Antimicrobial activity of rhizomes of Ferulago trachycarpa Boiss. and bioguided isolation of active coumarin constituents

Ferulago trachycarpa (Apiaceae) is a plant used traditionally for its sedative, digestive, carminative and aphrodisiac properties with distribution in West, Southwest and South Anatolian part of Turkey. In this study the antimicrobial activities of fractionally n-hexane, dichloromethane, methanol and only methanol extracts from rhizomes of F. trachycarpa were screened against Stapylococcus aureus ATCC 6538, S. epidermidis ATCC 12228, Escherichia coli ATCC 8739, Kiebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 14153, Enterococcus feacalis ATCC 29212 bacterial strains and fungal strains such as Candida albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019 by microdilution method. All extracts have been shown to possess antimicrobial activities against bacteria and fungal strains and according to the antimicrobial results, the isolation of the active constituents was made from the most active n-hexane and dichloromethane extracts. So, four pure compounds are known as coumarin derivatives, crenulatin (6-formyl-7-methoxycoumarin), suberosin (7-methoxy-6-prenylcoumarin), marmesin senecioate ((-)-prantschimgin) as dihydrofuranocoumarin derivative and ulopterol [6- (2', 3'-dihydroxy-3'-methylbuty1)-7-methoxy-coumarin] were isolated. Crenulatin (6-formyl-7-methoxycoumarin), suberosin (7-methoxy-6-prenylcoumarin), marmesin senecioate ((-)-prantschimgin) which are pure compounds demonstrated antifungal activity with 625 mg/L MIC against C. albicans and antibacterial activity with 1250 mg/L MIC against S. aureus (MRSA). These results indicate that extracts and pure compounds obtained from Ferulago trachycarpa could be a potential for pharmaceutical products which have antimicrobial activity

Antimicrobial activities of medicinal plants containing phenolic compounds

Background: Medicinal plants have been used to treat diseases for centuries. They are important sources in terms of their pharmacological effects and also have many microbial agents. Recently, the development of drug resistance has begun to spread in human pathogens against used antibiotics and this has led to new research for novel antimicrobial substances from natural products containing plants. Objective: The purpose of this review is to determine the antimicrobial activity of pure phenolic compounds isolated from medicinal plants, to evaluate how molecular structures of these compounds affect the activity, and to provide to the readers a source for future studies on natural antimicrobial agents. Methods: Relevant information was gathered from scientific engines and databases (Google Scholar, Web of Science, Scifinder, Science Direct, Scopus, Wiley Online Library, PubMed, Taylor & Fran-cis online) using different keywords. Antimicrobial activity research was selected especially on pure phenolic compounds. Results: Numerous phenolic compounds were isolated from plants/plant extracts and were shown to prevent the growth of bacteria and fungi and their Minimum Inhibition Concentration (MIC) and inhibition zone values were given in detail. Conclusion: This review revealed that phenolic compounds found in medicinal plants have different antimicrobial activity according to their changes in the molecular structure. © 2020 Bentham Science Publishers

Phytochemical evaluation of the Ferulago genus and the pharmacological activities of its coumarin constituents

The genus Ferulago W. Koch is a member of the Apiaceae (Umbelliferae) family and consists of 49 species throughout the world. In traditional medicine, Ferulago species have been utilized as sedatives, carminatives, aphrodisiacs, for increasing milk secretion and for the treatment of bronchitis, skin diseases, and eye diseases. The authors aimed to evaluate the phytochemical constituents of Ferulago species and the pharmacological activities of its coumarins. In this review, literature searches were performed using scientific search engines and databases (Google Scholar, SciFinder, Science Direct, Scopus, PubMed.) for papers published in the past until June 2019. The molecular structures of all isolated compounds are shown in Tables 2, 3, and 4, and the main constituents of the essential oil (Table 5) are indicated with the identified number, characterization, and extraction method. Additionally, the pharmacological activities of the coumarins isolated from the Ferulago genus were investigated. The results demonstrated that coumarins have various pharmacological activities such as antimicrobial, antioxidant, antiproliferative, and anticholinesterase activities. Therefore this review may be considered to be an important scientific resource for future research. © 2020 Elsevier Gmb

LC-MS/MS analysis and biological activities of endemic Achillea sieheana Stapf from Türkiye

© 2022 Marmara University Press.In this study, in vitro ABTS, DPPH, 5-lipoxygenase, and α-glucosidase inhibitory activities of n-hexane (ASH), dichloromethane (ASD), ethyl acetate (ASE), and methanol (ASM) extracts from the aerial parts of endemic A. sieheana were assayed for the first time. The phytochemical content of ASE, the most active extract, has been first determined by LC-MS/MS. Also, the total amount of phenolic compounds of extracts were calculated. ASE demonstrated significant antioxidant activity with IC50 values of 0.096 and 0.156 mg/mL for ABTS and DPPH, respectively. ASD and ASE with IC50 values of 0.045 and 0.089 mg/mL exhibited significant anti-inflammatory activity. ASE showed moderate α-glucosidase inhibitory activity with an IC50 value of 0.774 mg/mL. Also, the total phenolic content was found to be highest in the ASE. Feruloylquinic acid, luteolin, luteolin glucoside, isorhamnetin, isovitexin, methoxyflavonoid like chrysoeriol and its glycoside derivative in the ASE were detected by LC-MS/MS. The results demonstrated that ASE had significant anti-inflammatory, antioxidant and moderate α-glucosidase inhibitory potential

Antimicrobial Activities of Medicinal Plants Containing Phenolic Compounds

Background: Medicinal plants have been used to treat diseases for centuries. They are important sources in terms of their pharmacological effects and also have many microbial agents. Recently, the development of drug resistance has begun to spread in human pathogens against used antibiotics and this has led to new research for novel antimicrobial substances from natural products containing plants

Characterization of the Biological Activity and Phenolics in Achillea lycaonica

The purpose of this study was to comparatively evaluate for the first time the antioxidant, antimicrobial, and antiurease effects of A. lycaonica extracts obtained using three extraction methods and five solvents followed by identification of the phenolic compounds obtained by Soxhlet extraction with ethyl acetate and maceration with chloroform that showed the highest total phenolic and flavonoid contents with antioxidant and antiurease activity. The phenolic compounds were identified to be chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, salicylic acid, luteolin, quercetin, naringenin, apigenin, and 8-hydroxy- salvigenin. Among the solvent extraction procedures, Soxhlet approach was found to have higher recoveries than other approaches. The results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays showed that the 1: 1 ethanol-water Soxhlet, ethanol ultrasonic bath, and ethyl acetate Soxhlet extraction methods had the highest DPPH antioxidant activities. The ethyl acetate Soxhlet extraction method showed the highest 2, 2'-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) antioxidant activity. In addition, the chloroform maceration extraction method exhibited the highest antiurease activity. Therefore, 1: 1 Soxhlet ethanol-water, ultrasonic bath ethanol, and Soxhlet ethyl acetate extracts may be used in medicine and food production as natural antioxidant sources. In addition, maceration with chloroform may provide a potential antiurease agent

Antimicrobial activity of rhizomes of Ferulago trachycarpa Boiss. and bioguided isolation of active coumarin constituents

Ferulago trachycarpa (Apiaceae) is a plant used traditionally for its sedative, digestive, carminative and aphrodisiac properties with distribution in West, Southwest and South Anatolian part of Turkey. In this study the antimicrobial activities of fractionally n-hexane, dichloromethane, methanol and only methanol extracts from rhizomes of F. trachycarpa were screened against Stapylococcus aureus ATCC 6538, S. epidermidis ATCC 12228, Escherichia coli ATCC 8739, Kiebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 14153, Enterococcus feacalis ATCC 29212 bacterial strains and fungal strains such as Candida albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019 by microdilution method. All extracts have been shown to possess antimicrobial activities against bacteria and fungal strains and according to the antimicrobial results, the isolation of the active constituents was made from the most active n-hexane and dichloromethane extracts. So, four pure compounds are known as coumarin derivatives, crenulatin (6-formyl-7-methoxycoumarin), suberosin (7-methoxy-6-prenylcoumarin), marmesin senecioate ((-)-prantschimgin) as dihydrofuranocoumarin derivative and ulopterol [6- (2', 3'-dihydroxy-3'-methylbuty1)-7-methoxy-coumarin] were isolated. Crenulatin (6-formyl-7-methoxycoumarin), suberosin (7-methoxy-6-prenylcoumarin), marmesin senecioate ((-)-prantschimgin) which are pure compounds demonstrated antifungal activity with 625 mg/L MIC against C. albicans and antibacterial activity with 1250 mg/L MIC against S. aureus (MRSA). These results indicate that extracts and pure compounds obtained from Ferulago trachycarpa could be a potential for pharmaceutical products which have antimicrobial activity

Characterization of the Biological Activity and Phenolics in Achillea lycaonica

The purpose of this study was to comparatively evaluate for the first time the antioxidant, antimicrobial, and antiurease effects of A. lycaonica extracts obtained using three extraction methods and five solvents followed by identification of the phenolic compounds obtained by Soxhlet extraction with ethyl acetate and maceration with chloroform that showed the highest total phenolic and flavonoid contents with antioxidant and antiurease activity. The phenolic compounds were identified to be chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, salicylic acid, luteolin, quercetin, naringenin, apigenin, and 8-hydroxy- salvigenin. Among the solvent extraction procedures, Soxhlet approach was found to have higher recoveries than other approaches. The results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays showed that the 1: 1 ethanol-water Soxhlet, ethanol ultrasonic bath, and ethyl acetate Soxhlet extraction methods had the highest DPPH antioxidant activities. The ethyl acetate Soxhlet extraction method showed the highest 2, 2'-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) antioxidant activity. In addition, the chloroform maceration extraction method exhibited the highest antiurease activity. Therefore, 1: 1 Soxhlet ethanol-water, ultrasonic bath ethanol, and Soxhlet ethyl acetate extracts may be used in medicine and food production as natural antioxidant sources. In addition, maceration with chloroform may provide a potential antiurease agent