Vegetation Effects on Fish Distribution in Impounded Salt Marshes

We compared the density and biomass of resident fish in vegetated and unvegetated flooded habitats of impounded salt marshes in the northern Indian River Lagoon (IRL) Estuary of east-central Florida. A 1-m2 throw trap was used to sample fish in randomly located, paired sample plots (n = 198 pairs) over 5 seasons in 7 impoundments. We collected a total of 15 fish taxa, and 88% of the fishes we identified from the samples belonged to three species: Cyprinodon variegatus (Sheepshead Minnow), Gambusia holbrooki (Eastern Mosquitofish), and Poecilia latipinna (Sailfin Molly). Vegetated habitat usually had higher density and biomass of fish. Mean fish density (and 95% confidence interval) for vegetated and unvegetated sites were 8.2 (6.7–9.9) and 2.0 (1.6–2.4) individuals m-2, respectively; mean biomass (and 95% confidence interval) for vegetated and unvegetated sites were 3.0 (2.5–3.7) and 1.1 (0.9–1.4) g m-2, respectively. We confirmed previous findings that impounded salt marshes of the northern IRL Estuary produce a high standing stock of resident fishes. Seasonal patterns of abundance were consistent with fish moving between vegetated and unvegetated habitat as water levels changed in the estuary. Differences in density, mean size, and species composition of resident fishes between vegetated and unvegetated habitats have important implications for movement of biomass and nutrients out of salt marsh by piscivores (e.g., wading birds and fishes) via a trophic relay

Scale-Dependent Habitat Selection of Nesting Great Egrets and Snowy Egrets

Foraging habitat selection of nesting Great Egrets ( Ardea alba ) and Snowy Egrets ( Egretta thula ) was investigated within an estuary with extensive impounded salt marsh habitat. Using a geographic information system, available habitat was partitioned into concentric bands at five, ten, and 15 km radius from nesting colonies to assess the relative effects of habitat composition and distance on habitat selection. Snowy Egrets were more likely than Great Egrets to depart colonies and travel to foraging sites in groups, but both species usually arrived at sites that were occupied by other wading birds. Mean flight distances were 6.2 km (SE = 0.4, N = 28, range 1.8-10.7 km) for Great Egrets and 4.7 km (SE = 0.48, N = 31, range 0.7-12.5 km) for Snowy Egrets. At the broadest spatial scale both species used impounded (mostly salt marsh) and estuarine edge habitat more than expected based on availability while avoiding unimpounded (mostly fresh water wetland) habitat. At more local scales habitat use matched availability. Interpretation of habitat preference differed with the types of habitat that were included and the maximum distance that habitat was considered available. These results illustrate that caution is needed when interpreting the results of habitat preference studies when individuals are constrained in their choice of habitats, such as for central place foragers

Co-located wave and offshore wind farms: A preliminary approach to the shadow effect

In recent years, with the consolidation of offshore wind technology and the progress carried out for wave energy technology, the option of combine both technologies has arisen. This combination rest mainly in two main reasons: in one hand, to increase the sustainability of both energies by means of a more rational harnessing of the natural resources; in the other hand, to reduce the costs of both technologies by sharing some of the most important costs of an offshore project. In addition to these two powerful reasons there are a number of technology synergies between wave and wind systems which makes their combination even more suitable. Co-located projects are one of the alternatives to combine wave-wind systems, and it is specially for these project were so-called shadow effect synergy becomes meaningful. In particular, this paper deals with the co-location of Wave Energy Conversion (WEC) technologies into a conventional offshore wind farm. More specifically, an overtopping type of WEC technology was considered in this work to study the effects of its co-location with a conventional offshore wind park. This study aims to give a preliminary approach to the shadow effect and its implications for both wave and offshore wind energies

CO-LOCATED WAVE AND OFFSHORE WIND FARMS: A PRELIMINARY CASE STUDY OF AN HYBRID ARRAY

In recent years, with the consolidation of offshore wind technology and the progress carried out for wave energy technology, the option of co-locate both technologies at the same marine area has arisen. Co-located projects are a combined solution to tackle the shared challenge of reducing technology costs or a more sustainable use of the natural resources. In particular, this paper deals with the co-location of Wave Energy Conversion (WEC) technologies into a conventional offshore wind farm. More specifically, an overtopping type of WEC technology was considered in this work to study the effects of its co-location with a conventional offshore wind park

A review of combined wave and offshore wind energy

The sustainable development of the offshore wind and wave energy sectors requires optimising the exploitation of the resources, and it is in relation to this and the shared challenge for both industries to reduce their costs that the option of integrating offshore wind and wave energy arose during the past decade. The relevant aspects of this integration are addressed in this work: the synergies between offshore wind and wave energy, the different options for combining wave and offshore wind energy, and the technological aspects. Because of the novelty of combined wave and offshore wind systems, a comprehensive classification was lacking. This is presented in this work based on the degree of integration between the technologies, and the type of substructure. This classification forms the basis for the review of the different concepts. This review is complemented with specific sections on the state of the art of two particularly challenging aspects, namely the substructures and the wave energy conversion

Green Fluorescent Protein in the sea urchin: new experimental approaches to transcriptional regulatory analysis in embryos and larvae

The use of Green Fluorescent Protein (GFP) as a reporter for expression transgenes opens the way to several new experimental strategies for the study of gene regulation in sea urchin development. A GFP coding sequence was associated with three different previously studied cis-regulatory systems, viz those of the SM50 gene, expressed in skeletogenic mesenchyme, the CyIIa gene, expressed in archenteron, skeletogenic and secondary mesenchyme, and the Endo16 gene, expressed in vegetal plate, archenteron and midgut. We demonstrate that the sensitivity with which expression can be detected is equal to or greater than that of whole-mount in situ hybridization applied to detection of CAT mRNA synthesized under the control of the same cis-regulatory systems. However, in addition to the important feature that it can be visualized nondestructively in living embryos, GFP has other advantages. First, it freely diffuses even within fine cytoplasmic cables, and thus reveals connections between cells, which in sea urchin embryos is particularly useful for observations on regulatory systems that operate in the syncytial skeletogenic mesenchyme. Second, GFP expression can be dramatically visualized in postembryonic larval tissues. This brings postembryonic larval developmental processes for the first time within the easy range of gene transfer analyses. Third, GFP permits identification and segregation of embryos in which the clonal incorporation of injected DNA has occurred in any particular desired region of the embryo. Thus, we show explicitly that, as expected, GFP transgenes are incorporated in the same nuclei together with other transgenes with which they are co-injected

Deciphering interplay between Salmonella invasion effectors

Bacterial pathogens have evolved a specialized type III secretion system (T3SS) to translocate virulence effector proteins directly into eukaryotic target cells. Salmonellae deploy effectors that trigger localized actin reorganization to force their own entry into non-phagocytic host cells. Six effectors (SipC, SipA, SopE/2, SopB, SptP) can individually manipulate actin dynamics at the plasma membrane, which acts as a ‘signaling hub’ during Salmonella invasion. The extent of crosstalk between these spatially coincident effectors remains unknown. Here we describe trans and cis binary entry effector interplay (BENEFIT) screens that systematically examine functional associations between effectors following their delivery into the host cell. The results reveal extensive ordered synergistic and antagonistic relationships and their relative potency, and illuminate an unexpectedly sophisticated signaling network evolved through longstanding pathogen–host interaction

The effect of after-school music programs in at-risk students\u27 self-esteem and social skills in San Juan, Puerto Rico

The purpose of this study was to examine and expand upon the role the Programas de Orquestas Sinfonicas Juveniles (POSJU) experiences play in self-esteem and social skills. The research took place in Felipe Gutierrez y Espinoza School, one of ten POSJU centers, located in the San Juan, Puerto Rico. Thirty-eight students (N=38) aged 7 to 17 participated in this study. Participants rated their self-esteem and social skills using the Rosenberg Self-Esteem Scale (RSES) and the Social Skills Competence Checklist (SSCC). No significant differences between pre- and post-evaluations on RSES and. SSCC were found. For additional information, teachers evaluated all participants using the Teacher Student Report (TSR). Significant differences were found across the construct of social skills, but not self-esteem. Information regarding the POSJU after school program was collected from parents through a Parent Questionnaire Report (PQR). Overall, parents’ responses towards the program show satisfaction with POSJU

HDAC7 Is a Repressor of Myeloid Genes Whose Downregulation Is Required for Transdifferentiation of Pre-B Cells into Macrophages

B lymphopoiesis is the result of several cell-commitment, lineage-choice, and differentiation processes. Every differentiation step is characterized by the activation of a new, lineage-specific, genetic program and the extinction of the previous one. To date, the central role of specific transcription factors in positively regulating these distinct differentiation processes to acquire a B cell-specific genetic program is well established. However, the existence of specific transcriptional repressors responsible for the silencing of lineage inappropriate genes remains elusive. Here we addressed the molecular mechanism behind repression of non-lymphoid genes in B cells. We report that the histone deacetylase HDAC7 was highly expressed in pre-B cells but dramatically down-regulated during cellular lineage conversion to macrophages. Microarray analysis demonstrated that HDAC7 re-expression interfered with the acquisition of the gene transcriptional program characteristic of macrophages during cell transdifferentiation; the presence of HDAC7 blocked the induction of key genes for macrophage function, such as immune, inflammatory, and defense response, cellular response to infections, positive regulation of cytokines production, and phagocytosis. Moreover, re-introduction of HDAC7 suppressed crucial functions of macrophages, such as the ability to phagocytose bacteria and to respond to endotoxin by expressing major pro-inflammatory cytokines. To gain insight into the molecular mechanisms mediating HDAC7 repression in pre-B cells, we undertook co-immunoprecipitation and chromatin immunoprecipitation experimental approaches. We found that HDAC7 specifically interacted with the transcription factor MEF2C in pre-B cells and was recruited to MEF2 binding sites located at the promoters of genes critical for macrophage function. Thus, in B cells HDAC7 is a transcriptional repressor of undesirable genes. Our findings uncover a novel role for HDAC7 in maintaining the identity of a particular cell type by silencing lineage-inappropriate genes