MathWeb: A Concurrent Image Analysis Tool Suite for Multi-spectral Data Fusion

This paper describes a preliminary approach to the fusion of multi-spectral image data for the analysis of cervical cancer. The long-term goal of this research is to define spectral signatures and automatically detect cancer cell structures. The approach combines a multi-spectral microscope with an image analysis tool suite, MathWeb. The tool suite incorporates a concurrent Principal Component Transform (PCT) that is used to fuse the multi-spectral data. This paper describes the general approach and the concurrent PCT algorithm. The algorithm is evaluated from both the perspective of image quality and performance scalability

A study of school plant needs and a new high school for Revere, Massachusetts

Thesis (M.Arch.) Massachusetts Institute of Technology. Dept. of Architecture, 1954.Accompanying drawings held by MIT Museum.Bibliography: leaves [134]-136.by Thorkel Myron W. Haaland.M.Arch

Thermal receptivity of free convective flow from a heated vertical surface: linear waves

Numerical techniques are used to study the receptivity to small-amplitude thermal disturbances of the boundary layer flow of air which is induced by a heated vertical flat plate. The fully elliptic nonlinear, time-dependent Navier–Stokes and energy equations are first solved to determine the steady state boundary-layer flow, while a linearised version of the same code is used to determine the stability characteristics. In particular we investigate (i) the ultimate fate of a localised thermal disturbance placed in the region near the leading edge and (ii) the effect of small-scale surface temperature oscillations as means of understanding the stability characteristics of the boundary layer. We show that there is a favoured frequency of excitation for the time-periodic disturbance which maximises the local response in terms of the local rate of heat transfer. However the magnitude of the favoured frequency depends on precisely how far from the leading edge the local response is measured. We also find that the instability is advective in nature and that the response of the boundary layer consists of a starting transient which eventually leaves the computational domain, leaving behind the large-time time-periodic asymptotic state. Our detailed numerical results are compared with those obtained using parallel flow theory

Hyperspectral microarray scanning: impact on the accuracy and reliability of gene expression data

BACKGROUND: Commercial microarray scanners and software cannot distinguish between spectrally overlapping emission sources, and hence cannot accurately identify or correct for emissions not originating from the labeled cDNA. We employed our hyperspectral microarray scanner coupled with multivariate data analysis algorithms that independently identify and quantitate emissions from all sources to investigate three artifacts that reduce the accuracy and reliability of microarray data: skew toward the green channel, dye separation, and variable background emissions. RESULTS: Here we demonstrate that several common microarray artifacts resulted from the presence of emission sources other than the labeled cDNA that can dramatically alter the accuracy and reliability of the array data. The microarrays utilized in this study were representative of a wide cross-section of the microarrays currently employed in genomic research. These findings reinforce the need for careful attention to detail to recognize and subsequently eliminate or quantify the presence of extraneous emissions in microarray images. CONCLUSION: Hyperspectral scanning together with multivariate analysis offers a unique and detailed understanding of the sources of microarray emissions after hybridization. This opportunity to simultaneously identify and quantitate contaminant and background emissions in microarrays markedly improves the reliability and accuracy of the data and permits a level of quality control of microarray emissions previously unachievable. Using these tools, we can not only quantify the extent and contribution of extraneous emission sources to the signal, but also determine the consequences of failing to account for them and gain the insight necessary to adjust preparation protocols to prevent such problems from occurring

Statistical Analysis of Microarray Data with Replicated Spots: A Case Study with Synechococcus WH8102

Until recently microarray experiments often involved relatively few arrays with only a single representation of each gene on each array. A complete genome microarray with multiple spots per gene (spread out spatially across the array) was developed in order to compare the gene expression of a marine cyanobacterium and a knockout mutant strain in a defined artificial seawater medium. Statistical methods were developed for analysis in the special situation of this case study where there is gene replication within an array and where relatively few arrays are used, which can be the case with current array technology. Due in part to the replication within an array, it was possible to detect very small changes in the levels of expression between the wild type and mutant strains. One interesting biological outcome of this experiment is the indication of the extent to which the phosphorus regulatory system of this cyanobacterium affects the expression of multiple genes beyond those strictly involved in phosphorus acquisition

Laser acceleration of ion beams

We consider methods of charged particle acceleration by means of high-intensity lasers. As an application we discuss a laser booster for heavy ion beams provided, e.g. by the Dubna nuclotron. Simple estimates show that a cascade of crossed laser beams would be necessary to provide additional acceleration to gold ions of the order of GeV/nucleon.Comment: 4 pages, 4 figures, Talk at the Helmholtz International Summer School "Dense Matter in heavy Ion Collisions and Astrophysics", August 21 - September 1, 2006, JINR Dubna, Russia; v2, misprints correcte

Can magnetotail reconnection produce the auroral intensities observed in the conjugate ionosphere?

[1] In a recent case study, Borg et al. (2007) reported that an inverted V structure, caused by a field-aligned potential drop of 30 kV producing very strong X-ray aurora, was found in connection with tail reconnection. However, the in situ particle measurements indicated clearly that the particles responsible for the X-ray aurora were not accelerated by the reconnection process. In this article, we report the predicted auroral intensities of thirteen reconnection events where Cluster passed through the reconnection region. For six of the events, global auroral imaging data were available and the predicted auroral intensities could be compared with the observed intensities. Our main findings are as follows: (1) Acceleration in the reconnection region is generally not sufficient to account for the observed auroral intensities. (2) Additional acceleration between the reconnection region and the ionosphere is needed to explain the auroral intensities. Although we see signatures that point toward potential drops at the flanks of bursty bulk flows (BBFs), we also find signatures of Alfvén wave accelerated electrons at 700 km and we are not able to determine the most likely acceleration mechanism. (3) The reconnection events are observed 2–14 min after substorm onset and indicate that reconnection is an expanding process observed along the poleward boundary of the aurora.publishedVersio

Strengthening patient-centred communication in rural Ugandan health centres: A theory-driven evaluation within a cluster randomized trial.

This article describes a theory-driven evaluation of one component of an intervention to improve the quality of health care at Ugandan public health centres. Patient-centred services have been advocated widely, but such approaches have received little attention in Africa. A cluster randomized trial is evaluating population-level outcomes of an intervention with multiple components, including 'patient-centred services.' A process evaluation was designed within this trial to articulate and evaluate the implementation and programme theories of the intervention. This article evaluates one hypothesized mechanism of change within the programme theory: the impact of the Patient Centred Services component on health-worker communication. The theory-driven approach extended to evaluation of the outcome measures. The study found that the proximal outcome of patient-centred communication was rated 10 percent higher (p < 0.008) by care seekers consulting with the health workers who were at the intervention health centres compared with those at control health centres. This finding will strengthen interpretation of more distal trial outcomes