Genetsko poboljšanje plodnosti ovaca selekcijom po fiziološkim kvantitativnim parametrima

In this paper, fertility parameters of sheep are discussed and principles analyzed in order to obtain the optimal methods for their evaluation. Mathematical procedures for evaluation of genetic and phenotypic parameters and anticipation of genetic values, today, are mainly based on different linear and non-linear concepts, therefore it is necessary to accept the reality that there is no absolute accuracy. Special emphasis is on the possibility of use of physiological quantitative fertility indicators in sheep selection, considering that there is correlation between them and fertility traits. In this regard, the degree of response to direct and indirect selection within population is analyzed in order to established the nature of parameters necessary for evaluation of data and determine the relative degree of such response.U radu se razmatraju parametri plodnosti ovaca i analiziraju principi iznalaženja optimalnih metoda za njihovu procenu. Ističe se da su matematički postupci za procenu genetskih i fenotipskih parametara i predviđanje genetskih vrednosti danas uglavnom zasnovani na različitim linearnim i ne linearnim konceptima, pa je zbog toga potrebno prihvatiti realnost da potpuna tačnost za sada ne postoji. Poseban akcenat se stavlja na mogućnost korišćenja fizioloških kvantitativnih pokazatelja u selekciji ovaca na plodnost, s obzirom da postoji korelacija između njih i osobina plodnosti. U tom smislu, analizira se stepen odgovora na direktnu i indirektnu selekciju unutar populacije, kako bi se ustanovila priroda parametara neophodnih za ocenu podataka i utvrdili relativni stepeni takvog odgovora

Uticaj nivoa primene selekcijskih i odgajivačkih kriterijuma kao faktora održivog ovčarstva na proizvodne osobine ovaca u ekstenzivnom sistemu gajenja

For the purpose of realization of the project of development of sustainable sheep production systems, in this paper, the major influences on production parameters of sheep in conditions of Stara Planina Mountain are investigated. Using the knowledge of gene effects, with good breeding programs, it is possible to realize, with more or less success, planned annual genetic progress of traits which are of interest to the breeders. This relates first of all on gain of lambs and milk yield in sheep. As already known, traits of body development and milk production vary depending on the factors of hereditary nature, but also factors of the environment. Therefore, the level of application of selection-breeding criteria is of great practical importance, which is confirmed also in this paper, and level of significance was statistically confirmed. Also, for complete creation of the program of improvement of sheep milk and meat production it is important to be aware of the effect of the breed itself, as well as of type of birth on production parameters, which was taken into consideration during investigation, processing and presentation of data in this paper. Obtained results show that majority of observed factors had significant effect on realized production of sheep.Za razvoj održive ovčarske proizvodnje, između ostalog, neophodna je primena visokih kriterijuma selekcije i odgajivanja. Cilj ovog rada je da ukaže na praktičan značaj nivoa primene kriterijuma selekcije i odgajivanja u proizvodnji mesa i mleka ovaca u ekstenzivnom sistemu gajenja na Staroj planini. Proizvodnja mesa i mleka ovaca na Staroj planini je tradicionalna i istorijski posmatrano, uglavnom je zasnovana na gajenju pirotske pramenke, kao lokalne populacije u ekstenzivnim proizvodnim sistemima. Ova rasa kao i većina lokalnih populacija poseduje solidan genetski potencijal za prirast jagnjadi i mlečnost ovaca, ali da bi se taj potencijal ispoljio, potrebna je primena viših kriterijuma selekcije i odgajivanja, što uglavnom nije slučaj. Pored pramenke od druge polovine XX veka na području pirotskog dela Stare planine uvedena je u primenu i nova populacija-pirotska oplemenjena ovca. Ona se takođe u većini slučajeva gaji kao i pirotska pramenka, mada ove dve rase u genetskom smislu imaju veoma malo zajedničkog. Kao što je poznato, osobine telesnog razvoja i mlečnosti ovaca variraju u zavisnosti od mnogih faktora nasledne prirode ali i različitih spoljnih uticaja. Zato je nivo primene selekcijsko odgajivačkih kriterijuma, od velikog praktičnog značaja, što potvrđuju i rezultati istraživanja u ovom radu, a što je takođe i statistički potvrđeno. Pored toga za kompletno kreiranje programa unapređenja proizvodnje mesa i mleka ovaca, značajno je poznavanje uticaja same rase, kao i tipa rođenja na proizvodne parametre, o čemu se tokom istraživanja, obrade i prikazivanja dobijenih podataka vodilo posebno računa. Dobijeni rezultati istraživanja pokazuju da je većina posmatranih faktora imala značajan uticaj na ostvarenu proizvodnju.

Procena koeficijenta naslednosti za osobine porasta kod ovaca

The objective of this research paper was to estimate heritability coefficients (heritability) of growth traits in the lambs of indigenous Sjenicka Pramenka breed. The research was conducted on a sample which included 421 lambs the descendants of 15 sires and 187 sheep-dams, raised on 3 private farms in Kolubarski district, Serbia. The effect of fixed factors was studied while the values of heritability coefficients were estimated within the frame of SAS software package. A statistically highly significant (**=P lt 0.01) effect on studied traits had following parameters: sex, year of lambing, type of lambing, farm and method of applied reproductive technology. By means of REML method and using the sire model the values of heritability coefficients were determined for the following traits: average lamb birth weight (BW0), average lamb body weight at 30 days (BW30) and average lamb body weight at 90 days (BW90) being: 0.0355; 0.4642 and 0.3018, respectively.Cilj rada bio je da se procene koeficijenti naslednosti (heritabilitet) osobina porasta kod jagnjadi sjeničke pramenke. Istraživanje je sprovedeno na uzorku od 421 jagnjadi, potomaka 15 očeva i 187 ovaca-majki sa 3 privatne farme u Kolubarskom okrugu. Ispitan je uticaj fiksnih faktora i procenjene su vrednosti koeficijenata naslednosti u okviru SAS softverskog paketa. Visoko statistički značajan (**=P lt 0,01) uticaj na ispitivane osobine imali su: pol, godina jagnjenja, tip jagnjenja, farma i metod primenjene reproduktivne tehnologije. Metodom intraklasne korelacije polusrodnika po ocu utvrđene su vrednosti koeficijenta naslednosti za osobine: prosečna telesna masa jagnjadi na rođenju (BW0), prosečna telesna masa jagnjadi sa 30 dana starosti (BW30) i prosečna telesna masa jagnjadi sa 90 dana starosti (BW90) i iznosile su: 0,0355; 0,4642 i 0,3018, respektivno

Značaj bivola u proizvodnji mleka i stanje populacije u Srbiji

This research paper gives an analysis on the size of world's domestic buffalo populations, their milk production and the size of buffalo population in Serbia. Population of domestic buffalo in the world is constantly increasing so that in 2013 there were 199 783 549 individuals, out of which in India in the same year they raised 57.77% of buffalo world population, in Pakistan 18.87%, and in China 11.64%. The share of total world production of buffalo milk in total world milk production in 2012 was 12.92 % or 97 417 135 t out of which 67.76% was produced in India. In Serbia buffalo is raised in the regions of Raška (about 1000 individuals) and Kosovo. Populations of buffalo in central Serbia show a tendency of decreasing in size what was the reason to start a programme of in situ conservation 10 years ago. On the sample of buffalo population encompassed by the programme of conservation the body measures were analysed indicating that the population of buffalo is quite unequalised and that average values obtained for exterior measures are similar to the results obtained by the authors of earlier period for the population of buffalo in the area of former Yugoslavia.U radu je analizirano brojno stanje domaćih bivola u svetu, proizvodnja mleka bivola, kao i stanje populacije bivola u Srbiji. Populacija domaćih bivola u svetu stalno raste i 2013. godine bilo je 199.783.549 grla, a od tog broja u Indiji je u 2013. godini gajeno 57,77% svetske populacije bivola, u Pakistanu 18,87%, a u Kini 11,64%. Učešće ukupne svetske proizvodnje bivoljeg mleka u ukupnoj svetskoj proizvodnji mleka 2012. godine bilo je 12,92 % ili 97.417.135 t, a od toga je 67,76% proizvedeno u Indiji. U Srbiji bivoli se gaje u području Raške oblasti (oko 1000 bivola) i na području Kosova. Populacija bivola u centralnoj Srbiji ima tendenciju smanjenja veličine, zbog čega je pre 10 godina počeo da se sprovodi program konzervacije. Na uzorku populacije (37 bivolica) obuhvaćene programom in situ konzervacije utvrđene su prosečne vrednosti za telesne mere. Visina grebena bivolica u populaciji koja je merena bila je 125,97 cm, visina krsta 126,98cm, dužina trupa 142,19cm, dubina grudi 68,75 cm, širina grudi 44,48cm, a obim grudi 184,25 cm. Populacija bivolica je bila dosta neujednačena što ukazuje na odsustvo odgajivačko-selekcijskog rada kod bivola u Srbiji u prethodnim decenijama

Ispitivanje heritabiliteta i genetskih korelacija osobina mlečnosti ovaca

Considering that total variability of milk traits in sheep within single population greatly depends on factors of genetic nature, objective of this paper was to investigate the trend of genetics parameters in populations raised in Pirot region, in order to create more efficient selection program in regard to milk production. Obtained and processed results of investigation have demonstrated that investigated traits occupy central heritability values. Degree of heritability for protein and fat had higher values in relation to other observed traits. Weak and negative genetic correlation is present between fat content and other traits whereas strong and positive correlation was determined for milk quantity and fat content and protein. Statistical analysis confirmed absence of significant differences between sheep of Pirot Pramenka breed and improved Pirot population. This research has also demonstrated that in selection of sheep in regard to milk traits and production special attention should be directed to importance of genetic parameters considering that with the increase of quantity of milk not only content of fat but also of protein are reduced, which can influence the economical efficiency on farm in system of payments for milk according to its quality.U radu je izvršena analiza genetskih parametara važnijih osobina mlečnosti u populacijama pirotske pramenke i pirotske oplemenjene ovce. Dobijeni i obrađeni rezultati istraživanja su pokazali da ispitivane osobine imaju srednji nivo heritabiliteta, pri čemu su vrednosti stepena naslednosti za protein i mast veće u odnosu na ostala posmatrana svojstva, što se svakako mora imati u vidu prilikom kreiranja programa selekcije. Ustanovljena su pozitivna i negativna odstupanja u ispitivanim osobinama. Slaba i negativna korelacija prisutna je između sadržaja masti i drugih osobina dok je jaka i pozitivna povezanost utvrđena između količine mleka i količine masti. odnosno proteina. Ne postoje statistički značajne razlike između ovaca rase pirotska pramenka i pirotske oplemenjene populacije

Investigation of serum testosterone level, scrotal circumference, body mass, semen characteristics, and their correlations in developing MIS lambs

The aim of this study was to investigate changes of serum testosterone, scrotal circumference, body weight, and semen characteristics in a Meat Institute Sheep (MIS) population during their developing period (between 3 and 17 months). Correlative relationships among parameters were also tested. Body weight and scrotal circumference were measured monthly, blood samples for testosterone levels were taken bimonthly (3-17 months), and semen samples were collected by an electroejaculator between the ages of 9 and 17 months. The analysis showed that the average serum testosterone levels ranged from 1.83 to 13.28 ng/mL and significantly depended on the age of the lambs (P (lt) 0.05). The trend in the level of serum testosterone was characterized by pronounced variability throughout the study period. Changes in scrotal circumference and body weight were significantly dependent on age. Ejaculate volume, sperm motility, and the percentage of live spermatozoa increased with age, except for the percentage of abnormal spermatozoa, which decreased with age. Correlations among serum testosterone, scrotal circumference, and body weight were moderately to highly positive and highly significant. All parameters of semen quality were poorly correlated with testosterone and body weight, while scrotal circumference was in moderate correlation only with ejaculate volume and live sperm count (P (lt) 0.05)

Effects of helicobacter pylori infection on iron metabolism genes in patients with iron deficiency anaemia

This is an accepted manuscript of an article published by BMJ in Gut on 08/06/2018, available online: http://dx.doi.org/10.1136/gutjnl-2018-BSGAbstracts.282 The accepted version of the publication may differ from the final published version.Published versio

Ispitivanje genetičkih parametara osobina mlečnosti ovaca

The investigation was carried out on the sheep farm on the mountain Stara planina. The trial included two sheep genotypes: 1. Pirot pramenka sheep and 2. Improved Pirot sheep. In order to estimate necessary parameters the least squares method was used. The obtained results show that heritability of milk yield was 0.35 and 0.33, fat percent amounted to 0.49 and 0.44, while protein percent was 0.50 and 0.49 (in the 1st and 2nd genotype, respectively). Results on the genetic correlation between important parameters of milk showed that there were both, positive and negative correlations. Coefficient estimations of genetic correlations of certain traits varied from very weak to strong. Correlations between fat yield and milk yield were 0.87 and 0.88, but the coefficient between protein percent and fat yield was -0.01 and -0.04, in the 1st i.e. 2nd genotype, respectively. There are no significant differences between observed genotypes.ispitivanje genetičkih parametara (heritabilnosti i genetičkih korelacija) osobina mlečnosti ovaca je bilo cilj rada. Ispitivanja su obavljena na jednoj farmi ovaca u regionu Stare planine, kod dva genotipa (pirotska pramenka i pirotska oplemenjena ovca). Heritabilnost varira u granicama srednjih vrednosti sa maksimumom kod sadržaja proteina. Ustanovljena je kako, pozitivna, tako i negativna genetička korelacija posmatranih osobina mlečnosti ovaca. Vrednosti koeficijenata se kreću od slabih do jakih, a nisu utvrđene značajne razlike između genotipova

Validation of epigenetic markers to identify colitis associated cancer: Results of module 1 of the ENDCAP-C study

BACKGROUND:Chronic inflammation caused by ulcerative colitis (UC) causes a pro-neoplastic drive in the inflamed colon, leading to a markedly greater risk of invasive malignancy compared to the general population. Despite surveillance protocols, 50% of cases proceed to cancer before neoplasia is detected. The Enhanced Neoplasia Detection and Cancer Prevention in Chronic Colitis (ENDCaP-C) trial is an observational multi-centre test accuracy study to ascertain the role of molecular markers in improving the detection of dysplasia. We aimed to validate previously identified biomarkers of neoplasia in a retrospective cohort and create predictive models for later validation in a prospective cohort. METHODS:A retrospective analysis using bisulphite pyrosequencing of an 11 marker panel (SFRP1, SFRP2, SRP4, SRP5, WIF1, TUBB6, SOX7, APC1A, APC2, MINT1, RUNX3) in samples from 35 patients with cancer, 78 with dysplasia and 343 without neoplasia undergoing surveillance for UC associated neoplasia across 6 medical centres. Predictive models for UC associated cancer/dysplasia were created in the setting of neoplastic and non-neoplastic mucosa. FINDINGS:For neoplastic mucosa a five marker panel (SFRP2, SFRP4, WIF1, APC1A, APC2) was accurate in detecting pre-cancerous and invasive neoplasia (AUC = 0.83; 95% CI: 0.79, 0.88), and dysplasia (AUC = 0.88; (0.84, 0.91). For non-neoplastic mucosa a four marker panel (APC1A, SFRP4, SFRP5, SOX7) had modest accuracy (AUC = 0.68; 95% CI: 0.62,0.73) in predicting associated bowel neoplasia through the methylation signature of distant non-neoplastic colonic mucosa. INTERPRETATION:This multiplex methylation marker panel is accurate in the detection of ulcerative colitis associated dysplasia and neoplasia and is currently being validated in a prospective clinical trial. FUNDING:The ENDCAP-C study was funded by the National Institute for Health Research Efficacy and Mechanism Evaluation (EME) Programme (11/100/29).The ENDCAP-C study was funded by the Efficacy and Mechanism Evaluation (EME) Programme (11/100/29), an MRC and NIHR partnership. Assay development supported by Birmingham Experimental Medicine Cancer Centre. AB acknowledges funding from the Wellcome Trust (102732/Z/13/Z), Cancer Research UK (C31641/A23923) and the Medical Research Council (MR/M016587/1). JJD and DGM received support as an NIHR Senior Investigator and JJD from the NIHR Birmingham Biomedical Research Centre.Published versio