Circadian variation in gastric vagal afferent mechanosensitivity

Food intake is coordinated to cellular metabolism by clock gene expression with a master clock in the suprachiasmatic nucleus synchronized by light exposure. Gastric vagal afferents play a role in regulating food intake, but it is unknown whether they exhibit circadian variation in their mechanosensitivity. We aimed to determine whether gastric vagal afferents express clock genes and whether their response to mechanical stimuli oscillates throughout the light/dark cycle. Nodose ganglia were collected from 8-week-old female C57BL/6 mice every 3 h starting at lights off (1800 h) to quantify Bmal1, Per1, Per2, and Nr1d1 mRNA by qRT-PCR. Additionally in vitro single-fiber recordings of gastric vagal mechanoreceptors were taken at all time points. Per1, Per2, Bmal1, and Nr1d1 mRNA is expressed in the nodose ganglia and levels oscillated over a 24 h period. In mice fed ad libitum, gastric content was 3 times higher at 0000 h and 0300 h than 1200 h. The response of tension receptors to 3 g stretch was reduced by up to 70% at 2100 h, 0000 h, and 0300 h compared with 1200 h. Gastric mucosal receptor response to stroking with a 50 mg von Frey hair was 3 times greater at 1200 h and 1500 h than the response at 0000 h. Similar findings were obtained in mice fasted for 6 h or maintained in darkness for 3 d before study. Therefore, these changes do not result from food intake or the light/dark cycle. Thus, gastric vagal mechanoreceptors display circadian rhythm, which may act to control food intake differentially at different times of the day.Stephen J. Kentish, Claudine L. Frisby, David J. Kennaway, Gary A. Wittert, and Amanda J. Pag

Selected Campaign Tactics Permitted Under the National Labor Relations Act

The thrust of this discussion is to concentrate on several tactics utilized mainly by employers (Soliciting and/or Remedying Grievances during an Election Campaign and Interrogation and Polling) and a tactic used solely by the union (Waiver of Initiation Fees). Following these discussions, a chapter will be devoted to Interference with the Board\u27s Election Process by both parties. Finally, the issue of Misrepresentations in an election campaign will be discussed in depth as this issue is very important today in light of the ever changing approach of the Board over the past several decades

Quasi In Rem Jurisdication; Minimum Contacts; State Statutes; Intermeat, Inc. v. American Poultry, Inc.

The decision of Intermeat, Inc. v. American Poultry, Inc. is the first decision rendered by a federal appeals court based on the United States Supreme Court decision of Shaffer v. Heitner. The Shaffer Court handed down a landmark decision in 1977 that appeared at first light to aim the principles of quasi in rem jurisdiction in a new direction. From the date of the decision it appeared that a court could no longer take jurisdiction of a lawsuit based merely on the fact that property of the defendant was located in the state in which the suit was filed. However, in applying the holding of Shaffer to the Intermeat lawsuit, it seems that the decision in Shaffer is not as sweeping as the legal community first thought

Using World-Wide-Web technology for pathology education

In this article, we describe the development of computer-based learning programs for pathology students at Jefferson Medical College. These programs are authored using HTML (HyperText Markup Language), and are available to students on campus and via the internet. Our computer-based learning resources include scheduling information, course goals and objectives, glossary of key words, self-assessment programs and image-based case studies. These educational programs are popular with the students. We recommend the use of World Wide Web technology to improve teaching and learning in pathology education

Oxygen Compatibility of Brass-Filled PTFE Compared to Commonly Used Fluorinated Polymers for Oxygen Systems

Safe and reliable seal materials for high-pressure oxygen systems sometimes appear to be extinct species when sought out by oxygen systems designers. Materials that seal well are easy to find, but these materials are typically incompatible with oxygen, especially in cryogenic liquid form. This incompatibility can result in seals that leak, or much worse, seals that easily ignite and burn during use. Materials that are compatible with oxygen are easy to find, such as the long list of compatible metals, but these metallic materials are limiting as seal materials. A material that seals well and is oxygen compatible has been the big game in the designer's safari. Scientists at the Materials Combustion Research Facility (MCRF), part of NASA/Marshall Space Flight Center (MSFC), are constantly searching for better materials and processes to improve the safety of oxygen systems. One focus of this effort is improving the characteristics of polymers used in the presence of an oxygen enriched environment. Very few systems can be built which contain no polymeric materials; therefore, materials which have good impact resistance, low heat of combustion, high auto-ignition temperature and that maintain good mechanical properties are essential. The scientists and engineers at the Materials Combustion Research Facility, in cooperation with seal suppliers, are currently testing a new formulation of polytetrafluoroethylene (PTFE) with Brass filler. This Brass-filled PTFE is showing great promise as a seal and seat material for high pressure oxygen systems. Early research has demonstrated very encouraging results, which could rank this material as one of the best fluorinated polymers ever tested. This paper will compare the data obtained for Brass-filled PTFE with other fluorinated polymers, such as TFE-Teflon (PTFE) , Kel-F 81, Viton A, Viton A-500, Fluorel , and Algoflon . A similar metal filled fluorinated polymer, Salox-M , was tested in comparison to Brass-filled PTFE to demonstrate the importance of the metal chosen and relative percentage of filler. General conclusions on the oxygen compatibility of this formulation are drawn, with an emphasis on comparing and contrasting the materials performance to the performance of the current state-of-the-art oxygen compatible polymers

A Five-Year Retroactive Analysis of Cut Score Impact: California’s Proposed Supervised Provisional License Program

A five-year cohort of 39,737 examinees who sat for the California Bar Exam (“CBX”) between 2014-18 was analyzed using a simulation model based on actual exam results to evaluate how the minimum passing scores (“cut score”) of 1440, 1390, 1350, 1330, and 1300, if used as qualifying scores for a provisional licensing program, would affect the number of previous examinees, by race and ethnicity, who would qualify to participate within retroactive groupings of five-year, four-year, three-year, two-year, and one-year examinee cohorts.The result of the simulation models indicated that selecting a qualifying score lower than the current California cut score of 1390 will significantly increase both the overall number of eligible participants and the diversity of the group eligible to participate in the proposed alternate licensing program.This study follows an initial study of 85,727 examinees of the CBX from 2009-18 titled, Examining the California Cut Score: An Empirical Analysis of Minimum Competency, Public Protection, Disparate Impact, and National Standards that determined maintaining a high cut score does not result in greater public protection as measured by disciplinary statistics, but does result in excluding minorities from admission to the bar and the practice of law at rates disproportionately higher than Whites

Siting prisons, sighting communities: geographies of objection in a planning process

This paper reviews the planning process for a Scottish prison located near a former mining village. Analysing the letters of objection submitted by residents offers an opportunity to explore local views about prison and community and to relate these to the unique social and spatial history of the area. The planning process itself structured how residents were able to express themselves and defined what counted as a relevant objection. After deconstructing this process, the paper then restores and uses as a framework for analysis three geographies of objection stripped from local responses to the development proposal: the emotional, temporal, and spatial. Emotional expressions of objection added intensity and gave meaning to claims about the historical decline of the region and also conveyed a deep sense of the proposed building site as a lived space. Particular grounds of opposition—over fear of strangers, the fragility of a local orchid, and the pollution from mining—provide an opportunity to explore the complex nature of place meaning and community identity, ultimately leading to a conclusion that the meaning of place is always in flux. The paper argues that Simmel’s classic concept of the stranger, as the outsider who comes to stay, offers a useful analytic in understanding how the quality of proximal remoteness that prisons and other unwanted developments constitute participates in a constantly evolving sense of the local

Examining the California Cut Score: An Empirical Analysis of Minimum Competency, Public Protection, Disparate Impact, and National Standards

The selection of a minimum bar exam passing score (“cut score”) shapes the representation of racial and ethnic minorities in the legal profession and the quality of access to justice in the state. California and national policy makers have not had the benefit of detailed exam performance data that analyzes the effect of the cut score on race and ethnicity. Because policy makers consider the cut score an important public protection mechanism, this study also explored whether the selection of higher cut scores better protected the public from attorneys who do not have the minimum competence to practice law. To conduct the analysis, the study used two data sets. The first data set included 85,727 examinees who sat for 21 administrations of the CBX from 2009-18 and the race and ethnicity of each examinee. The second data set included the ABA discipline data from up to 48 U.S. jurisdictions from 2013-18 and the cut scores in each jurisdiction. Using the first data set,the study determined how the selection of a minimum cut score (1) widens or narrows the racial and ethnic impacts of the bar exam and/or (2) alters the racial and ethnic composition of new attorneys joining the legal profession. Both historical actual and simulated cut scores were analyzed. Using the second data set, this study examined a third factor: the relationship, if any, between minimum cut scores and rates of attorney discipline. This analysis determined that initial and eventual passing rates differed significantly between racial and ethnic groups, and this gap was wider at higher simulated cut scores. A simulation analysis using actual examinee scores confirmed that selecting a lower cut score would have significantly narrowed the achievement gap between Whites and racial and ethnic minorities and would have increased the number of newly admitted minority attorneys in California. For example, at 1440, the achievement gap between Whites and Blacks was 27.4 percentage points. But at a simulated cut score of 1300, the achievement gap between these two groups would have been only 14.5 percentage points. This 12.9 percentage point difference in the achievement gap at 1440 and 1300 demonstrates a disparate effect of the higher cut scores. Using the second data set about disciplinary statistics, the study determined that no relationship exists between the selection of a cut score and the number of complaints, formal charges, or disciplinary actions taken against attorneys in the jurisdictions studied. California’s recent decision to lower the cut score from 1440 to 1390 moved California from having the second-highest cut score to the fourth-highest cut score in the country. However, the report data established that at 1390 California will continue to produce significantly disparate pass rates on the basis of race and ethnicity when compared to the national norm of 1350, the New York standard of 1330, and the simulated model of 1300. This study establishes that maintaining a high cut score does not result in greater public protection as measured by disciplinary statistics but does result in excluding minorities from admission to the bar and the practice of law at rates disproportionately higher than Whites

Opioid agonist treatment and risk of death or rehospitalization following injection drug use–associated bacterial and fungal infections: A cohort study in New South Wales, Australia

Background AU Injecting-related: Pleaseconfirmthatallheadinglevelsarerepresentedcorrectly bacterial and fungal infections are associated : with significant morbidity and mortality among people who inject drugs (PWID), and they are increasing in incidence. Following hospitalization with an injecting-related infection, use of opioid agonist treatment (OAT; methadone or buprenorphine) may be associated with reduced risk of death or rehospitalization with an injecting-related infection. Methods and findings Data came from the Opioid Agonist Treatment Safety (OATS) study, an administrative linkage cohort including all people in New South Wales, Australia, who accessed OAT between July 1, 2001 and June 28, 2018. Included participants survived a hospitalization with injecting-related infections (i.e., skin and soft-tissue infection, sepsis/bacteremia, endocarditis, osteomyelitis, septic arthritis, or epidural/brain abscess). Outcomes were all-cause death and rehospitalization for injecting-related infections. OAT exposure was classified as time varying by days on or off treatment, following hospital discharge. We used separate Cox proportional hazards models to assess associations between each outcome and OAT exposure. The study included 8,943 participants (mean age 39 years, standard deviation [SD] 11 years; 34% women). The most common infections during participants’ index hospitalizations were skin and soft tissue (7,021; 79%), sepsis/bacteremia (1,207; 14%), and endocarditis (431; 5%). During median 6.56 years follow-up, 1,481 (17%) participants died; use of OAT was associated with lower hazard of death (adjusted hazard ratio [aHR] 0.63, 95% confidence interval [CI] 0.57 to 0.70). During median 3.41 years follow-up, 3,653 (41%) were rehospitalized for injecting-related infections; use of OAT was associated with lower hazard of these rehospitalizations (aHR 0.89, 95% CI 0.84 to 0.96). Study limitations include the use of routinely collected administrative data, which lacks information on other risk factors for injecting-related infections including injecting practices, injection stimulant use, housing status, and access to harm reduction services (e.g., needle exchange and supervised injecting sites); we also lacked information on OAT medication dosages. Conclusions Following hospitalizations with injection drug use–associated bacterial and fungal infections, use of OAT is associated with lower risks of death and recurrent injecting-related infections among people with opioid use disorder

Effect of incarceration and opioid agonist treatment transitions on risk of hospitalisation with injection drug use-associated bacterial infections: A self-controlled case series in New South Wales, Australia

Background: Transitional times in opioid use, such as release from prison and discontinuation of opioid agonist treatment (OAT), are associated with health harms due to changing drug consumption practices and limited access to health and social supports. Using a self-controlled (within-person) study design, we aimed to understand if these transitions increase risks of injection drug use-associated bacterial infections. Methods: We performed a self-controlled case series among a cohort of people with opioid use disorder (who had all previously accessed OAT) in New South Wales, Australia, 2001-2018. The outcome was hospitalisation with injecting-related bacterial infections. We divided participants’ observed days into time windows related to incarceration and OAT receipt. We compared hospitalization rates during focal (exposure) windows and referent (control) windows (i.e., 5-52 weeks continuously not incarcerated or continuously receiving OAT). We estimated adjusted incidence rate ratios (aIRR) using conditional logistic regression, adjusted for time-varying confounders. Results: There were 7590 participants who experienced hospitalisation with injecting-related bacterial infections (35% female; median age 38 years; 78% hospitalised with skin and soft-tissue infections). Risk for injecting-related bacterial infections was elevated for two weeks following release from prison (aIRR 1.45; 95%CI 1.22–1.72). Risk was increased during two weeks before (aIRR 1.89; 95%CI 1.59–2.25) and after (aIRR 1.91; 95%CI 1.54–2.36) discontinuation of OAT, and during two weeks before (aIRR 3.63; 95%CI 3.13–4.22) and after (aIRR 2.52; 95%CI 2.09–3.04) OAT initiation. Conclusion: Risk of injecting-related bacterial infections varies greatly within-individuals over time. Risk is raised immediately after prison release, and around initiation and discontinuation of OAT. Social contextual factors likely contribute to excess risks at transitions in incarceration and OAT exposure