Yield response of fennel (Foeniculum vulgare Mill.) to irrigation with saline water

Salinity causes yield reductions if threshold levels are exceeded, while it can cause the deterioration of some qualitative parameters and/or the improvement of others. Two years (2000-2001, 2006-2007) research was carried out to evaluate the effect of water quality on yield and quality of fennel, a vegetable very common in Apulia region. We compared two types of soil and two sodium absorption ratio of irrigation water obtained by dissolving in distilled water NaCl + CaCl2 (1:1) or only NaCl, and four electrical conductivity of water of 0.7 (control), 4, 8 and 12 dS m(-1). The soil type does not show any noticeable effect on the tested parameters. The salt type influenced the plant development and the total soluble solids contained in the bulbs. The plant height, the leaf and the bulb weight were, respectively, 6, 11 and 21% higher in NaCl + CaCl2 treatment as compared to NaCl one. The plant height, the leaf and the bulb weight dropped, respectively, by 33, 49 and 71%, shifting from 0.7 to 12 dS m(-1) salinity treatments. The dry matter content and total soluble solids increased with rising salinity. In fact, shifting from the control to the more saline treatment, the leaves and the bulbs dry matter increased, respectively, by 15 and 13%, and total soluble solids increased by 17%. Moreover, the increase in salinity favoured the production of flattened bulbs. The application of the Maas and Hoffman model to salinity response confirms the ranking of this species among moderately salt sensitive species, even though the critical threshold was the same for NaCl and NaCl + CaCl2 treatments (1.26 dS m(-1)), while the slope was higher in NaCl treatment (14.24% m dS(-1)) than NaCl + CaCl2 (10.39% m dS(-1))

A Deep Learning Approach to Ancient Egyptian Hieroglyphs Classification

Nowadays, advances in Artificial Intelligence (AI), especially in machine and deep learning, present new opportunities to build tools that support the work of specialists in areas apparently far from the information technology field. One example of such areas is that of ancient Egyptian hieroglyphic writing. In this study, we explore the ability of different convolutional neural networks (CNNs) to classify pictures of ancient Egyptian hieroglyphs coming from two different datasets of images. Three well-known CNN architectures (ResNet-50, Inception-v3 and Xception) were taken into consideration and trained on the available images. The paradigm of transfer learning was tested as well. In addition, modifying the architecture of one of the previous networks, we developed a specifically dedicated CNN, named Glyphnet, tailoring its complexity to our classification task. Performance comparison tests were carried out and Glyphnet showed the best performances with respect to the other CNNs. In conclusion, this work shows how the ancient Egyptian hieroglyphs identification task can be supported by the deep learning paradigm, laying the foundation for information tools supporting automatic documents recognition, classification and, most importantly, the language translation task

Ultrasound-Responsive Nrf2-Targeting siRNA-Loaded Nanobubbles for Enhancing the Treatment of Melanoma

The siRNA-mediated inhibition of nuclear factor E2-related factor 2 (Nrf2) can be an attractive approach to overcome chemoresistance in various malignant tumors, including melanoma. This work aims at designing a new type of chitosan-shelled nanobubble for the delivery of siRNA against Nrf2 in combination with an ultrasound. A new preparation method based on a water–oil–water (W/O/W) double-emulsion was purposely developed for siRNA encapsulation in aqueous droplets within a nanobubble core. Stable, very small NB formulations were obtained, with sizes of about 100 nm and a positive surface charge. siRNA was efficiently loaded in NBs, reaching an encapsulation efficiency of about 90%. siNrf2-NBs downregulated the target gene in M14 cells, sensitizing the resistant melanoma cells to the cisplatin treatment. The combination with US favored NB cell uptake and transfection efficiency. Based on the results, nanobubbles have shown to be a promising US responsive tool for siRNA delivery, able to overcome chemoresistance in melanoma cancer cells

Assessment of multispectral and hyperspectral imaging systems for digitisation of a Russian icon

In a study of multispectral and hyperspectral reflectance imaging, a Round Robin Test assessed the performance of different systems for the spectral digitisation of artworks. A Russian icon, mass-produced in Moscow in 1899, was digitised by ten institutions around Europe. The image quality was assessed by observers, and the reflectance spectra at selected points were reconstructed to characterise the icon’s colourants and to obtain a quantitative estimate of accuracy. The differing spatial resolutions of the systems affected their ability to resolve fine details in the printed pattern. There was a surprisingly wide variation in the quality of imagery, caused by unwanted reflections from both glossy painted and metallic gold areas of the icon’s surface. Specular reflection also degraded the accuracy of the reconstructed reflectance spectrum in some places, indicating the importance of control over the illumination geometry. Some devices that gave excellent results for matte colour charts proved to have poor performance for this demanding test object. There is a need for adoption of standards for digitising cultural heritage objects to achieve greater consistency of system performance and image quality.This article arose out of a Short-Term Scientific Mission (STSM) conducted by Tatiana Vitorino when visiting University College London during a 2-week period in late October 2015. The research was carried out under the auspices of the European COST Action TD1201 Colour and Space in Cultural Heritage (COSCH). The project website is at http://www.cosch.info. Under the COST rules, TV received funding for travel and accommodation expenses, and all coauthors were able to claim travel expenses to attend the subsequent COSCH project meeting. No other funding was received from COSCH for labour or equipment and all work was done on a voluntary pro bono basis.info:eu-repo/semantics/publishedVersio

Detection of antibodies to human herpesvirus 8 in Italian children: evidence for horizontal transmission

Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma associated herpesvirus (KSHV), has been shown to be the causative agent for Kaposi's sarcoma (KS) and to be more prevalent in populations or risk groups at increased risk for KS. HHV-8 infection is rare in children from the US and the UK, but has been reported in African children. In this study we examine HHV-8 infection in children from Italy, a country with an elevated prevalence of HHV-8 in adults and high socio-economic conditions. © 2000 Cancer Research Campaig

A tunable nanoplatform of nanogold functionalised with Angiogenin peptides for anti-angiogenic therapy of brain tumours

Angiogenin (ANG), an endogenous protein that plays a key role in cell growth and survival, has been scrutinised here as promising nanomedicine tool for the modulation of pro-/anti-angiogenic processes in brain cancer therapy. Specifically, peptide fragments from the putative cell membrane binding domain (residues 60-68) of the protein were used in this study to obtain peptide-functionalised spherical gold nanoparticles (AuNPs) of about 10 nm and 30 nm in optical and hydrodynamic size, respectively. Different hybrid biointerfaces were fabricated by peptide physical adsorption (Ang60-68) or chemisorption (the cysteine analogous Ang60-68Cys) at the metal nanoparticle surface, and cellular assays were performed in the comparison with ANG-functionalised AuNPs. Cellular treatments were performed both in basal and in copper-supplemented cell culture medium, to scrutinise the synergic effect of the metal, which is another known angiogenic factor. Two brain cell lines were investigated in parallel, namely tumour glioblastoma (A172) and neuron-like differentiated neuroblastoma (d-SH-SY5Y). Results on cell viability/proliferation, cytoskeleton actin, angiogenin translocation and vascular endothelial growth factor (VEGF) release pointed to the promising potentialities of the developed systems as anti-angiogenic tunable nanoplaftforms in cancer cells treatment

Graphene Oxide Nanosheets Tailored With Aromatic Dipeptide Nanoassemblies for a Tuneable Interaction With Cell Membranes

Engineered graphene-based derivatives are attractive and promising candidates for nanomedicine applications because of their versatility as 2D nanomaterials. However, the safe application of these materials needs to solve the still unanswered issue of graphene nanotoxicity. In this work, we investigated the self-assembly of dityrosine peptides driven by graphene oxide (GO) and/or copper ions in the comparison with the more hydrophobic diphenylalanine dipeptide. To scrutinize the peptide aggregation process, in the absence or presence of GO and/or Cu2+, we used atomic force microscopy, circular dichroism, UV–visible, fluorescence and electron paramagnetic resonance spectroscopies. The perturbative effect by the hybrid nanomaterials made of peptide-decorated GO nanosheets on model cell membranes of supported lipid bilayers was investigated. In particular, quartz crystal microbalance with dissipation monitoring and fluorescence recovery after photobleaching techniques were used to track the changes in the viscoelastic properties and fluidity of the cell membrane, respectively. Also, cellular experiments with two model tumour cell lines at a short time of incubation, evidenced the high potential of this approach to set up versatile nanoplatforms for nanomedicine and theranostic applications

Copy number variations residing outside the SHOX enhancer region are involved in Short Stature and Léri-Weill dyschondrosteosis

Background: SHOX enhancer CNVs, affecting one or more of the seven recognized evolutionary conserved non-coding elements (CNEs) represent one of the most frequent cause of SHOX-haploinsufficiency. During the diagnostic workflow deletions/duplications have been identified downstream SHOX not including any of the these CNEs. Methods: Fine tiling aCGH and breakpoint PCR were used to characterize the critical interval and to search for novel alterations in a cohort of selected patients. Results: Screening of 252 controls provided evidence that duplications in this area represent likely benign variants whereas none of the deletions were detected. These findings suggested that other alterations relevant for SHOX-haploinsufficiency might be missed by the standard diagnostic methods. To identify such undisclosed elements, the aCGH was used to reanalyze 52 unresolved cases with clinical features strongly suggestive of SHOX-haploinsufficiency. This analysis followed by the screening of 210 patients detected two partially overlapping small deletions of ~12 and ~8 kb in four unrelated individuals, approximately 15 kb downstream SHOX, that were absent in 720 normal stature individuals. Conclusion: Our results strengthen the hypothesis that alterations of yet unidentified cis-regulatory elements residing outside those investigated through conventional methods, might explain the phenotype in ISS/LWD patients thus enlarging the spectrum of variants contributing to SHOX-haploinsufficiency

Long-term expression of the Paganica Fault vs. 2009 L’Aquila Earthquake surface ruptures: looking for a better understanding of its seismic behavior

The Mw6.3, April 6, 2009 earthquake occurred on the previously identified Paganica normal fault and produced a 3 km-long co-seismic surface rupture along its northern section, with few centimeters of vertical displacement. Extensive 1:10,000-scale geological and geomorphological mapping has been carried out, focusing on the characterization of the long-term expression of the Paganica Fault at the surface. The field mapping was integrated by observations, made on 1:33,000 scale aerial photographs (GAI), 5-m-resolution Digital Elevation Model and standard morphometric derivatives (hill-shaded and slope angle maps, Spatial Analyst™). Particular attention was devoted to the study of the continental deposits and landforms affected by cumulative offset with the aim to reconstruct the Quaternary deformational history of the fault. The fault runs for a total length of 20 km and, along with antithetic faults on its hanging-wall, forms the graben of the Middle Aterno River Valley. The whole fault system and the variable setting of deformation affecting the continental deposits at the surface were identified. The Paganica long-term morphologic signature is represented by a set of prominent scarps formed by the tectonic juxtaposition of late Pliocene-middle Pleistocene and late Pleistocene alluvial deposits, and by lower scarps in late Pleistocene-Holocene deposits. In addition, evident Quaternary erosional and depositional paleosurfaces were recognized and sampled for 14C and OSL (Optically Stimulated Luminescence) and tephra chronology dating for long-term slip-rate calculations. This study resulted helpful to locate four paleoseismological investigations (see Pantosti et al. talk) and to provide the appropriate context for correctly interpret the depositional bodies outcropping on the trench walls. These paleoseismological investigations evidenced the presence of repeated late Pleistocene-Holocene activity and allowed for slip-rate estimation at a shorter time-scale. Such estimates were valuable for a comparison with the preliminary estimates on late Pleistocene calculations carried out by geomorphological investigations. Moreover, we correlated co-seismic deformations with the long-term morphologies and structures. The 2009 co-seismic ruptures show a general coherence with the long-term Paganica fault trace, both in terms of location and style. However, the limited extent of the 2009 surface ruptures coincides with the portion of the fault trace where deformation is more localized and few splays contribute to the extension. This is also testified by the presence on its hanging-wall of a large late Pleistocene-Holocene alluvial fan that subsides over the basin depocenter. Conversely, where the Paganica fault system branches out, various splays accommodated the small 2009 co-seismic throw, resulting in a distributed and not evident extensional strain. The preserved fault-related geomorphology is evidence for the persistence of the rupture complexities during Quaternary. On this light, further studies on the style of fault activity are needed to estimate if the Paganica fault is capable of earthquakes with Magnitude larger than the 2009 event.SubmittedVienna, Austriaope