Cryptococcus gattii sero-mating type allelic pattern determined by multiplex PCR

AbstractMolecular methods to differentiate serotypes, mating types and molecular types of Cryptococcus neoformans and C. gattii are important tools to understand epidemiology and pathogenesis of these pathogens. In this study, a multiplex polymerase chain reaction (PCR) approach was applied to sero-mating typing of C. gattii strains. Four pairs of primers were designed to target 4 allele-specific genes located in the mating-type locus. Twenty-three C. gattii strains, presenting different mating types and serotypes, were tested to validate the method. The method was able to identify all sero-mating allelic patterns including hybrid combinations, and therefore, it represents a simple one-step PCR for sero-mating typing of C. gattii strains

Observations of IMF coherent structures and their relationship to SEP dropout events

Abstract. The solar energetic particle (SEP) events from impulsive solar flares are often characterized by short-timescale modulations affecting, at the same time, particles with different energies. Several models and simulations suggest that these modulations are observed when SEPs propagate through magnetic structures with a different connection with the flare site. However, in situ observations rarely showed clear magnetic signatures associated with these modulations. In this paper we used the Grad–Shafranov reconstruction to perform a detailed analysis of the local magnetic field topology during the SEP event of 9–10 January 1999, characterized by several SEP dropouts. An optimization procedure is used to identify, during this SEP event, the magnetic structures which better satisfy the Grad–Shafranov assumptions and to evaluate the direction of their invariant axis. We found that these two-dimensional structures, which are flux ropes or current sheets with a more complex field topology, are generally associated with the maxima in the SEP counts. This association suggests that the SEPs propagate within these structures and, since their gyration radii is much smaller than the transverse dimension of these structure, cannot escape from them

The origin of slow Alfvenic solar wind at solar minimum

Although the origins of slow solar wind are unclear, there is increasing evidence that at least some of it is released in a steady state on overexpanded coronal hole magnetic field lines. This type of slow wind has similar properties to the fast solar wind, including strongly Alfvénic fluctuations. In this study, a combination of proton, alpha particle, and electron measurements are used to investigate the kinetic properties of a single interval of slow Alfvénic wind at 0.35 au. It is shown that this slow Alfvénic interval is characterized by high alpha particle abundances, pronounced alpha–proton differential streaming, strong proton beams, and large alpha-to-proton temperature ratios. These are all features observed consistently in the fast solar wind, adding evidence that at least some Alfvénic slow solar wind also originates in coronal holes. Observed differences between speed, mass flux, and electron temperature between slow Alfvénic and fast winds are explained by differing magnetic field geometry in the lower corona

Highly Alfvenic slow solar wind at 0.3 au during a solar minimum: Helios insights for Parker Solar Probe and Solar Orbiter

Alfvénic fluctuations in solar wind are an intrinsic property of fast streams, while slow intervals typically have a very low degree of Alfvénicity, with much more variable parameters. However, sometimes a slow wind can be highly Alfvénic. Here we compare three different regimes of solar wind, in terms of Alfvénic content and spectral properties, during a minimum phase of the solar activity and at 0.3 au. We show that fast and Alfvénic slow intervals share some common characteristics. This would suggest a similar solar origin, with the latter coming from over-expanded magnetic field lines, in agreement with observations at 1 au and at the maximum of the solar cycle. Due to the Alfvénic nature of the fluctuations in both fast and Alfvénic slow winds, we observe a well-defined correlation between the flow speed and the angle between magnetic field vector and radial direction. The high level of Alfvénicity is also responsible of intermittent enhancements (i.e. spikes), in plasma speed. Moreover, only for the Alfvénic intervals do we observe a break between the inertial range and large scales, on about the timescale typical of the Alfvénic fluctuations and where the magnetic fluctuations saturate, limited by the magnitude of the local magnetic field. In agreement with this, we recover a characteristic low-frequency 1/f scaling, as expected for fluctuations that are scale-independent. This work is directly relevant for the next solar missions, Parker Solar Probe and Solar Orbiter. One of the goals of these two missions is to study the origin and evolution of slow solar wind. In particular, Parker Solar Probe will give information about the Alfvénic slow wind in the unexplored region much closer to the Sun and Solar Orbiter will allow us to connect the observed physics to the source of the plasma

First results from the Solar Orbiter Heavy Ion Sensor

Context. Aims. Solar Orbiter launched in February 2020 with the goal of revealing the connections between the Sun’s interior, atmosphere, and the heliosphere. The Solar Orbiter Heavy Ion Sensor (HIS) is a time-of-flight ion mass spectrometer dedicated to measuring heavy ions in the solar wind. Methods. We present an overview of the first measurements of heavy ion composition from HIS, reviewing the methods used to transform the spectra obtained on board into scientific data products and examining two solar wind case studies as well as the statistical properties of the heavy ion composition observed by HIS. We also carried out a comparison with prior measurements of heavy ions at L1. Results. The HIS data set provides the first mass- and charge-resolved heavy ion measurements in the inner heliosphere. Conclusions. These high temporal resolution data have the potential to transform our understanding of the connections between the solar wind and its origin at the Sun, as well as the interaction between the solar wind and the environment around planets, comets, and in the interstellar medium

The BL Lac objects OQ 530 and S5 0716+714. Simultaneous observations in the X-rays, radio, optical and TeV bands

We present the results of the BeppoSAX observations of two BL Lacs, OQ 530 and S5 0716+714, as part of a ToO program for the simultaneous observation at radio, optical, X-ray and TeV energies. Both sources are detected in the LECS and MECS, with S5 0716+714 visible also in the PDS band, up to about 60 keV. The X-ray spectra of both sources are better fitted by a double power-law model, with a steep soft X-ray component flattening at harder energies, with breaks at 0.3 and 1.5 keV, respectively. The concave shape of the spectra in both objects is consistent with soft X-rays being produced by the synchrotron and harder X-rays by the inverse Compton processes. Also the X-ray variability properties confirm this scenario, in particular for S5 0716+714 our observation shows variations by about a factor 3 over one hour below 3 keV and no variability above. Their simultaneous broad band energy spectral distributions can be successfully interpreted within the frame of a homogeneous synchrotron and inverse Compton model, including a possible contribution from an external source of seed photons with the different spectral states of S5 0716+714 being reproduced by changing the injected power. The resulting parameters are fully consistent with the two sources being intermediate objects within the "sequence" scenario proposed for blazars.Comment: 10 pages, 8 figures, accepted by A&

First Solar Orbiter observation of the Alfvénic slow wind and identification of its solar source

Context: Turbulence dominated by large amplitude nonlinear Alfvén-like fluctuations mainly propagating away from the Sun is ubiquitous in high speed solar wind streams. Recent studies have shown that also slow wind streams may show strong Alfvénic signatures, especially in the inner heliosphere. Aims: The present study focuses on the characterisation of an Alfvénic slow solar wind interval observed by Solar Orbiter on July 14-18, 2020 at a heliocentric distance of 0.64 AU. Methods: Our analysis is based on plasma moments and magnetic field measurements from the Solar Wind Analyser (SWA) and Magnetometer (MAG) instruments, respectively. We compare the behaviour of different parameters to characterise the stream in terms of the Alfvénic content and magnetic properties. We perform also a spectral analysis to highlight spectral features and waves signature using power spectral density and magnetic helicity spectrograms, respectively. Moreover, we reconstruct the Solar Orbiter magnetic connectivity to the solar sources via both a ballistic and a Potential Field Source Surface (PFSS) model. Results: The Alfvénic slow wind stream described in this paper resembles in many respects a fast wind stream. Indeed, at large scales, the time series of the speed profile shows a compression region, a main portion of the stream and a rarefaction region, characterised by different features. Moreover, before the rarefaction region, we pinpoint several structures at different scales recalling the spaghetti-like flux-tube texture of the interplanetary magnetic field. Finally, we identify the connections between Solar Orbiter in situ measurements, tracing them down to coronal streamer and pseudostreamer configurations. Conclusions. The characterisation of the Alfvénic slow wind stream observed by Solar Orbiter and the identification of its solar source are extremely important aspects to understand possible future observations of the same solar wind regime, especially as solar activity is increasing toward a maximum, where a higher incidence of this solar wind regime is expected

Magnetic reconnection as a mechanism to produce multiple protonpopulations and beams locally in the solar wind

Context. Spacecraft observations early revealed frequent multiple proton populations in the solar wind. Decades of research on their origin have focused on processes such as magnetic reconnection in the low corona and wave-particle interactions in the corona and locally in the solar wind.Aims.This study aims to highlight that multiple proton populations and beams are also produced by magnetic reconnection occurring locally in the solar wind. Methods. We use high resolution Solar Orbiter proton velocity distribution function measurements, complemented by electron and magnetic field data, to analyze the association of multiple proton populations and beams with magnetic reconnection during a period of slow Alfv\'enic solar wind on 16 July 2020. Results. At least 6 reconnecting current sheets with associated multiple proton populations and beams, including a case of magnetic reconnection at a switchback boundary, are found during this day. This represents 2% of the measured distribution functions. We discuss how this proportion may be underestimated, and how it may depend on solar wind type and distance from the Sun. Conclusions. Although suggesting a likely small contribution, but which remains to be quantitatively assessed, Solar Orbiter observations show that magnetic reconnection must be considered as one of the mechanisms that produce multiple proton populations and beams locally in the solar wind

Parker solar probe: four years of discoveries at solar cycle minimum

Launched on 12 Aug. 2018, NASA’s Parker Solar Probe had completed 13 of its scheduled 24 orbits around the Sun by Nov. 2022. The mission’s primary science goal is to determine the structure and dynamics of the Sun’s coronal magnetic field, understand how the solar corona and wind are heated and accelerated, and determine what processes accelerate energetic particles. Parker Solar Probe returned a treasure trove of science data that far exceeded quality, significance, and quantity expectations, leading to a significant number of discoveries reported in nearly 700 peer-reviewed publications. The first four years of the 7-year primary mission duration have been mostly during solar minimum conditions with few major solar events. Starting with orbit 8 (i.e., 28 Apr. 2021), Parker flew through the magnetically dominated corona, i.e., sub-Alfvénic solar wind, which is one of the mission’s primary objectives. In this paper, we present an overview of the scientific advances made mainly during the first four years of the Parker Solar Probe mission, which go well beyond the three science objectives that are: (1) Trace the flow of energy that heats and accelerates the solar corona and solar wind; (2) Determine the structure and dynamics of the plasma and magnetic fields at the sources of the solar wind; and (3) Explore mechanisms that accelerate and transport energetic particles