5,009 research outputs found
Quantitative performance characterization of three-dimensional noncontact fluorescence molecular tomography
© 2016 The Authors.Fluorescent proteins and dyes are routine tools for biological research to describe the behavior of genes, proteins, and cells, as well as more complex physiological dynamics such as vessel permeability and pharmacokinetics. The use of these probes in whole body in vivo imaging would allow extending the range and scope of current biomedical applications and would be of great interest. In order to comply with a wide variety of application demands, in vivo imaging platform requirements span from wide spectral coverage to precise quantification capabilities. Fluorescence molecular tomography (FMT) detects and reconstructs in three dimensions the distribution of a fluorophore in vivo. Noncontact FMT allows fast scanning of an excitation source and noninvasive measurement of emitted fluorescent light using a virtual array detector operating in free space. Here, a rigorous process is defined that fully characterizes the performance of a custom-built horizontal noncontact FMT setup. Dynamic range, sensitivity, and quantitative accuracy across the visible spectrum were evaluated using fluorophores with emissions between 520 and 660 nm. These results demonstrate that high-performance quantitative three-dimensional visible light FMT allowed the detection of challenging mesenteric lymph nodes in vivo and the comparison of spectrally distinct fluorescent reporters in cell culture
'I'm not X, I just want Y': formulating 'wants' in interaction
This article provides a conversation analytic description of a two-part structure, ‘I don’t want X, I want/just want Y’. Drawing on a corpus of recordings of family mealtimes and television documentary data, I show how speakers use the structure in two recurrent environments. First, speakers may use the structure to reject a proposal regarding their actions made by an interlocutor. Second, speakers may deliver the structure following a co-interactant’s formulation of their actions or motivations. Both uses decrease the likelihood of challenge in third-turn position. When responding to multi-unit turns speakers routinely deal with the last item first. The value of ‘I want Y’ is to formulate an alternative sense of agency which undermines the preceding turn and shifts the trajectory of the ongoing sequence. The article contributes to work in discursive psychology as I show how speakers may formulate their ‘wants’ in the service of sequentially unfolding social interaction
Tests of silicon sensors for the CMS pixel detector
The tracking system of the CMS experiment, currently under construction at
the Large Hadron Collider (LHC) at CERN (Geneva, Switzerland), will include a
silicon pixel detector providing three spacial measurements in its final
configuration for tracks produced in high energy pp collisions. In this paper
we present the results of test beam measurements performed at CERN on
irradiated silicon pixel sensors. Lorentz angle and charge collection
efficiency were measured for two sensor designs and at various bias voltages.Comment: Talk presented at 6th International Conference on Large Scale
Applications and Radiation Hardness of Semiconductor Detectors, September
29-October 1, 2003, Firenze, Italy. Proceedings will be published in Nuclear
Instr. & Methods in Phys. Research, Section
The hydrogen atom in an electric field: Closed-orbit theory with bifurcating orbits
Closed-orbit theory provides a general approach to the semiclassical
description of photo-absorption spectra of arbitrary atoms in external fields,
the simplest of which is the hydrogen atom in an electric field. Yet, despite
its apparent simplicity, a semiclassical quantization of this system by means
of closed-orbit theory has not been achieved so far. It is the aim of this
paper to close that gap. We first present a detailed analytic study of the
closed classical orbits and their bifurcations. We then derive a simple form of
the uniform semiclassical approximation for the bifurcations that is suitable
for an inclusion into a closed-orbit summation. By means of a generalized
version of the semiclassical quantization by harmonic inversion, we succeed in
calculating high-quality semiclassical spectra for the hydrogen atom in an
electric field
Properties of cage rearrangements observed near the colloidal glass transition
We use confocal microscopy to study the motions of particles in concentrated
colloidal systems. Near the glass transition, diffusive motion is inhibited, as
particles spend time trapped in transient ``cages'' formed by neighboring
particles. We measure the cage sizes and lifetimes, which respectively shrink
and grow as the glass transition approaches. Cage rearrangements are more
prevalent in regions with lower local concentrations and higher disorder.
Neighboring rearranging particles typically move in parallel directions,
although a nontrivial fraction move in anti-parallel directions, usually from
pairs of particles with initial separations corresponding to the local maxima
and minima of the pair correlation function , respectively.Comment: 5 pages, 4 figures; text & figures revised in v
Semiclassical quantization of the hydrogen atom in crossed electric and magnetic fields
The S-matrix theory formulation of closed-orbit theory recently proposed by
Granger and Greene is extended to atoms in crossed electric and magnetic
fields. We then present a semiclassical quantization of the hydrogen atom in
crossed fields, which succeeds in resolving individual lines in the spectrum,
but is restricted to the strongest lines of each n-manifold. By means of a
detailed semiclassical analysis of the quantum spectrum, we demonstrate that it
is the abundance of bifurcations of closed orbits that precludes the resolution
of finer details. They necessitate the inclusion of uniform semiclassical
approximations into the quantization process. Uniform approximations for the
generic types of closed-orbit bifurcation are derived, and a general method for
including them in a high-resolution semiclassical quantization is devised
Glasslike Arrest in Spinodal Decomposition as a Route to Colloidal Gelation
Colloid-polymer mixtures can undergo spinodal decomposition into colloid-rich
and colloid-poor regions. Gelation results when interconnected colloid-rich
regions solidify. We show that this occurs when these regions undergo a glass
transition, leading to dynamic arrest of the spinodal decomposition. The
characteristic length scale of the gel decreases with increasing quench depth,
and the nonergodicity parameter exhibits a pronounced dependence on scattering
vector. Mode coupling theory gives a good description of the dynamics, provided
we use the full static structure as input.Comment: 14 pages, 4 figures; replaced with published versio
Photoabsorption spectra of the diamagnetic hydrogen atom in the transition regime to chaos: Closed orbit theory with bifurcating orbits
With increasing energy the diamagnetic hydrogen atom undergoes a transition
from regular to chaotic classical dynamics, and the closed orbits pass through
various cascades of bifurcations. Closed orbit theory allows for the
semiclassical calculation of photoabsorption spectra of the diamagnetic
hydrogen atom. However, at the bifurcations the closed orbit contributions
diverge. The singularities can be removed with the help of uniform
semiclassical approximations which are constructed over a wide energy range for
different types of codimension one and two catastrophes. Using the uniform
approximations and applying the high-resolution harmonic inversion method we
calculate fully resolved semiclassical photoabsorption spectra, i.e.,
individual eigenenergies and transition matrix elements at laboratory magnetic
field strengths, and compare them with the results of exact quantum
calculations.Comment: 26 pages, 9 figures, submitted to J. Phys.
Significance of Ghost Orbit Bifurcations in Semiclassical Spectra
Gutzwiller's trace formula for the semiclassical density of states in a
chaotic system diverges near bifurcations of periodic orbits, where it must be
replaced with uniform approximations. It is well known that, when applying
these approximations, complex predecessors of orbits created in the bifurcation
("ghost orbits") can produce pronounced signatures in the semiclassical spectra
in the vicinity of the bifurcation. It is the purpose of this paper to
demonstrate that these ghost orbits themselves can undergo bifurcations,
resulting in complex, nongeneric bifurcation scenarios. We do so by studying an
example taken from the Diamagnetic Kepler Problem, viz. the period quadrupling
of the balloon orbit. By application of normal form theory we construct an
analytic description of the complete bifurcation scenario, which is then used
to calculate the pertinent uniform approximation. The ghost orbit bifurcation
turns out to produce signatures in the semiclassical spectrum in much the same
way as a bifurcation of real orbits would.Comment: 20 pages, 6 figures, LATEX (IOP style), submitted to J. Phys.
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