Evolved Eclipsing Binaries and the Age of the Open Cluster NGC 752

We present analyses of improved photometric and spectroscopic observations for two detached eclipsing binaries at the turnoff of the open cluster NGC 752: the 1.01 day binary DS And and the 15.53 d BD $+$37 410. For DS And, we find $M_1 = 1.692\pm0.004\pm0.010 M_\odot$, $R_1 = 2.185\pm0.004\pm0.008 R_\odot$, $M_2 = 1.184\pm0.001\pm0.003 M_\odot$, and $R_2 = 1.200\pm0.003\pm0.005 R_\odot$. We either confirm or newly identify unusual characteristics of both stars in the binary: the primary star is found to be slightly hotter than the main sequence turn off and there is a more substantial discrepancy in its luminosity compared to models (model luminosities are too large by about 40%), while the secondary star is oversized and cooler compared to other main sequence stars in the same cluster. The evidence points to non-standard evolution for both stars, but most plausible paths cannot explain the low luminosity of the primary star. BD $+$37 410 only has one eclipse per cycle, but extensive spectroscopic observations and the TESS light curve constrain the stellar masses well: $M_1 = 1.717\pm0.011 M_\odot$ and $M_2 = 1.175\pm0.005 M_\odot$. The radius of the main sequence primary star near $2.9R_\odot$ definitively requires large convective core overshooting ($> 0.2$ pressure scale heights) in models for its mass, and multiple lines of evidence point toward an age of $1.61\pm0.03\pm0.05$ Gyr (statistical and systematic uncertainties). Because NGC 752 is currently undergoing the transition from non-degenerate to degenerate He ignition of its red clump stars, BD $+$37 410 A directly constrains the star mass where this transition occurs.Comment: 34 pages, 23 figures, accepted for Astronomical Journa

Non-invasive detection of animal nerve impulses with an atomic magnetometer operating near quantum limited sensitivity

Magnetic fields generated by human and animal organs, such as the heart, brain and nervous system carry information useful for biological and medical purposes. These magnetic fields are most commonly detected using cryogenically-cooled superconducting magnetometers. Here we present the frst detection of action potentials from an animal nerve using an optical atomic magnetometer. Using an optimal design we are able to achieve the sensitivity dominated by the quantum shot noise of light and quantum projection noise of atomic spins. Such sensitivity allows us to measure the nerve impulse with a miniature room-temperature sensor which is a critical advantage for biomedical applications. Positioning the sensor at a distance of a few millimeters from the nerve, corresponding to the distance between the skin and nerves in biological studies, we detect the magnetic field generated by an action potential of a frog sciatic nerve. From the magnetic field measurements we determine the activity of the nerve and the temporal shape of the nerve impulse. This work opens new ways towards implementing optical magnetometers as practical devices for medical diagnostics.Comment: Main text with figures, and methods and supplementary informatio

A Robust Measure of Tidal Circularization in Coeval Binary Populations: The solar-type spectroscopic Binary Population in The Open Cluster M35

We present a new homogeneous sample of 32 spectroscopic binary orbits in the young (~ 150 Myr) main-sequence open cluster M35. The distribution of orbital eccentricity vs. orbital period (e-log(P)) displays a distinct transition from eccentric to circular orbits at an orbital period of ~ 10 days. The transition is due to tidal circularization of the closest binaries. The population of binary orbits in M35 provide a significantly improved constraint on the rate of tidal circularization at an age of 150 Myr. We propose a new and more robust diagnostic of the degree of tidal circularization in a binary population based on a functional fit to the e-log(P) distribution. We call this new measure the tidal circularization period. The tidal circularization period of a binary population represents the orbital period at which a binary orbit with the most frequent initial orbital eccentricity circularizes (defined as e = 0.01) at the age of the population. We determine the tidal circularizationperiod for M35 as well as for 7 additional binary populations spanning ages from the pre main-sequence (~ 3 Myr) to late main-sequence (~ 10 Gyr), and use Monte Carlo error analysis to determine the uncertainties on the derived circularization periods. We conclude that current theories of tidal circularization cannot account for the distribution of tidal circularization periods with population age.Comment: 37 pages, 9 figures, to be published in The Astrophysical Journal, February 200

Solar-like oscillations in the G2 subgiant beta Hydri from dual-site observations

We have observed oscillations in the nearby G2 subgiant star beta Hyi using high-precision velocity observations obtained over more than a week with the HARPS and UCLES spectrographs. The oscillation frequencies show a regular comb structure, as expected for solar-like oscillations, but with several l=1 modes being strongly affected by avoided crossings. The data, combined with those we obtained five years earlier, allow us to identify 28 oscillation modes. By scaling the large frequency separation from the Sun, we measure the mean density of beta Hyi to an accuracy of 0.6%. The amplitudes of the oscillations are about 2.5 times solar and the mode lifetime is 2.3 d. A detailed comparison of the mixed l=1 modes with theoretical models should allow a precise estimate of the age of the star.Comment: 13 pages, 14 figures, accepted by ApJ. Fixed minor typo (ref to Fig 14

Spatial Frequency Domain Tomography of Protoporphyrin IX Fluorescence in Preclinical Glioma Models

Multifrequency (0 to 0.3  mm−1), multiwavelength (633, 680, 720, 800, and 820 nm) spatial frequency domain imaging (SFDI) of 5-aminolevulinic acid-induced protoporphyrin IX (PpIX) was used to recover absorption, scattering, and fluorescence properties of glioblastoma multiforme spheroids in tissue-simulating phantoms and in vivo in a mouse model. Three-dimensional tomographic reconstructions of the frequency-dependent remitted light localized the depths of the spheroids within 500 μm, and the total amount of PpIX in the reconstructed images was constant to within 30% when spheroid depth was varied. In vivo tumor-to-normal contrast was greater than ∼ 1.5 in reduced scattering coefficient for all wavelengths and was ∼ 1.3 for the tissue concentration of deoxyhemoglobin (ctHb). The study demonstrates the feasibility of SFDI for providing enhanced image guidance during surgical resection of brain tumors

Release of functional peptides from mother's milk and fortifier proteins in the premature infant stomach

Digestion of milk proteins in the premature infant stomach releases functional peptides; however, which peptides are present has not been reported. Premature infants are often fed a combination of human milk and bovine milk fortifiers, but the variety of functional peptides released from both human and bovine milk proteins remains uncharacterized. This study applied peptidomics to investigate the peptides released in gastric digestion of mother's milk proteins and supplemental bovine milk proteins in premature infants. Peptides were assessed for homology against a database of known functional peptides-Milk Bioactive Peptide Database. The peptidomic data were analyzed to interpret which proteases most likely released them from the parent protein. We identified 5,264 unique peptides from bovine and human milk proteins within human milk, fortifier or infant gastric samples. Plasmin was predicted to be the most active protease in milk, while pepsin or cathepsin D were predicted to be most active in the stomach. Alignment of the peptide distribution showed a different digestion pattern between human and bovine proteins. The number of peptides with high homology to known functional peptides (antimicrobial, angiotensin-converting enzyme-inhibitory, antioxidant, immunomodulatory, etc.) increased from milk to the premature infant stomach and was greater from bovine milk proteins than human milk proteins. The differential release of bioactive peptides from human and bovine milk proteins may impact overall health outcomes in premature infants

Narrow optical linewidths and spin pumping on charge-tunable close-to-surface self-assembled quantum dots in an ultrathin diode

We demonstrate full charge control, narrow optical linewidths, and optical spin pumping on single self-assembled InGaAs quantum dots embedded in a 162.5−nm-thin diode structure. The quantum dots are just 88nm from the top GaAs surface. We design and realize a p−i−n−i−n diode that allows single-electron charging of the quantum dots at close-to-zero applied bias. In operation, the current flow through the device is extremely small resulting in low noise. In resonance fluorescence, we measure optical linewidths below 2μeV, just a factor of 2 above the transform limit. Clear optical spin pumping is observed in a magnetic field of 0.5T in the Faraday geometry. We present this design as ideal for securing the advantages of self-assembled quantum dots—highly coherent single-photon generation, ultrafast optical spin manipulation—in the thin diodes required in quantum nanophotonics and nanophononics applications

A Transiting Hot Jupiter Orbiting a Metal-Rich Star

We announce the discovery of Kepler-6b, a transiting hot Jupiter orbiting a star with unusually high metallicity, [Fe/H] = +0.34 +/- 0.04. The planet's mass is about 2/3 that of Jupiter, Mp = 0.67 Mj, and the radius is thirty percent larger than that of Jupiter, Rp = 1.32 Rj, resulting in a density of 0.35 g/cc, a fairly typical value for such a planet. The orbital period is P = 3.235 days. The host star is both more massive than the Sun, Mstar = 1.21 Msun, and larger than the Sun, Rstar = 1.39 Rsun.Comment: 12 pages, 2 figures, submitted to the Astrophysical Journal Letter

Pacing with restoration of respiratory sinus arrhythmia improved cardiac contractility and the left ventricular output: a translational study

Introduction: Respiratory sinus arrhythmia (RSA) is a prognostic value for patients with heart failure and is defined as a beat-to-beat variation of the timing between the heart beats. Patients with heart failure or patients with permanent cardiac pacing might benefit from restoration of RSA. The aim of this translational, proof-of-principle study was to evaluate the effect of pacing with or without restored RSAon parameters of LV cardiac contractility and the cardiac output

The Domain-Specific and Temperature-Dependent Protein Misfolding Phenotype of Variant Medium-Chain acyl-CoA Dehydrogenase

The implementation of expanded newborn screening programs reduced mortality and morbidity in medium-chain acyl-CoA dehydrogenase deficiency (MCADD) caused by mutations in the ACADM gene. However, the disease is still potentially fatal. Missense induced MCADD is a protein misfolding disease with a molecular loss-of-function phenotype. Here we established a comprehensive experimental setup to analyze the structural consequences of eight ACADM missense mutations (p. Ala52Val, p. Tyr67His, p. Tyr158His, p. Arg206Cys, p. Asp266Gly, p. Lys329Glu, p. Arg334Lys, p. Arg413Ser) identified after newborn screening and linked the corresponding protein misfolding phenotype to the site of side-chain replacement with respect to the domain. With fever being the crucial risk factor for metabolic decompensation of patients with MCADD, special emphasis was put on the analysis of structural and functional derangements related to thermal stress. Based on protein conformation, thermal stability and kinetic stability, the molecular phenotype in MCADD depends on the structural region that is affected by missense-induced conformational changes with the central beta-domain being particularly prone to structural derangement and destabilization. Since systematic classification of conformational derangements induced by ACADM mutations may be a helpful tool in assessing the clinical risk of patients, we scored the misfolding phenotype of the variants in comparison to p. Lys329Glu (K304E),the classical severe mutation, and p. Tyr67His (Y42H),discussed to be mild. Experiments assessing the impact of thermal stress revealed that mutations in the ACADM gene lower the temperature threshold at which MCAD loss-of-function occurs. Consequently, increased temperature as it occurs during intercurrent infections, significantly increases the risk of further conformational derangement and loss of function of the MCAD enzyme explaining the life-threatening clinical courses observed during fever episodes. Early and aggressive antipyretic treatment thus may be life-saving in patients suffering from MCADD