7 research outputs found

    Crystal structure of (2-((4-bromo-2,6-dichlorophenyl)amino)phenyl) (morpholino)methanone, C17H15BrCl2N2O2

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    C17H15BrCl2N2O2, monoclinic, P21/c (no. 14), a = 20.333(7) Å, b = 11.226(5) Å, c = 7.843(3) Å, β = 99.651(8)°, V = 1764.8(12) Å3, Z = 4, Rgt(F) = 0.0464, wRref(F2) = 0.1601, T = 173 K

    Optimal cultivation of simultaneous ammonium and phosphorus removal aerobic granular sludge in A/O/A sequencing batch reactor and the assessment of functional organisms

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    <div><p>In this study, sequencing batch reactor (SBR) with an anaerobic/aerobic/anoxic operating mode was used to culture granular sludge. Optimal adjustment of cycle duration was achieved by the direction of pH, oxidation reduction potential and dissolved oxygen parameters. The results showed that the treating efficiency was significantly improved as the cycle was shortened from 450 to 360 min and further to 200 min. Nitrogen and phosphorus removal were nearly quantitative after 50 days operation and maintained stable to the end of the study period. The typical cycle tests revealed that simultaneous denitrification and phosphorus removal occurred when aerobic granules were gradually formed. The nitrite effect tests showed that less than 4.8 mg N/L of the nitrite could enhance superficial specific aerobic phosphate uptake rate (SAPUR) under aerobic condition, indicating that the traditional method to evaluate the capability of total phosphate-accumulating organisms (PAOs) was inaccurate. Additionally, a high level of nitrite was detrimental to PAOs. A novel method was developed to determine the activity of each kind of PAOs and other denitrifying organisms. The results showed that (1) nitrate, besides nitrite, could also enhance SAPUR and (2) aerobic granular sludge could perform denitrification even when phosphate was not supplied under anoxic condition, suggesting that other denitrifying organisms besides denitrifying phosphate-accumulating organisms also contributed to denitrification.</p></div

    Benthic Microbial Communities and Environmental Parameters of Estuary and Hypoxic Zone in the Bohai Sea, China

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    The sediment microbial community plays a key role in the cycling of organic matter in marine ecosystems. The characteristics of the sediment microbial community are significantly related to changes in the environment. In this study, we analyzed the diversity and distribution of microbial communities in sediments from different geographical regions (the Liao River estuary, Yellow River estuary, hypoxic zone, and offshore zone) of the Bohai Sea using high-throughput sequencing. The results showed that the community richness and diversity (especially the unique diversity) of the Liao River estuary were larger than those of the Yellow River estuary, hypoxic zone, and offshore zone. The phylum Proteobacteria dominated in the Liao River estuary (46.26%), hypoxic zone (76.19%), and offshore zone (69.79%), while the dominant phylum in the Yellow River estuary was the Bacteroidetes phylum. The genus Gillisia was the dominant genus in both the Liao River estuary and Yellow River estuary. The offshore zone and hypoxic zone shared the same dominant Photobacterium genus. The results of Pearson correlation and redundancy analysis showed that environmental parameters such as nitrite, silicate, nitrate, phosphate, ammonia, dissolved oxygen, pH, and salinity interfered significantly with the structure and diversity of the microbial community. The results of this study will provide support for future research on the interaction mechanism of pollutants and microorganisms in the estuaries and a typical hypoxic zone of the Bohai Sea

    Response of the Intertidal Microbial Community Structure and Metabolic Profiles to Zinc Oxide Nanoparticle Exposure

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    The toxicity of nanomaterials to microorganisms is related to their dose and environmental factors. The aim of this study was to investigate the shifts in the microbial community structure and metabolic profiles and to evaluate the environmental factors in a laboratory scale intertidal wetland system exposed to zinc oxide nanoparticles (ZnO NPs). Microbial assemblages were determined using 16S rRNA high-throughput sequencing. Community-level physiological profiles were determined using Biolog-ECO technology. Results showed Proteobacteria was the predominant (42.6%&ndash;55.8%) phylum across all the sediments, followed by Bacteroidetes (18.9%&ndash;29.0%). The genera Azoarcus, Maribacter, and Thauera were most frequently detected. At the studied concentrations (40 mg&middot;L&minus;1, 80 mg&middot;L&minus;1, 120 mg&middot;L&minus;1), ZnO NPs had obvious impacts on the activity of Proteobacteria. Adverse effects were particularly evident in sulfur and nitrogen cycling bacteria such as Sulfitobacter, unidentified_Nitrospiraceae, Thauera, and Azoarcus. The alpha diversity index of microbial community did not reflect stronger biological toxicity in the groups with high NP concentrations (80 mg&middot;L&minus;1, 120 mg&middot;L&minus;1) than the group with low NP concentration (40 mg&middot;L&minus;1). The average well color development (AWCD) values of periodically submersed groups were higher than those of long-term submersed groups. The group with NP concentration (40 mg&middot;L&minus;1) had the lowest AWCD value; those of the groups with high NP concentrations (80 mg&middot;L&minus;1, 120 mg&middot;L&minus;1) were slightly lower than that of the control group. The beta diversity showed that tidal activity shaped the similar microbial community among the periodically submerged groups, as well as the long-term submerged groups. The groups with high DO concentrations had higher diversity of the microbial community, better metabolic ability, and stronger resistance to ZnO NPs than the groups with a low DO concentration
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