155 research outputs found
Mapping vesicle shapes into the phase diagram: A comparison of experiment and theory
Phase-contrast microscopy is used to monitor the shapes of micron-scale
fluid-phase phospholipid-bilayer vesicles in aqueous solution. At fixed
temperature, each vesicle undergoes thermal shape fluctuations. We are able
experimentally to characterize the thermal shape ensemble by digitizing the
vesicle outline in real time and storing the time-sequence of images. Analysis
of this ensemble using the area-difference-elasticity (ADE) model of vesicle
shapes allows us to associate (map) each time-sequence to a point in the
zero-temperature (shape) phase diagram. Changing the laboratory temperature
modifies the control parameters (area, volume, etc.) of each vesicle, so it
sweeps out a trajectory across the theoretical phase diagram. It is a
nontrivial test of the ADE model to check that these trajectories remain
confined to regions of the phase diagram where the corresponding shapes are
locally stable. In particular, we study the thermal trajectories of three
prolate vesicles which, upon heating, experienced a mechanical instability
leading to budding. We verify that the position of the observed instability and
the geometry of the budded shape are in reasonable accord with the theoretical
predictions. The inability of previous experiments to detect the ``hidden''
control parameters (relaxed area difference and spontaneous curvature) make
this the first direct quantitative confrontation between vesicle-shape theory
and experiment.Comment: submitted to PRE, LaTeX, 26 pages, 11 ps-fi
Gravity-Induced Shape Transformations of Vesicles
We theoretically study the behavior of vesicles filled with a liquid of
higher density than the surrounding medium, a technique frequently used in
experiments. In the presence of gravity, these vesicles sink to the bottom of
the container, and eventually adhere even on non - attractive substrates. The
strong size-dependence of the gravitational energy makes large parts of the
phase diagram accessible to experiments even for small density differences. For
relatively large volume, non-axisymmetric bound shapes are explicitly
calculated and shown to be stable. Osmotic deflation of such a vesicle leads
back to axisymmetric shapes, and, finally, to a collapsed state of the vesicle.Comment: 11 pages, RevTeX, 3 Postscript figures uuencode
Giant vesicles at the prolate-oblate transition: A macroscopic bistable system
Giant phospholipid vesicles are shown to exhibit thermally activated
transitions between a prolate and an oblate shape on a time scale of several
seconds. From the fluctuating contour of such a vesicle we extract ellipticity
as an effective reaction coordinate whose temporal probability distribution is
bimodal. We then reconstruct the effective potential from which we derive an
activation energy of the order of in agreement with theoretical
calculations. The dynamics of this transition is well described within a
Kramers model of overdamped diffusion in a bistable potential. Thus, this
system can serve as a model for macroscopic bistability.Comment: 10 pages, LaTeX, epsfig, 4 eps figures included, to appear in
Europhys. Let
ROSAT HRI observations of Centaurus A
We present results from a sensitive high-resolution X-ray observation of the
nearby active galaxy Centaurus A (NGC 5128) with the ROSAT HRI. The 65~ksec
X-ray image clearly distinguishes different components of the X-ray emission
from Cen A: the nucleus and the jet, the diffuse galaxy halo, and a number of
individual sources associated with the galaxy. The luminosity of the nucleus
increased by a factor of two compared to an earlier ROSAT observation in 1990.
The high spatial resolution of the ROSAT HRI shows that most of the knots in
the jet are extended both along and perpendicular to the jet axis. We report
the detection of a new X-ray feature, at the opposite side of the X-ray jet
which is probably due to compression of hot interstellar gas by the expanding
southwestern inner radio lobe.Comment: To be published in Astrophys. Journal Letters. 4 pages, 3 plate
On Shape Transformations and Shape Fluctuations of Cellular Compartments and Vesicles
We discuss the shape formation and shape transitions of simple bilayer vesicles in context with their role in biology. In the first part several classes of shape changes of vesicles of one lipid component are described and it is shown that these can be explained in terms of the bending energy concept in particular augmented by the bilayer coupling hypothesis. In the second
part shape changes and vesicle fission of vesicles composed of membranes of lipid mixtures are reported. These are explained in terms of coupling between local curvature and phase separation
Critical adsorption on curved objects
A systematic fieldtheoretic description of critical adsorption on curved
objects such as spherical or rodlike colloidal particles immersed in a fluid
near criticality is presented. The temperature dependence of the corresponding
order parameter profiles and of the excess adsorption are calculated
explicitly. Critical adsorption on elongated rods is substantially more
pronounced than on spherical particles. It turns out that, within the context
of critical phenomena in confined geometries, critical adsorption on a
microscopically thin `needle' represents a distinct universality class of its
own. Under favorable conditions the results are relevant for the flocculation
of colloidal particles.Comment: 52 pages, 10 figure
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