27 research outputs found
The integration of dilute acid hydrolysis of xylan and fast pyrolysis of glucan to obtain fermentable sugars
Pretreatment and enzymatic process modification strategies to improve efficiency of sugar production from sugarcane bagasse
The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production
642 Risk of death after radical prostatectomy by pathological stage and grade for men with locally advanced prostate cancer
The role of Gleason score on survival of locally advanced (pT3) prostate cancer after radical prostatectomy
Survival after radical prostatectomy and external beam radiotherapy for patients with high risk prostate cancer: Results of single tertiary referral hospital
Hydrolytic potential of five fungal supernatants to enhance a commercial enzyme cocktail
Synthesis, characterization, spectral studies and antifungal activity of Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes with 3,3′-bis[N,N,di(carboxymethyl)-aminomethyl]- o
Visualization of structural changes in cellulosic substrates during enzymatic hydrolysis using multimodal nonlinear microscopy
Enzymatic hydrolysis of cellulose provides a renewable source of monosaccharides for production of variety of biochemicals and biopolymers. Unfortunately, the enzymatic hydrolysis of cellulose is often incomplete, and the reasons are not fully understood. We have monitored enzymatic hydrolysis in terms of molecular density, ordering and autofluorescence of cellulose structures in real time using simultaneous CARS, SHG and MPEF microscopy with the aim of contributing to the understanding and optimization of the enzymatic hydrolysis of cellulose. Three cellulose-rich substrates with different supramolecular structures, pulp fibre, acid-treated pulp fibre and Avicel, were studied at microscopic level. The microscopy studies revealed that before enzymatic hydrolysis Avicel had the greatest carbon-hydrogen density, while pulp fibre and acid-treated fibre had similar density. Monitoring of the substrates during enzymatic hydrolysis revealed the double exponential SHG decay for pulp fibre and acid-treated fibre indicating two phases of the process. Acid-treated fibre was hydrolysed most rapidly and the hydrolysis of pulp fibre was spatially non-uniform leading to fractioning of the particles, while the hydrolysis of Avicel was more than an order of magnitude slower than that of both fibres