Oocyte cryopreservation as an adjunct to the assisted reproductive technologies

The document attached has been archived with permission from the editor of the Medical Journal of Australia. An external link to the publisher’s copy is included. See page 2 of PDF for this item.Keith L Harrison, Michelle T Lane, Jeremy C Osborn, Christine A Kirby, Regan Jeffrey, John H Esler and David Mollo

Technical Note: Adjoint formulation of the TOMCAT atmospheric transport scheme in the Eulerian backtracking framework (RETRO-TOM)

A new methodology for the formulation of an adjoint to the transport component of the chemistry transport model TOMCAT is described and implemented in a new model, RETRO-TOM. The Eulerian backtracking method is used, allowing the forward advection scheme (Prather's second-order moments) to be efficiently exploited in the backward adjoint calculations. Prather's scheme is shown to be time symmetric, suggesting the possibility of high accuracy. To attain this accuracy, however, it is necessary to make a careful treatment of the "density inconsistency" problem inherent to offline transport models. The results are verified using a series of test experiments. These demonstrate the high accuracy of RETRO-TOM when compared with direct forward sensitivity calculations, at least for problems in which flux limiters in the advection scheme are not required. RETRO-TOM therefore combines the flexibility and stability of a "finite difference of adjoint" formulation with the accuracy of an "adjoint of finite difference" formulation

Positionally dependent ^(15)N fraction factors in the UV photolysis of N_2O determined by high resolution FTIR spectroscopy

Positionally dependent fractionation factors for the photolysis of isotopomers of N_2O in natural abundance have been determined by high resolution FTIR spectroscopy at three photolysis wavelengths. Fractionation factors show clear 15N position and photolysis wavelength dependence and are in qualitative agreement with theoretical models but are twice as large. The fractionation factors increase with photolysis wavelength from 193 to 211 nm, with the fractionation factors at 207.6 nm for ^(14)N^(15)N^916)O, ^(15)N^(14)N^(16)O and ^(14)N^(14)N^(18)O equal to −66.5±5‰,−27.1±6‰ and −49±10‰, respectively

Baseline Corticosterone in Wintering Marine Birds: Methodological Considerations and Ecological Patterns

Previous studies have related levels of plasma corticosterone (CORT) of seabirds to variation in foraging conditions during the breeding period, but it is unclear whether similar relationships between foraging conditions and baseline CORT exist during other life stages. We validated methods for identifying baseline CORT of lethally sampled birds and assessed variation in baseline CORT relative to winter habitat conditions. We collected free-living white-winged scoters (Melanitta fusca) at four wintering sites during December and February. We found increasing CORT values beyond 3 min after time since flush (the duration between initial flush and death), presumably reflecting acute stress responses. Our results demonstrate that it is possible to obtain baseline CORT from lethally sampled birds if the time from initial flush until death is measured. Our study sites varied appreciably in exposure to wind and waves, predation danger, diving depths, and the fraction of preferred foods in scoter diets. Despite these habitat differences, baseline CORT did not vary across sites or winter periods. We interpret this lack of variation as evidence that birds select wintering areas where they can successfully manage site-specific costs and maintain physiological homeostasis

A decision-making framework for restoring riparian zones degraded by invasive alien plants in South Africa

Riparian habitats in many parts of South Africa are severely degraded by invasive alien plants, especially trees. These invasions reduce water yields from catchments and affect riverine functioning and biodiversity. Initiatives are under way countrywide to clear alien plants from watercourses and surrounding catchments. Current understanding of key processes that regulate riparian functioning and define options for restoration is rudimentary. We review the impacts of riparian invasions and identify factors limiting the recovery of natural vegetation following alien clearance. We propose a framework of strategic interventions for optimizing restoration success. The framework identifies abiotic and biotic barriers to restoration at the scales of catchments and local reaches. In highly transformed catchments, interventions at the reach scale may fail if important barriers at the catchment scale are not addressed. The extent to which propagule supply and microsite conditions inhibit vegetation recovery is unknown. We also know little of the relative importance of dispersing vegetative propagules, dispersing seeds and soil-stored seed banks in vegetation dynamics, particularly after severe disturbances such as dense invasion by alien plants. The importance of geomorphological and hydrological factors in mediating recovery of riparian vegetation has not been adequately explored for all climatic areas in South Africa. More research is needed to determine the influence of different alien species and clearing treatments on the recovery of riparian vegetation. The literature strongly suggests that in highly alien-transformed catchments, the re-introduction of riparian species is required to promote recovery and suppress re-invasion. However, such interventions are unlikely to be widely implemented unless the cost: benefit ratios are favourable

Interaction between cardiac sympathetic drive and heart rate in heart failure Modulation by adrenergic receptor genotype

ObjectivesIn the present study, we aimed to evaluate the effect of adrenergic receptor polymorphisms on the response of myocardium to measured levels of cardiac adrenergic drive, and to evaluate whether polymorphisms of presynaptic adrenoceptors modified the rate of cardiac and systemic release of norepinephrine.BackgroundHeightened sympathetic activity plays an important pathophysiologic role in congestive heart failure (CHF). Recently several functionally relevant polymorphisms of the α2-, β1-, and β2-adrenoceptors have been identified, and specific genotypes have been associated with the incidence or clinical severity of CHF. These adrenoceptors are known to be located both pre-synaptically (α2and β2) and post-synaptically (β1and β2), raising the possibility that their association with clinical measures in CHF could be mediated either by modulation of the cardiac response to a given level of adrenergic drive or by altering norepinephrine release from sympathetic nerve terminals.MethodsWe determined the β1-, β2-, and α2C-adrenoceptor genotype in 60 patients with severe CHF in conjunction with measurement of cardiac and systemic sympathetic activity using the radiotracer norepinephrine spillover method.ResultsWe showed a strong relationship (r = 0.67, p < 0.001) between heart rate and the level of cardiac adrenergic drive, and heart rate for a given level of cardiac adrenergic drive was substantially greater in patients with the Arg/Arg16 β2-adrenoceptor polymorphism (p = 0.02), whereas no such relationship existed for polymorphisms of the β1-adrenoceptor. The genotype of the α2C- and β2-adrenoceptors showed no relationship to the rate of norepinephrine release from cardiac sympathetic nerves.ConclusionsFor the first time, we show that β2-adrenoceptor polymorphisms significantly influence the relationship between heart rate and cardiac adrenergic drive in CHF, but do not affect the rate of norepinephrine release from sympathetic nerve terminals