Le germoplasme agrumes de San Giuliano : une base pour la production de plants CAC et certifiés

National audienc

Karyological analysis and DNA barcoding of pompia citron: A first step toward the identification of its relatives

open7Pompia is a citrus fruit endemic of Sardinia, Italy, with an essential oil profile showing outstanding anti-inflammatory and anti-microbic properties. Despite its remarkable pharmaceutical potential, little taxonomic and genetic information is available for this species. We applied flow cytometry and classical cytogenetic techniques to assess the DNA content and to reconstruct the karyotype of several Pompia accessions. Molecular data from plastid DNA barcoding and nuclear DNA sequencing were used to study the genetic distance between Pompia and other citrus species. Flow cytometric estimates of DNA content and somatic chromosome counts suggest that Pompia is a regular diploid Citrus species. DNA polymorphisms of nuclear and chloroplast markers allowed us to investigate the genetic relationships between Pompia accessions and other Citrus species. Based on DNA polymorphism data we propose that Pompia is a very recent interspecific hybrid generated by a cross between C. aurantium (as seed bearer) and C. medica (as pollen donor). Our findings pave the way for further and more specific investigations of local Pompia germplasm resources that may help the preservation and valorisation of this valuable citrus fruit tree.openViglietti G.; Galla G.; Porceddu A.; Barcaccia G.; Curk F.; Luro F.; Scarpa G.M.Viglietti, G.; Galla, G.; Porceddu, A.; Barcaccia, G.; Curk, F.; Luro, F.; Scarpa, G. M

Volatile and Flavonoid Composition of the Peel of Citrus medica L. var. Corsican Fruit for Quality Assessment of Its Liqueur

U radu je ispitan sastav hlapljivih tvari i flavonoida u kori citrona (Citrus medica L. var. Corsican) uzgojenog na Korzici, različitog stupnja zrelosti izračunatog pomoću sume aktivnih temperatura. Razlike u stupnju razvoja plodova kvantitativno su određene plinskom kromatografijom, plinskom kromatografijom u kombinaciji s masenom spektrometrijom, te tekućinskom kromatografijom praćenom tandemskom masenom spektrometrijom. Identificirano je 30 hlapljivih komponenata eteričnog ulja dobivenog iz kore citrona, a najzastupljeniji su bili limonen i γ-terpinen. Komercijalno proizvedeni liker od citrona također je imao veliki udjel monoterpenskih ugljikovodika, među kojima su prevladavala ta ista dva spoja. Glavni su flavonoidi u citronu i likeru bili rutin i neohesperidin. Ovaj se postupak karakterizacije spojeva može primijeniti za procjenu kakvoće prehrambenih proizvoda dobivenih iz ploda citrona (C. medica var. Corsican).The volatile and flavonoid compositions of the peel of Citrus medica L. var. Corsican fruits cultivated in Corsica were studied according to the maturity of the citron fruits measured using growing degree-days. Quantitative variation with the stage of development of the fruit was observed using gas chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry/mass spectrometry. Thirty volatile compounds were identifi ed in the peel essential oil. Limonene and γ-terpinene were the major compounds. The volatile compositions of commercial citron liqueurs were also characterized by high amounts of monoterpene hydrocarbons with the same two major components. The main fl avonoid components of citron fruits and derived liqueurs were rutin and neohesperidin. This chemical characterization can be used for quality assessment of food products from C. medica var. Corsican