Membrane fluidity and temperature sensing are coupled via circuitry comprised of Ole1, Rsp5, and Hsf1 in Candida albicans

Peer reviewedPublisher PD

Surviving the heat of the moment : a fungal pathogens perspective

Peer reviewedPublisher PD

Estimating the Explosion Time of Core-Collapse Supernovae from Their Optical Light Curves

Core-collapse supernovae are among the prime candidate sources of high energy neutrinos. Accordingly, the IceCube collaboration has started a program to search for such a signal. IceCube operates an online search for neutrino bursts, forwarding the directions of candidate events to a network of optical telescopes for immediate follow-up observations. If a supernova is identified from the optical observations, in addition to a directional coincidence a temporal photon-neutrino coincidence also needs to be established. To achieve this, we present a method for estimating the supernova explosion time from its light curve using a simple model. We test the model with supernova light curve data from SN1987A, SN2006aj and SN2008D and show that the explosion times can be determined with an accuracy of better than a few hours.Comment: Version accepted for publication by Astroparticle Physics; 13 pages, 5 figure

Remote determination of the velocity index and mean streamwise velocity profiles

Author Posting. © American Geophysical Union, 2017. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Water Resources Research 53 (2017): 7521–7535, doi:10.1002/2017WR020504.When determining volumetric discharge from surface measurements of currents in a river or open channel, the velocity index is typically used to convert surface velocities to depth-averaged velocities. The velocity index is given by, inline image, where Ub is the depth-averaged velocity and Usurf is the local surface velocity. The USGS (United States Geological Survey) standard value for this coefficient, k = 0.85, was determined from a series of laboratory experiments and has been widely used in the field and in laboratory measurements of volumetric discharge despite evidence that the velocity index is site-specific. Numerous studies have documented that the velocity index varies with Reynolds number, flow depth, and relative bed roughness and with the presence of secondary flows. A remote method of determining depth-averaged velocity and hence the velocity index is developed here. The technique leverages the findings of Johnson and Cowen (2017) and permits remote determination of the velocity power-law exponent thereby, enabling remote prediction of the vertical structure of the mean streamwise velocity, the depth-averaged velocity, and the velocity index.National Institutes for Water Resources Grant Number: 2012NY189G2018-03-0

Movements and spawning of white marlin (Tetrapturus albidus) and blue marlin (Makaira nigricans) off Punta Cana, Dominican Republic

With a focus on white marlin (Tetrapturus albidus), a concurrent electronic tagging and larval sampling effort was conducted in the vicinity of Mona Passage (off southeast Hispaniola), Dominican Republic, during April and May 2003. Objectives were 1) to characterize the horizontal and vertical movement of adults captured from the area by using pop-up satellite archival tags (PSATs); and 2) by means of larval sampling, to investigate whether fish were reproducing. Trolling from a sportfishing vessel yielded eight adult white marlin and one blue marlin (Makaira nigricans); PSAT tags were deployed on all but one of these individuals. The exception was a female white marlin that was unsuitable for tagging because of injury; the reproductive state of its ovaries was examined histologically. Seven of the PSATs reported data summaries for water depth, temperature, and light levels measured every minute for periods ranging from 28 to 40 days. Displacement of marlin from the location of release to the point of tag pop-up ranged from 3l.6 to 267.7 nautical miles (nmi) and a mean displacement was 3.4 nmi per day for white marlin. White and blue marlin mean daily displacements appeared constrained compared to the results of other marlin PSAT tagging studies. White marlin ovarian sections contained postovulatory follicles and final maturation-stage oocytes, which indicated recent and imminent spawning. Neuston tows (n=23) yielded 18 istiophorid larvae: eight were white marlin, four were blue marlin, and six could not be identified to species. We speculate that the constrained movement patterns of adults may be linked to reproductive activity for both marlin species, and, if true, these movement patterns may have several implications for management. Protection of the potentially important white marlin spawning ground near Mona Passage seems warranted, at least until further studies can be conducted on the temporal and spatial extent of reproduction and associated adult movement

Review—Organic Materials for Thermoelectric Energy Generation

Organic semiconductor materials have been promising alternatives to their inorganic counterparts in several electronic applications such as solar cells, light emitting diodes, field effect transistors as well as thermoelectric generators. Their low cost, light weight and flexibility make them appealing in future applications such as foldable electronics and wearable circuits using printing techniques. In this report, we present a mini-review on the organic materials that have been used for thermoelectric energy generation

Swift follow-up of IceCube triggers, and implications for the Advanced-LIGO era

Between 2011 March and 2014 August Swift responded to 20 triggers from the IceCube neutrino observatory, observing the IceCube 50% confidence error circle in X-rays, typically within 5 hours of the trigger. No confirmed counterpart has been detected. We describe the Swift follow up strategy and data analysis and present the results of the campaign. We discuss the challenges of distinguishing the X-ray counterpart to a neutrino trigger from serendipitous uncatalogued X-ray sources in the error circle, and consider the implications of our results for future strategies for multi-messenger astronomy, with particular reference to the follow up of gravitational wave triggers from the advanced-era detectors.Comment: Accepted for publication in MNRAS. 18 pages, including 8 figures and 4 tables; two of which are landscape-oriente

Hsp90 governs dispersion and drug resistance of fungal biofilms

Fungal biofilms are a major cause of human mortality and are recalcitrant to most treatments due to intrinsic drug resistance. These complex communities of multiple cell types form on indwelling medical devices and their eradication often requires surgical removal of infected devices. Here we implicate the molecular chaperone Hsp90 as a key regulator of biofilm dispersion and drug resistance. We previously established that in the leading human fungal pathogen, Candida albicans, Hsp90 enables the emergence and maintenance of drug resistance in planktonic conditions by stabilizing the protein phosphatase calcineurin and MAPK Mkc1. Hsp90 also regulates temperature-dependent C. albicans morphogenesis through repression of cAMP-PKA signalling. Here we demonstrate that genetic depletion of Hsp90 reduced C. albicans biofilm growth and maturation in vitro and impaired dispersal of biofilm cells. Further, compromising Hsp90 function in vitro abrogated resistance of C. albicans biofilms to the most widely deployed class of antifungal drugs, the azoles. Depletion of Hsp90 led to reduction of calcineurin and Mkc1 in planktonic but not biofilm conditions, suggesting that Hsp90 regulates drug resistance through different mechanisms in these distinct cellular states. Reduction of Hsp90 levels led to a marked decrease in matrix glucan levels, providing a compelling mechanism through which Hsp90 might regulate biofilm azole resistance. Impairment of Hsp90 function genetically or pharmacologically transformed fluconazole from ineffectual to highly effective in eradicating biofilms in a rat venous catheter infection model. Finally, inhibition of Hsp90 reduced resistance of biofilms of the most lethal mould, Aspergillus fumigatus, to the newest class of antifungals to reach the clinic, the echinocandins. Thus, we establish a novel mechanism regulating biofilm drug resistance and dispersion and that targeting Hsp90 provides a much-needed strategy for improving clinical outcome in the treatment of biofilm infections