23 research outputs found
WOUND HEALING EFFECT OF POLYHEXAMETHYLENE GUANIDINE HYDROCHLORIDE HYDROGELAT BURNS
This article considers the wound-healing effect of a hydrogel based on polyhexamethyleneguanidine hydrochloride under conditions of thermal shock modeling. The presence of gelling properties in combination with the high biocidal activity of polyguanidins opens the prospect of obtaining an effective single-component external agent, which is very important for antimicrobial chemotherapy of wounds and burns, as in their treatment there is a high probability of infecting the wound with microorganisms causing various infectious diseases. Previously, we developed a method for the preparation of a hydrogel based on polyhexamethyleneguanidine hydrochloride, which involves the cross-linking of the amino-end groups of the branched polymer with formaldehyde, resulting in a cross-linked polymer, which is a lump-like mass. The mechanical treatment of the hydrogel makes it possible to give the material an ointment form that is convenient for application to the skin. An experiment on the evaluation of the wound healing action of hydrogel was carried out under the conditions of modeling a thermal burn. The comparative preparation was multicomponent gel "Levomekol", in which polyethylene oxide was used as the basis. It was established that the hydrogel activates the growth of antioxidants and leukocytes in the blood of animals in dynamics on the 10th, 17th and 24th day of the experiment, amounting to 56.4 %, 33.8 % and 20.9 %, and of leukocytes - 27.7 %, 19.6 %; 6,2 % in relation to the norm. The method of contrast radiography of penetrating wounds showed that the daily reduction in the wound area is most pronounced in the hydrogel group, which indicates a pronounced reparative effect
Acute Toxicity of Hydrogel Polyhexamethylene Guanidine Hydrochloride
Background. Previously, we have shown that the polyhexamethylene guanidine hydrochloride hydrogel exhibits a pronounced wound healing. At the same time, no studies of the toxic effect of the hydrogel on animals have been conducted. Aim of the research. In the framework of this work, the acute toxicity of the hydrogel polyhexamethylene guanidine hydrochloride was studied in laboratory animals with intragastric administration. Materials and methods. The polyhexamethylene guanidine hydrochloride hydrogel was obtained by crosslinking the amino end groups with formaldehyde. An acute toxicity study was carried out (P 1.2.3156-13, GOST 32644-2014 and the Guidelines for conducting preclinical studies of drugs) in an experiment on outbred mice with a single addition of the test substance in different doses (1000, 3000, 5000, 8000 mg/kg) with fixing indicators (appearance, behavior, condition of the body hair coat, water and food consumption, excretion, body weight and its growth) during 14 days. After the animals were withdrawn from the experiment, autopsy, macroscopic evaluation and weighing of the internal organs were performed. The results showed that with the introduction of the test substance into the animal organism, death during the observation period (14 days) did not occur. It was not possible to determine the semi-lethal dose for the test compound. Conclusion. The conducted studies allow us to conclude that this substance is practically non-toxic and can be classified as hazard class V. Further research will be directed to the formation of hydrogel compositions with medicinal substances
Wound-healing effect of polyguanidine-based hydrogel
This article present the evaluation of polyhexamethyleneguanidine hydrochloride hydrogel influence on the course of wound healing processes. Synthesis of hydrogel is based on the interaction of amino-end groups of the polymer with carbonyl compounds. The presence of significant biocidal properties of the gel-forming polymer is allowed to consider the hydrogel as a wound healing agent. Since most wound healing agents for external use are made in the form of gels, ointments, polymer-based, it was decided to use the wound healing agent "Levomekol", a gel which consists of poly (ethylene oxide) as a comparison drug. So, in terms of linear skin-muscle wound we established the pronounced wound-healing effect of a polyhexamethyleneguanidine hydrochloride hydrogel. The results of pathomorphological studies revealed that in animals of the experimental "hydrogel" group on the eighth day of the experiment, the wound was undergoing a process of rejection of the scab, the presence of mature granulation tissue and no exudate was also observed. In the comparison group, the healing process was more slow, on the eighth day of the experiment in the animal group we observed the presence of fluid under the eschar, and lower amount of mature granulation tissue. Tensiometry of the scar has shown that the strength of scar rupture in the "hydrogel" group requires 65 % more force compared to the control group, while in the group of comparison this parameter made only 48 %
Π Π΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠ΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΠΎΠ»ΠΈΠ³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠΈΠ»Π΅Π½Π³ΡΠ°Π½ΠΈΠ΄ΠΈΠ½ Π³ΠΈΠ΄ΡΠΎΡ Π»ΠΎΡΠΈΠ΄Π°
Polyguanidines are characterized by pronounced antimicrobial activity and are widely used as the main active substance of disinfectants. Polymers of this class possess the property of gel formation, which, together with biological activity, opens the prospect of obtaining an effective single-component agent or basis for an external preparation for the therapy of skin lesions. Earlier, we developed a method for the production of a hydrogel based on polyhexamethyleneguanidine hydrochloride, which involves the cross-linking of the terminal amino groups of the branched polymer with formaldehyde.The purpose of the study was to observe the effect of hydrogel polyhexamethyleneguanidine hydrochloride on the progression of wound healing.Materials and methods. An experiment on the evaluation of the wound healing action of hydrogel was carried out under conditions simulating a linear cutaneous wound, a muscular wound, and a thermal burn. The comparative drug was the pharmacopoeian drug Levomecol.Results. It has been established that hydrogel has a pronounced wound healing effect, as evidenced by the results of ruminant thoracic anatomy and pathomorphology of the sections, which shows accelerated ripening of granulation tissue and scar formation. By the method of vulnografiya it was shown that the daily decrease in wound area is most pronounced in the hydrogel group, which also indicates a pronounced reparative effect. It was determined that the hydrogel activates the growth of antioxidatic activity and leukocytes in the blood of animals in dynamics on the 10th, 17th and 24th day of the experiment, amounting to 56, 34 and 21%, and of leukocytes β 32, 30, 10% in relation to the intact animals.Conclusion. Thus, on the model of a linear cutaneous wound, a muscular wound, and a thermal burn, the healing effect of the hydrogel PGMGh/f is established, as evidenced by the results of the early exercise, vulnografiya and pathomorphological studies. It is noted that the hydrogel PGMG h/f has an effect on the content of antioxidants and leukocytes in the blood, contributing to the normalization of their quantity.ΠΠΎΠ»ΠΈΠ³ΡΠ°Π½ΠΈΠ΄ΠΈΠ½Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΡΡ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ Π°Π½ΡΠΈΠΌΠΈΠΊΡΠΎΠ±Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΈ Π½Π°Ρ
ΠΎΠ΄ΡΡ ΡΠΈΡΠΎΠΊΠΎΠ΅ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΡΡΡΠ΅Π³ΠΎ Π²Π΅ΡΠ΅ΡΡΠ²Π° Π² ΡΠΎΡΡΠ°Π²Π΅ Π΄Π΅Π·ΠΈΠ½ΡΠΈΡΠΈΡΡΡΡΠΈΡ
ΡΡΠ΅Π΄ΡΡΠ². ΠΠΎΠ»ΠΈΠΌΠ΅ΡΡ ΡΡΠΎΠ³ΠΎ ΠΊΠ»Π°ΡΡΠ° ΠΎΠ±Π»Π°Π΄Π°ΡΡ ΡΠ²ΠΎΠΉΡΡΠ²ΠΎΠΌ Π³Π΅Π»Π΅ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ, ΡΡΠΎ Π² ΡΠΎΠ²ΠΎΠΊΡΠΏΠ½ΠΎΡΡΠΈ Ρ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΎΡΠΊΡΡΠ²Π°Π΅Ρ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΎΠ΄Π½ΠΎΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ΅Π΄ΡΡΠ²Π° ΠΈΠ»ΠΈ ΠΎΡΠ½ΠΎΠ²Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° Π½Π°ΡΡΠΆΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ Π΄Π»Ρ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΠΉ ΠΊΠΎΠΆΠ½ΡΡ
ΠΏΠΎΠΊΡΠΎΠ²ΠΎΠ². Π Π°Π½Π΅Π΅ Π½Π°ΠΌΠΈ Π±ΡΠ» ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½ ΡΠΏΠΎΡΠΎΠ± ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ ΠΏΠΎΠ»ΠΈΠ³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠΈΠ»Π΅Π½Π³ΡΠ°Π½ΠΈΠ΄ΠΈΠ½ Π³ΠΈΠ΄ΡΠΎΡ
Π»ΠΎΡΠΈΠ΄Π°, Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΠΈΠΉΡΡ Π² ΡΡΠΈΠ²Π°Π½ΠΈΠΈ ΠΊΠΎΠ½ΡΠ΅Π²ΡΡ
Π°ΠΌΠΈΠ½ΠΎΠ³ΡΡΠΏΠΏ ΡΠ°Π·Π²Π΅ΡΠ²Π»Π΅Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ° ΡΠΎΡΠΌΠ°Π»ΡΠ΄Π΅Π³ΠΈΠ΄ΠΎΠΌ.Π¦Π΅Π»ΡΡ ΡΠ°Π±ΠΎΡΡ ΡΠ²ΠΈΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²Π»ΠΈΡΠ½ΠΈΡ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΠΎΠ»ΠΈΠ³Π΅ΠΊΡΠ°ΠΌΠ΅ΡΠΈΠ»Π΅Π½Π³ΡΠ°Π½ΠΈΠ΄ΠΈΠ½ Π³ΠΈΠ΄ΡΠΎΡ
Π»ΠΎΡΠΈΠ΄Π° Π½Π° ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π½Π΅Π²ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½Ρ ΠΏΠΎ ΠΎΡΠ΅Π½ΠΊΠ΅ ΡΠ°Π½ΠΎΠ·Π°ΠΆΠΈΠ²Π»ΡΡΡΠ΅Π³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΡΡ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π»ΠΈΠ½Π΅ΠΉΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎ-ΠΌΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ°Π½Ρ ΠΈ ΡΠ΅ΡΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΠΆΠΎΠ³Π°. Π ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΡΡ ΡΠ°ΡΠΌΠ°ΠΊΠΎΠΏΠ΅ΠΉΠ½ΡΠΉ ΠΏΡΠ΅ΠΏΠ°ΡΠ°Ρ Β«ΠΠ΅Π²ΠΎΠΌΠ΅ΠΊΠΎΠ»ΡΒ».Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΎΠ±Π»Π°Π΄Π°Π» Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌ ΡΠ°Π½ΠΎΠ·Π°ΠΆΠΈΠ²Π»ΡΡΡΠΈΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ΠΌ Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ Π»ΠΈΠ½Π΅ΠΉΠ½ΠΎΠΉ ΡΠ°Π½Ρ, ΠΎ ΡΠ΅ΠΌ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ°Π½ΠΎΡΠ΅Π½Π·ΠΎΠΌΠ΅ΡΡΠΈΠΈ ΡΡΠ±ΡΠ° ΠΈ ΠΏΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΡΡΠ΅Π·ΠΎΠ², ΠΏΠΎΠΊΠ°Π·Π°Π²ΡΠΈΠ΅ ΡΡΠΊΠΎΡΠ΅Π½Π½ΠΎΠ΅ ΡΠΎΠ·ΡΠ΅Π²Π°Π½ΠΈΠ΅ Π³ΡΠ°Π½ΡΠ»ΡΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ ΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΡΡΠ±ΡΠ°. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ Π²ΡΠ»ΡΠ½ΠΎΠ³ΡΠ°ΡΠΈΠΈ Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠ΅ΡΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΠΆΠΎΠ³Π° ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΡΡΡΠΎΡΠ½ΠΎΠ΅ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΠ»ΠΎΡΠ°Π΄ΠΈ ΡΠ°Π½Ρ Π² Π½Π°ΠΈΠ±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΡΠΊΡΠΏΠ»ΠΈΡΠΈΡΠΎΠ²Π°Π½ΠΎ Ρ ΠΎΠΏΡΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ 1 (Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ), ΡΡΠΎ ΡΠ°ΠΊΠΆΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°Π»ΠΎ ΠΎ Π΅Π³ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΌ ΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΠΎΠΌ Π΄Π΅ΠΉΡΡΠ²ΠΈΠΈ. ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΠΎΠΆΠΎΠ³Π΅ Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΈΡΡ ΡΠ΅Π·ΠΊΠΎΠ΅ ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ²ΠΎΡΠΎΡΠΊΠΈ ΠΊΡΠΎΠ²ΠΈ ΠΈ Π½Π°ΡΠ°ΡΡΠ°Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ². Π Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ Π½Π° 10-, 17- ΠΈ 24-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° ΡΠΌΠ΅Π½ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΎ 56, 34 ΠΈ 21%, Π° ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π²ΡΠΎΡΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ β ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ Π½Π° 32, 30 ΠΈ 10% ΠΏΠΎ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΊ ΠΈΠ½ΡΠ°ΠΊΡΠ½ΡΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠΌ. ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎ, ΡΠ΅ΠΌ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠ° ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ, ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΠΎΠ²Π°Π»ΠΎ Π½ΠΎΡΠΌΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π΄Π°Π½Π½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π’Π°ΠΊΠΈΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ, Π½Π° ΠΌΠΎΠ΄Π΅Π»ΡΡ
Π»ΠΈΠ½Π΅ΠΉΠ½ΠΎΠΉ ΠΊΠΎΠΆΠ½ΠΎ-ΠΌΡΡΠ΅ΡΠ½ΠΎΠΉ ΡΠ°Π½Ρ ΠΈ ΡΠ΅ΡΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΠΆΠΎΠ³Π° ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ ΡΠ°Π½ΠΎΠ·Π°ΠΆΠΈΠ²Π»ΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ, ΠΎ ΡΠ΅ΠΌ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°Π»ΠΈ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡΒ ΡΠ°Π½ΠΎΡΠ΅Π½Π·ΠΎΠΌΠ΅ΡΡΠΈΠΈ, Π²ΡΠ»ΡΠ½ΠΎΠ³ΡΠ°ΡΠΈΠΈ ΠΏΠ°ΡΠΎΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ. ΠΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΈΠΉ ΡΠ°Π½ΠΎΠ·Π°ΠΆΠΈΠ²Π»ΡΡΡΠΈΠΉ ΡΡΡΠ΅ΠΊΡ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ, ΠΏΠΎ-Π²ΠΈΠ΄ΠΈΠΌΠΎΠΌΡ, ΡΠ²ΡΠ·Π°Π½ Ρ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠΌΠΌΠ°ΡΠ½ΠΎΠΉ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° Π»Π΅ΠΉΠΊΠΎΡΠΈΡΠΎΠ² Π² ΠΊΡΠΎΠ²ΠΈ
Preparation of aromatic polyamidines and their transformation in polybenzimidazoles
Polymers with amidine groups βNHβC(=NH)β in main chain were synthesized by two different approaches. The first strategy consists in polyaddition of dinitriles and diamines in acidic ionic liquids (ILs) which act as catalyst and solvent, while the second approach is based on polycondensation of 4,4'-oxybis(benzoic acid) diamide and diamines in Eatonβs reagent (ER). The resulting polyamidines (PADs) with Mw up to 25 000 g/mol possess thermal stability on air up to 288Β°C, and good solubility in polar organic solvents. Moreover dehydrocyclization of obtained PADs into polybenzimidazoles (PBIs) under the action of various oxidants was also studied in this work. The crosslinked films based on PBI and poly(amino imide) resin (PAIR) possess high mechanical characteristics. It has been proved that the crosslinked films based on PBI matrix are perspective materials for design the phosphoric acid electrolyte membranes for the medium temperature fuel cells
Reparative action of hydrogel polygexamethylenuanidine hydrochloride
Polyguanidines are characterized by pronounced antimicrobial activity and are widely used as the main active substance of disinfectants. Polymers of this class possess the property of gel formation, which, together with biological activity, opens the prospect of obtaining an effective single-component agent or basis for an external preparation for the therapy of skin lesions. Earlier, we developed a method for the production of a hydrogel based on polyhexamethyleneguanidine hydrochloride, which involves the cross-linking of the terminal amino groups of the branched polymer with formaldehyde.The purpose of the study was to observe the effect of hydrogel polyhexamethyleneguanidine hydrochloride on the progression of wound healing.Materials and methods. An experiment on the evaluation of the wound healing action of hydrogel was carried out under conditions simulating a linear cutaneous wound, a muscular wound, and a thermal burn. The comparative drug was the pharmacopoeian drug Levomecol.Results. It has been established that hydrogel has a pronounced wound healing effect, as evidenced by the results of ruminant thoracic anatomy and pathomorphology of the sections, which shows accelerated ripening of granulation tissue and scar formation. By the method of vulnografiya it was shown that the daily decrease in wound area is most pronounced in the hydrogel group, which also indicates a pronounced reparative effect. It was determined that the hydrogel activates the growth of antioxidatic activity and leukocytes in the blood of animals in dynamics on the 10th, 17th and 24th day of the experiment, amounting to 56, 34 and 21%, and of leukocytes β 32, 30, 10% in relation to the intact animals.Conclusion. Thus, on the model of a linear cutaneous wound, a muscular wound, and a thermal burn, the healing effect of the hydrogel PGMGh/f is established, as evidenced by the results of the early exercise, vulnografiya and pathomorphological studies. It is noted that the hydrogel PGMG h/f has an effect on the content of antioxidants and leukocytes in the blood, contributing to the normalization of their quantity