Social Capital in Boston: Findings From the Social Capital Community Benchmark Survey

Highlights survey findings on the ways in which Bostonians connect and how these social networks benefit the entire community. Ranks community involvement in Boston, including politics, activism in the arts, and tolerance, among forty sites nationwide

Development of stationary phase gradients and multi-functional stationary phases in capillary formats

The following thesis provides an extensive study into the fabrication of polymer monolithic columns in capillary housing, their modification and subsequent characterisation. The fabricated columns, in all instances, were profiles using scanning capacitively coupled contactless conductivity detection (sC4D), a noninvasive method of characterisation, which can provide information on the density of the monolithic column, and the distribution of charged functional groups within the column. Using thermally initiated co-monomer polymerisation, monolithic columns were fabricated within fused silica capillaries. The resulting distribution of charged functional groups was monitored using sC4D. Thermally initiated gradient monolithic columns produced linear gradients, however, the capacity produced was sufficiently low, as determined by the retention factor, k ( k < 1). Alternatively photo-initiated modification of polymer monoliths was investigated using commercially available optical filters, with a gradient of optical density. However, due to the optical density of the filter, the gradient did not span the desired length of the column. In the place of a linear gradient, a photo-grafted stepped gradient was produced and characterised using sC4D. The column was successfully applied to the separation of two metal cations. However, the resulting stepped gradient was not ideal for separations. To resolve this problem, an optical filter was developed (in-house). The filter was comprised of commercially available COC films which demonstrated attenuation of UV energy with increasing thickness, resulting in a linear photo-grafted gradient. The column was profiled using sC4D, thus providing a novel, indirect characterisation of the optical filter (complementary to UV/VIS analysis). Using photo-grafting techniques, a gold nano-particle agglomerated segment of a monolithic column was created. This provided a stationary phase with dualfunctionality, which was applied to the on-line extraction of proteins, followed by reversed-phase chromatography. The columns were characterised using techniques such as field emission SEM and sC4D. This provided a novel application of sC4D

Improving on the Contingent Fee

Two basic fees--contingent and hourly--dominate the variety of fees that lawyers charge clients for pursuing damage claims. Each of these two types has its advantages; each is plagued with substantial disadvantages. This Article proposes a new type of fee, one that preserves the respective advantages of the two present fees while minimizing their distinct disadvantages. In essence, the proposed fee calls for the payment, on a contingent basis, of an amount computed by adding one component tied to hours worked and another component linked to amount recovered. The preferability and feasibility of this proposed fee argue for the abolishment, or at least for the severe restriction, of the contingent fee as it is now known; the hourly fee should continue as a client\u27s option

Improving on the Contingent Fee

Two basic fees--contingent and hourly--dominate the variety of fees that lawyers charge clients for pursuing damage claims. Each of these two types has its advantages; each is plagued with substantial disadvantages. This Article proposes a new type of fee, one that preserves the respective advantages of the two present fees while minimizing their distinct disadvantages. In essence, the proposed fee calls for the payment, on a contingent basis, of an amount computed by adding one component tied to hours worked and another component linked to amount recovered. The preferability and feasibility of this proposed fee argue for the abolishment, or at least for the severe restriction, of the contingent fee as it is now known; the hourly fee should continue as a client\u27s option

Individual monitoring for internal exposure in Europe and the integration of dosimetric data

The European Radiation Dosimetry Group, EURADOS, established a working group consisting of experts whose aim is to assist in the process of harmonisation of individual monitoring as part of the protection of occupationally exposed workers. A catalogue of facilities and internal dosimetric techniques related to individual monitoring in Europe has been completed as a result of this EURADOS study. A questionnaire was sent in 2002 to services requesting information on various topics including type of exposures, techniques used for direct and indirect measurements including calibration and sensitivity data and the methods employed for the assessment of internal doses. Information relating to Quality Control procedures for direct and indirect measurements, Quality Assurance Programmes in the facilities and legal requirements for ‘approved dosimetric services' were also considered. A total of 71 completed questionnaires were returned by internal dosimetry facilities in 26 countries. This results in an overview of the actual status of the processes used in internal exposure estimation in Europe. In many ways harmonisation is a reality in internal dose assessments, especially when taking into account the measurements of the activity retained or excreted from the body. However, a future study detailing the estimation of minimum detectable activity in the laboratories is highly recommended. Points to focus on in future harmonisation activities are as follows: the process of calculation of doses from measured activity, establishment of guidelines, similar dosimetric tools and application of the same ICRP recommendations. This would lead to a better and more harmonised approach to the estimation of internal exposures in all European facilitie

Thermoluminescent detectors applied in individual monitoring of radiation workers in Europe—a review based on the EURADOS questionnaire

Among the activities of EURADOS Working Group 2 formed by experts from several European countries is the harmonisation of individual monitoring as part of radiation protection of occupationally exposed persons. Here, we provide information about thermoluminescent detectors (TLDs) applied by the European dosimetric services and the dosimetric characteristics of dosemeters in which these detectors are applied. Among 91 services from 29 countries which responded to the EURADOS questionnaire, 61 apply dosemeters with TLDs for the determination of personal dose equivalent Hp(10) for photons and beta radiation, and 16 services use TLDs for neutron albedo dosemeters. Those most frequently used are standard lithium fluoride TLDs (mainly TLD-100, TLD-700, Polish MTS-N and MTS-7, Russian DTG-4), high-sensitive lithium fluoride (GR-200, MCP-N) and lithium borate TLDs. Some services use calcium sulphate and calcium fluoride detectors. For neutron dosimetry, most services apply pairs of LiF:Mg,Ti TLDs with 6Li and 7Li. The characteristics (energy response) of individual dosemeters are mainly related to the energy response of the detectors and filters applied. The construction of filters in dosemeters applied for measurements of Hp(10) and their energy response are also reviewe

Workplace monitoring for exposures to radon and to other natural sources in Europe: integration of monitoring for internal and external exposures

Part of the action of the EURADOS working group (European Radiation Dosimetry Group) on ‘Harmonisation of Individual Monitoring in Europe' was to investigate how the results from personal dosemeters for external radiation, from monitoring for internal exposure and from workplace monitoring, can be combined into a complete and consistent system of individual monitoring. To facilitate this work, the ‘EURADOS questionnaire Q3' relating to radon and other natural sources of radiation in the workplace was distributed to relevant institutes across Europe. A total of 24 countries replied to the questionnaire. This study offers an important overview on actual regulations, national standards and reference levels for protection of employees from radon and other natural sources in different workplace scenarios. Information was also collected on individual monitoring and area monitoring to determine individual doses in workplaces with elevated levels of natural radiation. The article discusses in detail the results obtained showing by country the reference level in workplaces for radon gas and other natural sources. In both instances, exposures in mines, other underground workplaces, industry workplaces/waterworks, offices, schools and day-care homes were considered. The resultant data clearly indicate that there is a need for harmonisation among countries, not least in the areas of regulation and use of reference levels in the workplac

Immobilized-Enzyme Reactors Integrated into Analytical Platforms: Recent Advances and Challenges

Immobilized-enzyme reactors (IMERs) are flow-through devices containing enzymes that are physically confined or localized with retention of their catalytic activities. IMERs can be used repeatedly and continuously and have been applied for (bio)polymer degradation, proteomics, biomarker discovery, inhibitor screening, and detection. Online integration of IMERs with analytical instrumentation, such as high-performance liquid chromatography (HPLC) systems, reduces the time needed for multi-step workflows, reduces the need for sample handling, and enables automation. However, online integration can also be challenging, as reaching its full potential requires complex instrumental setups and experienced users. This review aims to provide an assessment of recent advances and challenges in online IMER-based (analytical) LC platforms, covering publications from 2014-2021. A critical discussion of challenges often encountered in IMER fabrication, sample preparation, integration into the analytical workflow, long-term usage, and of potential ways to overcome these is provided. Finally, the obstacles preventing the proliferation of IMERs as efficient tools for high-throughput pharmacological, industrial, and biological studies are discussed

Confinement of Monolithic Stationary Phases in Targeted Regions of 3D-Printed Titanium Devices Using Thermal Polymerization

Abstract In this study, we have prepared thermally initiated polymeric monolithic stationary phases within discrete regions of 3D-printed titanium devices. The devices were created with controllable hot and cold regions. The monolithic stationary phases were first locally created in capillaries inserted into the channels of the titanium devices. The homogeneity of the monolith structure and the interface length were studied by scanning a capacitively coupled conductivity contactless detector (C4D) along the length of the capillary. Homogeneous monolithic structures could be obtained within a titanium device equipped with a hot and cold jacket connected to two water baths. The confinement method was optimized in capillaries. The sharpest interfaces (between monolith and empty channel) were obtained with the hot region maintained at 70 °C and the cold region at 4 or 10 °C, with the latter temperature yielding better repeatability. The optimized conditions were used to create monoliths bound directly to the walls of the titanium channels. The fabricated monoliths were successfully used to separate a mixture of four intact proteins using reversed-phase liquid chromatography. Further chromatographic characterization showed a permeability (Kf) of ∼4 × 10–15 m2 and a total porosity of 60%. Since their introduction in the chromatographic world, porous polymer monoliths have proven to be powerful separation media. These chromatographic supports have been widely applied for applications, such as microscale liquid chromatography (LC) of peptides and proteins, but have also been used in capillary electrochromatography (CEC),(1) gas chromatography (GC),(2) sample preparation,(3) and catalysis.(4) The ease of preparation of monoliths, diverse chemistry options, and high permeabilities have made them popular materials for analytical devices, such as microfluidic chips for LC. In the past decade, miniaturization has been realized by developing lab-on-a-chip solutions, where several analytical processes can be integrated within a few square centimeters. In such systems, due to the small channels and articulated geometries, the particle-packing procedure has proven to be challenging.(5) In contrast, monolithic beds are usually created in situ by free-radical polymerization of monomers in the presence of porogens and they are well-suited for chip-based separations. The proliferation of microfluidic devices has spurred new interest in polymer monoliths for applications such as enzymatic reactors(6,7) and microfluidic mixers.(8) This development has been boosted by the advent of additive manufacturing (or 3D-printing), which allows for rapid prototyping of complex structures, converting computer-aided-design (CAD) models into physical objects. Unfortunately, the use of 3D-printed analytical devices for chromatographic analysis is limited by the solvent compatibility of some materials (e.g., acrylate-based polymers) and in some cases by their transparency at the desired wavelength (e.g., UV or IR wavelengths). Several successful steps have been taken to locally photopolymerize monolithic stationary phases in discrete regions of microfluidic devices.(9−12) Heat is an alternative way to transfer energy to the monomer precursors for initiating the polymerization. However, accurate control of temperature in small confined spaces is more difficult to achieve, and so far only few steps have been taken in this direction.(13) In this work, two methods are explored to achieve confined thermal polymerization. The first approach involves direct contact (DC) between Peltier elements and the surface of a titanium device. In the second approach, recirculating jackets are used for localized heating and cooling (heating/cooling jackets, HCJ). The latter approach resembles a recirculation-based freeze–thaw valve.(14) In both approaches, defined hot (HR) and cold (CR) regions are created. We aim to fabricate poly(styrene-co-divinylbenzene) (PS-DVB) monolithic stationary phases within a 3D-printed titanium microfluidic device through polymerization at 70 °C, and to separate intact proteins using this device