بررسی نتایج حاصل از اجرای الگوی ارتقاء کیفیت مدیریت مواد زائد در بیمارستان شهید فقیهی شیراز طی سال 1384

مقدمه: رشد جمعیت و توسعه شهر نشینی افزایش بی رویه انواع ضایعات در تمامی کشورها را به همراه داشته است . امروزه دفع این پسمانده ها بویژه در بیمارستانها به عنوان یکی از مهمترین چالشهای زیست – محیطی در کشورهای توسعه یافته و در حال توسعه درآمده است. در این میان تخلیه پسمانده های خطرناک صنعتی و بیمارستانی ، بدون رعایت ملاحظات زیست محیطی و فنی به عنوان یکی از پیچیده ترین و پرهزینه ترین مشکلات مسئولان در هر کشوری محسوب می شود. لذا این پژوهش با هدف کاهش میزان تولید مواد زائد عفونی بیمارستان با استفاده از الگوی ارتقاء کیفیت مدیریت مواد زائد انجام گردید. روش بررسی: در این پژوهش مداخله ای که در یکی از بیمارستانهای آموزشی شیراز انجام گرفت تلاش شد تا با اجرای یک الگوی ارتقاء کیفیت مدیریت مواد زائد از میزان تولید مواد زائد عفونی بیمارستان کاسته شود. در این پژوهش طی دو مرحله قبل و بعد از اجرای الگو زباله های بیمارستانی وزن (بر حسب کیلوگرم) و مقایسه شدند. برای تحلیل داده ها از شاخص های آمار توصیفی استفاده شد. یافته ها: نتایج نشان داد که قبل از اجرای الگو میانگین روزانه تولید زباله در بیمارستان برای زباله های عفونی 813 کیلوگرم و برای زباله های عادی83 کیلوگرم بوده، همچنین میانگین روزانه تولید زباله عفونی در بخشهای بستری 43/3 کیلوگرم به ازاء تخت روز اشغالی بوده است . میزان تولید زباله عفونی در بیمارستان 7/90% کل زباله های بیمارستان را تشکیل می داد که بعد از اجرای الگوی ارتقاء کیفیت مدیریت مواد زائد این میزان به 6/57% کاهش یافت. نتیجه گیری: استفاده از مدل ارتقاء کیفیت مدیریت مواد زائد میزان تولید زباله عفونی را تا 1/33% کاهش داده است. بنابراین بیمارستانها می توانند با استفاده از مدل ارتقاء کیفیت مواد زائد، زباله های عفونی خود را کاهش دهند

The novel protein C3orf43 accelerates hepatocyte proliferation

Abstract Background Our previous study found that single-pass membrane protein with coiled-coil domains 1 (C3orf43; XM_006248472.3) was significantly upregulated in the proliferative phase during liver regeneration. This indicates that C3orf43 plays a vital role in liver cell proliferation. However, its physiological functions remains unclear. Methods The expressions of C3orf43 in BRL-3A cells transfected with C3orf43-siRNA (C3-siRNA) or overexpressing the vector plasmid pCDH-C3orf43 (pCDH-C3) were measured via RT-qPCR and western blot. Cell growth and proliferation were determined using MTT and flow cytometry. Cell proliferation-related gene expression was measured using RT-qPCR and western blot. Results It was found that upregulation of C3orf43 by pCDH-C3 promoted hepatocyte proliferation, and inhibition of C3orf43 by C3-siRNA led to the reduction of cell proliferation. The results of qRT-PCR and western blot assay showed that the C3-siRNA group downregulated the expression of cell proliferation-related genes like JUN, MYC, CCND1 and CCNA2, and the pCDH-C3 group upregulated the expression of those genes. Conclusion These findings reveal that C3orf43 may contribute to hepatocyte proliferation and may have the potential to promote liver repair and regeneration

Rat hepatocytes weighted gene co-expression network analysis identifies specific modules and hub genes related to liver regeneration after partial hepatectomy.

The recovery of liver mass is mainly mediated by proliferation of hepatocytes after 2/3 partial hepatectomy (PH) in rats. Studying the gene expression profiles of hepatocytes after 2/3 PH will be helpful to investigate the molecular mechanisms of liver regeneration (LR). We report here the first application of weighted gene co-expression network analysis (WGCNA) to analyze the biological implications of gene expression changes associated with LR. WGCNA identifies 12 specific gene modules and some hub genes from hepatocytes genome-scale microarray data in rat LR. The results suggest that upregulated MCM5 may promote hepatocytes proliferation during LR; BCL3 may play an important role by activating or inhibiting NF-kB pathway; MAPK9 may play a permissible role in DNA replication by p38 MAPK inactivation in hepatocytes proliferation stage. Thus, WGCNA can provide novel insight into understanding the molecular mechanisms of LR

Metabolome analysis reveals flavonoid changes during the leaf color transition in Populus × euramericana ‘Zhonghuahongye’

IntroductionTo investigate the mechanism of leaf color change at different stages in Populus × euramericana ‘Zhonghuahongye’ (‘Zhonghong’ poplar).MethodsLeaf color phenotypes were determined and a metabolomic analysis was performed on leaves at three stages (R1, R2 and R3).ResultsThe a*, C* and chromatic light values of the leaves decreased by 108.91%, 52.08% and 113.34%, while the brightness L values and chromatic b* values gradually increased by 36.01% and 13.94%, respectively. In the differential metabolite assay, 81 differentially expressed metabolites were detected in the R1 vs. R3 comparison, 45 were detected in the R1 vs. R2 comparison, and 75 were detected in the R2 vs. R3 comparison. Ten metabolites showed significant differences in all comparisons, which were mostly flavonoid metabolites. The metabolites that were upregulated in the three periods were cyanidin 3,5-O-diglucoside, delphinidin, and gallocatechin, with flavonoid metabolites accounting for the largest proportion and malvidin 3- O-galactoside as the primary downregulated metabolite. The color shift of red leaves from a bright purplish red to a brownish green was associated with the downregulation of malvidin 3-O-glucoside, cyanidin, naringenin, and dihydromyricetin.DiscussionHere, we analyzed the expression of flavonoid metabolites in the leaves of ‘Zhonghong’ poplar at three stages and identified key metabolites closely related to leaf color change, providing an important genetic basis for the genetic improvement of this cultivar

Additional file 1: of Comprehensive CircRNA expression profile and selection of key CircRNAs during priming phase of rat liver regeneration

The results of annotating circRNA host linear transcripts and analysing differentially expressed circRNAs at 2 h and 6 h after PH compared with CG. (XLSX 71 kb

Numbers of the regulated and the co-expression genes that related to the LncRNAs.

<p>Numbers of the regulated and the co-expression genes that related to the LncRNAs.</p

Expression Profile and Function Analysis of LncRNAs during Priming Phase of Rat Liver Regeneration

<div><p>Emerging evidences have revealed that long non-coding RNAs (lncRNAs) functioned in a wide range of physiological and pathophysiological processes including rat liver regeneration, and could regulate gene expression in the transcriptional and post-transcriptional levels. However, the underlying mechanism for lncRNAs participation in liver regeneration is largely unknown. To define the mechanisms how the lncRNAs regulate LR, we performed bio-chip technology, high-throughput sequencing and RT-PCR to detect the expression of lncRNAs at 0, 2 and 6 h during LR after 2/3 hepatectomy (PH). The results indicated that 28 lncRNAs were involved in LR. Bioinformatics analysis predicated 465 co-expression target genes including 10 regulatory genes were related to these 28 lncRNAs. Ingenuity Pathway Analysis (IPA) was employed to analyze the signaling pathways and physiological activities that regulated by these genes, and the results suggested that these genes were potentially related to ILK, SAPK/JNK and ERK/MAPK signaling pathways, and possibly regulate many important physiological activities in LR in terms of cell proliferation, cell differentiation, cell survival, apoptosis and necrosis.</p></div

The heat maps of signaling pathways and biological functions in which significantly expressed genes were involved in rat liver regeneration.

<p>(A) The heat map of the target genes-related signaling pathways. (B) The heat maps of target genes-related biological functional.</p

Numbers of the regulated and the co-expression genes that related to the LncRNAs.

<p>Numbers of the regulated and the co-expression genes that related to the LncRNAs.</p