Frequency multiplication in high-energy electron beams Semiannual progress report, 1 Apr. - 1 Oct. 1967

High energy electron beam studies dealing with nonlinear analysis of beam-plasma interactions, cyclotron harmonic instabilities, and frequency multiplicatio

Microwave device investigations Semiannual progress report, 1 Apr. - 1 Oct. 1968

Beam-plasma interactions, cyclotron harmonic instabilities, harmonic generation in beam-plasma system, relativistic electron beam studies, and materials test

Microwave device investigations Semiannual progress report, 1 Oct. 1969 - 1 Apr. 1970

Beam-plasma interactions, cyclotron harmonic instability study, and millimeter and submillimeter wave detection by paramagnetic material

Frequency multiplication in high-energy electron beams Semiannual progress report, Oct. 1, 1966 - Apr. 1, 1967

Frequency multiplication in high energy electron beam

Microwave device investigations Semiannual progress report, 1 Apr. - 1 Oct. 1969

Summaries of progress in beam plasma interactions, paramagnetic and bulk semiconductor materials, and avalanche diode

Microwave device investigations Semiannual progress report, 1 Oct. 1968 - 1 Apr. 1969

Beam plasma amplification, harmonic generation, and coupling scheme

Frequency multiplication in high-energy electron beams Semiannual progress report, 1 Oct. 1967 - 31 Mar. 1968

Electron beam-plasma interactions, cyclotron harmonic instabilities, paramagnetic and semiconductor materials, and harmonic current generatio

Heterologous prime-boost-boost immunisation of Chinese cynomolgus macaques using DNA and recombinant poxvirus vectors expressing HIV-1 virus-like particles

Background: There is renewed interest in the development of poxvirus vector-based HIV vaccines due to the protective effect observed with repeated recombinant canarypox priming with gp120 boosting in the recent Thai placebo-controlled trial. This study sought to investigate whether a heterologous prime-boost-boost vaccine regimen in Chinese cynomolgus macaques with a DNA vaccine and recombinant poxviral vectors expressing HIV virus-like particles bearing envelopes derived from the most prevalent clades circulating in sub-Saharan Africa, focused the antibody response to shared neutralising epitopes. Methods: Three Chinese cynomolgus macaques were immunised via intramuscular injections using a regimen composed of a prime with two DNA vaccines expressing clade A Env/clade B Gag followed by boosting with recombinant fowlpox virus expressing HIV-1 clade D Gag, Env and cholera toxin B subunit followed by the final boost with recombinant modified vaccinia virus Ankara expressing HIV-1 clade C Env, Gag and human complement protein C3d. We measured the macaque serum antibody responses by ELISA, enumerated T cell responses by IFN-gamma ELISpot and assessed seroneutralisation of HIV-1 using the TZM-bl beta-galactosidase assay with primary isolates of HIV-1. Results: This study shows that large and complex synthetic DNA sequences can be successfully cloned in a single step into two poxvirus vectors: MVA and FPV and the recombinant poxviruses could be grown to high titres. The vaccine candidates showed appropriate expression of recombinant proteins with the formation of authentic HIV virus-like particles seen on transmission electron microscopy. In addition the b12 epitope was shown to be held in common by the vaccine candidates using confocal immunofluorescent microscopy. The vaccine candidates were safely administered to Chinese cynomolgus macaques which elicited modest T cell responses at the end of the study but only one out of the three macaques elicited an HIV-specific antibody response. However, the antibodies did not neutralise primary isolates of HIV-1 or the V3-sensitive isolate SF162 using the TZM-bl b-galactosidase assay. Conclusions: MVA and FP9 are ideal replication-deficient viral vectors for HIV-1 vaccines due to their excellent safety profile for use in humans. This study shows this novel prime-boost-boost regimen was poorly immunogenic in Chinese cynomolgus macaques

A Miniature Laser Desorption/Ionization Time-of-Flight Mass Spectrometer for in Situ Analysis of Mars Surface Composition and Identification of Hazard in Advance of Future Manned Exploration

Future landed missions to Mars will be guided by two strategic directions: (1) sample return to Earth, for comprehensive compositional analyses, as recommended by the 2011 NRC Planetary Decadal Survey; and (2) preparation for human exploration in the 2030s and beyond, as laid out by US space policy. The resultant mission architecture will likely require high-fidelity in situ chemical/organic sample analyses within an extremely constrained resource envelope. Both science goals (e.g., MEPAG Goal 1, return sample selection, etc.) as well as identification of any potential toxic and biological hazards to humans, must be addressed. Over the past several years of instrument development, we have found that the adaptable, compact, and highly capable technique of laser desorption/ionization time-of-flight mass spectrometry (LD-TOF-MS) has significant potential to contribute substantially to these dual objectives. This concept thus addresses Challenge Area 1: instrumentation and Investigation Approaches

Plasma Electronics

Contains reports on five research projects.National Science Foundation (Grant GK-19)Lincoln Laboratory (Purchase Order DDL BB-107)U. S. Air Force (Contract AF 19(628)-500)U. S. Atomic Energy Commission (Contract AT(30-1)-3221)U. S. Atomic Energy Commission (Contract AT(30-1)-3285