Paleolinguistics brings more light on the earliest history of the traditional Eurasian pulse crops

Traditional pulse crops such as pea, lentil, field bean, bitter vetch, chickpea and common vetch originate from Middle East, Mediterranean and Central Asia^1^. They were a part of human diets in hunter-gatherers communities^2^ and are one of the most ancient cultivated crops^3,4^. Europe has always been rich in languages^5^, with individual families still preserving common vocabularies related to agriculture^6,7^. The evidence on the early pulse history witnessed by the attested roots in diverse Eurasian proto-languages remains insufficiently clarified and its potential for supporting archaeobotanical findings is still non-assessed. Here we show that the paleolinguistic research may contribute to archaeobotany in understanding the role traditional Eurasian pulse crops had in the everyday life of ancient Europeans. It was found that the Proto-Indo-European language^8,9^ had the largest number of roots directly related to pulses, such as *arnk(')- (a leguminous plant), *bhabh- (field bean), *erəgw[h]- (a kernel of leguminous plant; pea), *ghArs- (a leguminous plant), *kek-, *k'ik'- (pea) and *lent- (lentil)^10,11,12^, numerous words subsequently related to pulses^13,14^ and borrowings from one branch to another^15^, confirming their essential place in the nutrition of Proto-Indo-Europeans^16,17,18^. It was also determined that pea was the most important among Proto-Uralic people^19,20,21^, while pea and lentil were the most significant in the agriculture of Proto-Altaic people^22,23,24^. Pea and bean were most common among Caucasians^25,26^, Basques^27,28^ and their hypothetical common forefathers^29^ and bean and lentil among the Afro-Asiatic ancestors of modern Maltese^30^. Our results demonstrate that pulses were common among the ancestors of present European nations and that paleolinguistics and its lexicological and etymological analysis may be useful in better understanding the earliest days of traditional Eurasian crops. We believe our results could be a basis for advanced multidisciplinary approach to the pulse crop domestication, involving plant scientists, archaeobotanists and linguists, and for reconstructing even earlier periods of pulse history

Effect of Mineral Nutrition on Red Clover Leaf Area Index

The legume red clover (Trifolium pratense) fixes its own nitrogen (N), but requires P and K fertilisation. There are no recent reliable data in the domestic literature, on the amounts of P and K recommended to farmers; present recommendations are often either inadequate or excessive. Red clover mineral nutrition is significantly affected by soil and weather conditions (Taylor & Quesenberry, 1996). The objective of our twoyear study was to enable rational fertiliser application in accordance with soil type and agro ecological conditions

Effect of Cutting Date on Quality of Red Clover Forage

Development stage or plant age is an important factor determining the chemical composition and quality of red clover forage (Ignjatovic et al., 2001). In early spring, young red clover plants have large leaf mass, high contents of moisture, protein and minerals and a low fibre content. In the course of the growing season, under the effects of long days and high temperatures, the plant undergoes morphological changes: leaves grow more slowly, the stem elongates, dry matter yield increases and quality drops, especially digestibility and the contents of protein and minerals

Hyperendemic Dirofilaria immitis infection in a sheltered dog population: an expanding threat in the Mediterranean region

A study on the occurrence of Dirofilaria immitis and its vectors was carried out in order to assess the prevalence of the disease in dogs in previously non-endemic areas of southern Italy. Blood samples (n = 385) and mosquitoes (n = 1540) were collected in two dog shelters and analysed by Knott's test and duplex real-time PCR, respectively. Dirofilaria immitis was the most prevalent filarioid (44.2%), while Culex pipiens was the most prevalent mosquito species (68.8%). This high prevalence of D. immitis infection confirms this location as one of the most hyperendemic foci of dirofilariosis in Europe

White lupin as a forage crop on alkaline soils

White lupin (Lupinus albus L.) is not widely known in Serbia. A small-plot trial was carried out during 2006 and 2007 at the Rimski Šancevi Experiment Field of the Institute of Field and Vegetable Crops, on a carbonated chernozem soil with a pH value in H₂O of 7.92. It included twelve white lupin accessions of diverse geographical origin from the Annual Forage Legumes Collection of the Forage Crops Department of the Institute. All accessions were sown in early March, with a crop density of 75-85 viable seeds m⁻², and were cut when main stem inflorescences were in full flower. The tallest accession was Termis (120 cm). The greatest number of main stems and first-order branches was in BG-005542 (9.0 plant⁻¹) and BG-002171 (8.7 plant⁻¹), with the former having most leaves as well (59.7 plant⁻¹). Accessions BG-005542 and LUP 261/89 had the highest green forage yields of 53.3 t ha⁻¹ and 52.9 t ha⁻¹ respectively. The average dry forage matter yield ranged from 4.0 t ha⁻¹ in BG-002173 to 8.7 t ha⁻¹ in Termis. The greatest dry matter proportion in green forage was in Termis and Siebacher Red (0.19)

Sandfly surveillance and investigation of Leishmania spp. DNA in sandflies in Kosovo

Vaselek, Slavica/0000-0002-9105-7138; OZBEL, YUSUF/0000-0001-8335-1997; Alishani, Mentor/0000-0002-6853-4792WOS: 000534417200001PubMed: 32438501In the past decade, leishmaniasis seems to be re-emerging in Balkan countries. There are serious implications that Kosovo is a visceral leishmaniasis endemic region with autochthonous transmission; nevertheless, surveillance of vectors, reservoirs or the disease is not yet established. Gaining knowledge about sandfly vector species is a prerequisite for the development of a monitoring and control plan in the future. After a long gap in research of over 70 years, sandfly studies in Kosovo were resumed in 2014. During this presence/absence study, nine sandfly species were detected: Phlebotomus papatasi, Ph. perfiliewi, Ph. tobbi, Ph. neglectus, Ph. simici, Ph. balcanicus, Ph. alexandri, Ph. mascittii and Sergentomyia minuta. Three species are new with regard to the fauna of Kosovo - Ph. alexandri, Ph. balcanicus and Ph. mascittii. Besides increased diversity, changes in the number of collected specimens and distribution range of species were recorded, with Ph. neglectus being the most dominant species with the widest distribution. Testing of randomly chosen females for Leishmania spp. DNA resulted the in detection of L. tropica in a specimen of Ph. neglectus. the presence of numerous vector species in the sandfly fauna of Kosovo pose a threat for the re-emergence of vector-borne diseases. Therefore, continuous surveillance is recommended with regular updates on vector distribution and abundance.EurNegVec COST ActionEuropean Cooperation in Science and Technology (COST) [TD1303]; VectorNet project - European Food Safety Authority (EFSA); European Centre for Disease Prevention and Control (ECDC) contract [OC/FSA/AHAW/2013/02-FWC1]; European Virus Archive goes Global project (EVAg) - European Union's Horizon 2020 research and innovation programme [653316]; Ministry of Education and Science of the Republic of Serbia [III43007, TR31084]; European Commission, Horizon 2020 Infrastructure Infravec2 projectEuropean CommissionThe authors express their gratitude to Vid Srdic who designed the map for this manuscript. the work of Slavica Vaselek, Yusuf Ozbel, Bulen Alten and Dusan Petric was carried out under the frame of EurNegVec COST Action TD1303. the work was supported by VectorNet project funded by the European Food Safety Authority (EFSA) and the European Centre for Disease Prevention and Control (ECDC) contract OC/FSA/AHAW/2013/02-FWC1; European Virus Archive goes Global project (EVAg) funded by the European Union's Horizon 2020 research and innovation programme (grant agreement No 653316); and projects III43007 and TR31084 financed by the Ministry of Education and Science of the Republic of Serbia and European Commission, Horizon 2020 Infrastructure Infravec2 project (https://infravec2.eu)

Towards conservation of the local landraces of faba bean (<em>Vicia faba</em>) in Serbia.

International audienceToday, faba bean (Vicia faba L.) in Serbia is almost an extinct crop, without official data on harvested area or production for decades. However, faba bean survived in certain parts of the country, especially along the rivers Southern and Great Morava, in the centre, and in Backa, in the north. In these parts, faba bean is cultivated mostly to suit one's own needs and sporadically is brought to green markets. It is grown as a garden crop, with sowing either in spring or during winter in order to achieve faster growth in comparison to the first schedule. First steps towards the conservation of faba bean local landraces in Serbia have been made within the multilateral project ECO-NET 18817 (2008-2009) between France, Bulgaria, Russia, Serbia and Bosnia and Herzegovina, with about 30 populations collected, and will be proceeded with within the recently launched bilateral project LEG-HIVER between Serbia and France, with multilevel characterisation and evaluation planne

Sandfly surveillance and investigation of Leishmania spp. DNA in sandflies in Kosovo

In the past decade, leishmaniasis seems to be re-emerging in Balkan countries. There are serious implications that Kosovo is a visceral leishmaniasis endemic region with autochthonous transmission; nevertheless, surveillance of vectors, reservoirs or the disease is not yet established. Gaining knowledge about sandfly vector species is a prerequisite for the development of a monitoring and control plan in the future. After a long gap in research of over 70 years, sandfly studies in Kosovo were resumed in 2014. During this presence/absence study, nine sandfly species were detected: Phlebotomus papatasi, Ph. perfiliewi, Ph. tobbi, Ph. neglectus, Ph. simici, Ph. balcanicus, Ph. alexandri, Ph. mascittii and Sergentomyia minuta. Three species are new with regard to the fauna of Kosovo - Ph. alexandri, Ph. balcanicus and Ph. mascittii. Besides increased diversity, changes in the number of collected specimens and distribution range of species were recorded, with Ph. neglectus being the most dominant species with the widest distribution. Testing of randomly chosen females for Leishmania spp. DNA resulted the in detection of L. tropica in a specimen of Ph. neglectus. The presence of numerous vector species in the sandfly fauna of Kosovo pose a threat for the re-emergence of vector-borne diseases. Therefore, continuous surveillance is recommended with regular updates on vector distribution and abundance

Genetic diversity and population structure of Culex modestus across Europe: does recent appearance in the United Kingdom reveal a tendency for geographical spread?

In mainland Europe, the mosquito species Culex modestus Ficalbi (1890) is a bridge vector for West Nile virus (WNV) from its natural bird-mosquito cycle to mammals. The present study assessed the genetic diversity of Cx. modestus, as well as related Culex species, using the mitochondrial COI DNA barcoding region and compared this with the population structure across Europe. A haplotype network was mapped to determine genealogical relationships among specimens. The intraspecific genetic diversity within individual Culex species was below 2%, whereas theinterspecificgeneticdivergencevariedfrom2.99%to13.74%.Intotal,76haplotypes were identified among 198 sequences. A median-joining network determined from 198 COI sequences identified two major lineages that were separated by at least four mutation steps. A high level of intraspecific genetic diversity was not detected in Cx. modestus in samples submitted from different European populations, which indicates that morphologically identified specimens represent a single species and not a species complex. Therefore, it is deduced that different populations of Cx. modestus will show a similar potential to transmit WNV, lending support to concerns that the population present in southeast England represents a risk of transmission to humans.The authors thank the Department for Environment and Rural Affairs (DEFRA), Scottish Government and Welsh Government (grants SV3045 and SE4113) for funding the present study. EU Framework Horizon 2020 Innovation Grant, European Virus Archive (EVAg, grant no. 653316) is also acknowledged for providing funds for sequencing. Ignacio Ruiz-Arrondo thanks the European Regional Development Fund (ERDF) for funding.info:eu-repo/semantics/publishedVersio