Examining Parental Involvement in an Elementary School\u27s Prekindergarten Program

Parental involvement is integral to promoting a high quality school environment. A lack of parent involvement is a problem that currently exists in an urban, public elementary school in Texas. This issue is important because once the parents increase their engagement, the home school relationship can be improved. The purpose of this qualitative case study was to investigate how pre-kindergarten parents at the study elementary school perceive parent involvement and how principals and teachers at the school can help parents improve their involvement. This study is based upon Epstein\u27s theory of 6 types of parent involvement. This study examined parents\u27 perceptions of the role of parental involvement in children\u27s education and the strategies that schools may use to boost parental involvement. Over the course of the 9-month school year, individual interviews were conducted with 14 parents whose children attended the prekindergarten program at this school in Texas. Thematic analysis was used to identify themes. Parents considered their involvement to be important and appreciated receiving school information through phone conferences, newsletters, and an online portal. Parents also reported that health issues, time restraints, and busy work schedules were obstacles to being more involved. Recommendations for improving parental involvement included parent workshops on academic strategies and programming that is scheduled after school to accommodate parents\u27 schedules. This study could contribute to positive social change by encouraging parental involvement at the study site. Additionally, this study could enhance positive social change by encouraging the educational sector to focus on building effective school/family partnerships

Differential expression of the adult specifier E93 in the strepsipteran Xenos vesparum Rossi suggests a role in female neoteny

Holometaboly is a key evolutionary innovation that has facilitated the spectacular radiation of insects. Despite the undeniable advantage of complete metamorphosis, the female of some holometabolous species have lost the typical holometabolous development through neoteny. In Xenos vesparum Rossi (Strepsiptera: Stylopidae), a derived species of the holometabolous endoparasitic order Strepsiptera, neotenic females reach sexual maturity without the pupal and the imaginal stages, thus retaining their larval morphology (with the exception of the anterior part of the body or cephalothorax), while males undergo normal pupal-based metamorphosis. Expression of the “adult-specifier” E93 factor has been shown to be required for proper metamorphosis in holometabolous insects. Here, we investigated the involvement of E93 in female neoteny by cloning XvE93. Interestingly, while we detected a clear up-regulation of XvE93 expression in pupal and adult stages of males, persistent low levels of XvE93 were detected in X. vesparum females. However, a specific up-regulation of XvE93 was observed in the cephalothorax of late 4th female instar larva, which correlates with the occurrence of neotenic-specific features in the anterior part of the female body. Moreover, the same expression dynamic in the cephalothorax and abdomen was also observed for other two critical metamorphic regulators, the anti-metamorphic XvKr-h1 and the pupal specifier XvBr-C. The specific up-regulation of XvE93 and XvBr-C in the female cephalothorax seems to be the result of an increase in 20-hydroxyecdysone (20E) signaling in this region for we detected higher expression levels of the 20E-dependent nuclear receptors XvHR3 and XvE75 in the cephalothorax. Overall, our results detect a sex-specific expression pattern of critical metamorphic genes in X. vesparum, suggesting that neoteny in Strepsiptera results from the modification of the normal expression of E93, Br-C and Kr-h1 genes

Gene expression dynamics of natural assemblages of heterotrophic flagellates during bacterivory

17 pages, 5 figures, 2 tables, supplementary information https://doi.org/10.1186/s40168-023-01571-5.-- Availability of data and materials: Raw data for “Jul17” incubation were already published at the NCBI BioSample database with accession number SAMN11783926 [38]. For the rest of the incubations, raw data are deposited at NCBI with accession number PRJNA973582. Assemblies, quantification, and functional annotation tables are available at FigShare (https://doi.org/10.6084/m9.figshare.22801697), and all code used for data processing and analyses is available at GitHub (https://github.com/aleixop/metaT_bacterivory)Background: Marine heterotrophic flagellates (HF) are dominant bacterivores in the ocean, where they represent the trophic link between bacteria and higher trophic levels and participate in the recycling of inorganic nutrients for regenerated primary production. Studying their activity and function in the ecosystem is challenging since most of the HFs in the ocean are still uncultured. In the present work, we investigated gene expression of natural HF communities during bacterivory in four unamended seawater incubations. Results: The most abundant species growing in our incubations belonged to the taxonomic groups MAST-4, MAST-7, Chrysophyceae, and Telonemia. Gene expression dynamics were similar between incubations and could be divided into three states based on microbial counts, each state displaying distinct expression patterns. The analysis of samples where HF growth was highest revealed some highly expressed genes that could be related to bacterivory. Using available genomic and transcriptomic references, we identified 25 species growing in our incubations and used those to compare the expression levels of these specific genes. Conclusions: Our results indicate that several peptidases, together with some glycoside hydrolases and glycosyltransferases, are more expressed in phagotrophic than in phototrophic species, and thus could be used to infer the process of bacterivory in natural assemblagesOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was supported by the Spanish Ministry of Science and Innovation projects ALLFLAGS (CTM2016-75083-R), DIVAS (PID2019-108457RB-I00) and the “Severo Ochoa Centre of Excellence” accreditation (CEX2019-000928-S), and the European Union projects SINGEK (H2020-MSCA-ITN-2015–675752) and GENEXLAB (EUR2022-134047, MICINN). AO was supported by a Spanish FPI grant. E.D.S. was supported by the MSCA-IF SMART (CZ.02.2.69/0.0/0.0/20_079/0017809)Peer reviewe