Amine functionalization of cholecyst-derived extracellular matrix with generation 1 PAMAM dendrimer

This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Biomacromolecules, copyright © American Chemical Society after peer review. To access the final edited and published work, see http://pubs.acs.org/doi/pdf/10.1021/bm701055k.A method to functionalize cholecyst-derived extracellular matrix (CEM) with free amine groups was established in an attempt to improve its potential for tethering of bioactive molecules. CEM was incorporated with Generation-1 polyamidoamine (G1 PAMAM) dendrimer by using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide and N-hydroxysuccinimide cross-linking system. The nature of incorporation of PAMAM dendrimer was evaluated using shrink temperature measurements, Fourier transform infrared (FTIR) assessment, ninhydrin assay, and swellability. The effects of PAMAM incorporation on mechanical and degradation properties of CEM were evaluated using a uniaxial mechanical test and collagenase degradation assay, respectively. Ninhydrin assay and FTIR assessment confirmed the presence of increasing free amine groups with increasing quantity of PAMAM in dendrimer-incorporated CEM (DENCEM) scaffolds. The amount of dendrimer used was found to be critical in controlling scaffold degradation, shrink temperature, and free amine content. Cell culture studies showed that fibroblasts seeded on DENCEM maintained their metabolic activity and ability to proliferate in vitro. In addition, fluorescence cell staining and scanning electron microscopy analysis of cell-seeded DENCEM showed preservation of normal fibroblast morphology and phenotype

Non-equilibrium emission of complex fragments from p+Au collisions at 2.5 GeV proton beam energy

Energy and angular dependence of double differential cross sections d$^2\sigma$/d$\Omega$dE was measured for reactions induced by 2.5 GeV protons on Au target with isotopic identification of light products (H, He, Li, Be, and B) and with elemental identification of heavier intermediate mass fragments (C, N, O, F, Ne, Na, Mg, and Al). It was found that two different reaction mechanisms give comparable contributions to the cross sections. The intranuclear cascade of nucleon-nucleon collisions followed by evaporation from an equilibrated residuum describes low energy part of the energy distributions whereas another reaction mechanism is responsible for high energy part of the spectra of composite particles. Phenomenological model description of the differential cross sections by isotropic emission from two moving sources led to a very good description of all measured data. Values of the extracted parameters of the emitting sources are compatible with the hypothesis claiming that the high energy particles emerge from pre-equilibrium processes consisting in a breakup of the target into three groups of nucleons; small, fast and hot fireball of $\sim$ 8 nucleons, and two larger, excited prefragments, which emits the light charged particles and intermediate mass fragments. The smaller of them contains $\sim$ 20 nucleons and moves with velocity larger than the CM velocity of the proton projectile and the target. The heavier prefragment behaves similarly as the heavy residuum of the intranuclear cascade of nucleon-nucleon collisions. %The mass and charge dependence of the total production cross %sections was extracted from the above analysis for all observed %reaction products. This dependence follows the power low behavior %(A$^{-\tau}$ or Z$^{-\tau}$)

Advances in Corrosion Casting Methods

This paper briefly discusses the concept of corrosion cast preparation (primarily of blood vessels), the use of the scanning electron microscope (SEM) to study these casts and the observations which can be made, together with the merits and the limitations in various applications. A number of reviews and surveys are quoted in which the different injection media, injection methods, animal preparations and corrosion procedures are described. A new procedure of cleaning the corrosion casts with sodium hydroxide and Triton X-100 is described. The observations which can be made are listed and illustrated both on the cellular level as well as in organ systems as a whole. The discussion centers around some common misconceptions, the feasibility in various applications and the limitations of the method. The conclusion is that the method has proven to be useful especially in conjunction with other methods. Moreover, while the concept of the method may be very straight-forward the approach and the interpretation often need careful consideration and might not be as straight-forward as one tends to expect from the simple sounding principle

Comparison of nonequilibrium processes in p+Ni and p+Au collisions at GeV energies

The energy and angular dependence of double differential cross sections d2sigma/dOmega dE were measured for p, d, t, 3,4,6He, 6,7,8Li, 7,9,10Be, 10,11B, and C produced in collisions of 1.2, 1.9, and 2.5 GeV protons with a Ni target. The shape of the spectra and angular distributions does almost not change whereas the absolute value of the cross sections increases by a factor about 1.7 for all ejectiles in this beam energy range. It was found that energy and angular dependencies of the cross sections cannot be reproduced by the microscopic model of intranuclear cascade with coalescence of nucleons and the statistical model for evaporation of particles from excited, equilibrated residual nuclei. The inclusion of nonequilibrium processes, described by a phenomenological model of the emission from fast and hot moving sources, resulting from break-up of the target nucleus by impinging proton, leads to very good reproduction of data. Cross sections of these processes are quite large, exhausting approximately half of the total production cross sections. Due to good reproduction of energy and angular dependencies of d2sigma/dOmega dE by model calculation it was possible to determine total production cross sections for all studied ejectiles. Results obtained in this work point to the analogous reaction mechanism for proton induced reactions on Ni target as that observed previously for Au target in the same beam energy range.Comment: 11 pages, 10 figures

Variation of nonequilibrium processes in p+Ni system with beam energy

The energy and angular dependence of double differential cross sections dsigma/dOmega dE were measured for p, d, t, 3,4He, 6,7Li, 7,9Be, and 10,11B produced in collisions of 0.175 GeV protons with Ni target. The analysis of measured dfferential cross sections allowed to extract total production cross sections for ejectiles listed above. The shape of the spectra and angular distributions indicate the presence of other nonequilibrium processes besides the emission of nucleons from the intranuclear cascade, and besides the evaporation of various particles from remnants of intranuclear cascade. These nonequilibrium processes consist of coalescence of nucleons into light charged particles during the intranuclear cascade, of the fireball emission which contributes to the cross sections of protons and deuterons, and of the break-up of the target nucleus which leads to the emission of intermediate mass fragments. All such processes were found earlier at beam energies 1.2, 1.9, and 2.5 GeV for Ni as well as for Au targets, however, significant differences in properties of these processes at high and low beam energy are observed in the present study.Comment: 10 pages, 9 figure

Competition of coalescence and "fireball" processes in nonequilibrium emission of light charged particles from p+Au collisions

The energy and angular dependence of double differential cross sections was measured for p,d,t,He,Li,Be, and B isotopes produced in collisions of 1.2 and 1.9 GeV protons with Au target. The shape of the spectra and angular distributions almost does not change in the beam energy range from 1.2 to 2.5 GeV, however, the absolute value of the cross sections increases for all ejectiles. A phenomenological model of two emitting, moving sources reproduces very well spectra and angular distributions of intermediate mass fragments. Double differential cross sections for light charged particles (LCP) were analyzed in the frame of the microscopic model of intranuclear cascade (INC) with coalescence of nucleons and statistical model for evaporation of particles from excited residual nuclei. Energy and angular dependencies of data agree satisfactorily neither with predictions of microscopic intranuclear cascade calculations for protons, nor with coalescence calculations for other LCP. Phenomenological inclusion of another reaction mechanism - emission of LCP from a "fireball", i.e., fast and hot moving source - combined with the microscopic model calculations of INC, coalescence and evaporation of particles leads to very good description of the data. It was found that nonequilibrium processes are very important for production of LCP. They exhaust 40-80% of the total cross sections - depending on the emitted particles. Coalescence and "fireball" emission give comparable contributions to the cross sections with exception of 3He data where coalescence clearly dominates. The ratio of sum of all nonequilibrium processes to those proceeding through stage of statistical equilibrium does almost not change in the beam energy range from 1.2 GeV to 2.5 GeV for all light charged particles.Comment: 14 pages, 12 figures, IV tables, \pacs{25.40.-h,25.40.Sc,25.40.Ve