Quality of life in children with psychiatric disorders

The objective of the study described in this thesis was to examine several aspects of quality of life (QoL) of children with psychiatric disorders. In Chapter 1, the background and the main aims were presented. Because treatment effects for severe and chronic diseases have been improved in the last decades, QoL emerged as an important outcome measure. For the present thesis, a longitudinal study with two assessments across a one-year follow-up period was conducted among 310 children and adolescents (aged 6-18 years) who had been referred to outpatient mental health agencies. Besides, a community sample of 74 children without psychiatric problems was included. The aims of this study were: (1) to assess the usefulness of the Pediatric Quality of Life Inventory (PedsQL) as an instrument measuring QoL in children with psychiatric problems, (2) to compare QoL of children with psychiatric problems to QoL of children from the general population without psychiatric problems, (3) to compare QoL of children with different psychiatric disorders, (4) to identify factors that are associated with QoL, (5) to determine the course of QoL, and (6) to identify predictors of change in QoL across time

Psychopathology in children: Improvement of quality of life without psychiatric symptom reduction

Objective: The aim of this study was to assess the association between change in psychopathology and Quality of Life (QoL) across time in children with high levels of psychopathology. Methods: A referred sample of 126 seven- to 19-year-olds was studied across a 1-year follow-up period. Information concerning QoL and psychopathology was obtained from parents. Results: Overall, 38.1% of children showed neither psychiatric symptom reduction nor QoL improvement, 33.3% of children showed both a clinically significant psychiatric symptom reduction and QoL improvement, and 28.6% of children showed either psychiatric symptom reduction or QoL improvement. In 11.1% of all children,QoL improved, while the level of psychopathology remained high. Age, gender, or psychiatric diagnosis did not predict a poor outcome of persistently high psychopathology scores and poor QoL. Conclusion: QoL in children with psychiatric problems may be improved by reducing psychiatric symptoms in a number of children, but it is also possible to improve QoL without psychiatric symptom reduction. This implicates that QoL should become an important aim and treatment outcome measure of psychiatric treatment programs, especially since psychopathology tends to persist. © Steinkopff Verlag 2005

Artificial flagella: bio-inspired responsive polymers for micro-object manipulation

No abstract

Correcting for the Effects of Interstellar Extinction

This paper addresses the issue of how best to correct astronomical data for the wavelength-dependent effects of Galactic interstellar extinction. The main general features of extinction from the IR through the UV are reviewed, along with the nature of observed spatial variations. The enormous range of extinction properties found in the Galaxy, particularly in the UV spectral region, is illustrated. Fortunately, there are some tight constraints on the wavelength dependence of extinction and some general correlations between extinction curve shape and interstellar environment. These relationships provide some guidance for correcting data for the effects of extinction. Several strategies for dereddening are discussed along with estimates of the uncertainties inherent in each method. In the Appendix, a new derivation of the wavelength dependence of an average Galactic extinction curve from the IR through the UV is presented, along with a new estimate of how this extinction law varies with the parameter R = A(V)/E(B-V). These curves represent the true monochromatic wavelength dependence of extinction and, as such, are suitable for dereddening IR--UV spectrophotometric data of any resolution, and can be used to derive extinction relations for any photometry system.Comment: To appear in PASP (January 1999) 14 pages including 4 pages of figures Uses emulateapj style. PASP, in press (January 1999

New Alleles in the “RN gene” Associated with Low Glycogen Content in Pig Skeletal Muscle and Improved Meat Quality

Several quantitative trait loci (QTL) affecting muscle glycogen content and related meat quality traits were mapped to pig chromosome 15 (SSC 15) by using a three-generation intercross between Berkshire x Yorkshire pigs. Based on the QTL location, the PRKAG3 (protein kinase AMPactivated, γ3 subunit) or the RN (Rendement Napole) gene, as it is popularly known, was considered to be a good candidate for the observed effects. Differences in the PRKAG3 gene sequences of the founder animals of the intercross were analyzed. The RN- mutation previously reported was not present in the cross but three missense substitutions (changes affecting proteins) were identified. To test the hypothesis that at least one of these mutations was associated with differences in meat quality, more than 1,800 animals from several unrelated commercial lines were genotyped for the candidate gene differences and an association study was performed. The results provide strong support for the presence of new economically important alleles (gene forms) of the PRKAG3 gene affecting the glycogen content in the muscle and the resulting meat quality. Because of their prevalence in the more common commercial breeds, the potential implications for the pig industry and consumers are probably more important than the original discovery of the RN- mutation

New alleles in calpastatin gene are associated with meat quality traits in pigs

Suggestive QTL affecting raw firmness scores and average Instron force, tenderness, juiciness, and chewiness on cooked meat were mapped to pig chromosome 2 using a three-generation intercross between Berkshire and Yorkshire pigs. Based on its function and location, the calpastatin (CAST) gene was considered to be a good candidate for the observed effects. Several missense and silent mutations were identified in CAST and haplotypes covering most of the coding region were constructed and used for association analyses with meat quality traits. Results demonstrated that one CAST haplotype was significantly associated with lower Instron force and cooking loss and higher juiciness and, therefore, this haplotype is associated with higher eating quality. Some of the sequence variation identified may be associated with differences in phosphorylation of CAST by adenosine cyclic 3′, 5′-monophosphate- dependent protein kinase and may in turn explain the meat quality phenotypic differences. The beneficial haplotype was present in all the commercial breeds tested and may provide significant improvements for the pig industry and consumers because it can be used in marker-assisted selection to produce naturally tender and juicy pork without additional processing steps

Motion-resolved fat-fraction mapping with whole-heart free-running multiecho GRE and pilot tone.

To develop a free-running 3D radial whole-heart multiecho gradient echo (ME-GRE) framework for cardiac- and respiratory-motion-resolved fat fraction (FF) quantification. (N <sub>TE</sub> = 8) readouts optimized for water-fat separation and quantification were integrated within a continuous non-electrocardiogram-triggered free-breathing 3D radial GRE acquisition. Motion resolution was achieved with pilot tone (PT) navigation, and the extracted cardiac and respiratory signals were compared to those obtained with self-gating (SG). After extra-dimensional golden-angle radial sparse parallel-based image reconstruction, FF, R <sub>2</sub> *, and B <sub>0</sub> maps, as well as fat and water images were generated with a maximum-likelihood fitting algorithm. The framework was tested in a fat-water phantom and in 10 healthy volunteers at 1.5 T using N <sub>TE</sub> = 4 and N <sub>TE</sub> = 8 echoes. The separated images and maps were compared with a standard free-breathing electrocardiogram (ECG)-triggered acquisition. The method was validated in vivo, and physiological motion was resolved over all collected echoes. Across volunteers, PT provided respiratory and cardiac signals in agreement (r = 0.91 and r = 0.72) with SG of the first echo, and a higher correlation to the ECG (0.1% of missed triggers for PT vs. 5.9% for SG). The framework enabled pericardial fat imaging and quantification throughout the cardiac cycle, revealing a decrease in FF at end-systole by 11.4% ± 3.1% across volunteers (p < 0.0001). Motion-resolved end-diastolic 3D FF maps showed good correlation with ECG-triggered measurements (FF bias of -1.06%). A significant difference in free-running FF measured with N <sub>TE</sub> = 4 and N <sub>TE</sub> = 8 was found (p < 0.0001 in sub-cutaneous fat and p < 0.01 in pericardial fat). Free-running fat fraction mapping was validated at 1.5 T, enabling ME-GRE-based fat quantification with N <sub>TE</sub> = 8 echoes in 6:15 min

Nano-second laser interference photoembossed microstructures for enhanced cell alignment

Photoembossing is a powerful photolithographic technique to prepare surface relief structures relying on polymerization-induced diffusion in a solventless development step. Conveniently, surface patterns are formed by two or more interfering laser beams without the need for a lithographic mask. The use of nanosecond pulsed light-based interference lithography strengthens the pattern resolution through the absence of vibrational line pattern distortions. Typically, a conventional photoembossing protocol consists of an exposure step at room temperature that is followed by a thermal development step at high temperature. In this work, we explore the possibility to perform the pulsed holographic exposure directly at the development temperature. The surface relief structures generated using this modified photoembossing protocol are compared with those generated using the conventional one. Importantly, the enhancement of surface relief height has been observed by exposing the samples directly at the development temperature, reaching approximately double relief heights when compared to samples obtained using the conventional protocol. Advantageously, the light dose needed to reach the optimum height and the amount of photoinitiator can be substantially reduced in this modified protocol, demonstrating it to be a more efficient process for surface relief generation in photopolymers. Kidney epithelial cell alignment studies on substrates with relief-height optimized structures generated using the two described protocols demonstrate improved cell alignment in samples generated with exposure directly at the development temperature, highlighting the relevance of the height enhancement reached by this method. Although cell alignment is well-known to be enhanced by increasing the relief height of the polymeric grating, our work demonstrates nano-second laser interference photoembossing as a powerful tool to easily prepare polymeric gratings with tunable topography in the range of interest for fundamental cell alignment studies

Abundances and Physical Conditions in the Warm Neutral Medium Towards mu Columbae

We present ultraviolet interstellar absorption line measurements for the sightline towards the O9.5 V star mu Columbae obtained with the Goddard High Resolution Spectrograph (GHRS) on board the Hubble Space Telescope. These archival data represent the most complete GHRS interstellar absorption line measurements for any line of sight towards an early-type star. The 3.5 km/s resolution of the instrument allow us to accurately derive the gas-phase column densities of many important ionic species in the diffuse warm neutral medium using a combination of apparent column density and component fitting techniques, and we study in detail the contamination from ionized gas along this sightline. The low-velocity material shows gas-phase abundance patterns similar to the warm cloud (cloud A) towards the disk star zeta Oph, while the component at v = +20.1 km/s shows gas-phase abundances similar to those found in warm halo clouds. We find the velocity-integrated gas-phase abundances of Zn, P, and S relative to H along this sightline are indistinguishable from solar system abundances. We discuss the implications of our gas-phase abundance measurements for the composition of interstellar dust. The relative ionic column density ratios of the intermediate velocity components show the imprint both of elemental incorporation into grains and (photo)ionization. The components at v = -30 and -48 km/s along this sightline likely trace shocked gas with very low hydrogen column densities. Appendices include a new derivation of the GHRS instrumental line spread function, and a new very accurate determination of the total H I column along this sightline. (Abridged)Comment: Accepted for publication in the Astrophysical Journal. 80 pages including 19 embedded figures and 12 embedded tables. Version with higher resolution figures can be downloaded from http://fuse.pha.jhu.edu/~howk/Papers/papers.htm