89 research outputs found
Π€ΠΠ’ΠΠΠΠΠΠΠΠ§ΠΠ‘ΠΠΠ― ΠΠΠΠΠ’ΠΠΠΠ¦ΠΠ― MYCOBACTERIUM TUBERCULOSIS Π ΠΠΠΠ₯ΠΠΠ ΠΠΠΠ IN VITRO
Goal: to detect the best mode of in vitro photodynamic inactivation of M. tuberculosis by Radahlorin.Subjects and methods. The activity of culture of M. tuberculosis, H37Rv strain, photosensitized by 0.00005% Radohlorin was compared and the intensity of growth was assessed after photodynamic inactivation by different doses of light energy with 662 nm wavelength.Results. For the first time, anti-microbial properties of E6 chlorine in the form of medicamental photosensitizer (0.00005% Radohlorin) suppressing museum strain of M. tuberculosis of H37Rv were detected. Photoinactivation of M. tuberculosis depends on the dose and achieves its maximum in 10Β minutes of light exposure with light energy of 0.5 W.Β Π¦Π΅Π»Ρ: Π²ΡΡΠ²ΠΈΡΡ ΠΎΠΏΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΉ ΡΠ΅ΠΆΠΈΠΌ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ (Π€ΠΠ) M. tuberculosis ΡΠ°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½ΠΎΠΌ in vitro.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΡΠ»ΡΡΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ M. tuberculosis H37Rv, ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ°Π΄Π°Ρ
Π»ΠΎ- ΡΠΈΠ½ΠΎΠΌ 0,00005%, Ρ ΠΎΡΠ΅Π½ΠΊΠΎΠΉ ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΎΡΡΠ° ΠΏΠΎΡΠ»Π΅ Π€ΠΠ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ Π΄ΠΎΠ·Π°ΠΌΠΈ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ Ρ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΏΠ΅ΡΠ²ΡΠ΅ Π²ΡΡΠ²Π»Π΅Π½Ρ Π°Π½ΡΠΈΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° Ρ
Π»ΠΎΡΠΈΠ½Π° Π6 Π² Π²ΠΈΠ΄Π΅ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΎΡΠ° (ΡΠ°Π΄Π°Ρ
Π»ΠΎΡΠΈΠ½ 0,00005%) Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΌΡΠ·Π΅ΠΉΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΌΠΌΠ° M. tuberculosis H37Rv. Π€ΠΎΡΠΎΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ Π²ΠΎΠ·Π±ΡΠ΄ΠΈΡΠ΅Π»Ρ Π΄ΠΎΠ·ΠΎΠ·Π°Π²ΠΈΡΠΈΠΌΠ° ΠΈ Π΄ΠΎΡΡΠΈΠ³Π°Π΅Ρ ΠΏΡΠ΅Π΄Π΅Π»ΡΠ½ΡΡ
Π·Π½Π°ΡΠ΅Π½ΠΈΠΉ ΡΠ΅ΡΠ΅Π· 10 ΠΌΠΈΠ½ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΏΡΠΈ ΠΌΠΎΡΠ½ΠΎΡΡΠΈ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ 0,5 ΠΡ.
Π€ΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΡ Mycobacterium tuberculosis ΠΌΠ΅ΡΠΈΠ»Π΅Π½ΠΎΠ²ΡΠΌ ΡΠΈΠ½ΠΈΠΌ in vitro
The objective: to investigate the anti-tuberculosis effect of laser photodynamic inactivation (PDI) of M. tuberculosis H37Rv in vitro by methylene blue (MB) in the minimum concentration (1 ΞΌg/ml) with laser radiation of 662 nm. Subjects and methods. A comparative analysis of the intensity of growth of Mycobacterium tuberculosis H37Rv after laser irradiation and laser FDI by MB with different doses of light energy was carried out. Results. Laser radiation with a wavelength of 662 nm was found to have an inhibitory effect on the growth of M. tuberculosis H37Rv. FDI of Mycobacterium tuberculosis was first registered in the presence of a minimum concentration of MB (1 Β΅g/ml) which suppressed colony growth by 97 and 93% when they were processed by radiation with a wavelength of 662 nm with the lowest density of doses of light energy (46.9 and 93.75 J/cm2).Π¦Π΅Π»Ρ: ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΡΠΈΠ²ΠΎΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π½ΡΡ
ΡΡΡΠ΅ΠΊΡΠΎΠ² Π»Π°Π·Π΅ΡΠ½ΠΎΠΉ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ½Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ (Π€ΠΠ) M. tuberculosis H37Rv in vitro ΠΌΠ΅ΡΠΈΠ»Π΅Π½ΠΎΠ²ΡΠΌ ΡΠΈΠ½ΠΈΠΌ (ΠΠ‘) Π² ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ (1 ΠΌΠΊΠ³/ΠΌΠ») ΠΏΡΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠΌ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠΈ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΡΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΈΠ½ΡΠ΅Π½ΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΎΡΡΠ° Mycobacterium tuberculosis H37Rv ΠΏΠΎΡΠ»Π΅ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±Π»ΡΡΠ΅Π½ΠΈΡ ΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠΉ Π€ΠΠ ΠΠ‘ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π΄ΠΎΠ·Π°Ρ
ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π»Π°Π·Π΅ΡΠ½ΠΎΠ΅ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ ΠΎΠΊΠ°Π·ΡΠ²Π°Π΅Ρ ΠΈΠ½Π³ΠΈΠ±ΠΈΡΡΡΡΠ΅Π΅ Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΡΠΎΡΡΠΎΠ²ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° M. tuberculosis H37Rv. ΠΠΏΠ΅ΡΠ²ΡΠ΅ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½Π° Π€ΠΠ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ ΠΠ‘ (1 ΠΌΠΊΠ³/ΠΌΠ»), ΠΏΡΠΈ ΠΊΠΎΡΠΎΡΠΎΠΉ ΡΠ΅Π°Π»ΠΈΠ·ΡΠ΅ΡΡΡ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠ΅ ΡΠΎΡΡΠ° ΠΊΠΎΠ»ΠΎΠ½ΠΈΠΉ Π½Π° 97 ΠΈ 93% ΠΏΡΠΈ ΠΈΡ
ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ΅ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ Ρ Π½Π°ΠΈΠΌΠ΅Π½ΡΡΠΈΠΌΠΈ Π·Π½Π°ΡΠ΅Π½ΠΈΡΠΌΠΈ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ Π΄ΠΎΠ· ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ (46,9 ΠΈ 93,75 ΠΠΆ/ΡΠΌ2)
ΠΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ Π² ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° Ρ ΠΌΡΡΠ΅ΠΉ
The objective: to study the efficacy of antimicrobial photodynamic therapy (APDT) using hydroxyaluminum phthalocyanine (Photosens) as a photosensitizer in the experimental model of tuberculosis infection in mice.Subjects and Methods. Balb/cmice were infected with the multiple drug resistant (MDR) strain of M. tuberculosis Beijing BO/W148. APDT with hydroxyaluminum phthalocyanine and red laser light exposure were started on Day 45. A total of 4 sessions of APDT on the projection of both lungs, liver and spleen were performed. On Day 60, the therapeutic eff ect of APDTwas assessed by evaluation of severity of mycobacterial load and specific granulomatous infiltration in the lungs, liver and spleen. The absorption of light energy of laser radiation by the chest and abdominal walls, as well as tissues of lungs, liver and spleen was determined by photometry using afluovisor.Results. The tissues of the experimental animal retain a significant amount of light energy, however, the residual value of light transmission is sufficient to provide a pronounced therapeutic effect which manifests itself as a significant decrease of mycobacterial load and specific inflammatory process in all the studied internal organs.Conclusion. APDT may be an effective tool to treat some forms of tuberculous infection including those caused by M. tuberculosis with MDR.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΈΠ·ΡΡΠΈΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ Π°Π½ΡΠΈΠΌΠΈΠΊΡΠΎΠ±Π½ΠΎΠΉ ΡΠΎΡΠΎΠ΄ΠΈΠ½Π°ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅ΡΠ°ΠΏΠΈΠΈ (ΠΠ€ΠΠ’) Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠΎΡΠΎΡΠ΅Π½ΡΠΈΠ±ΠΈΠ»ΠΈΠ·Π°ΡΠΎΡΠ° ΡΡΠ°Π»ΠΎΡΠΈΠ½Π°Π½Π° Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ°Π»ΡΠΌΠΈΠ½ΠΈΡ (Π€ΠΎΡΠΎΡΠ΅Π½Ρ) Π² ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ ΠΌΠΎΠ΄Π΅Π»ΠΈ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ Ρ ΠΌΡΡΠ΅ΠΉ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠΈ Π»ΠΈΠ½ΠΈΠΈ Balb/c ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ ΡΡΠ°ΠΌΠΌΠΎΠΌ M. tuberculosis Ρ ΠΌΠ½ΠΎΠΆΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ Π»Π΅ΠΊΠ°ΡΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΡΡ (ΠΠΠ£) Beij ing BO/W148. ΠΠ€ΠΠ’ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΡΠ°Π»ΠΎΡΠΈΠ°Π½ΠΈΠ½Π° Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ°Π»ΡΠΌΠΈΠ½ΠΈΡ ΠΈ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΊΡΠ°ΡΠ½ΡΠΌ Π»Π°Π·Π΅ΡΠΎΠΌ Π½Π°ΡΠΈΠ½Π°Π»ΠΈ Π½Π° 45 ΡΡΡΠΊΠΈ. ΠΡΠ΅Π³ΠΎ Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ 4 ΡΠ΅Π°Π½ΡΠ° ΠΠ€ΠΠ’ Π½Π° ΠΏΡΠΎΠ΅ΠΊΡΠΈΠΈ ΠΎΠ±ΠΎΠΈΡ
Π»Π΅Π³ΠΊΠΈΡ
, ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈ ΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠΈ. Π’Π΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΡΠ΅ΠΊΡ ΠΠ€ΠΠ’ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ Π½Π° 60-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠΉ Π³ΡΠ°Π½ΡΠ»Π΅ΠΌΠ°ΡΠΎΠ·Π½ΠΎΠΉ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΈ Π² Π»Π΅Π³ΠΊΠΈΡ
, ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈ ΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠ΅. ΠΠ±ΡΠΎΡΠ±ΡΠΈΡ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ Π³ΡΡΠ΄Π½ΠΎΠΉ ΠΈ Π±ΡΡΡΠ½ΠΎΠΉ ΡΡΠ΅Π½ΠΊΠΎΠΉ, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠΊΠ°Π½ΡΠΌΠΈ Π»Π΅Π³ΠΊΠΈΡ
, ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈ ΡΠ΅Π»Π΅Π·Π΅Π½ΠΊΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ»ΡΠΎΠ²ΠΈΠ·ΠΎΡΠ°.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π’ΠΊΠ°Π½ΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΆΠΈΠ²ΠΎΡΠ½ΠΎΠ³ΠΎ Π·Π°Π΄Π΅ΡΠΆΠΈΠ²Π°ΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠΉ ΡΠ½Π΅ΡΠ³ΠΈΠΈ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΎΡΡΠ°ΡΠΎΡΠ½Π°Ρ Π²Π΅Π»ΠΈΡΠΈΠ½Π° ΡΠ²Π΅ΡΠΎΠΏΡΠΎΠΏΡΡΠΊΠ°Π½ΠΈΡ Π΄ΠΎΡΡΠ°ΡΠΎΡΠ½Π° Π΄Π»Ρ ΠΎΠΊΠ°Π·Π°Π½ΠΈΡ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΡΠ΅ΠΊΡΠ°, ΠΏΡΠΎΡΠ²Π»ΡΠ²ΡΠ΅Π³ΠΎΡΡ Π² Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠΈ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° Π²ΠΎ Π²ΡΠ΅Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
Π²Π½ΡΡΡΠ΅Π½Π½ΠΈΡ
ΠΎΡΠ³Π°Π½Π°Ρ
.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΠ€ΠΠ’ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌ ΡΠΏΠΎΡΠΎΠ±ΠΎΠΌ Π»Π΅ΡΠ΅Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠΎΡΠΌ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π²ΡΠ·Π²Π°Π½Π½ΡΡ
M. tuberculosis Ρ ΠΠΠ£
Strategies for Abatement of Contamination of Tableted Live Plague Vaccine at Different Stages of Manufacturing
Nowadays, problem of tableted drug form contamination with extraneous micro-flora is in the spotlight of scientists, as the specific share of these medical preparations in the world market amounts to more than 60 % and has a tendency to increase. Thus, objective of the study is to investigate the degree of contamination of the basic and auxiliary raw materials at different stages of live plague vaccine manufacturing, rapid dissolving tablets, and the ways to reduce it. Materials and methods. Utilized has been lyophilized Y. pestis live culture of the vaccine strain EV NIIEG, and the live plague vaccine, rapid dissolving tablets. Carried out has been assessment of βmicrobiological purityβ at different stages of tableted live plague vaccine manufacturing: grinding, mixing, granulation, sublimation, and palletizing. Results and conclusions. Identified is the dynamic pattern of quantitative micro-flora composition of the mentioned above drug. Established is the alteration of microbial impurity at separate technological manufacturing steps. Specified is the technological stage with the most expressed contamination. Analysis of factors, which affect vaccine impurity, has revealed that finished dosage-form quality improvement is impossible without incoming control of stock and auxiliary materials, as well as enhancement of manufacturing procedure up to the level, complying with applicable pharmaceutical production standards. It is experimentally proved that series of tableted live plague vaccine, obtained using modernized technological equipment, provide for 3-fold reduction of contamination
ΠΠΠΠ―ΠΠΠ ΠΠΠΠΠ ΠΠΠΠ ΠΠΠΠ£Π§ΠΠΠΠ― ΠΠΠΠΠΠ ΠΠΠΠΠ« 662Β ΠΠ ΠΠ Π ΠΠ‘Π’ MYCOBACTERIUM TUBERCULOSIS INΒ VITRO
Goal of the study: to define the effect of various doses of laser radiation with 662 nm wave on the growth of M. tuberculosis in vitro.Materials and methods. Samples of mycobacterial suspension of M. tuberculosis H37Rv were processed by monopositional light radiation (Ξ» =Β 662Β nm) in six dosing regimens varying in power and duration of the exposure to the light. All samples of mycobacterial suspension of M. tuberculosis were inoculated on the solid nutritional media of Lowenstein-Jensen in triplets for each dose of the exposure to light. Cultures were incubated under 37Β°Π‘ for 90 days with weekly inspection of samples.Results. Continuous irradiation by diffused laser with 662 nm wave provides the most expressed bacteriostatic and bactericidal effects against M.Β tuberculosis H37Rv under the density of the energy dose of 234.5 and 703.5 of J/sq.cm. Such a dose was obtained through 5 and 15-minute exposure respectively.Β Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ in vitro Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π΄ΠΎΠ· Π»Π°Π·Π΅ΡΠ½ΠΎΠ³ΠΎ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΡ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ Π½Π° ΡΠΎΡΡΠΎΠ²ΡΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° M.Β tuberculosis.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ Π½Π° ΠΎΠ±ΡΠ°Π·ΡΡ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π²Π·Π²Π΅ΡΠΈ M. tuberculosis H37Rv ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΈ Π½Π΅ΠΏΡΠ΅ΡΡΠ²Π½ΡΠΌ ΠΌΠΎΠ½ΠΎΠΏΠΎΠ·ΠΈΡΠΈΠΎΠ½Π½ΡΠΌ ΡΠ²Π΅ΡΠΎΠ²ΡΠΌ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ΠΌ (Ξ» = 662 Π½ΠΌ) Π² ΡΠ΅ΡΡΠΈ ΡΠ΅ΠΆΠΈΠΌΠ°Ρ
Π΄ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ, Π·Π°Π²ΠΈΡΡΡΠΈΡ
ΠΎΡ ΠΌΠΎΡΠ½ΠΎΡΡΠΈ ΠΈ Π΄Π»ΠΈΡΠ΅Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. ΠΠ½ΠΎΠΊΡΠ»ΡΡΠΈΡ Π²ΡΠ΅Ρ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² ΡΡΡΠΏΠ΅Π½Π·ΠΈΠΉ ΠΌΠΈΠΊΠΎΠ±Π°ΠΊΡΠ΅ΡΠΈΠΉ ΡΡΠ±Π΅ΡΠΊΡΠ»Π΅Π·Π° ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»Π°ΡΡ Π½Π° ΠΏΠ»ΠΎΡΠ½ΡΠ΅ ΠΏΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΡΠ΅ ΡΡΠ΅Π΄Ρ ΠΠ΅Π²Π΅Π½ΡΡΠ΅ΠΉΠ½Π° β ΠΠ΅Π½ΡΠ΅Π½Π° Π² ΡΡΠΈΠΏΠ»Π΅ΡΠ°Ρ
Π΄Π»Ρ ΠΊΠ°ΠΆΠ΄ΠΎΠΉ Π΄ΠΎΠ·Ρ ΡΠ²Π΅ΡΠΎΠ²ΠΎΠ³ΠΎ Π²ΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. ΠΠ½ΠΊΡΠ±Π°ΡΠΈΡ ΠΏΠΎΡΠ΅Π²ΠΎΠ² ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»Π°ΡΡ ΠΏΡΠΈ 37Β°Π‘ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 90 Π΄Π½Π΅ΠΉ Ρ Π΅ΠΆΠ΅Π½Π΅Π΄Π΅Π»ΡΠ½ΡΠΌ ΠΏΠ΅ΡΠ΅ΡΠΌΠΎΡΡΠΎΠΌ ΠΎΠ±ΡΠ°Π·ΡΠΎΠ².Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ΅ΠΏΡΠ΅ΡΡΠ²Π½ΠΎΠ΅ ΠΈΠ·Π»ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΠΎΠ»ΡΠΏΡΠΎΠ²ΠΎΠ΄Π½ΠΈΠΊΠΎΠ²ΠΎΠ³ΠΎ Π»Π°Π·Π΅ΡΠ° Ρ Π΄Π»ΠΈΠ½ΠΎΠΉ Π²ΠΎΠ»Π½Ρ 662 Π½ΠΌ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΌΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΈ Π±Π°ΠΊΡΠ΅ΡΠΈΡΠΈΠ΄Π½ΡΠΌΠΈ ΡΡΡΠ΅ΠΊΡΠ°ΠΌΠΈ Π² ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ M. tuberculosis H37Rv ΠΏΡΠΈ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ Π΄ΠΎΠ·Ρ ΡΠ½Π΅ΡΠ³ΠΈΠΈ 234,5 ΠΈ 703,5 ΠΠΆ/ΡΠΌ2 . Π’Π°ΠΊΠ°Ρ Π΄ΠΎΠ·Π° Π±ΡΠ»Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΏΡΠΈ 5- ΠΈ 15-ΠΌΠΈΠ½ΡΡΠ½ΠΎΠΉ ΡΠΊΡΠΏΠΎΠ·ΠΈΡΠΈΠΈ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ.
Enantioselective component selection in multicomponent supramolecular gels
We investigate a two-component acid-amine gelation system in which chirality plays a vital role. A carboxylic acid based on a second generation l-lysine dendron interacts with chiral amines and subsequently assembles into supramolecular gel fibers. The chirality of the amine controls the assembly of the resulting diastereomeric complexes, even if this chirality is relatively "poor quality". Importantly, the selective incorporation of one enantiomer of an amine over the other into the gel network has been demonstrated, with the R amine that forms complexes which assemble into the most stable gel being primarily selected for incorporation. Thermodynamic control has been proven by forming a gel exclusively with an S amine, allowing the R enantiomer to diffuse through the gel network, and displacing it from the "solidlike" fibers, demonstrating that these gels adapt and evolve in response to chemical stimuli to which they are exposed. Excess amine, which remains unincorporated within the solidlike gel fiber network, can diffuse out and be reacted with an isocyanate, allowing us to quantify the enantioselectivity of component selection but also demonstrating how gels can act as selective reservoirs of potential reagents, releasing them on demand to undergo further reactions; hence, component-selective gel assembly can be coupled with controlled reactivity
Polymorphism: an evaluation of the potential risk to the quality of drug products from the FarmΓ‘cia Popular Rede PrΓ³pria
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