Village Baseline Study: Site Analysis Report for Kaffrine – Kaffrine, Senegal

The village baseline study of Toune Mosquée village in the CCAFS benchmark site Kaffrine in Senegal took place from June 2 to 4, 2011. Natural resources in the village are in a progressive state of degradation. The ‘protected’ forest has almost disappeared, the soils have low fertility and are degraded, and crop production is not sufficient to meet the food needs of a family throughout the year. Families must buy food to fill the gap in production. For that they harvest and sell forest products, which creates a vicious cycle of resource degradation. The male and female groups identified 35 organisations operating in the village including informal groups, state services, associations, NGOs and Muslim brotherhoods. While 18 of those work on food security issues, only 4 are involved in the management and protection of natural resources. Very few agricultural extension and training opportunities target women despite the women’s significant role in agriculture and livestock production. Women obtain information on livestock feeding techniques from people and organisations such as the horticultural project, women’s associations, and water and forest services. Men get information on soil inputs and soil fertility management from other farmers, organisations, radio and television, and from community leaders, notably the office of the village chief and the mosque on Fridays. The radio is the form of media most commonly used by the women but few women own a radio

Village Baseline Study: Site Analysis Report for Yatenga – Tougou, Burkina Faso

The village baseline study of Kononga village in the CCAFS site Yatenga-Tougou in Burkina Faso took place from 19th to 21st July 2011. Focus group discussions were conducted separately for men and women. Male and female participants believe that the natural resources in the village are deteriorating due to population increase and labour shortages related to the exodus of young people to gold mining sites. The village’s vision of the future includes more productive and fertile farmland, a denser forest, deeper and wider water reservoirs, and more boreholes. The men identified 21 organisations in the village, including 9 operating at the community level, while women identified 17 organisations, 10 of which operate at the community level. Men are considered the most important personal source of information in the village. The regional directorate for agriculture, hydrology and fishery resources (DRAHRA) is the single most relevant institutional source of climate and weather information for both male and female participants. Men and women gather information from outside the village via radio broadcasting and particularly Radio “Voix du paysan” (Voice of the Farmer). The market is the most important channel of information access for women. Women have a substantial role in agriculture and livestock production, as well as natural resource management but they have limited access to land or to improved technology or equipment, and few training opportunities

A Spatial Analysis of Rift Valley Fever Virus Seropositivity in Domestic Ruminants in Tanzania

Rift Valley fever (RVF) is an acute arthropod-borne viral zoonotic disease primarily occurring in Africa. Since RVF-like disease was reported in Tanzania in 1930, outbreaks of the disease have been reported mainly from the eastern ecosystem of the Great Rift Valley. This cross-sectional study was carried out to describe the variation in RVF virus (RVFV) seropositivity in domestic ruminants between selected villages in the eastern and western Rift Valley ecosystems in Tanzania, and identify potential risk factors. Three study villages were purposively selected from each of the two Rift Valley ecosystems. Serum samples from randomly selected domestic ruminants (n = 1,435) were tested for the presence of specific immunoglobulin G (IgG) and M (IgM), using RVF enzyme-linked immunosorbent assay methods. Mixed effects logistic regression modelling was used to investigate the association between potential risk factors and RVFV seropositivity. The overall RVFV seroprevalence (n = 1,435) in domestic ruminants was 25.8% and species specific seroprevalence was 29.7%, 27.7% and 22.0% in sheep (n = 148), cattle (n = 756) and goats (n = 531), respectively. The odds of seropositivity were significantly higher in animals sampled from the villages in the eastern than those in the western Rift Valley ecosystem (OR = 1.88, CI: 1.41, 2.51; p<0.001), in animals sampled from villages with soils of good than those with soils of poor water holding capacity (OR = 1.97; 95% CI: 1.58, 3.02; p< 0.001), and in animals which had been introduced than in animals born within the herd (OR = 5.08, CI: 2.74, 9.44; p< 0.001). Compared with animals aged 1-2 years, those aged 3 and 4-5 years had 3.40 (CI: 2.49, 4.64; p< 0.001) and 3.31 (CI: 2.27, 4.82, p< 0.001) times the odds of seropositivity. The findings confirm exposure to RVFV in all the study villages, but with a higher prevalence in the study villages from the eastern Rift Valley ecosystem

Les Espèces vègètales de couverture du sol destinées à la culture du coton en semis direct.

bitstream/item/142514/1/Les-especes-vegetables-de-couverture-du-sol....pdfCOTON-4

Transcriptomic signatures differentiate survival from fatal outcomes in humans infected with Ebola virus

Background In 2014, Western Africa experienced an unanticipated explosion of Ebola virus infections. What distinguishes fatal from non-fatal outcomes remains largely unknown, yet is key to optimising personalised treatment strategies. We used transcriptome data for peripheral blood taken from infected and convalescent recovering patients to identify early stage host factors that are associated with acute illness and those that differentiate patient survival from fatality. Results The data demonstrate that individuals who succumbed to the disease show stronger upregulation of interferon signalling and acute phase responses compared to survivors during the acute phase of infection. Particularly notable is the strong upregulation of albumin and fibrinogen genes, which suggest significant liver pathology. Cell subtype prediction using messenger RNA expression patterns indicated that NK-cell populations increase in patients who survive infection. By selecting genes whose expression properties discriminated between fatal cases and survivors, we identify a small panel of responding genes that act as strong predictors of patient outcome, independent of viral load. Conclusions Transcriptomic analysis of the host response to pathogen infection using blood samples taken during an outbreak situation can provide multiple levels of information on both disease state and mechanisms of pathogenesis. Host biomarkers were identified that provide high predictive value under conditions where other predictors, such as viral load, are poor prognostic indicators. The data suggested that rapid analysis of the host response to infection in an outbreak situation can provide valuable information to guide an understanding of disease outcome and mechanisms of disease

Developpement des systemes de production innovants d’association mais/legumineuses dans la zone subhumide du Mali

Parmi les 3 piliers de l’intégration agriculture-élevage (traction animale, fumure organique, cultures fourragères) diffusés en Afrique de l’Ouest, seules les cultures fourragères ont été peu adoptées. Pourtant, les associations maïs/légumineuses ont l’avantage d’améliorer la production et l’alimentation animale. Elles représentent une alternative dans la gestion des risques et des incertitudes pour les agro-éleveurs, confrontés aux changements globaux (climatiques, pression foncière, dégradation des ressources naturelles). L’objectif de cette étude est de déterminer la contribution des associations maïs/légumineuses dans l’amélioration de la production de maïs grain et le bilan fourrager des exploitations agricoles. Le dispositif en blocs dispersés chez 10 producteurs, a consisté en 3 traitements, T1 (maïs seul), T2 (maïs/ niébé) et T3 (maïs/mucuna) durant 2 campagnes agricoles. En 2013 - 2014, les rendements de maïs grain ont été de 2433 kg/ha et en biomasse 2597 kgMS/ha. Par contre, en 2014 - 2015 les rendements ont été de 1932 kg/ha de maïs grain et de 5134 kgMS/ha de biomasse. Les besoins en matière sèche pour 90 jours de supplémentation ont été de 4,62 UBT en 2013 - 2014 et de 9,13 UBT en 2014 - 2015. Ces résultats montrent la contribution des associations maïs/légumineuses dans l’amélioration du rendement de maïs grain et de fourrages dans la zone subhumide au Mali.Mots clés : légumineuses, maïs, fourrage, Mal

Syndromic approach to arboviral diagnostics for global travelers as a basis for infectious disease surveillance

Background Arboviruses have overlapping geographical distributions and can cause symptoms that coincide with more common infections. Therefore, arbovirus infections are often neglected by travel diagnostics. Here, we assessed the potential of syndrome-based approaches for diagnosis and surveillance of neglected arboviral diseases in returning travelers. Method To map the patients high at risk of missed clinical arboviral infections we compared the quantity of all arboviral diagnostic requests by physicians in the Netherlands, from 2009 through 2013, with a literature-based assessment of the travelers’ likely exposure to an arbovirus. Results 2153 patients, with travel and clinical history were evaluated. The diagnostic assay for dengue virus (DENV) was the most commonly requested (86%). Of travelers returning from Southeast Asia with symptoms compatible with chikungunya virus (CHIKV), only 55% were tested. For travelers in Europe, arbovirus diagnostics were rarely requested. Over all, diagnostics for most arboviruses were requested only on severe clinical presentation. Conclusion Travel destination and syndrome were used inconsistently for triage of diagnostics, likely resulting in vast under-diagnosis of arboviral infections of public health significance. This study shows the need for more awareness among physicians and standardization of syndromic diagnostic algorithm

The Diagnostic Utility of Induced Sputum Microscopy and Culture in Childhood Pneumonia.

BACKGROUND.: Sputum microscopy and culture are commonly used for diagnosing the cause of pneumonia in adults but are rarely performed in children due to difficulties in obtaining specimens. Induced sputum is occasionally used to investigate lower respiratory infections in children but has not been widely used in pneumonia etiology studies. METHODS.: We evaluated the diagnostic utility of induced sputum microscopy and culture in patients enrolled in the Pneumonia Etiology Research for Child Health (PERCH) study, a large study of community-acquired pneumonia in children aged 1-59 months. Comparisons were made between induced sputum samples from hospitalized children with radiographically confirmed pneumonia and children categorized as nonpneumonia (due to the absence of prespecified clinical and laboratory signs and absence of infiltrate on chest radiograph). RESULTS.: One induced sputum sample was available for analysis from 3772 (89.1%) of 4232 suspected pneumonia cases enrolled in PERCH. Of these, sputum from 2608 (69.1%) met the quality criterion of <10 squamous epithelial cells per low-power field, and 1162 (44.6%) had radiographic pneumonia. Induced sputum microscopy and culture results were not associated with radiographic pneumonia, regardless of prior antibiotic use, stratification by specific bacteria, or interpretative criteria used. CONCLUSIONS.: The findings of this study do not support the culture of induced sputum specimens as a diagnostic tool for pneumonia in young children as part of routine clinical practice

Pattern Analysis of Sorghum Genotype x Environment Interaction for Leaf, Panicle, and Grain Anthracnose in Mali

Resistance to anthracnose, caused by Colletotrichum gramUiicola, in.sorghum was identified through field screening at two locations (Samanko and Longorofc) in Mali. The occurrence and progress of anthracnose were monitored on 19 sorghum lines plus resistant and susceptible checks in the 1996 to 1998 rainy seasons. Foliar anthracnose severity was assessed at regular intervals throughout the season. Area under the disease progress curve (AUDPC) was calculated for each genotype. Anthracnose severity was also evaluated on the peduncle, rachis and glumes, panicle, and grain. For the characters under study, the site x year and site x year x line interactions accounted for the genotype x environment interactions. Pattern analysis was applied to the environment-standardized matrix of genotype x environment means to analyze these interactions and elucidate genotypic adaptation. None of the lines was completely (hypersensitive) resistant to the disease, but 12 showed high levels of stable resistance to both foliar and panicle anthracnose. Only one was moderately susceptible to both forms of the disease. In addition to identifying varieties that can be grown in zones to which they are adapted, additional genotypes were identified that can serve as sources of resistance in regional breeding programs