Detection of human neutrophil elastase (HNE) on wound dressings as marker of inflammation

Chronic wound fluids have elevated concentration of human neutrophil elastase (HNE) which can be used as inflammation/infection marker. Our goal is to develop functional materials for fast diagnosis of wound inflammation/infection by using HNE as a specific marker. For that, fluorogenic peptides with a HNE-specific cleavage sequence were incorporated into traditional textile dressings, to allow real-time detection of the wound status. Two different fluorogenic approaches were studied in terms of intensity of the signal generated upon HNE addition: a fluorophore 7-amino-4-trifluormethylcoumarin (AFC) conjugated to a HNE-specific peptide and two fluorophore/quencher pairs (FAM/Dabcyl and EDANS/Dabcyl) coupled to a similar peptide as a Förster resonance energy transfer (FRET) strategy. Also, two immobilization methods were tested: sonochemistry immobilization onto a cotton bandage and glutaraldehyde (GTA)-assisted chemical crosslinking onto a polyamide dressing. The immobilized fluorogenic AFC peptide showed an intense fluorescence emission in the presence of HNE. HNE also induced an enhanced fluorescent signal with the EDANS/Dabcyl FRET peptide which showed to be a more sensitive and effective strategy than the AFC peptide. However, its chemical immobilization onto the polyamide dressing greatly decreased its detection, mainly due to the more difficult access of the enzyme to the cleavage sequence of the immobilized peptide. After optimization of the in situ immobilization, it will be possible to use these fluorescence-functionalized dressings for an effective and specific monitoring of chronic wounds by simply using a portable ultraviolet (UV) light source. We envision that the development of this point-of-care medical device for wound control will have a great impact on patients life quality and reduction of costs on health care system.This study was funded by the European project InFact-Functional materials for fast diagnosis of wound infection (FP7-NMP-2013-SME-7-grant agreement no. 604278). The work done at Centre of Biological Engineering (CEB) was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte 2020-Programa Operacional Regional do Norte

Evaluation of an evaporation suppressing monolayer system in a controlled wave tank environment: a pilot investigation

Due to long-term drought conditions coupled with the apparent influence of global warming, compounding water loss has been a very serious issue across the vast majority of the Australian continent. During these drought conditions, the evaporative effect outweighs the amount of precipitation being received on a year to year basis. Several methods have been introduced in recent history to inhibit the amount of evaporative loss from various types of water bodies such as the application of thin layer chemical films (monolayers). A series of solvent, solid and suspension derived prototype monolayers, based on ethylene glycol monooctadecyl ether (C18E1), are examined in this current study as an approach to eliminate the problems seen to occur with the previous types of monolayers. This research evaluates the fundamental effect of wind and wave based activity upon these prototype monolayers in an atmospherically controlled enclosure positioned over a large extended water tank using real-time environmental measurements. Selected performance results for the prototype monolayers as measured within the enclosed water tank were compared to results measured from a control monolayer film based on a commonly used octadecanol suspension film. The results show that under varying wind and wave conditions the prototype monolayers inhibit evaporation at a level similar to or better than the octadecanol standard, even when delivered at lower raw dosages