ocean circulation model

The global stratification and circulation of the ocean and their sensitivities to changes in forcing depend crucially on the representation of the mesoscale eddy field. Here, a geometrically informed and energetically constrained parameterization framework for mesoscale eddies --- termed GEOMETRIC --- is proposed and implemented in three-dimensional primitive equation channel and sector models. The GEOMETRIC framework closes mesoscale eddy fluxes according to the standard Gent--McWilliams scheme, but with the eddy transfer coefficient constrained by the depth-integrated eddy energy field, provided through a prognostic eddy energy budget evolving with the mean state. It is found that coarse resolution calculations employing GEOMETRIC broadly reproduce model sensitivities of the eddy permitting reference calculations in the emergent circumpolar transport, meridional overturning circulation profile and the depth-integrated eddy energy signature; in particular, eddy saturation emerges in the sector configuration. Some differences arise, attributed here to the simple prognostic eddy energy budget employed, to be improved upon in future investigations. The GEOMETRIC framework thus proposes a shift in paradigm, from a focus on how to close for eddy fluxes, to focusing on the representation of eddy energetics.Comment: 19 pages, 9 figures, submitted to Journal of Physical Oceanography; comments welcome. (Copyright statement: see section 7a of https://www.ametsoc.org/ams/index.cfm/publications/ethical-guidelines-and-ams-policies/ams-copyright-policy/

Morpheus: Advancing Technologies for Human Exploration

NASA's Morpheus Project has developed and tested a prototype planetary lander capable of vertical takeoff and landing. Designed to serve as a vertical testbed (VTB) for advanced spacecraft technologies, the vehicle provides a platform for bringing technologies from the laboratory into an integrated flight system at relatively low cost. This allows individual technologies to mature into capabilities that can be incorporated into human exploration missions. The Morpheus vehicle is propelled by a LOX/Methane engine and sized to carry a payload of 1100 lb to the lunar surface. In addition to VTB vehicles, the Project s major elements include ground support systems and an operations facility. Initial testing will demonstrate technologies used to perform autonomous hazard avoidance and precision landing on a lunar or other planetary surface. The Morpheus vehicle successfully performed a set of integrated vehicle test flights including hot-fire and tethered hover tests, leading up to un-tethered free-flights. The initial phase of this development and testing campaign is being conducted on-site at the Johnson Space Center (JSC), with the first fully integrated vehicle firing its engine less than one year after project initiation. Designed, developed, manufactured and operated in-house by engineers at JSC, the Morpheus Project represents an unprecedented departure from recent NASA programs that traditionally require longer, more expensive development lifecycles and testing at remote, dedicated testing facilities. Morpheus testing includes three major types of integrated tests. A hot-fire (HF) is a static vehicle test of the LOX/Methane propulsion system. Tether tests (TT) have the vehicle suspended above the ground using a crane, which allows testing of the propulsion and integrated Guidance, Navigation, and Control (GN&C) in hovering flight without the risk of a vehicle departure or crash. Morpheus free-flights (FF) test the complete Morpheus system without the additional safeguards provided during tether. A variety of free-flight trajectories are planned to incrementally build up to a fully functional Morpheus lander capable of flying planetary landing trajectories. In FY12, these tests will culminate with autonomous flights simulating a 1 km lunar approach trajectory, hazard avoidance maneuvers and precision landing in a prepared hazard field at the Kennedy Space Center (KSC). This paper describes Morpheus integrated testing campaign, infrastructure, and facilities, and the payloads being incorporated on the vehicle. The Project s fast pace, rapid prototyping, frequent testing, and lessons learned depart from traditional engineering development at JSC. The Morpheus team employs lean, agile development with a guiding belief that technologies offer promise, but capabilities offer solutions, achievable without astronomical costs and timelines

Project Morpheus: Lessons Learned in Lander Technology Development

NASA's Morpheus Project has developed and tested a prototype planetary lander capable of vertical takeoff and landing, that is designed to serve as a testbed for advanced spacecraft technologies. The lander vehicle, propelled by a LOX/Methane engine and sized to carry a 500kg payload to the lunar surface, provides a platform for bringing technologies from the laboratory into an integrated flight system at relatively low cost. Designed, developed, manufactured and operated in-house by engineers at Johnson Space Center, the initial flight test campaign began on-site at JSC less than one year after project start. After two years of testing, including two major upgrade periods, and recovery from a test crash that caused the loss of a vehicle, flight testing will evolve to executing autonomous flights simulating a 500m lunar approach trajectory, hazard avoidance maneuvers, and precision landing, incorporating the Autonomous Landing and Hazard Avoidance (ALHAT) sensor suite. These free-flights are conducted at a simulated planetary landscape built at Kennedy Space Center's Shuttle Landing Facility. The Morpheus Project represents a departure from recent NASA programs and projects that traditionally require longer development lifecycles and testing at remote, dedicated testing facilities. This paper expands on the project perspective that technologies offer promise, but capabilities offer solutions. It documents the integrated testing campaign, the infrastructure and testing facilities, and the technologies being evaluated in this testbed. The paper also describes the fast pace of the project, rapid prototyping, frequent testing, and lessons learned during this departure from the traditional engineering development process at NASA's Johnson Space Center

Effect of ocean acidification on otolith development in larvae of a tropical marine fish

© The Author(s), 2011. This article is distributed under the terms of the Creative Commons Attribution 3.0 License. The definitive version was published in Biogeosciences 8 (2011): 1631-1641, doi:10.5194/bg-8-1631-2011.Calcification in many invertebrate species is predicted to decline due to ocean acidification. The potential effects of elevated CO2 and reduced carbonate saturation state on other species, such as fish, are less well understood. Fish otoliths (earbones) are composed of aragonite, and thus, might be susceptible to either the reduced availability of carbonate ions in seawater at low pH, or to changes in extracellular concentrations of bicarbonate and carbonate ions caused by acid-base regulation in fish exposed to high pCO2. We reared larvae of the clownfish Amphiprion percula from hatching to settlement at three pHNBS and pCO2 levels (control: ~pH 8.15 and 404 μatm CO2; intermediate: pH 7.8 and 1050 μatm CO2; extreme: pH 7.6 and 1721 μatm CO2) to test the possible effects of ocean acidification on otolith development. There was no effect of the intermediate treatment (pH 7.8 and 1050 μatm CO2) on otolith size, shape, symmetry between left and right otoliths, or otolith elemental chemistry, compared with controls. However, in the more extreme treatment (pH 7.6 and 1721 μatm CO2) otolith area and maximum length were larger than controls, although no other traits were significantly affected. Our results support the hypothesis that pH regulation in the otolith endolymph can lead to increased precipitation of CaCO3 in otoliths of larval fish exposed to elevated CO2, as proposed by an earlier study, however, our results also show that sensitivity varies considerably among species. Importantly, our results suggest that otolith development in clownfishes is robust to even the more pessimistic changes in ocean chemistry predicted to occur by 2100

The importance of 12R-lipoxygenase and transglutaminase activities in the hydration-dependent ex vivo maturation of corneocyte envelopes

Background Terminally differentiated keratinocytes acquire corneocyte protein envelopes (CPE) complexed with corneocyte lipid envelopes (CLE). These two structural components of the corneocyte envelopes (CE) undergo maturation by gaining in hydrophobicity, rigidity and surface area. Linoleoyl acylceramides are processed by 12R‐lipoxygenase (12R‐LOX) and other enzymes before transglutaminase (TG) attaches ω‐hydroxyceramides to involucrin in the CPE. Concurrently, structural proteins are cross‐linked by TG that has been activated by cathepsin D (CathD). Objectives The primary aim of this work was to demonstrate the impact of relative humidity (RH) during ex vivo CE maturation. Low, optimal and high RH were selected to investigate the effect of protease inhibitors (PI) on CE maturation and TG activity; in addition, 12R‐LOX and CathD activity were measured at optimal RH. Finally, the effect of glycerol on ex vivo CE maturation was tested at low, optimal and high RH. Methods The first and ninth tape strip of photo‐exposed (PE) cheek and photo‐protected (PP) post auricular sites of healthy volunteers were selected. Ex vivo CE maturation was assessed via the Relative CE Maturity (RCEM) approach based on CE rigidity and hydrophobicity. The second and eighth tapes were exposed to RH in presence of inhibitors. Results Irrespective of tape stripping depth, CEs from PE samples attained CE rigidity to the same extent as mature CEs from the PP site, but such improvement was lacking for CE hydrophobicity. 70% RH was optimal for ex vivo CE maturation. The inhibition of 12R‐LOX activity resulted in enhanced CE rigidity which was reduced by the TG inhibitor. CE hydrophobicity remained unchanged during ex vivo maturation in the presence of TG or 12R‐LOX inhibition. CE hydrophobicity was enhanced in the presence of glycerol at 44% RH and 100% RH but not at 70% RH. Furthermore, TG activity was significantly diminished at 100% Rh compared to the commercial inhibitor LDN‐27219. However, a protease inhibitor mix reversed the negative effect of over hydration. Conclusion The study adds to the understanding of the roles of 12R‐LOX and TG activity in CE maturation, and gives further insight into the effect of glycerol on the SC

Turbulent drag on a low-frequency vibrating grid in superfluid He-4 at very low temperatures

We present measurements of the dissipative turbulent drag on a vibrating grid in superfluid He-4 over a wide range of (low) frequencies. At high velocities, the dissipative drag is independent of frequency and is approximately the same as that measured in normal liquid He-4. We present measurements on a similar grid in superfluid He-3-B at low temperatures which shows an almost identical turbulent drag coefficient at low frequencies. However, the turbulent drag in He-3-B is substantially higher at higher frequencies. We also present measurements of the inertial drag coefficient for grid turbulence in He-4. The inertial drag coefficient is significantly reduced by turbulence in both superfluid and normal liquid He-4

A fundamental investigation into aspects of the physiology and biochemistry of the stratum corneum in subjects with sensitive skin

BACKGROUND: Sensitive skin is a poorly understood skin condition. Defects in stratum corneum (SC) barrier function and/or extrasensory neuronal networks in the epidermis are believed to be involved in the problem. OBJECTIVES: This study aimed to unravel the relationships between bleomycin hydrolase (BH) and calpain-1 (C-1), pyrrolidone carboxylic acid (PCA) levels, corneocyte maturation, transglutaminase (TG) and plasmin activities on the cheeks of subjects with sensitive skin. METHODS: Forty-eight female Caucasian subjects, Fitzpatrick skin phototypes II-III, with self - perceived sensitive facial skin were assessed and underwent a capsaicin reactivity test. Expert grading of skin condition was conducted as well as measurement of transepidermal water loss (TEWL), skin capacitance, SC cohesion and SC integrity. BH, C-1 and plasmin activities were measured as well as PCA levels, plasmin and TG activity. Differential Nile red and involucrin immunostaining was performed to assess corneocyte maturation and size. RESULTS: 52% of the subjects reacted to capsaicin. There were no significant differences between the capsaicin-sensitive and non-capsaicin-sensitive subjects with reference to skin grading, TEWL, skin capacitance and SC cohesion. PCA levels and BH activity were lowest in the capsaicin-sensitive panel (p<0.05) and were correlated in non-capsaicin-sensitive subjects (r = 0.72). The activity of TG was significantly lower (48%) in the capsaicin-sensitive subjects (p<0.001) and their corneocytes were less mature and smaller (p ≤ 0.03). SC was estimated to be thinner (6.87 ± 0.28 vs. 8.68 ± 0.26 μm; p=0.001) in the capsaicin-sensitive subjects with a corresponding shorter SC path length (83.2± 4.4 μm and. 113.1 ± 4.5 μm; p=0.001). CONCLUSIONS: Despite the physiological similarities between the two groups of sensitive skin subjects, differences in their biochemistry were clearly evident. Lower levels of PCA, BH and TG activities together with a greater number of smaller and immature corneocytes indicate inferior SC maturation in the capsaicin-sensitive subjects. The reduced maturation of corneocytes and thinner SC likely contributes to a greater penetration of capsaicin and the associated increased skin sensitivity

Stable suspension and dispersion-induced transitions from repulsive Casimir forces between fluid-separated eccentric cylinders

Using an exact numerical method for finite nonplanar objects, we demonstrate a stable mechanical suspension of a silica cylinder within a metallic cylinder separated by ethanol, via a repulsive Casimir force between the silica and the metal. We investigate cylinders with both circular and square cross sections, and show that the latter exhibit a stable orientation as well as a stable position, via a method to compute Casimir torques for finite objects. Furthermore, the stable orientation of the square cylinder is shown to undergo an unusual 45 degrees transition as a function of the separation lengthscale, which is explained as a consequence of material dispersion.Comment: Published in Physical Review Letters. Vol. 101, page, 190404 (2008