Isolation and identification of fungal communities from vegetable wastes composts,

Compost preparation was carried out at Department of Botany, Bangalore University Bangalore during 2013 and 2014 by using Cabbage, tomato fresh cow dung and mixed vegetable waste. The experiments were laid down in a completely replicated randomized block design with three replication for each treatment. The qualitative and quantitative composition of individual vegetable composts (Tomato waste, Cabbage waste and mixed vegetable waste) were used for the isolation. The fungi were isolated on Potato Dextrose Agar (PDA) and at different temperature 50-55°C. Substantially qualitative differences in the species composition of different vegetables composts were observed. A total of 11 entities were isolated 3 from tomato waste compost, 2 from cabbage waste compost and 4 from mixed vegetable waste at 50°C and 3 at 55°C respectively. This report demonstrates that the qualitative and quantitative characterization of individual composts and fungal communities essential for best industrial application

MOLECULAR DOCKING STUDIES ON THIADIAZOLE DERIVATIVES AS PROTEIN KINASE INHIBITORS

Objective: In the present study, a novel series of 1, 3, 4-thiadiazole derivatives were docked against the mycobacterium tuberculosis protein kinase G. 1, 3, 4–thiadiazole derivatives with a modified primary amine group at 5th position were used for docking studies. Methods: The three-dimensional structure of the protein was obtained from PDB, and its active sites were predicted. The structures of all the compounds were drawn using chemdraw software version 8.0. The docking studies were done by using schrödinger software against the enzyme protein kinase G. Totally eighteen compounds was synthesized based on glide score Results: In this Docking study the thiadiazole analogues were showing good binding energy. The amino acids residues GLU588, SER412, GLY410 and GLU 628 in the kinase domain active site form hydrogen bonds with the ligand. Conclusion: The compounds D34, D16, D7, D25, D15, and D27 showed better interaction with protein kinase G (pknG) more than the other drug molecule

Study of the obstetrics and perinatal outcome of in vitro fertilization pregnancies at tertiary care centre

Background: Assisted reproductive techniques (ART) has been a boon to the unfortunate. However, the outcomes aren’t the same to all. The study has observed the outcomes in IVF conceived patients. The pregnant women were followed-up throughout their antenatal and post-natal (7 days) period and an appropriate meta-analysis was made.Methods: This is an Observational Prospective study. It was conducted at an IVF center of tertiary hospital. With valid written informed consents 96 patients were recruited and observed for18 months. Data collected by Universal sampling method, analyzed and interpreted.Results: A total 65.6% belonged above 30 years, 67.7% were primigravida and 7.3% had history of previous ectopic pregnancy. In 23.75% multifetal pregnancy was significant and 20.63% had OS tightening done. Most common reason for opting IVF was multiple failed IUI (71.6%). More than 40% were delivered by caesarean section. 91.1% of babies cried immediately after birth and 50.4% babies required NICU admission.13.4% babies had neonatal death and 3.1% were IUFD. 58.30% pregnancies were singleton. Mean birth weight was 1.87 Kg and 72.6% children were born low birth weight. Statistical significance (p<0.001) was found in between no. of foetuses delivered and preterm delivery. 56.3% birth were preterm birth. PIH and GDM complication is most common in multiple gestations observed in 10.7% of pregnancies.Conclusions: Assisted Reproductive technologies has helped the infertile couples. Outcomes could be unfruitful in few, while others may have a complicated antenatal period. Preterm deliveries are more common in multiple gestation thus complementing the lower birth weights. Comorbidities significantly affected obstetric outcome

Synthesis and Characterization of Silica from Ragi Husk Ash (Finger Millet) by a Sol-gel Method

Silica aerogel, a mesoporous material, was prepared from ragi husk ash by sol–gel method and dried under atmospheric pressure. In this method, ragi  husk ash, which is rich in silica, was extracted with sodium hydroxide solution to produce a sodium silicate solution. This solution was neutralized with acid to form a silica aerogel. The aged gel was washed carefully by de ionized water and ethanol and finally dried under atmospheric air. A temperature of 7000C was found to be optimum ashing temperature with maximum amorphous silica content. From a morphological analysis obtained by SEM and diffraction patterns (XRD), a longitudinal fibrous and amorphous structure was observed for Ragi Husk. FTIR characterization indicated the presence of silanol and siloxane groups. Thermo gravimetric was used to characterize burning behaviour and also to determine the activation energies. This economic technology, as applied to waste material, also provides many benefits to the local agro-industry. Keywords: Ragi husk Ash, Sol- gel method, Amorphous silica, Aeroge

Growth of Nursery Grown Micro Propagated Bamboo (Bambusa Tulda .L) Inoculated with Arbuscular Mycorrhizal Fungus and Plant Growth Promoting Rhizobacteria (PGPR)

A nursery experiment was conducted to investigate the effect of bio inoculants AMF (Glomus mosseae) Frateuria aurantia (potassium mobilizer), Azospirillium (Free nitrogen fixer) on growth promotion of micro propagated thorn less bamboo (Bambusa tulda .L).Bamboo seedlings were inoculated singly or in triple inoculation with Glomus mosseae, Frateuria aurantia and Azospirillium brasilense. The mycorrhizal spore load and per cent root colonization were maximum with triple inoculated plants and least in control and uninoculated plants. - See more at: http://www.worldwidejournals.com/international-journal-of-scientific-research-(IJSR)/articles.php?val=MzMzNw==&b1=89&k=23#sthash.tKy9r1Nv.dpu

Preparation and Characterization of Silica Material from Rice Husk Ash – An Economically Viable Method

Rice husk is a form of agricultural biomass that provides an abundant silicon source. Rice husks are widely burnt in agricultural fields in India because it is difficult to find other uses for them. Farmers burn rice hulls usually under incomplete combustion conditions to avoid accidental fires. The objective of this study was to develop a new method of amorphous silica was prepared from rice husk ash by sol - gel method. Initially received from Rice husk ash was calcined at 4000C, 5000C, 6000C and 7000C for 5 hrs to remove the volatiles in the sample and determine the amorphous structure of SiO2. Next, the thermally treated RHA was mixed with alkali solution to produce sodium silicate solution and precipitated silica was produced by the neutralization of sodium silicate solution. Rice Husks soaked in nitric acid produced the maximum amount of the sodium silicate solution and precipitated silica. Sodium oxide (Na2O) content and silica (SiO2) content in the sodium silicate solution were also determined. Extracted precipitated silica particles were characterized by Fourier transform infrared (FTIR), X-Ray diffraction and Optical microscopy techniques. The chemical composition of silica was confirmed by FTIR and SEM with EDX.. Highly pure amorphous silica was derived from rice husk ash was confirmed by XRD pattern. The morphology of the obtained materials was analyzed by SEM. At optimized conditions, a nano sized highly pure silica was produced with a high reactivity and 99.9% amorphous in form. This economic technology as applied to waste material also provides many benefits to the local agro industry. Thus this paper may be providing a low cost and simple method to prepare functional materials. Keywords: Rice husk ash, Silica gel, Minerals, Amorphous material, Agricultural bio-wast

Reproductive potential in a case of Mosaic Turners syndrome with 46XY karyotype

This article describes a case of 18year-old-female who presented with primary amenorrhea, phenotypic features of Turners syndrome, which was confirmed later by Karyotype to have mosaic 45XO(8)/46XY(22). She had delayed puberty and proved (hormonally) to have ovarian failure, with absent Mullerian structures (radiologically and laparoscopy)