Lyophilized spore dispenser

A lyophilized spore dispenser is provided which produces a finely divided, monoparticulate cloud of bacterial spores. The spores are contained within a tightly sealed chamber, and a turbulator orifice connected to an air supply source provides a jet of air which stirs up the spores and causes the spores to be suspended in eddy currents within the chamber. This air jet also produces a positive pressure within the chamber which forces the spores out of an injection orifice

Letters between Emily C. Jessup and William Kerr\u27s secretary

Letters concerning a position in the music department at the Agricultural College

An Electrolysis Experiment for a Middle School Summer Science Camp

Higher education is often culturally deemphasized in the geographic area served by our rural, regional campus. As a result, faculty members have the opportunity to spearhead teaching efforts designed to educate the community about the importance of obtaining a post-secondary degree. To this end, we recently held a Science Summer Camp for middle school students, designed to infuse young people with an increased excitement for STEM (Science, Technology, Engineering, and Math) education. In this report, we summarize a chemical electrolysis experiment we carried out with middle school students for our annual Science Summer Camp. We also provided procedural guidelines for small- and large-scale experiments. In the latter case, evolved H2 gas can be detonated for effect. Two modifications from literature procedure include: (1) using glass burettes, instead of test tubes, to collect the evolving H2 and O2 gases for the small-scale setup; and (2) prefilling the 100-mL graduated collection cylinders with aqueous NaOH prior to beginning electrolysis. Because these modifications provide aqueous solution in the collection reservoirs prior to starting the experiment, the total time required for the experiment is greatly reduced (~30 minutes)

Evidence for Model-based Computations in the Human Amygdala during Pavlovian Conditioning

Contemporary computational accounts of instrumental conditioning have emphasized a role for a model-based system in which values are computed with reference to a rich model of the structure of the world, and a model-free system in which values are updated without encoding such structure. Much less studied is the possibility of a similar distinction operating at the level of Pavlovian conditioning. In the present study, we scanned human participants while they participated in a Pavlovian conditioning task with a simple structure while measuring activity in the human amygdala using a high-resolution fMRI protocol. After fitting a model-based algorithm and a variety of model-free algorithms to the fMRI data, we found evidence for the superiority of a model-based algorithm in accounting for activity in the amygdala compared to the model-free counterparts. These findings support an important role for model-based algorithms in describing the processes underpinning Pavlovian conditioning, as well as providing evidence of a role for the human amygdala in model-based inference

Development of measurement techniques for studying propeller erosion damage in severe wake fields

Preliminary propeller erosion tests have been conducted at the Naval Surface Warfare Center Carderock Division 24 inch variable pressure water tunnel (VPWT), shown in Figure 1, to establish testing procedures for evaluating various coatings to minimize cavitation erosion damage to marine propellers. A severe wake field was produced using a two dimensional, thick foil ahead of a downstream driven propeller model. This approach was derived from similar tests conducted by Miller [11]. Conventional cavitation viewing was performed with cameras viewing through the tunnel side window. Images were acquired using high speed (up to 6000 fps) and high resolution (2K x 2K) cameras. In addition, a waterproof camera was mounted inside the foil looking directly downstream at the suction face of the blade. Two propellers were tested, a 16 inch (0.406 m) diameter propeller 5388 and a 12 inch (0.305 m) diameter propeller 4119 [8]. The foil wake field was measured with LDV surveys. Accelerometers were mounted in the water tunnel test section to measure acoustic emissions of cavitation activity. Cavitation erosion was observed at the tip of the 16 inch diameter propeller due to excessive tip vortex, and complicated vortex collapse. Moderate erosion was also observed at the inner radii, where leading edge sheet cavitation collapsed. Scanning techniques for quantifying propeller erosion damage were evaluated. These studies will transition to the 36-inch VPWT where a number of geosym propellers of different materials and coating will be assessed in a similar wake field.http://deepblue.lib.umich.edu/bitstream/2027.42/84210/1/CAV2009-final156.pd

My Care, I Care: A study of what people with HIV value about NHS HIV services across London

Duration: January 2012 - March 2013 As part of a larger review of HIV services in London, the London Specialised Commissioning Group (SCG) commissioned Sigma Research to investigate the views of people with diagnosed HIV about the London NHS HIV services provided to them. The final report from the research, which included a large-scale self-completion survey and eight focus groups, is available to download here. It is also available in printed format, free to UK addresses here. Almost 1400 people with diagnosed HIV self-completion the survey which was available in HIV clinics and online from early February to the end of July 2012. The survey covered: demographics, current service use, motivations for service choice, aspirations for service development and broader social care needs. Sampling and recruitment were controlled in an attempt to ensure a sample broadly representative of the population of people using HIV clinical services in London and to ensure representation from all 28 London clinics. The survey was promoted online by HIV organisations and some HIV clinics. In addition paper copies of the survey were administered by dedicated fieldworkers in all the 28 specialist HIV out-patients clinics in Greater London. Eight focus groups with key groups of people with HIV were used to investigate in more detail motivations for service use, satisfaction with services and aspirations and suggestions for service development

Regional differences in nutrient-induced secretion of gut serotonin

Enterochromaffin (EC) cells located in the gastrointestinal (GI) tract provide the vast majority of serotonin (5-HT) in the body and constitute half of all enteroendocrine cells. EC cells respond to an array of stimuli, including various ingested nutrients. Ensuing 5-HT release from these cells plays a diverse role in regulating gut motility as well as other important responses to nutrient ingestion such as glucose absorption and fluid balance. Recent data also highlight the role of peripheral 5-HT in various pathways related to metabolic control. Details related to the manner by which EC cells respond to ingested nutrients are scarce and as that the nutrient environment changes along the length of the gut, it is unknown whether the response of EC cells to nutrients is dependent on their GI location. The aim of the present study was to identify whether regional differences in nutrient sensing capability exist in mouse EC cells. We isolated mouse EC cells from duodenum and colon to demonstrate differential responses to sugars depending on location. Measurements of intracellular calcium concentration and 5-HT secretion demonstrated that colonic EC cells are more sensitive to glucose, while duodenal EC cells are more sensitive to fructose and sucrose. Short-chain fatty acids (SCFAs), which are predominantly synthesized by intestinal bacteria, have been previously associated with an increase in circulating 5-HT; however, we find that SCFAs do not acutely stimulate EC cell 5-HT release. Thus, we highlight that EC cell physiology is dictated by regional location within the GI tract, and identify differences in the regional responsiveness of EC cells to dietary sugars.Alyce M. Martin, Amanda L. Lumsden, Richard L. Young, Claire F. Jessup, Nick J. Spencer, Damien J. Keatin

Backyard Battles: Local Struggles for African American Political Advancement in Baltimore, 1920-1944.

ABSTRACT BACKYARD BATTLES: LOCAL STRUGGLES FOR AFRICAN AMERICAN POLITICAL ADVANCEMENT IN BALTIMORE, 1920-1944 by Angelique D. Jessup Co-Chairs: Nancy E. Burns and Robert W. Mickey This dissertation discusses the development of African American activism and political participation in Baltimore from 1920–1944 through different issue domains, and looks especially to when activists developed a complementary fit between their existing resources, their political environment, and their mode of mobilization. This project is motivated by some important unanswered questions in political science literature. First, several studies investigating black political attitudes and behavior begin with an analysis of black political behavior during or after the mainstream Civil Rights Movement. This literature does not offer a full picture of the nature of black political engagement prior to the big structural changes occurring during and after the mainstream Civil Rights Movement, and even less about the potential for local activism to set the stage for organizing work in later periods. Scholars in American Political Development (APD) have made great strides in explaining the institutional underpinnings of racial policy change, but do not reveal why black activists could not make larger gains without changes to structural conditions. This project demonstrates how black activists made strategic attempts to position their expertise and skill set to exploit available openings in local politics. Additionally, it shows how black activists utilized different sets of organizing structures and that different modes of organizing were consequential to the tactics unleashed and the issues they fought for. This project also works to show that black activists amassed resources in intricate and innovative ways to bolster their political influence and strengthen their leverage with local policymakers. By turning to the local politics of blacks during this period, this project brings attention to the tactics and strategies that later set the groundwork for bigger battles during the Civil Rights Movement.Ph.D.Political ScienceUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/75925/1/adouyon_1.pd