Preliminary Analysis of the Anti-biofilm Efficacy of Manuka Honey on Extended Spectrum Β-lactamase Producing Escherichia Coli Tem-3 and Klebsiella Pneumoniae Shv18, Associated with Urinary Tract Infections

open access articleUrinary Tract Infections (UTIs) are one of the most common infections in the UK and many other parts of the world. The prevalence of the Extended Spectrum β-Lactamases (ESBLs) producing UTIs, combined with their ability to form a bio film, has significantly risen and is limiting therapeutic options. This study investigated the anti-bio film activity of Manuka honey on two ESBL producing pathogens, Escherichia coli TEM-3 and Klebsiella pneumonia SHV18, commonly found in UTIs. The ESBL production was confirmed by the double disk synergy method used to confirm the ESBL production. The antibacterial activity of Manuka honey was determined using the agar well diffusion method. The Minimum Inhibitory Concentration (MIC) was established using serially diluted honey ranging from 50% to 1.56%. The effect of Manuka honey on the pathogen bio films was analysed using the Tissue Culture Plate method, with an established MIC and under 24h incubation with the honey. The results indicated that K. pneumonia SHV18 is a stronger bio film producer than E. coli TEM 3. 50% (w/v) MIC Manuka honey appears to fully prevent the plank tonic growth of both strains. A significant reduction of 81% of the E. coli TEM3 (p < 0.001) and 52% of the K. pneumonia SHV18 (p = 0.001) bio film biomass was observed. The E. coli bio films were found to be more sensitive to the 50% (w/v) honey dilution than those produced by K. pneumonia. The study indicated the anti-bio film potency of Manuka honey and its potential to become an alternative treatment for the ESBL producing pathogens associated with UTIs

Determinants of self-esteem among students from Junior High School and Socio- Therapy Center

Temat: „Uwarunkowania samooceny młodzieży gimnazjalnej i z ośrodka socjoterapii”. Praca składa się z 4 rozdziałów. Pierwszy rozdział obejmuje analizę literatury przedmiotu i zawiera następujące podrozdziały: wyjaśnienia terminologiczne, tj. ocena, samoocena, socjoterapia i ośrodek socjoterapeutyczny, charakterystykę rozwoju psycho-fizycznego młodzieży w wieku dorastania, funkcjonowanie młodzieży gimnazjalnej oraz charakterystykę placówek wychowawczych, ośrodków socjoterapeutycznych i młodzieży z tych ośrodków. Drugi rozdział to opis metodologii badań własnych. Badania przeprowadziłam w Gimnazjum nr 2 im. Powstańców Warszawy oraz w Młodzieżowym Ośrodku Socjoterapii w Skarżysku-Kamiennej. Badaniami objęłam łącznie stu respondentów, w tym 55-ciu uczniów z Gimnazjum nr 2 i 45-ciu uczniów z MOS-u oraz trzech nauczycieli z obu placówek. Trzeci rozdział zawiera analizę wyników badań własnych. W tym rozdziale zostały przedstawione wyniki badań dotyczące samooceny sfery fizycznej, psychicznej i społecznej młodzieży gimnazjalnej i z ośrodka socjoterapii. Ostatni rozdział zawiera uogólnienia i wnioski z badań.Title: „Determinants of self-esteem among students from Junior High School and Socio-Therapy Center”. The work consists of four chapters. The first chapter includes an analysis of literature and contains following sections: terminology i.e. assessment, self-esteem, sociotherapy and socio-therapy center; characteristics of psycho-physical development of youngpeople, during adolescence; junior high school student and characteristic of educational institutions, socio-therapy centers and their young members. The second chapter describes methodology and theory of conducted research. The research was carried out at Powstańców Warszawy 2nd Junior High School and Youth Socio-Therapy Center in Skarżysko-Kamienna.The study was conducted on one hundred respondents, including 55 students from Junior High School, 45 pupils from Youth Socio-Therapy Center and 3 teachers from both institutions. The third chapter provides an analysis of the results. This chapter presents outcomes of the research in the sphere of physical, psychological and social self-esteem among junior high school students and socio-therapy center members. The final chapterhighlights conclusions from the study