Intravital Microscopy of Tumor Oxygenation and Glycolytic Demand

There is growing concern about the over treatment of cancer because treatments are based primarily on tumor anatomy. In this study, we aim to begin the process of addressing that issue by developing an intravital technique for optically analyzing tumor biology. Two traits were identified as having significant importance in the aggression of a tumor, vascular oxygenation (SO2) and glycolytic demand. Dorsal skin flap window chambers were implanted and 4T1 and 67NR cancer cells were injected to provide a tumor model for the development of this intravital quantification technique. This study provides a detailed protocol from instrumentation setup to surgical procedures to imaging methods as well as showing that optical techniques can be used to quantify SO2 and glycolytic demand within various cell lines

A time-stepping dynamically-consistent spherical-shell dynamo code

A pseudo-spectral dynamo code, developed as a computational laboratory, is described. The magnetic, heat and Boussinesq Navier-Stokes equations, with inertia, non-linear advection, buoyancy with asymmetric gravity, Coriolis, viscous and Lorentz forces, are solved numerically in a rotating conducting fluid shell. The convection is thermally driven by prescribed boundary temperatures. The equations are discretised using toroidal-poloidal fields, Chebychev collocation in radius and spherical harmonic expansion in angles. Derivatives are performed spectrally. Products are evaluated in physical space for efficiency. Fields are transformed between physical and spectral spaces by fast Fourier and Gauss-Legendre methods. Linear terms are time-stepped implicitly and product terms explicitly using an Adams predictor/corrector. Results are presented for two benchmark models

Morning quiet-time ionospheric current reversal at mid to high latitudes

The records of an array of magnetometers set up across the Australian mainland are examined. In addition to a well-defined current whorl corresponding to the ionospheric <i>S<sub>q</sub></i> current system, another system of eastward flowing currents is often found in the early morning. The system is most easily identified at observatories poleward of the focus of the <i>S<sub>q</sub></i> system, where a morning reversal from eastward to westward currents can be seen. The time of the reversal is usually later, sometimes up to 12h local noon, in June (Southern Winter) than in other seasons. There is some evidence of a similar current system at other longitudes and in the Northern Hemisphere. An important outcome of the study is that it enables identification of which features of a daily variation of the northward magnetic field Δ<i>X</i> relate to an <i>S<sub>q</sub></i> current whorl and which must be attributed to some other current system

Magnetohydrodynamic activity inside a sphere

We present a computational method to solve the magnetohydrodynamic equations in spherical geometry. The technique is fully nonlinear and wholly spectral, and uses an expansion basis that is adapted to the geometry: Chandrasekhar-Kendall vector eigenfunctions of the curl. The resulting lower spatial resolution is somewhat offset by being able to build all the boundary conditions into each of the orthogonal expansion functions and by the disappearance of any difficulties caused by singularities at the center of the sphere. The results reported here are for mechanically and magnetically isolated spheres, although different boundary conditions could be studied by adapting the same method. The intent is to be able to study the nonlinear dynamical evolution of those aspects that are peculiar to the spherical geometry at only moderate Reynolds numbers. The code is parallelized, and will preserve to high accuracy the ideal magnetohydrodynamic (MHD) invariants of the system (global energy, magnetic helicity, cross helicity). Examples of results for selective decay and mechanically-driven dynamo simulations are discussed. In the dynamo cases, spontaneous flips of the dipole orientation are observed.Comment: 15 pages, 19 figures. Improved figures, in press in Physics of Fluid

Statewide retrospective study of low acuity emergency presentations in New South Wales, Australia: who, what, where and why?

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/Abstract Objective The present study aims to use a statewide population-based registry to assess the prevalence of low acuity emergency department (ED) presentations, describe the trend in presentation rates and to determine whether they were associated with various presentation characteristics such as the type of hospital as well as clinical and demographic variables. Design and setting This was a retrospective analysis of a population-based registry of ED presentations in New South Wales (NSW). Generalised estimating equations with log links were used to determine factors associated with low acuity presentations to account for repeat presentations and the possibility of clustering of outcomes. Participants Patients were included in this analysis if they presented to an ED between January 2010 and December 2014. The outcomes of interest were low acuity presentation, defined as those who self-presented (were not transported by ambulance), were assigned a triage category of 4 or 5 (semiurgent or non-urgent) and discharged back to usual residence from ED. Results There were 10.7 million ED presentations analysed. Of these, 45% were classified as a low acuity presentation. There was no discernible increase in the rate of low acuity presentations across NSW between 2010 and 2014. The strongest predictors of low acuity ED presentation were age <40 years of age (OR 1.77); injury or musculoskeletal administrative and non-urgent procedures (OR 2.96); ear, nose and throat, eye or oral (OR 5.53); skin or allergy-type presenting problems (OR 2.84). Conclusions Low acuity ED presentations comprise almost half of all ED presentations. Alternative emergency models of care may help meet the needs of these patients

A vector spherical harmonic spectral code for linearised magnetohydrodynamics

Linearised rotating magnetohydrodynamic stability code for the steady axisymmetric basic states of an electrically conducting fluid sphere is described. The code generates compact hybrid angular spectral forms of the magnetic induction, heat and Boussinesq Navier-Stokes equations, using toroidal and poloidal representations of the perturbation vector fields, and vector or scalar spherical harmonic expansions of all fields. The momentum equation may include inertial, Coriolis, buoyancy, viscous and magnetic Lorentz forces. Three subroutines evaluate the spectral interactions of products. There are only six radial functions, which are discretised using uniform second-order finite differences. The resulting large scale complex non-hermitian generalised eigen- and critical-value problems are solved using inverse and Newton-Raphson iteration methods, respectively. Test results are presented for several models

Optimization of Material Contrast for Efficient FIB‐SEM Tomography of Solid Oxide Fuel Cells

Focused ion beam (FIB) – scanning electron microscopy (SEM) serial sectioning tomography has become an important tool for three‐dimensional microstructure reconstruction of solid oxide fuel cells (SOFC) to obtain an understanding of fabrication‐related effects and SOFC performance. By sequential FIB milling and SEM imaging a stack of cross‐section images across all functional SOFC layers was generated covering a large volume of 3.5·10$^{4}$ μm$^{3}$. One crucial step is image segmentation where regions with different image intensities are assigned to different material phases within the SOFC. To analyze all relevant SOFC materials, it was up to now mandatory to acquire several images by scanning the same region with different imaging parameters because sufficient material contrast could otherwise not be achieved. In this work we obtained high‐contast SEM images from a single scan to reconstract all functional SOFC layers consisting of a Ni/Y$_{2}$O$_{3}$‐doped ZrO$_{2}$ (YDZ) cermet anode, YDZ electrolyte and (La,Sr)MnO$_{3}$/YDZ cathode. This was possible by using different, simultaneous read‐out detectors installed in a state‐of‐the‐art scanning electron microscope. In addition, we used a deterministic approach for the optimization of imaging parameters by employing Monte Carlo simulations rather than trial‐and‐error tests. We also studied the effect of detection geometry, detecting angle range and detector type