Elaboration et évaluation d'algorithmes de dépistage des MST chez la femme enceinte à Libreville, Gabon

La lutte contre les MST constitue une priorité de santé publique des pays en développement de par leurs complications propres, en particulier chez la femme, et de par leur rôle facilitant la transmission du VIH. Une des stratégies de lutte contre les MST est le diagnostic et le traitement précoces de ces infections. Les MST, en particulier les infections à gonocoques et à #Chlamydiae trachomatis$sont d'un diagnostic difficile chez la femme en l'absence d'examens complémentaires, ceux-ci faisant souvent défaut au niveau des structures de soins de santé primaire. Dans ce contexte, l'approche syndromique, basée sur la prise en compte de signes et symptômes, peut permettre de standardiser et d'améliorer la prise en charge des patientes. Une étude de prévalence et de facteurs de risque des MST, effectuées chez 192 femmes enceintes consultant en PMI à Libreville (Gabon), en septembre 1993, a confirmé la prévalence des MST dans cette population (13,5$). L'évaluation de différentes stratégies diagnostiques et d'algorithmes montre que, quel que soit le niveau d'examen (données d'interrogatoire, examen clinique simple, examen au spéculum), l'utilisation de scores intégrant des facteurs de risque, des symptômes et des signes cliniques est plus performante que les algorithmes hiérarchiques. Ces scores présentent des sensibilités et des spécificités élevées et sont d'une mise en oeuvre facile. Leur application pourrait donc permettre le dépistage efficace des MST et éviter, ainsi, une grande partie des complications maternelles et infantiles. (Résumé d'auteur

Modèles ecologiques pour l'extrapolation des effets écotoxicologiques enregistrés lors de biotests in situ cheZ Gammarus

[Departement_IRSTEA]Eaux [TR1_IRSTEA]BELCAInternational audienceEvaluating the effects of chemical contamination on populations and ecological communities still constitutes a challenging necessity in environmental management. However the toxic effects of contaminants are commonly measured by means of organism-level responses. Linking such effects measures with ecological models is a promising way to apprehend population-level impacts. In this way, population models are currently increasingly used in predictive risk assessment procedures, but their use in environmental diagnostic framework remains limited due to their lack of ecological realism. The present study with the crustacean amphipod Gammarus fossarum, a sentinel species in freshwater monitoring, combines a dual field and laboratory experimental approach with a population modelling framework. In this way, we developed an ecologically-relevant periodic matrix population model for Gammarus. This model allowed us to capture the population dynamics in the field, and to understand the particular pattern of demographic sensitivities induced by Gammarus life-history phenology. The model we developed provided a robust population-level assessment of in situ-based effects measures recorded during a biomonitoring program on a French watershed impacted by past mining activities. Thus, our study illustrates the potential of population modelling when seeking to decipher the role of environmental toxic contamination in ecological perturbations

HIV-1 Protease and Reverse Transcriptase Control the Architecture of Their Nucleocapsid Partner

The HIV-1 nucleocapsid is formed during protease (PR)-directed viral maturation, and is transformed into pre-integration complexes following reverse transcription in the cytoplasm of the infected cell. Here, we report a detailed transmission electron microscopy analysis of the impact of HIV-1 PR and reverse transcriptase (RT) on nucleocapsid plasticity, using in vitro reconstitutions. After binding to nucleic acids, NCp15, a proteolytic intermediate of nucleocapsid protein (NC), was processed at its C-terminus by PR, yielding premature NC (NCp9) followed by mature NC (NCp7), through the consecutive removal of p6 and p1. This allowed NC co-aggregation with its single-stranded nucleic-acid substrate. Examination of these co-aggregates for the ability of RT to catalyse reverse transcription showed an effective synthesis of double-stranded DNA that, remarkably, escaped from the aggregates more efficiently with NCp7 than with NCp9. These data offer a compelling explanation for results from previous virological studies that focused on i) Gag processing leading to nucleocapsid condensation, and ii) the disappearance of NCp7 from the HIV-1 pre-integration complexes. We propose that HIV-1 PR and RT, by controlling the nucleocapsid architecture during the steps of condensation and dismantling, engage in a successive nucleoprotein-remodelling process that spatiotemporally coordinates the pre-integration steps of HIV-1. Finally we suggest that nucleoprotein remodelling mechanisms are common features developed by mobile genetic elements to ensure successful replication

Islet Endothelial Activation and Oxidative Stress Gene Expression Is Reduced by IL-1Ra Treatment in the Type 2 Diabetic GK Rat

Inflammation followed by fibrosis is a component of islet dysfunction in both rodent and human type 2 diabetes. Because islet inflammation may originate from endothelial cells, we assessed the expression of selected genes involved in endothelial cell activation in islets from a spontaneous model of type 2 diabetes, the Goto-Kakizaki (GK) rat. We also examined islet endotheliuml/oxidative stress (OS)/inflammation-related gene expression, islet vascularization and fibrosis after treatment with the interleukin-1 (IL-1) receptor antagonist (IL-1Ra)

Bushfire in Madagascar: natural hazard, useful tool, and change agent

Bushfire does not usually figure on lists of natural hazards in Madagascar, despite being damaging hazard on the island. This chapter argues that the lack of attention to fire comes from the fact that fire is multivalent, ambiguous, and flexible, being simultaneously an occasional 'hazard' for thatch-roof huts or crop fields, a 'useful tool' that farmers and pastoralists use to manage vegetation, and a major transformational force, or 'change agent', that damages land and forest. The chapter builds on the concept of pyric phases, identifying six important pyric phases in which fire serves, or is perceived to serve, different roles as hazard, tool, or change agent

Utilisation, en Guadeloupe, des techniques hormonales de maîtrise des cycles

International audienc

Technique permettant d'améliorer la fertilité des femelles françaises frisonnes pie noire (FFPN) en climat tropical. Influence sur l'évolution de la progestérone plasmatique

International audienc

Induction d'une ovulation post-partum chez la vache créole à l'aide d'une séparation temporaire du veau et de l'utilisation préalable d'un implant de Norgestomet

National audienc

Induction d'une ovulation post-partum chez la vache creole a l'aide d'une separation temporaire du veau et de l'utilisation prealable d'un implant de Norgestomet

National audienc