Wspólne cechy i różnice uczestnictwa rodzin wielopokoleniowych wiejskich w kulturze audiowizualnej

Tekst porusza zagadnienia uczestnictwa trzech generacji rodzin wielopokoleniowych wiejskich (młodej, średniej i starszej) w kulturze audiowizualnej. Zasadniczym celem jest ukazanie tego, co łączy, oraz tego, co różni poszczególne generacje rodzin wiejskich wielopokoleniowych, jeżeli chodzi o ich uczestnictwo w kulturze audiowizualnej (związane z zakresem, częstotliwością, funkcjami jakie pełnią media elektroniczne i multimedia w ich życiu). Przedstawiony został również charakter relacji zachodzących między poszczególnymi generacjami w rodzinie wielopokoleniowej wiejskiej, w kontekście ich uczestnictwa w kulturze audiowizualnej

Immunolocalization of PTHrP in the parotid glands of three rodents species: Clethrionomys glareoulus, Microtus arvalis and white Swiss mice.

The current study was inspired by the fact that since 2004 no report had appeared on the occurrence of this peptide in healthy parotid glands of humans and animals. The objective of the current study was to investigate the immunolocalization of PTHrP in the parotid gland of three male rodents: 6 common voles (Microtus arvalis, Pallas, 1779), 6 bank voles (Clethrionomys glareoulus, Schreber, 1780) and 6 white Swiss mice, as well as to find out any species differences in the distribution of this peptide in various types of cells of the parotid gland. Immunocytochemical reactions were performed using the ABC technique with specific rabbit antibodies against human PTHrP (34-53) (CALBIOCHEM), diluted 1:70 and 1:50. We observed positive PTHrP expression in the epithelial cells of the striated duct in all the three animal species. The expression was strong in white mouse and very strong in common vole and bank vole. In all the rodent species studied, the reaction for PTHrP was granular in nature and irregularly distributed in the cytoplasm, being definitely stronger at the base and weaker at the apex of the cells. The PTHrP expression was negative in the epithelium of the intercalated duct, interlobular duct, main excretory duct, as well as in the myoepithelial cells surrounding the excretory ducts or serous acini

Effect of acute exposure to cadmium on the expression of calcitonin gene-related peptide (CGRP), calcitonin (CT), somatostatin (SST) and synaptophysin (SYN) in the C cells of the rat thyroid - a preliminary study

The aim of the present study was to determine to what degree acute exposure to cadmium affects the expression of CGRP, CT, SST and SYN in the C cells of the rat thyroid. Animals from 7 experimental groups received CdCl2 iv. in doses of 3.5, 3, 2.5, 2, 1.5, 1 and 0.5 mg/kg b.w. respectively, while the control animals were given 0.9% NaCl iv. 24 hours after the iv. administration of CdCl2, a correlation was found between a single dose of cadmium and the intensity of the immunocytochemical reactions for CGRP, CT, SST and SYN in C cells of the rat thyroid when compared to the control. The weakest immunocytochemical reactions were noted in C cells of the thyroid of rats from Groups I and II, their intensity gradually increasing in Groups III, IV and V in comparison to the control. The reaction intensity in animals of Groups VI and VII resembled those of the control

A preliminary study of the submandibular gland of the rat after long-term cadmium intoxication

The aim of the study was to establish to what degree the 24-week exposure of a rat to 5 and 50 mg Cd/dm3 affects the proliferating activity of cells with PCNA and Ki-67 nuclear immunoreactivity in the submandibular gland cells. The control animals received only redistilled water to drink. The group I rats were given 5 mg Cd/dm3, while the group II animals were given 50 mg Cd/dm3. The highest concentration of cadmium was observed in group II, with a concomitant increase in the number of PCNA-positive cells. In group I, cadmium concentration was significantly less compared to group II, and there were fewer PCNA-positive cells. The reaction for Ki-67 in both experimental groups was negative

Assessment of S100 protein expression in the epididymis of juvenile and adult European bison.

In our study, we decided to compare S100 protein expression in the material obtained from the epididymes of 5- and 12-month-old calves, and adult European bison, and to detect any differences in S100 expression according to the animal age and size of the organ examined. We used the epididymes obtained from 6 adult European bison aged 6-12 years, from 6 at the age of 12 months and 6 calves aged 5 months. Immunocytochemical reactions were performed using the avidin-biotinylated-peroxidase (ABC) technique according to HSU. Specific polyclonal rabbit antiserum against bovine S100 protein (Bio Genex Laboratories) at a dilution at 1:400 was applied. We found the expression of S100 protein in endothelial cells of arteries, veins and lymphatic vessels in all the study animals. At the same time, we found no differences in the expression of S100 protein in vascular endothelial cells. Our observations seem to indicate that S100 expression in endothelial cells of European bison epididymis is not correlated with age or maturity of the organ tested. We found S100 protein in smooth muscle cells of arteries and veins in all European bison specimens examined. Interestingly in the current study, in young 5-month-old sexually immature European bison specimens we observed weaker expression of S100 protein in smooth muscle cells of small vessels as compared to the same cell type both in large vessels in these animals and in small vessels in adult specimens

Preliminary comparative immunocytochemical study of respiratory tract endocrine cells in certain rodents

Studies were performed on 3 species belonging to two families: Microtidae (7 common voles and 7 pine voles), and Muridae - 10 Wistar rats. In rodents the airways endocrine cells (ECs) are localised in the epithelium lining the larynx, trachea, bronchi, bronchioles and lung. CGRP-, synaptophysin (SY)-, calcitonin (CT)-, neuron-specific enolase (NSE)- and chromogranin A (CA)- immunoreactivity were nearly totally co-localised in ECs. In the region of the tracheo-larynx junction, CGRP- and NSE-positive cells were observed in the epithelium of the glands. It is surmised that some of the CGRP-positive ECs do not generate CT and CA, for the most part in ECs. In Microtidae ECs were more abundant than in the rat and were found even in the epithelium lining of the inside larynx in the transition region before the trachea

Angiogenesis in gliomas.

Brain gliomas are characterized by invasive growth and neovascularisation potential. Angiogenesis plays a major role in the progression of gliomas and its determination has a great prognostic value. The aim of the study was to assess the vascularisation of chosen brain gliomas and to estimate how it is correlated with tumour histological type, malignancy grade, location and size, and with age and sex of patients. Tumour vascularisation analysis was based on the determination of microvascular proliferation (MVP) and microvessel density (MVD). Microvascular proliferation was measured with immunohistochemical methods using mouse monoclonal antibodies to detect cell proliferation antigens. The following antibodies were used Ki-67 and PCNA (DAKO). Identification of vessels was performed by CD31 antibody and anti-human von Willebrand factor (DAKO). The highest microvascular proliferation and microvascular density were observed in multiform glioblastomas and the lowest in oligodendrogliomas. Significant correlation was observed between the vascularisation and malignancy grade

Histological and immunohistochemical studies on the epididymal duct in the dromedary camel (Camelus dromedarius)

This study was conducted to underscore the spatial distribution of some biologically active proteins within the epididymal duct in the dromedary camel. Paraffin-embedded sections from different regions of epididymis were stained by conventional histological techniques and by immunohistochemistry. A battery of primary antibodies against six proteins (S100, alpha smooth muscle actin [α-SMA], connexin-43 [Cx43], galactosyltransferase [GalTase], angiotensin converting enzyme [ACE], and vascular endothelial growth factor [VEGF]) were used. The epididymal epithelium consisted of five cell populations: principal, basal, apical, dark, and halo cells. The histochemical findings indicated the absence of binding sites for VEGF and Cx43. The principal cells (PCs) showed variable immunoreactivity (IR) for ACE, S100, and GalTase throughout the whole length of the duct. The apical surfaces of most PCs (at the caput) and some PCs (at the corpus) exhibited intense ACE-IR, whereas those at the cauda displayed alternating negative and strong immunostaining. Similarly, moderate S100-IR was found in cytoplasm and nuclei of all PCs at the caput, few PCs at the corpus, and several PCs alternating with negative PCs at the cauda. In contrast, only some PCs showed weak to strong GalTase-IR in different regions. Apart from negative to weak positive S100-IR, basal cells failed to show IR for all other proteins. Apical cells displayed strong IR for ACE, S100, and GalTase with some regional differences. The peritubular and vascular smooth muscle cells revealed strong α-SMA-IR in all regions. In conclusion, the spatial distribution of different proteins in camel epididymis showed similarities and differences to other mammalian species. The region-specific topographic distribution of different proteins and cell types might indicate that the caput and cauda are metabolically more active than that of the corpus