Framework for opinion as a service on review data of customer using semantics based analytics

At Opinion mining plays a significant role in representing the original and unbiased perception of the products/services. However, there are various challenges associated with performing an effective opinion mining in the present era of distributed computing system with dynamic behaviour of users. Existing approaches is more laborious towards extracting knowledge from the reviews of user which is further subjected to various rounds of operation with complex procedures. The proposed system addresses the problem by introducing a novel framework called as Opinion-as-a-Service which is meant for direct utilization of the extracted knowledge in most user friendly manner. The proposed system introduces a set of three sequential algorithm that performs aggregated of incoming stream of opinion data, performing indexing, followed by applying semantics for extracting knowledge. The study outcome shows that proposed system is better than existing system in mining performance

Leber's Hereditary Optic Neuropathy-Gene Therapy: From Benchtop to Bedside

Leber's hereditary optic neuropathy (LHON) is a maternally transmitted disorder caused by point mutations in mitochondrial DNA (mtDNA). Most cases are due to mutations in genes encoding subunits of the NADH-ubiquinone oxidoreductase that is Complex I of the electron transport chain (ETC). These mutations are located at nucleotide positions 3460, 11778, or 14484 in the mitochondrial genome. The disease is characterized by apoplectic, bilateral, and severe visual loss. While the mutated mtDNA impairs generation of ATP by all mitochondria, there is only a selective loss of retinal ganglion cells and degeneration of optic nerve axons. Thus, blindness is typically permanent. Half of the men and 10% of females who harbor the pathogenic mtDNA mutation actually develop the phenotype. This incomplete penetrance and gender bias is not fully understood. Additional mitochondrial and/or nuclear genetic factors may modulate the phenotypic expression of LHON. In a population-based study, the mtDNA background of haplogroup J was associated with an inverse relationship of low-ATP generation and increased production of reactive oxygen species (ROS). Effective therapy for LHON has been elusive. In this paper, we describe the findings of pertinent published studies and discuss the controversies of potential strategies to ameliorate the disease

An Enhanced Spatio-Temporal Human Detected Keyframe Extraction

Due to the immense availability of Closed-Circuit Television surveillance, it is quite difficult for crime investigation due to its huge storage and complex background. Content-based video retrieval is an excellent method to identify the best Keyframes from these surveillance videos. As the crime surveillance reports numerous action scenes, the existing keyframe extraction is not exemplary. At this point, the Spatio-temporal Histogram of Oriented Gradients - Support Vector Machine feature method with the combination of Background Subtraction is appended over the recovered crime video to highlight the human presence in surveillance frames. Additionally, the Visual Geometry Group trains these frames for the classification report of human-detected frames. These detected frames are processed to extract the keyframe by manipulating an inter-frame difference with its threshold value to favor the requisite human-detected keyframes. Thus, the experimental results of HOG-SVM illustrate a compression ratio of 98.54%, which is preferable to the proposed work\u27s compression ratio of 98.71%, which supports the criminal investigation

Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag

<p>Abstract</p> <p>Background</p> <p>The ESCRT (endosomal sorting complex required for transport) machinery functions to sort cellular receptors into the lumen of the multivesicular body (MVB) prior to lysosomal degradation. ESCRT components can also be recruited by enveloped viruses to sites of viral assembly where they have been proposed to mediate viral egress. For example, HIV-1 budding is dependent on Gag-mediated recruitment of the cellular ESCRTs-I, -III, AIP1/Alix and Vps4 proteins. Viral recruitment of ESCRT proteins could therefore impact on host cell processes such as receptor downregulation.</p> <p>Results</p> <p>Here we show that downregulation of the HIV-1 co-receptor, CXCR4, by its ligand SDF-1, is ESCRT-I dependent. Expression of HIV-1 Gag attenuated downregulation of CXCR4, resulting in accumulation of undegraded receptors within intracellular compartments. The effect of Gag was dependent on an ESCRT-I interacting motif within the C-terminal p6 region of Gag. In contrast, PMA-induced downregulation of the HIV-1 receptor CD4 was independent of ESCRT-I and Vps4; HIV-1 Gag had no effect on this process.</p> <p>Conclusion</p> <p>These results establish that the HIV-1 receptor, CD4, and co-receptor, CXCR4 are differentially regulated by ESCRT proteins. HIV-1 Gag selectively modulates protein sorting at the MVB, interfering with ESCRT-I dependent but not ESCRT-I independent processes.</p

Analysis of incidence of caesarean sections in primigravida

Background: Current study was conducted to determine the incidence of caesarean sections in primigravida.Methods: A total number of 1698 primigravidas were admitted in the department of DNB district hospital Ballari, out of which 501 primigravidas who underwent caesarean section were included in the study.Results: Caesarean section rate in primigravida was observed to be 29.5%, 55% of women were from rural areas and 45% from urban areas, 69.9% women belonged to lower class 31.1% belonged to upper middle class, 25% were illiterate while 75% were literate.Conclusions: Meta-analysis suggests that the occurrence of pregnancy complications differ according to fetal sex with a higher cardiovascular and metabolic load for the mother in the presence of a male fetus.

Effect of Clerodendrum serratum leaf extract on biochemical and oxidative stress parameters of testis in 7, 12-dimethylbenz[a]anthracene induced skin carcinogenesis in Swiss albino mice

The biochemical contents and antioxidant potential of Clerodendrum serratum (Verbenaceae) leaf extract (CSLE) on 7, 12-dimethylbenz[a]anthracene (DMBA) induced skin carcinogenicity in testis of mice was investigated. Group I received distilled water served as control. The skin lesions were induced by twice-weekly topical application of DMBA for 2 weeks on the shaved backs of group II, III, IV and V mice. CSLE was administered to group III, IV and V mice at the dose of 300, 600 and 900 mg/kg b.wt/day, for 4 week before DMBA application, and continued till 45 days. On 46th day the mice were sacrificed, testis were dissected out freed from adherent tissue and weighed to nearest milligram and evaluated the biochemical contents DNA, RNA, protein, glycogen, cholesterol, lactate dehydrogenase (LDH), Succinic dehydrogenase (SDH), acid phosphatase (ACP) and alkaline phosphatase (AKP) activities, oxidative stress parameters, levels of glutathione (GSH), thiobarbaturic acid reactive substances (TBARS), superoxide dismutase (SOD), catalase (CAT) and glutathione-s-transferase (GST). DMBA induced skin carcinogenesis decreased body and testis weight, DNA, RNA, protein, glycogen, GSH level, SDH, AKP, SOD, CAT and GST activities. But there was increase in cholesterol content, LDH, ACP activities and TBARS level. DMBA act via generating reactive oxygen species (ROS) as tumor initiator and free radicals inducing oxidative stress. The results revealed that there was a recovery in biochemical contents, dehydrogenases, phosphatases and oxidative stress parameters in testis. Thus, the present study inferred that CSLE administration significantly curtailed tumor development and counteracted all the biochemical effects. Many plant secondary metabolites exhibit potent anticarcinogenic potential and known to exert their effects by quenching reactive oxygen, inhibiting lipid peroxidation

IS DOSE TITRATION REQUIRED FOR ANTIHYPERTENSIVE AGENTS IN GERIATRIC DIABETIC PATIENTS?

Objective: The objective of this study is to evaluate the antihypertensive drug usage and dosage differences between geriatric and non-geriatric diabetics with reference to the duration of hypertension and creatinine clearance (Crcl).Methods: In this observational study, patients with type 2 diabetes mellitus were grouped into geriatric (age ≥60 years) and non-geriatric (age &lt;60 years). Patients' demographic data, duration of hypertension, drugs prescribed, and serum creatinine were recorded after the patients had a stabilized antihypertensive dose for 6 months. Crcl was calculated using Cockcroft–Gault formula. The dosages of antihypertensives were converted into equivalent doses for easy comparison within a group. For angiotensin-converting enzyme inhibitors (ACEIs), enalapril was considered as prototype, and for angiotensin receptor blockers (ARBs) losartan, beta-blocker atenolol, and calcium channel blockers (CCBs), amlodipine was considered as prototype. Univariate analysis was done for comparison of drug doses between groups.Results: A total of 336 diabetics with hypertension were included, of which 252 were geriatric and 84 non-geriatric. Duration of hypertension was expectedly longer in the geriatric group (8.40±7.26 vs. 5.46±5.67; p=0.001). Systolic blood pressure was higher in geriatrics (137.14±13.51 vs. 133.38±12.49; p=0.01). When adjusted for the duration of hypertension and Crcl, there were no significant differences in the mean converted equivalent doses of beta-blockers, CCBs, ARBs, and hydrochlorothiazide between geriatrics and non-geriatrics. However, statistically significant lower converted equivalent doses of all ACEIs were needed in geriatrics compared to non-geriatrics, when adjusted for duration of hypertension and Crcl. Enalapril required 20.57% and ramipril required 18.36% dose reduction in geriatrics compared to non-geriatrics.Conclusion: A 20% dosage reduction is needed for ACEIs in the elderly

Geospatial Technologies in the extraction of Groundwater Potential Zones

The utmost source which is contributing to the supply of water to the society is groundwater. Depletion of undergroundwater is enormously caused by eruptive growing rate of human population along with needs in the form of industrialand urbanized growth, indigent practices in irrigation methods and deforestation. As surface fresh water is very limit, waterdemand is drastically increasing for the needs. In this connection, there is immense predominant for the natural sourcecalled groundwater. It is mandatory for the communities not only to targeting the resource called groundwater but also toprovide remedial measures to replenishment the groundwater. Enormous investigations are in the process globally, to meetthe requirement to compensate resource for the needs in all aspects. Geospatial techniques are playing vital role in theextraction of groundwater resources by means of spatial and temporal data variations along with its integration analysis inthe form of separable layers to derive the solutions. In this connection, Nandyal mandal of Kurnool is selected to demarcatethe potential zones of groundwater by using this geospatial technology.The main motto of the research work is to identifythe effective potential groundwater zones by applying methodsand integration techniques of Geographic InformationSystem and remote sensing. This gives more information for the planning and management of the ground water.Byemploying geospatial technologies, the integrated composite output for potential zones is demarcated with help of keyparameters such as drainage, lineament, slope, geology, geomorphology, land use land cover and existing groundwaterlevels. All these thematic layers are extracted by using the satellite data and other available sources by using remote sensingand GIS. All these themes are demarcated using basic elements to identify the respective classes. Proper weightages areassignedtoeachclassofallthemesintheformofseparatecategorybasedonimportanceofweightagefromexcellenttopoor based on suitability to avail the groundwater sources. This process is applied for the considered themes and isreclassified based on results. It is then integrated with weighted overlay operation in ARCGIS environment. Appropriateweightage percentages which are equal to 100%, are given prior to overlay analysis of hydrology tools of ArcGIS. Variouszones like excellent, very good, good, moderate, poor are categorized for the integrated potential zones of groundwatersource. The use of suggested methodology is applied and demonstrated for a selected case study area in Nandyal Mandal ofKurnool District of Andhra Pradesh, India. Integrated output layout will be effectively useful in the demarcation of potentialresource zones. This demarcation area zone system is not only to identify the zones but also helpful in the replenishing theresources of the study area. The digital elevation model is also used to extract slope and drainage themes of the study area.The groundwater potential zones were obtained by overlaying all the thematic maps in terms of weighted overlay analysismethod using the spatial analysis tool in ArcGIS 10.1. This ground water potential information will be useful for effectiveidentificationofsuitablelocationsfor extractionofsuchgroundwater

Improved Serological Techniques for the Detection and Identification of Groundnut Viruses

Serology is indispensable for the detection and identification of plant viruses. Recently, the highly sensitive enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) techniques have been developed and may replace some of the conventional serological methods. The direct or standard double-antibody sandwich (DAS) form of ELISA. as first described by Clark and Adams (1977), has wide applications in plant virology. An indirect form of ELISA (I-ELISA) has recently been developed by Barbara and Clark (1982). The ISEM procedure developed by Derrick (1973) combines the specificity of serology with coventional electron microscopy. DAS-ELISA, I-ELISA and ISEM have been adapted for the detection and identification of several groundnut viruses. This paper will provide a description of each technique and its application for the detection and characterization of viruses occurring on groundnut in India