Complete gradient-LC-ESI system on a chip for protein analysis

This paper presents the first fully integrated gradient-elution liquid chromatography-electrospray ionization (LC-ESI) system on a chip. This chip integrates a pair of high-pressure gradient pumps, a sample injection pump, a passive mixer, a packed separation column, and an ESI nozzle. We also present the successful on-chip separation of protein digests by reverse phase (RP)-LC coupled with on-line mass spectrometer (MS) analysis

Phase transition in a spring-block model of surface fracture

A simple and robust spring-block model obeying threshold dynamics is introduced to study surface fracture of an overlayer subject to stress induced by adhesion to a substrate. We find a novel phase transition in the crack morphology and fragment-size statistics when the strain and the substrate coupling are varied. Across the transition, the cracks display in succession short-range, power-law and long-range correlations. The study of stress release prior to cracking yields useful information on the cracking process.Comment: RevTeX, 4 pages, 4 Postscript figures included using epsfi

Electromagnetic modes of Maxwell fisheye lens

We provide an analysis of the radial structure of TE and TM modes of the Maxwell fisheye lens, by means of Maxwell equations as applied to the fisheye case. Choosing a lens of size R = 1 cm, we plot some of the modes in the infrared range.Comment: 2+6 pages in Latex, 3 figures to be found in the published referenc

Diffraction of light by a planar aperture in a metallic screen

We present a complete derivation of the formula of Smythe [Phys.Rev.72, 1066 (1947)] giving the electromagnetic field diffracted by an aperture created in a perfectly conducting plane surface. The reasoning, valid for any excitating field and any hole shape, makes use only of the free scalar Green function for the Helmoltz equation without any reference to a Green dyadic formalism. We compare our proof with the one previously given by Jackson and connect our reasoning to the general Huygens Fresnel theorem.Comment: J. Math. Phys. 47, 072901 (2006

Salicylaldehyde hydrazones: buttressing of outer sphere hydrogen-bonding and copper-extraction properties

Salicylaldehyde hydrazones are weaker copper extractants than their oxime derivatives, which are used in hydrometallurgical processes to recover ~20 % of the world’s copper. Their strength, based on the extraction equilibrium constant Ke, can be increased by nearly three orders of magnitude by incorporating electron-withdrawing or hydrogen-bond acceptor groups (X) ortho to the phenolic OH group of the salicylaldehyde unit. Density functional theory calculations suggest that the effects of the 3-X substituents arise from a combination of their influence on the acidity of the phenol in the pH-dependent equilibrium, Cu2+ + 2Lorg ⇌ [Cu(L–H)2]org + 2H+, and on their ability to ‘buttress’ interligand hydrogen bonding by interacting with the hydrazone N–H donor group. X-ray crystal structure determination and computed structures indicate that in both the solid state and the gas phase, coordinated hydrazone groups are less planar than coordinated oximes and this has an adverse effect on intramolecular hydrogen-bond formation to the neighbouring phenolate oxygen atoms

A two-state model for helicase translocation and unwinding of nucleic acids

Helicases are molecular motors that unwind double-stranded nucleic acids (dsNA), such as DNA and RNA). Typically a helicase translocates along one of the NA single strands while unwinding and uses adenosine triphosphate (ATP) hydrolysis as an energy source. Here we model of a helicase motor that can switch between two states, which could represent two different points in the ATP hydrolysis cycle. Our model is an extension of the earlier Betterton-J\"ulicher model of helicases to incorporate switching between two states. The main predictions of the model are the speed of unwinding of the dsNA and fluctuations around the average unwinding velocity. Motivated by a recent claim that the NS3 helicase of Hepatitis C virus follows a flashing ratchet mechanism, we have compared the experimental results for the NS3 helicase with a special limit of our model which corresponds to the flashing ratchet scenario. Our model accounts for one key feature of the experimental data on NS3 helicase. However, contradictory observations in experiments carried out under different conditions limit the ability to compare the model to experiments.Comment: minor modification

Re-orientation Transition in Molecular Thin Films: Potts Model with Dipolar Interaction

We study the low-temperature behavior and the phase transition of a thin film by Monte Carlo simulation. The thin film has a simple cubic lattice structure where each site is occupied by a Potts parameter which indicates the molecular orientation of the site. We take only three molecular orientations in this paper which correspond to the 3-state Potts model. The Hamiltonian of the system includes: (i) the exchange interaction $J_{ij}$ between nearest-neighbor sites $i$ and $j$ (ii) the long-range dipolar interaction of amplitude $D$ truncated at a cutoff distance $r_c$ (iii) a single-ion perpendicular anisotropy of amplitude $A$. We allow $J_{ij} =J_s$ between surface spins, and $J_{ij}=J$ otherwise. We show that the ground state depends on the the ratio $D/A$ and $r_c$. For a single layer, for a given $A$, there is a critical value $D_c$ below (above) which the ground-state (GS) configuration of molecular axes is perpendicular (parallel) to the film surface. When the temperature $T$ is increased, a re-orientation transition occurs near $D_c$: the low-$T$ in-plane ordering undergoes a transition to the perpendicular ordering at a finite $T$, below the transition to the paramagnetic phase. The same phenomenon is observed in the case of a film with a thickness. We show that the surface phase transition can occur below or above the bulk transition depending on the ratio $J_s/J$. Surface and bulk order parameters as well as other physical quantities are shown and discussed.Comment: 7 pages, 11 figures, submitted for publicatio

Integrated Flexible Ocular Coil for Power and Data Transfer in Retinal Prostheses

A microfabricated and fully-implantable coil for use as a power and data transfer component for retinal prostheses is presented. Compared with traditional hand-made ocular coils, this parylene-based device is thin and flexible with 10 turns of thin-film metal wires and a thickness of less than 10 µm. In addition, the entire coil structure can be heat-formed on a mold to match the eye's curvature for extraocular implantation. Because it is made using parylene thin-film technology, this coil can be directly integrated with multielectrode arrays and with parylene-based packages incorporating application specific integrated circuits (ASICs) or discrete electrical components such as chip capacitors. This coil thus enables the fabrication and implantation of a fully microfabricated system for retinal prostheses

Enhanced dispersion interaction in confined geometry

The dispersion interaction between two point-like particles confined in a dielectric slab between two plates of another dielectric medium is studied within a continuum (Lifshitz) theory. The retarded (Casimir-Polder) interaction at large inter-particle distances is found to be strongly enhanced as the mismatch between the dielectric permittivities of the two media is increased. The large-distance interaction is multiplied due to confinement by a factor of $(33\gamma^{5/2}+13\gamma^{-3/2})/46$ at zero temperature, and by $(5\gamma^2+\gamma^{-2})/6$ at finite temperature, \gamma=\ein(0)/\eout(0) being the ratio between the static dielectric permittivities of the inner and outer media. This confinement-induced amplification of the dispersion interaction can reach several orders of magnitude.Comment: 4 page